##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936474.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	622624
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01128449915198	33.0	31.0	34.0	30.0	34.0
2	32.01254047386545	33.0	31.0	34.0	30.0	34.0
3	32.06420568432955	33.0	31.0	34.0	30.0	34.0
4	35.73831076219356	37.0	35.0	37.0	33.0	37.0
5	34.82753957444621	37.0	35.0	37.0	33.0	37.0
6	35.315630300149046	37.0	35.0	37.0	33.0	37.0
7	35.874050791488926	37.0	35.0	37.0	35.0	37.0
8	35.85383955645783	37.0	35.0	37.0	35.0	37.0
9	37.69079251683199	39.0	37.0	39.0	35.0	39.0
10	37.37600542221308	39.0	37.0	39.0	34.0	39.0
11	37.29124319011153	39.0	37.0	39.0	34.0	39.0
12	37.13814758184715	39.0	37.0	39.0	34.0	39.0
13	36.99542581076219	39.0	37.0	39.0	33.0	39.0
14	37.96537235956211	40.0	37.0	41.0	33.0	41.0
15	38.15635118466362	40.0	37.0	41.0	33.0	41.0
16	38.19883910674822	40.0	37.0	41.0	34.0	41.0
17	38.213801588117384	40.0	37.0	41.0	34.0	41.0
18	38.207976242483426	40.0	37.0	41.0	34.0	41.0
19	38.154648712545615	40.0	37.0	41.0	34.0	41.0
20	38.1535372873516	40.0	37.0	41.0	34.0	41.0
21	38.052371897003646	40.0	36.0	41.0	34.0	41.0
22	38.09898751092152	40.0	36.0	41.0	34.0	41.0
23	38.10194113943568	40.0	36.0	41.0	34.0	41.0
24	38.059154481677545	40.0	36.0	41.0	34.0	41.0
25	37.884822942899724	40.0	36.0	41.0	34.0	41.0
26	37.895530850079666	40.0	36.0	41.0	34.0	41.0
27	37.90753167240582	40.0	36.0	41.0	34.0	41.0
28	37.80674371691422	40.0	36.0	41.0	34.0	41.0
29	37.780565798941254	40.0	36.0	41.0	33.0	41.0
30	37.68272504754073	40.0	36.0	41.0	33.0	41.0
31	37.634045587706225	40.0	36.0	41.0	33.0	41.0
32	37.608571144061266	40.0	36.0	41.0	33.0	41.0
33	37.48956191858971	40.0	35.0	41.0	33.0	41.0
34	37.4254333273372	40.0	35.0	41.0	33.0	41.0
35	37.370645847252916	40.0	35.0	41.0	33.0	41.0
36	37.29810286786247	40.0	35.0	41.0	33.0	41.0
37	37.26676935036234	40.0	35.0	41.0	33.0	41.0
38	37.21350606465539	40.0	35.0	41.0	32.0	41.0
39	37.16660617001593	39.0	35.0	41.0	32.0	41.0
40	37.0536856915249	39.0	35.0	41.0	32.0	41.0
41	36.96410353600247	39.0	35.0	41.0	32.0	41.0
42	36.961280002055815	39.0	35.0	41.0	32.0	41.0
43	36.84703448630313	39.0	35.0	41.0	31.0	41.0
44	36.711262334892325	39.0	35.0	41.0	31.0	41.0
45	36.71003366397697	39.0	35.0	41.0	31.0	41.0
46	36.59047675643727	39.0	35.0	41.0	31.0	41.0
47	36.495984735570744	39.0	35.0	41.0	31.0	41.0
48	36.4656229120625	39.0	35.0	41.0	31.0	41.0
49	36.44961806804749	39.0	35.0	41.0	31.0	41.0
50	36.33018483065221	39.0	35.0	40.0	31.0	41.0
51	35.091739155573826	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	13.0
10	12.0
11	13.0
12	17.0
13	13.0
14	9.0
15	21.0
16	38.0
17	70.0
18	120.0
19	209.0
20	387.0
21	636.0
22	1073.0
23	1555.0
24	2259.0
25	3097.0
26	4074.0
27	4857.0
28	5745.0
29	6975.0
30	8872.0
31	11435.0
32	15267.0
33	22212.0
34	41459.0
35	54343.0
36	44946.0
37	67181.0
38	119953.0
39	205619.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96160122321015	21.376625379040963	24.906685254664133	11.75508814308475
2	31.184631495091743	24.67861823508249	26.071433160302206	18.065317109523566
