##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936471.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	542551
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12425928622378	33.0	31.0	34.0	30.0	34.0
2	32.142685203787295	33.0	31.0	34.0	30.0	34.0
3	32.17972503967369	34.0	31.0	34.0	30.0	34.0
4	35.81666792614888	37.0	35.0	37.0	35.0	37.0
5	34.867417072312094	37.0	35.0	37.0	33.0	37.0
6	35.35893031254205	37.0	35.0	37.0	33.0	37.0
7	35.90968406656702	37.0	35.0	37.0	35.0	37.0
8	35.921870939321835	37.0	35.0	37.0	35.0	37.0
9	37.775737211801285	39.0	38.0	39.0	35.0	39.0
10	37.43002777619063	39.0	37.0	39.0	35.0	39.0
11	37.347217127975064	39.0	37.0	39.0	35.0	39.0
12	37.18234230514735	39.0	37.0	39.0	34.0	39.0
13	37.03619383246921	39.0	37.0	39.0	33.0	39.0
14	38.077670117647926	40.0	37.0	41.0	33.0	41.0
15	38.227117819338645	40.0	37.0	41.0	33.0	41.0
16	38.29999944705659	40.0	37.0	41.0	34.0	41.0
17	38.30465707371289	40.0	37.0	41.0	34.0	41.0
18	38.27507091499233	40.0	37.0	41.0	34.0	41.0
19	38.22575204911612	40.0	37.0	41.0	34.0	41.0
20	38.22679158272678	40.0	37.0	41.0	34.0	41.0
21	38.1086810272214	40.0	36.0	41.0	34.0	41.0
22	38.14596968764227	40.0	36.0	41.0	34.0	41.0
23	38.15635765117012	40.0	36.0	41.0	34.0	41.0
24	38.133121125940235	40.0	36.0	41.0	34.0	41.0
25	37.961710512007166	40.0	36.0	41.0	34.0	41.0
26	38.00749054005983	40.0	36.0	41.0	34.0	41.0
27	38.02057871057283	40.0	36.0	41.0	34.0	41.0
28	37.88517945778369	40.0	36.0	41.0	34.0	41.0
29	37.850332964090015	40.0	36.0	41.0	34.0	41.0
30	37.73186483851288	40.0	36.0	41.0	33.0	41.0
31	37.70409602046628	40.0	36.0	41.0	33.0	41.0
32	37.67217459741112	40.0	36.0	41.0	33.0	41.0
33	37.546732012290086	40.0	35.0	41.0	33.0	41.0
34	37.43005910965052	40.0	35.0	41.0	33.0	41.0
35	37.36742168017385	40.0	35.0	41.0	33.0	41.0
36	37.29331436123056	40.0	35.0	41.0	33.0	41.0
37	37.22232564311926	40.0	35.0	41.0	33.0	41.0
38	37.146784357599564	39.0	35.0	41.0	32.0	41.0
39	37.08278668733446	39.0	35.0	41.0	32.0	41.0
40	36.93800951431294	39.0	35.0	41.0	32.0	41.0
41	36.85916715663597	39.0	35.0	41.0	31.0	41.0
42	36.86848609623796	39.0	35.0	41.0	32.0	41.0
43	36.74269147047927	39.0	35.0	41.0	31.0	41.0
44	36.60925516679538	39.0	35.0	41.0	31.0	41.0
45	36.6160803316186	39.0	35.0	41.0	31.0	41.0
46	36.52100539857083	39.0	35.0	41.0	31.0	41.0
47	36.450175190903714	39.0	35.0	41.0	31.0	41.0
48	36.41598301357845	38.0	35.0	41.0	31.0	41.0
49	36.35102506492477	38.0	35.0	40.0	31.0	41.0
50	36.19987429753148	38.0	35.0	40.0	31.0	41.0
51	35.08063020803574	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	12.0
10	18.0
11	12.0
12	11.0
13	11.0
14	21.0
15	30.0
16	29.0
17	57.0
18	135.0
19	214.0
20	391.0
21	674.0
22	1030.0
23	1395.0
24	2030.0
25	2684.0
26	3575.0
27	4374.0
28	4826.0
29	5967.0
30	7463.0
31	9491.0
32	12821.0
33	18764.0
34	35520.0
35	47036.0
36	38451.0
37	59417.0
38	103837.0
39	182095.0
40	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.13677792502456	22.194042587701432	25.452538102408806	11.216641384865202
2	31.600531562931412	24.937747787765574	25.754445204229647	17.707275445073368
