##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936473.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	643335
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.897945860243887	33.0	31.0	34.0	30.0	34.0
2	31.94218564200611	33.0	31.0	34.0	30.0	34.0
3	31.98600107253608	33.0	31.0	34.0	30.0	34.0
4	35.65600348185627	37.0	35.0	37.0	33.0	37.0
5	34.736264931956136	37.0	35.0	37.0	33.0	37.0
6	35.24804806205165	37.0	35.0	37.0	33.0	37.0
7	35.844120092953126	37.0	35.0	37.0	35.0	37.0
8	35.83863772373647	37.0	35.0	37.0	35.0	37.0
9	37.69119199173059	39.0	37.0	39.0	35.0	39.0
10	37.322494501309585	39.0	37.0	39.0	34.0	39.0
11	37.246863609161636	39.0	37.0	39.0	34.0	39.0
12	37.13220483884757	39.0	37.0	39.0	34.0	39.0
13	36.994973070017956	39.0	37.0	39.0	33.0	39.0
14	37.99840829427903	40.0	37.0	41.0	33.0	41.0
15	38.1361157095448	40.0	37.0	41.0	33.0	41.0
16	38.191261162535845	40.0	37.0	41.0	34.0	41.0
17	38.18744355584571	40.0	37.0	41.0	34.0	41.0
18	38.19973264317968	40.0	37.0	41.0	34.0	41.0
19	38.13807736249388	40.0	37.0	41.0	34.0	41.0
20	38.1108986764283	40.0	37.0	41.0	34.0	41.0
21	38.03310405931591	40.0	36.0	41.0	34.0	41.0
22	38.10492356237419	40.0	37.0	41.0	34.0	41.0
23	38.12411418623268	40.0	37.0	41.0	34.0	41.0
24	38.10425827912363	40.0	36.0	41.0	34.0	41.0
25	37.97077572337896	40.0	36.0	41.0	34.0	41.0
26	38.001653881725694	40.0	36.0	41.0	34.0	41.0
27	38.04625272991521	40.0	36.0	41.0	34.0	41.0
28	37.94877318970676	40.0	36.0	41.0	34.0	41.0
29	37.91570954479393	40.0	36.0	41.0	34.0	41.0
30	37.82735588767905	40.0	36.0	41.0	34.0	41.0
31	37.77421716524051	40.0	36.0	41.0	33.0	41.0
32	37.69933083074914	40.0	36.0	41.0	33.0	41.0
33	37.58455237162598	40.0	36.0	41.0	33.0	41.0
34	37.50964116673273	40.0	36.0	41.0	33.0	41.0
35	37.47047650135622	40.0	36.0	41.0	33.0	41.0
36	37.427960549325	40.0	36.0	41.0	33.0	41.0
37	37.35780114559289	40.0	35.0	41.0	33.0	41.0
38	37.26801899476944	40.0	35.0	41.0	33.0	41.0
39	37.236704049989505	40.0	35.0	41.0	33.0	41.0
40	37.12228310289352	39.0	35.0	41.0	32.0	41.0
41	37.044236672961986	39.0	35.0	41.0	32.0	41.0
42	37.103512167066924	39.0	35.0	41.0	32.0	41.0
43	37.00383936829179	39.0	35.0	41.0	32.0	41.0
44	36.87213970948262	39.0	35.0	41.0	31.0	41.0
45	36.854245455322655	39.0	35.0	41.0	31.0	41.0
46	36.72522402791703	39.0	35.0	41.0	31.0	41.0
47	36.61933362866935	39.0	35.0	41.0	31.0	41.0
48	36.58939899119432	39.0	35.0	41.0	31.0	41.0
49	36.61809788057544	39.0	35.0	41.0	31.0	41.0
50	36.50809298421507	39.0	35.0	41.0	31.0	41.0
51	35.255980165854496	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	3.0
10	4.0
11	7.0
12	8.0
13	11.0
14	9.0
15	13.0
16	30.0
17	60.0
18	100.0
19	219.0
20	393.0
21	753.0
22	1106.0
23	1584.0
24	2430.0
25	3306.0
26	4114.0
27	4779.0
28	5653.0
29	6869.0
30	8746.0
31	11466.0
32	15416.0
33	22719.0
34	40081.0
35	53322.0
36	46923.0
37	70947.0
38	129140.0
39	212995.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.521866523661856	22.43543410509299	26.36418040367771	11.678518967567442
2	29.42619319639068	27.12583646156357	25.989880855230947	17.458089486814803
3	29.373499032385926	26.472366651899865	27.65852938204823	16.495604933665973
4	27.5910684169212	24.66149051427328	30.03147660239222	17.7159644664133