3	31.84297425091227	23.624209795960322	27.810363879323635	16.722452073803773
4	27.79285732641209	25.830838515701288	26.796268695071184	19.58003546281544
5	24.450229994346508	31.284370663514416	25.45211877473403	18.813280567405048
6	25.384019890013874	32.99680063730277	26.299018348152337	15.320161124531017
7	76.5323855167806	6.600612889962481	10.859041733052372	6.007959860204553
8	76.50797270905073	6.222535591303901	10.436635915094824	6.832855784550547
9	70.67829058950507	7.897543300611605	13.314938068561444	8.10922804132189
10	40.338791951482754	23.76458344040705	20.199189237806443	15.697435370303747
11	29.78507092563088	25.322506038957705	25.411002466978466	19.481420568432956
12	27.35406922958318	21.899091586575526	31.428598961813233	19.318240222028063
13	24.729531788045435	25.047701341419543	31.58551549570848	18.63725137482654
14	19.496999794418464	28.999203371537234	30.507497301742305	20.996299532301997
15	17.354775916122733	25.438627486251736	37.240132086138665	19.966464511486866
16	20.08403145397543	25.45950686128386	32.37106182864778	22.08539985609292
17	20.69595775299378	25.375507529423857	28.229718096314947	25.69881662126741
18	22.24392249575988	24.588033869558515	31.7711813229172	21.3968623117644
19	22.83111476589402	26.625700262116464	27.99169322094876	22.551491751040757
20	22.978234054581897	27.575069383769335	28.345357711877472	21.101338849771288
21	22.703911188775248	27.111707868633395	29.958369738397494	20.226011204193863
22	21.064559027599323	25.438627486251736	28.901712751194943	24.595100734954002
23	19.603163385927942	26.552461838926867	29.74957598807627	24.09479878706892
24	22.448540371074678	23.718327594182043	30.427834455465902	23.40529757927738
25	19.733257953435782	26.944512257799246	28.62321401038187	24.699015778383103
26	18.64753045176543	28.65613917870175	28.409601942745542	24.286728426787274
27	19.7632921313666	26.760773757516574	29.123837179421287	24.352096931695534
28	16.88258081924243	26.723833324767437	30.25052037827003	26.1430654777201
29	18.496395898648302	26.224976872076887	29.554594747391683	25.724032481883125
30	20.276282314848125	25.19032353394665	29.648391324459062	24.88500282674616
31	20.489251940175773	26.58635067070977	25.890746260985765	27.033651128128692
32	21.077247263195765	27.07814025800483	25.91258929948091	25.9320231793185
33	20.43994449298453	25.29054453410084	27.763786811944286	26.505724160970345
34	17.79677622449504	25.357358534203627	28.909261448321942	27.93660379297939
35	17.24829110345891	26.111585804594746	29.633936372513748	27.006186719432595
36	20.678451200082232	26.023410597728326	28.55431207277587	24.74382612941358
37	18.172604975073238	25.957560261088553	29.463367939559028	26.40646682427918
38	18.256764917510406	26.48179318497199	28.53295086601223	26.72849103150537
39	19.52382176080588	24.880023898853885	29.67119802641723	25.92495631392301
40	21.004008840006165	23.282591098319372	31.066582720871665	24.646817340802794
41	17.960920234362952	25.29439918795292	28.434978413938428	28.309702163745698