3	31.824657958422343	24.4032358248349	26.452628416499095	17.319477800243664
4	28.81996346887205	25.38876529579708	26.247117782475748	19.544153452855124
5	25.46267539825749	31.25254584361655	24.763201984698213	18.52157677342775
6	26.216521580459716	33.785395290028035	24.85757099332597	15.140512136186276
7	79.06574681458517	6.713838883349216	9.07509155821296	5.145322743852652
8	80.53528608370458	5.839635352252599	8.457085140383116	5.167993423659711
9	74.23762927356138	7.635042604289735	10.403077314390721	7.724250807758165
10	42.17907625273937	23.57547954017226	18.36122318454855	15.884221022539815
11	32.34239730458519	26.008061914916752	23.586907037310777	18.062633743187277
12	28.82383407274155	22.588475553450273	29.160576609387874	19.427113764420305
13	25.520550141829982	24.654640761882295	30.16398458393773	19.660824512349993
14	20.373199938807595	29.111548960374233	29.389310866628204	21.125940234189965
15	18.530423867986602	25.747994197780482	35.80142696262655	19.920154971606355
16	22.88927676845126	24.674362410169735	31.38764835010902	21.04871247126998
17	24.009724431435938	24.190536926482487	27.280569015631713	24.519169626449862
18	25.534189412608217	23.929547636996336	29.669653175461846	20.8666097749336
19	25.15358003210758	26.02888945002405	26.8997753206611	21.917755197207267
20	26.040685576102522	26.71122161787555	27.355032061502055	19.893060744519868
21	24.68560559283828	26.815912236821976	28.870281319175522	19.628200851164223
22	23.735648814581488	24.664040799851076	28.01672100871623	23.583589376851208
23	21.54617722573546	26.839135860038965	28.663664798332324	22.951022115893252
24	22.777213570705797	24.957469436053017	29.27227117819339	22.993045815047804
25	22.263529142882422	27.019764040615534	27.390604754207438	23.326102062294606
26	20.84449203853647	27.739880674812138	27.53676612889848	23.87886115775291
27	21.363337271519175	27.384706691168205	29.125741174562393	22.126214862750228
28	19.84624486914594	27.296420060049652	30.185549376924936	22.67178569387947
29	21.626169705705088	26.838398602159057	28.697025717397995	22.838405974737856
30	21.782099747304862	27.120399741222485	28.705504183016895	22.391996328455757
31	23.39300821489593	28.40894220082536	26.266102172883286	21.931947411395427
32	25.76624133030812	28.2552239328653	25.51280893409099	20.465725802735594
33	24.292462828379268	26.475667725246105	26.504605097032353	22.727264349342274
34	22.159944410755855	27.733429668362973	27.835171255789774	22.27145466509139
35	22.645981668082815	27.556856406125874	27.544138707697524	22.253023218093784
36	24.060410910679366	27.47981295767587	27.331992752755042	21.127783378889724
37	22.593636358609608	28.678225641460436	27.991285611859528	20.73685238807043
38	23.907982844009133	27.99792093277867	24.96539495826199	23.12870126495021
39	24.274031381381658	26.085289677836737	26.646711553383923	22.99396738739768
40	24.92945363661665	25.442032177620167	27.275223896002405	22.35329028976078
41	21.971943651380236	25.938575359735765	27.174588195395454	24.914892793488537