5	24.312216807728475	29.57432752764889	26.775474674936074	19.337980989686553
6	24.163927036458453	33.69333240069326	26.290968158113582	15.851772404734701
7	76.08726402263206	5.56366434283849	12.183699005961124	6.16537262856832
8	75.83063256312808	6.150605827446043	10.660076010165778	7.358685599260105
9	69.9536011564737	7.010033652762558	13.475094624107193	9.561270566656562
10	38.71249038214927	26.043973979342024	20.504091958310987	14.73944368019772
11	28.165885580607302	24.80480620516527	27.508685210660076	19.520623003567348
12	24.91858829381271	21.199219691140698	33.40094973847218	20.481242276574413
13	21.785850295724625	25.885425167292315	34.26535164416673	18.06337289281634
14	17.643373980896424	29.933549394949754	31.002199476167156	21.420877147986666
15	15.902601288597698	25.59024458485859	38.998500003886	19.508654122657713
16	17.64912526133352	27.829668835054832	31.81359633783332	22.707609565778327
17	18.301506990914532	26.436304569159148	29.387177753425508	25.87501068650081
18	20.00139896010632	25.80972588153917	32.698205445063614	21.490669713290895
19	20.424972992297945	27.723503306986252	29.232514941671134	22.619008759044664
20	21.178546169569508	28.19324302268647	30.285931901730823	20.3422789060132
21	20.210621216007212	29.03308540651449	31.259297255706592	19.496996121771705
22	18.346740034352244	25.997963735845243	29.41655591565825	26.238740314144266
23	17.178608345574233	27.00381605229002	30.45613871466654	25.361436887469203
24	20.64958380936837	24.72211211888052	30.478211196344052	24.150092875407058
25	17.715653586389674	27.90754428097337	29.115624052787425	25.261178079849532
26	16.915759285597705	26.89625156411512	32.05639363628592	24.13159551400126
27	19.571141007406716	26.81542275797213	31.75701617353323	21.856420061087924
28	17.363581959632228	25.37620368859148	33.68664848018528	23.573565871591008
29	17.646171901109064	22.661599322281546	35.98545081489426	23.706777961715126
30	20.498806997909334	24.33941880979583	34.90996137315707	20.251812819137776
31	24.104393511933907	25.270504480558344	28.583552892350017	22.041549115157732
32	21.29434897837052	24.868225729985156	31.085981642534605	22.751443649109717
33	20.373366908375885	23.313670171838933	31.517793995352346	24.79516892443284
34	22.045435115453067	21.796420216527938	32.582091756238974	23.57605291178002
35	17.700109585208327	21.840720619894768	33.40374765868482	27.05542213621208
36	21.905383664809158	21.381706265009676	33.748824484910664	22.964085585270507
37	21.323571700591447	22.494190429558472	35.08187802622273	21.10035984362735
38	19.912487273349033	23.625793715560324	30.654635609752308	25.80708340133834
39	21.74870013290121	21.42756106849464	33.18846324232321	23.635275556280945
40	22.61279115857213	20.147978891246396	33.61219271452664	23.62703723565483
41	19.66129621425851	22.373724420403057	28.506454646490553	29.45852471884788
42	20.769583498488345	19.858860469273395	31.848104020455903	27.52345201178235
43	22.131393441985903	20.365284027761586	31.942300667614848	25.561021862637663
44	20.2521236991614	21.93662710718366	32.07038323734913	25.740865956305814
45	20.492123077401352	20.84015326385165	31.33033334110533	27.337390317641663
