SRR2936474.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936474.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	622624
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	3521	0.565509842216169	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2645	0.4248149766151	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	1938	0.31126329855578966	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1242	0.1994783368453513	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1014	0.1628591252505525	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	946	0.15193760600298092	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	942	0.15129516369430024	No Hit
ACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	939	0.15081333196278973	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	848	0.13619776944030426	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	50	2.1827873E-11	45.000004	1
CGGGTCA	55	1.8189894E-12	45.000004	6
GTACACG	50	2.1827873E-11	45.000004	1
GTACTAG	50	2.1827873E-11	45.000004	1
TCGTTGA	20	7.030793E-4	45.0	24
CCCCATA	20	7.030793E-4	45.0	31
CTCGACG	20	7.030793E-4	45.0	1
GCGCTAA	20	7.030793E-4	45.0	9
GCGTAAG	20	7.030793E-4	45.0	1
CCCGCGT	20	7.030793E-4	45.0	20
TGCCGAT	20	7.030793E-4	45.0	11
GCTCACG	20	7.030793E-4	45.0	44
ACGTTGT	30	2.1638934E-6	44.999996	20
CGGTCTA	30	2.1638934E-6	44.999996	31
TATCGCG	30	2.1638934E-6	44.999996	1
AGTCGTT	30	2.1638934E-6	44.999996	23
CTCGCGG	55	6.002665E-11	40.909092	2
ACAACGG	50	1.0804797E-9	40.500004	2
CGTTCGT	45	1.925946E-8	40.000004	41
CGGGATC	85	0.0	39.705883	6