42	23.806978514462234	25.37014953432949	27.964191384773045	22.85868056643523
43	23.377157170477982	25.143995679668823	28.332636010255257	23.146211139597934
44	22.219477984558132	26.177446912824788	27.880328300933922	23.722746801683158
45	21.964571072581197	25.595381816640277	26.76854341803812	25.671503692740405
46	23.09220699989494	26.78937095314542	26.77038656273788	23.34803548422176
47	21.342509736411877	26.67177832130067	28.96077972393379	23.024932218353666
48	22.173583681534087	26.022069814634936	27.99405032890917	23.810296174921806
49	23.85729636476571	23.897845548160447	29.419907068644235	22.824951018429605
50	21.7632996713673	25.24334117898594	29.231537680328668	23.761821469318093
51	21.308595873936277	25.76624133030812	26.00640308468697	26.918759711068635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	191.0
1	361.5
2	532.0
3	1174.5
4	1817.0
5	1313.0
6	809.0
7	797.5
8	786.0
9	806.5
10	827.0
11	870.0
12	913.0
13	852.5
14	792.0
15	813.5
16	835.0
17	971.0
18	1107.0
19	1098.0
20	1089.0
21	1434.0
22	1779.0
23	1729.5
24	1680.0
25	2152.5
26	3411.0
27	4197.0
28	5613.0
29	7029.0
30	7666.5
31	8304.0
32	9616.5
33	10929.0
34	12116.0
35	13303.0
36	15174.0
37	17045.0
38	19074.0
39	21103.0
40	23369.0
41	25635.0
42	29200.0
43	32765.0
44	36533.0
45	40301.0
46	45231.0
47	50161.0
48	52737.0
49	55313.0
50	52736.5
51	50160.0
52	44558.5
53	38957.0
54	35519.5
55	32082.0
56	29616.5
57	27151.0
58	25333.0
59	23515.0
60	22163.0
61	20811.0
62	18804.5
63	16798.0
64	13602.5
65	10407.0
66	9069.0
67	7731.0
68	6406.0
69	5081.0
70	4215.5
71	3350.0
72	2740.5
73	2131.0
74	1607.0
75	947.5
76	812.0
77	580.5
78	349.0
79	245.5
80	142.0
81	114.5
82	87.0
83	51.0
84	15.0
85	9.5
86	4.0
87	3.5
88	3.0
89	4.5
90	6.0
91	4.0
92	2.0
93	3.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	542551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.313463250061275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75368982988286	23.407969192945163
2	10.03283432480911	6.283255778477267
3	3.4294361359437984	3.2216256723392482
4	1.694497088452435	2.1224228922636463
5	1.0574503966029654	1.6556217066394838
6	0.7080173601973636	1.3302285353367325
7	0.5515618693064861	1.2089918627264626
8	0.4603641719062898	1.1532477262905996
9	0.3884371815829245	1.0946982069408857
>10	6.578595105288211	49.48524189063638
>50	0.30795013983999087	5.767318079956611
>100	0.03244685381455842	1.793927798669399
>500	0.00353965677977001	0.9811091070675706
>1k	0.0011798855932566698	0.49434154971056654
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	1488	0.2742599313244285	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	1188	0.21896559033160015	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC	992	0.18283995421628566	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	970	0.17878503587681158	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC	959	0.17675757670707454	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG	904	0.16662028085838934	No Hit
