FastQCFastQC Report
Sat 14 Jan 2017
SRR2936473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643335
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC48980.7613451778622335TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC45730.7108271740228652TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC39300.6108792464268227No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC35040.544661801394297No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCG34970.5435737213116028No Hit
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT32030.4978743578384512TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC20110.3125898637568297TruSeq Adapter, Index 21 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC14060.2185486566096979TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.1786005735736436No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG9100.1414504107502312No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG8710.13538825028950702No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCC8080.12559552954526024No Hit
TGTTTTCTAATTGGTTATAACATTTTTTCAATTAATAGTTTCAAAACAAAT8060.12528464952163337No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG7620.11844528900184195No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCT7390.11487016873013282No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG6970.10834168823396831No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT6590.10243496778505755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG351.2108103E-745.0000041
AGTACGG600.045.0000042
TCCGGCC207.0309686E-445.041
CTATGCG207.0309686E-445.01
TAATACG253.8888837E-545.04
GGTACCG207.0309686E-445.09
ACACGAC207.0309686E-445.026
GATCGCG253.8888837E-545.01
GGTCGAC207.0309686E-445.08
GACGTAC207.0309686E-445.010
CGTCTAG207.0309686E-445.023
TCTAGCG207.0309686E-445.01
GTCAGGT207.0309686E-445.03
CTAACGG207.0309686E-445.02
CCCCCTA207.0309686E-445.021
TTGCGGT207.0309686E-445.03
TACTACG800.045.01
CCGGTCG207.0309686E-445.023
TATCGCG207.0309686E-445.01
AGTACGT207.0309686E-445.019
Produced by FastQC (version 0.11.5)