SRR2936470.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936470.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	627917
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC	12719	2.0255861841612823	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC	11303	1.8000786728182228	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG	10959	1.7452943621529597	No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	5760	0.9173186902090563	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	2903	0.4623222495966824	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	2457	0.3912937537923006	No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	1982	0.31564681319346344	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	1780	0.2834769563493264	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTA	1550	0.24684791142778423	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC	1547	0.2463701412766337	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTT	1517	0.2415924397651282	No Hit
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1370	0.21818170235875126	TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1023	0.1629196215423376	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	996	0.15861969018198266	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCC	672	0.10702051385772324	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGG	35	1.2107557E-7	45.000004	2
GCCGGAT	25	3.888776E-5	45.000004	19
TCGATTG	25	3.888776E-5	45.000004	1
TACGGGT	25	3.888776E-5	45.000004	4
CGTATCG	20	7.0308393E-4	45.0	15
TCGGAGT	20	7.0308393E-4	45.0	4
CGGATAA	20	7.0308393E-4	45.0	21
TGTTACG	20	7.0308393E-4	45.0	1
AATTCGG	20	7.0308393E-4	45.0	2
CTCGCAC	20	7.0308393E-4	45.0	41
ATACGAG	20	7.0308393E-4	45.0	1
GCGTCGA	20	7.0308393E-4	45.0	29
TATCGCG	20	7.0308393E-4	45.0	1
CTGGCGT	20	7.0308393E-4	45.0	14
GCGTAAG	40	6.8066583E-9	45.0	1
AGACCGG	30	2.1639225E-6	44.999996	2
CGTTTTT	660	0.0	41.25	1
TTTACGG	55	6.002665E-11	40.909092	2
TACGGCT	1595	0.0	40.203762	7
ATAGGCG	40	3.4548793E-7	39.375	12