46	24.279263525224028	21.42880458858915	31.56053999860104	22.731391887585783
47	18.383112997116587	21.215385452369297	35.81213520172227	24.58936634879184
48	19.31699658809174	20.73958357620835	32.42245486410657	27.520964971593337
49	21.36149906347393	17.956741044712317	35.646125269105525	25.03563462270823
50	20.909168629096815	18.557827570394895	33.6578920779998	26.875111722508493
51	19.240675542291342	18.277724669107076	29.973652917997622	32.50794687060396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	411.0
1	492.0
2	573.0
3	1398.5
4	2224.0
5	1596.0
6	968.0
7	1018.0
8	1068.0
9	1162.5
10	1257.0
11	1312.0
12	1367.0
13	1363.5
14	1360.0
15	1325.5
16	1291.0
17	1814.5
18	2338.0
19	1952.5
20	1567.0
21	2012.0
22	2457.0
23	2535.5
24	2614.0
25	2931.0
26	3985.0
27	4722.0
28	5747.0
29	6772.0
30	7634.0
31	8496.0
32	10616.0
33	12736.0
34	14181.0
35	15626.0
36	17777.5
37	19929.0
38	21586.0
39	23243.0
40	26967.0
41	30691.0
42	35177.5
43	39664.0
44	46084.5
45	52505.0
46	69890.0
47	87275.0
48	89383.5
49	91492.0
50	86181.0
51	80870.0
52	63904.0
53	46938.0
54	37611.0
55	28284.0
56	24027.5
57	19771.0
58	17506.5
59	15242.0
60	13387.0
61	11532.0
62	9987.0
63	8442.0
64	7226.5
65	6011.0
66	4880.0
67	3749.0
68	3232.5
69	2716.0
70	2186.5
71	1657.0
72	1297.0
73	937.0
74	794.5
75	495.0
76	338.0
77	242.5
78	147.0
79	121.0
80	95.0
81	54.5
82	14.0
83	15.5
84	17.0
85	18.5
86	20.0
87	12.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	643335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.12626391878961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.09140062159128	22.196485708880655
2	13.192786636494336	8.476698906165955
3	5.34481007045934	5.151263367581437
4	2.7884222218233035	3.5832635286125263
5	1.6178154546593844	2.5987183134142
6	1.0455700625585553	2.015415586520491
7	0.772358105039762	1.7369086225626538
8	0.5616254238732618	1.4434341272683593
9	0.4350434855928244	1.2578689650874701
>10	4.739092207805995	35.03780342992124
>50	0.337305334736618	6.638245118344079
>100	0.0616370899433638	3.791010964286156
>500	0.007765302670030085	1.6804933854189432
>1k	0.004367982751891922	4.392389975935893
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	4898	0.7613451778622335	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	4573	0.7108271740228652	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC	3930	0.6108792464268227	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC	3504	0.544661801394297	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCG	3497	0.5435737213116028	No Hit
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT	3203	0.4978743578384512	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	2011	0.3125898637568297	TruSeq Adapter, Index 21 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	1406	0.2185486566096979	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1149	0.1786005735736436	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG	910	0.1414504107502312	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG	871	0.13538825028950702	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCC	808	0.12559552954526024	No Hit