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT	884	0.16293399145886747	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	602	0.11095731092560883	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.843144699760944E-4	0.0	0.0	0.17915366481676376	0.0
2	1.843144699760944E-4	0.0	0.0	0.9317096457291573	0.0
3	1.843144699760944E-4	0.0	0.0	1.2990483843915135	0.0
4	1.843144699760944E-4	0.0	0.0	1.766654194720865	0.0
5	1.843144699760944E-4	0.0	0.0	3.049482905754482	0.0
6	1.843144699760944E-4	0.0	0.0	3.7981682827973775	0.0
7	1.843144699760944E-4	0.0	0.0	4.489347545207732	0.0
8	3.686289399521888E-4	0.0	0.0	5.606108918792888	0.0
9	3.686289399521888E-4	0.0	0.0	6.024502765638622	0.0
10	3.686289399521888E-4	0.0	0.0	7.072883470862647	0.0
11	3.686289399521888E-4	0.0	0.0	8.280143249206066	0.0
12	3.686289399521888E-4	0.0	0.0	9.40685760416993	0.0
13	5.529434099282832E-4	0.0	0.0	9.920357717523329	0.0
14	5.529434099282832E-4	0.0	0.0	10.181715635949432	0.0
15	5.529434099282832E-4	0.0	0.0	10.54094453793284	0.0
16	5.529434099282832E-4	0.0	0.0	11.190653044598573	0.0
17	7.372578799043776E-4	0.0	0.0	11.955926723939315	0.0
18	7.372578799043776E-4	0.0	0.0	12.883581451329	0.0
19	7.372578799043776E-4	0.0	0.0	13.411458093340533	0.0
20	7.372578799043776E-4	0.0	0.0	13.913715024025391	0.0
21	9.215723498804721E-4	0.0	0.0	14.533564586554997	0.0
22	9.215723498804721E-4	0.0	0.0	15.151755318854818	0.0
23	9.215723498804721E-4	0.0	0.0	15.754095006736694	0.0
24	9.215723498804721E-4	0.0	0.0	16.24603032710289	0.0
25	0.0011058868198565665	0.0	0.0	16.670506551457834	0.0
26	0.0011058868198565665	0.0	0.0	17.0940612034629	0.0
27	0.0011058868198565665	0.0	0.0	17.545447340434354	0.0
28	0.0011058868198565665	0.0	0.0	17.986696181557125	0.0
29	0.0011058868198565665	0.0	0.0	18.42536462010023	0.0
30	0.0011058868198565665	0.0	0.0	18.96337855796045	0.0
31	0.0011058868198565665	0.0	0.0	19.434117714279395	0.0
32	0.0011058868198565665	0.0	0.0	19.862095913563888	0.0
33	0.0011058868198565665	0.0	0.0	20.332650755412857	0.0
34	0.0011058868198565665	0.0	0.0	20.80375854067175	0.0
35	0.0011058868198565665	0.0	0.0	21.3084115594663	0.0
36	0.0011058868198565665	0.0	0.0	21.774358539565867	0.0
37	0.0011058868198565665	0.0	0.0	22.21265834916902	0.0
38	0.0011058868198565665	0.0	0.0	22.700907380135693	0.0
39	0.0011058868198565665	0.0	0.0	23.333290326623672	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAACTA	20	7.029992E-4	45.0	10
TAGCGAG	45	3.8380676E-10	45.0	1
TTGTACG	35	1.2103374E-7	45.0	1
ATCCGGT	20	7.029992E-4	45.0	44
CGTAAGG	35	1.2103374E-7	45.0	2
CGGTCTA	40	6.8030204E-9	45.0	31
ATCGGCT	20	7.029992E-4	45.0	13
ACGACTT	20	7.029992E-4	45.0	14
TATCCGG	20	7.029992E-4	45.0	2
CTCGTCC	55	6.002665E-11	40.909092	37
GTTTGCG	45	1.9250365E-8	40.0	1
CGTATGG	45	1.9250365E-8	40.0	2
CGAATGG	45	1.9250365E-8	40.0	2
ACGGTCT	45	1.9250365E-8	40.0	30
TCGATAG	40	3.4537152E-7	39.375	1
GCTACGA	40	3.4537152E-7	39.375	10
ACGGGTA	35	6.242193E-6	38.571426	5
TTACGCG	35	6.242193E-6	38.571426	1
GCCGTTG	70	0.0	38.571426	14
TGCCGTT	70	0.0	38.571426	13
>>END_MODULE