42	19.511133525209438	25.390604923677856	29.34226756437272	25.755993986739988
43	20.76084442617053	24.37924011923729	28.769690856761066	26.090224597831114
44	19.909608367168627	24.298131777766358	28.16226165390348	27.629998201161538
45	19.25769003443491	23.50150331500231	27.87942642750681	29.36138022305597
46	21.332296859741994	24.22730251323431	29.315927429716808	25.12447319730688
47	17.449696767230304	23.8644832194069	32.248997789998455	26.43682222336434
48	18.63628771136352	23.79526006064655	30.193343012797452	27.375109215192477
49	19.889532045022356	21.75181168731048	32.628520583851575	25.73013568381559
50	19.375417587500642	21.69431310068356	31.49364624556715	27.43662306624865
51	18.377704682119543	22.37642622192527	28.642647890219457	30.603221205735725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	282.0
1	468.0
2	654.0
3	1342.5
4	2031.0
5	1427.0
6	823.0
7	828.5
8	834.0
9	924.0
10	1014.0
11	1044.0
12	1074.0
13	1116.0
14	1158.0
15	1158.0
16	1158.0
17	1088.0
18	1018.0
19	1418.5
20	1819.0
21	1844.0
22	1869.0
23	2239.0
24	2609.0
25	3185.0
26	4403.5
27	5046.0
28	6043.5
29	7041.0
30	8479.0
31	9917.0
32	11353.5
33	12790.0
34	14645.5
35	16501.0
36	17860.5
37	19220.0
38	21164.0
39	23108.0
40	25443.0
41	27778.0
42	32715.5
43	37653.0
44	41668.0
45	45683.0
46	53684.0
47	61685.0
48	68172.5
49	74660.0
50	72640.0
51	70620.0
52	58592.0
53	46564.0
54	40134.0
55	33704.0
56	30198.0
57	26692.0
58	25011.0
59	23330.0
60	21127.5
61	18925.0
62	16007.0
63	13089.0
64	11296.0
65	9503.0
66	8551.0
67	7599.0
68	6231.5
69	4864.0
70	3699.0
71	2534.0
72	2184.5
73	1835.0
74	1463.0
75	838.5
76	586.0
77	403.5
78	221.0
79	202.0
80	183.0
81	125.5
82	68.0
83	41.5
84	15.0
85	11.0
86	7.0
87	5.0
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	622624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.329054649155914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.40244913572512	22.369712310567074
2	12.006840101201774	7.523258993884541
3	4.692111034117518	4.409982090233256
4	2.2612571101185166	2.833721903147815
5	1.3546544446660695	2.122002156383262
6	0.9469776645268354	1.7800749002094756
7	0.6333639024216987	1.3889884618240436
8	0.5005159605907962	1.2544553505699054
9	0.3977609279717338	1.1215326464752875
>10	5.275110172655295	40.700628015984016
>50	0.4686395977760868	8.933752138434812
>100	0.053617731758719755	2.936084076808641
>500	0.004124440904516904	0.9524748487699722
>1k	0.002577775565323065	1.6733321067079723
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	3521	0.565509842216169	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2645	0.4248149766151	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	1938	0.31126329855578966	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1242	0.1994783368453513	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1014	0.1628591252505525	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	946	0.15193760600298092	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	942	0.15129516369430024	No Hit
ACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	939	0.15081333196278973	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	848	0.13619776944030426	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.424423086806804E-4	0.0	0.0	0.3286092408901681	0.0
2	6.424423086806804E-4	0.0	0.0	1.7463188055712597	0.0
3	8.030528858508506E-4	0.0	0.0	2.396470421956108	0.0
4	8.030528858508506E-4	0.0	0.0	3.1966323174178957	0.0
5	8.030528858508506E-4	0.0	0.0	5.265617772524028	0.0
6	9.636634630210207E-4	0.0	0.0	6.340263144369636	0.0
7	9.636634630210207E-4	0.0	0.0	7.474976872076888	0.0
8	9.636634630210207E-4	0.0	0.0	9.219368350722105	0.0
9	9.636634630210207E-4	0.0	0.0	9.887508351750013	0.0
10	9.636634630210207E-4	0.0	0.0	11.413790666598139	0.0
11	9.636634630210207E-4	0.0	0.0	13.353002775350774	0.0
12	9.636634630210207E-4	0.0	0.0	14.96392686436758	0.0
13	9.636634630210207E-4	0.0	0.0	15.603638793236367	0.0
14	9.636634630210207E-4	0.0	0.0	15.894183327337204	0.0
15	9.636634630210207E-4	0.0	0.0	16.400909698309093	0.0
16	9.636634630210207E-4	0.0	0.0	17.379831166161278	0.0
17	9.636634630210207E-4	0.0	0.0	18.51502672560004	0.0
18	9.636634630210207E-4	0.0	0.0	19.824002929536928	0.0
19	0.0011242740401911908	0.0	0.0	20.556868993164414	0.0
20	0.0011242740401911908	0.0	0.0	21.284916739476795	0.0
21	0.0011242740401911908	0.0	0.0	22.171165904301795	0.0
22	0.0011242740401911908	0.0	0.0	23.070585136454746	0.0
23	0.0011242740401911908	0.0	0.0	23.939167137791028	0.0
24	0.0011242740401911908	0.0	0.0	24.624653081153312	0.0
25	0.0011242740401911908	0.0	0.0	25.24653723595621	0.0
26	0.0011242740401911908	0.0	0.0	25.84946934265303	0.0
27	0.0011242740401911908	0.0	0.0	26.4623593051344	0.0
28	0.0012848846173613608	0.0	0.0	27.06770057048877	0.0
29	0.0012848846173613608	0.0	0.0	27.683320912782033	0.0
30	0.0012848846173613608	0.0	0.0	28.382779976358123	0.0
31	0.0012848846173613608	0.0	0.0	28.99117284267873	0.0
32	0.0012848846173613608	0.0	0.0	29.565998098370766	0.0
33	0.0012848846173613608	0.0	0.0	30.198482551266896	0.0
34	0.0012848846173613608	0.0	0.0	30.792420465642184	0.0
35	0.0012848846173613608	0.0	0.0	31.45750886570386	0.0
36	0.0012848846173613608	0.0	0.0	32.02012771753097	0.0
37	0.0012848846173613608	0.0	0.0	32.583228401089585	0.0
38	0.0012848846173613608	0.0	0.0	33.14745335868839	0.0
39	0.0012848846173613608	0.0	0.0	33.872288893457366	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	50	2.1827873E-11	45.000004	1
CGGGTCA	55	1.8189894E-12	45.000004	6
GTACACG	50	2.1827873E-11	45.000004	1
GTACTAG	50	2.1827873E-11	45.000004	1
TCGTTGA	20	7.030793E-4	45.0	24
CCCCATA	20	7.030793E-4	45.0	31
CTCGACG	20	7.030793E-4	45.0	1
GCGCTAA	20	7.030793E-4	45.0	9
GCGTAAG	20	7.030793E-4	45.0	1
CCCGCGT	20	7.030793E-4	45.0	20
TGCCGAT	20	7.030793E-4	45.0	11
GCTCACG	20	7.030793E-4	45.0	44
ACGTTGT	30	2.1638934E-6	44.999996	20
CGGTCTA	30	2.1638934E-6	44.999996	31
TATCGCG	30	2.1638934E-6	44.999996	1
AGTCGTT	30	2.1638934E-6	44.999996	23
CTCGCGG	55	6.002665E-11	40.909092	2
ACAACGG	50	1.0804797E-9	40.500004	2
CGTTCGT	45	1.925946E-8	40.000004	41
CGGGATC	85	0.0	39.705883	6
>>END_MODULE