TGTTTTCTAATTGGTTATAACATTTTTTCAATTAATAGTTTCAAAACAAAT	806	0.12528464952163337	No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG	762	0.11844528900184195	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCT	739	0.11487016873013282	No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTG	697	0.10834168823396831	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	659	0.10243496778505755	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.108800236268818E-4	0.0	0.0	0.523211079764042	0.0
2	3.108800236268818E-4	0.0	0.0	2.6306667599306737	0.0
3	3.108800236268818E-4	0.0	0.0	3.568591791212976	0.0
4	3.108800236268818E-4	0.0	0.0	4.793148204279263	0.0
5	3.108800236268818E-4	0.0	0.0	7.953243644446517	0.0
6	3.108800236268818E-4	0.0	0.0	9.479042800407253	0.0
7	3.108800236268818E-4	0.0	0.0	11.12048932515719	0.0
8	3.108800236268818E-4	0.0	0.0	13.58887671275463	0.0
9	3.108800236268818E-4	0.0	0.0	14.452501418390108	0.0
10	4.663200354403227E-4	0.0	0.0	16.883738643164136	0.0
11	4.663200354403227E-4	0.0	0.0	19.53150380439429	0.0
12	4.663200354403227E-4	0.0	0.0	22.20864712785718	0.0
13	4.663200354403227E-4	0.0	0.0	23.128074797733685	0.0
14	4.663200354403227E-4	0.0	0.0	23.495379545648845	0.0
15	4.663200354403227E-4	0.0	0.0	24.129730233859497	0.0
16	4.663200354403227E-4	0.0	0.0	25.381333208981324	0.0
17	4.663200354403227E-4	0.0	0.0	26.91117380524921	0.0
18	9.326400708806454E-4	0.0	0.0	28.694070740749375	0.0
19	9.326400708806454E-4	0.0	0.0	29.805311385203666	0.0
20	9.326400708806454E-4	0.0	0.0	30.875982186574646	0.0
21	9.326400708806454E-4	0.0	0.0	32.03121235437214	0.0
22	9.326400708806454E-4	0.0	0.0	33.245043406623296	0.0
23	9.326400708806454E-4	0.0	0.0	34.378667412778725	0.0
24	0.0010880800826940863	0.0	0.0	35.2665407602571	0.0
25	0.0010880800826940863	0.0	0.0	36.01964761749322	0.0
26	0.0010880800826940863	0.0	0.0	36.7433763124966	0.0
27	0.0010880800826940863	0.0	0.0	37.48824484910661	0.0
28	0.0012435200945075271	0.0	0.0	38.24896826692159	0.0
29	0.0012435200945075271	0.0	0.0	39.05026152781988	0.0
30	0.0012435200945075271	0.0	0.0	39.92212455408146	0.0
31	0.0013989601063209682	0.0	0.0	40.678029331530226	0.0
32	0.0013989601063209682	0.0	0.0	41.38885650555309	0.0
33	0.0013989601063209682	0.0	0.0	42.07030551734322	0.0
34	0.001554400118134409	0.0	0.0	42.760925489830335	0.0
35	0.001554400118134409	0.0	0.0	43.48667490498729	0.0
36	0.001554400118134409	0.0	0.0	44.15739855596229	0.0
37	0.0017098401299478498	0.0	0.0	44.79408084435014	0.0
38	0.0017098401299478498	0.0	0.0	45.462628335159756	0.0
39	0.0017098401299478498	0.0	0.0	46.21868855262033	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTACG	35	1.2108103E-7	45.000004	1
AGTACGG	60	0.0	45.000004	2
TCCGGCC	20	7.0309686E-4	45.0	41
CTATGCG	20	7.0309686E-4	45.0	1
TAATACG	25	3.8888837E-5	45.0	4
GGTACCG	20	7.0309686E-4	45.0	9
ACACGAC	20	7.0309686E-4	45.0	26
GATCGCG	25	3.8888837E-5	45.0	1
GGTCGAC	20	7.0309686E-4	45.0	8
GACGTAC	20	7.0309686E-4	45.0	10
CGTCTAG	20	7.0309686E-4	45.0	23
TCTAGCG	20	7.0309686E-4	45.0	1
GTCAGGT	20	7.0309686E-4	45.0	3
CTAACGG	20	7.0309686E-4	45.0	2
CCCCCTA	20	7.0309686E-4	45.0	21
TTGCGGT	20	7.0309686E-4	45.0	3
TACTACG	80	0.0	45.0	1
CCGGTCG	20	7.0309686E-4	45.0	23
TATCGCG	20	7.0309686E-4	45.0	1
AGTACGT	20	7.0309686E-4	45.0	19
>>END_MODULE