##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936470.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	627917
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.794256247242867	33.0	31.0	34.0	30.0	34.0
2	31.850048652927057	33.0	31.0	34.0	30.0	34.0
3	31.903089102540623	33.0	31.0	34.0	30.0	34.0
4	35.61559409922012	37.0	35.0	37.0	33.0	37.0
5	34.66333448529026	37.0	35.0	37.0	33.0	37.0
6	35.13300324724446	37.0	35.0	37.0	32.0	37.0
7	35.700506595616936	37.0	35.0	37.0	33.0	37.0
8	35.63441824317545	37.0	35.0	37.0	33.0	37.0
9	37.501098075064064	39.0	37.0	39.0	35.0	39.0
10	37.17961291062354	39.0	37.0	39.0	34.0	39.0
11	37.10339264584332	39.0	37.0	39.0	34.0	39.0
12	37.03387868141809	39.0	37.0	39.0	33.0	39.0
13	36.8952887085395	39.0	37.0	39.0	33.0	39.0
14	37.93246081886619	40.0	37.0	41.0	33.0	41.0
15	37.9717908577089	40.0	37.0	41.0	33.0	41.0
16	38.09809098973272	40.0	37.0	41.0	33.0	41.0
17	38.005545318887684	40.0	37.0	41.0	33.0	41.0
18	38.022974374001656	40.0	37.0	41.0	33.0	41.0
19	37.978681895855665	40.0	37.0	41.0	33.0	41.0
20	37.89909653664417	40.0	36.0	41.0	33.0	41.0
21	37.91793501370404	40.0	36.0	41.0	33.0	41.0
22	37.95210991261584	40.0	37.0	41.0	33.0	41.0
23	37.95052530828119	40.0	36.0	41.0	34.0	41.0
24	37.86035893278889	40.0	36.0	41.0	33.0	41.0
25	37.77953455631875	39.0	36.0	41.0	33.0	41.0
26	37.79238020311602	40.0	36.0	41.0	33.0	41.0
27	37.777656919624725	40.0	36.0	41.0	33.0	41.0
28	37.69812889283138	40.0	36.0	41.0	33.0	41.0
29	37.66100455951981	40.0	36.0	41.0	33.0	41.0
30	37.59377911411858	40.0	36.0	41.0	33.0	41.0
31	37.60561188819541	40.0	36.0	41.0	33.0	41.0
32	37.40778319427567	40.0	36.0	41.0	33.0	41.0
33	37.36273583929086	40.0	36.0	41.0	33.0	41.0
34	37.307088357219186	40.0	36.0	41.0	32.0	41.0
35	37.207693054973824	40.0	35.0	41.0	32.0	41.0
36	37.19049810723392	40.0	35.0	41.0	32.0	41.0
37	37.14591259672855	39.0	35.0	41.0	32.0	41.0
38	37.046767327528954	39.0	35.0	41.0	31.0	41.0
39	36.994234906842784	39.0	35.0	41.0	31.0	41.0
40	36.813491273528186	39.0	35.0	41.0	31.0	41.0
41	36.73293922604421	39.0	35.0	41.0	31.0	41.0
42	36.79767708152511	39.0	35.0	41.0	31.0	41.0
43	36.75673695727301	39.0	35.0	41.0	31.0	41.0
44	36.691573886357595	39.0	35.0	41.0	31.0	41.0
45	36.668505550892874	39.0	35.0	41.0	31.0	41.0
46	36.5185287227452	39.0	35.0	40.0	31.0	41.0
47	36.33284335350054	39.0	35.0	40.0	30.0	41.0
48	36.3143488709495	39.0	35.0	40.0	30.0	41.0
49	36.347219457348665	39.0	35.0	40.0	30.0	41.0
50	36.19447315489149	38.0	35.0	40.0	30.0	41.0
51	35.022649490298875	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	8.0
11	10.0
12	7.0
13	9.0
14	9.0
15	16.0
16	26.0
17	51.0
18	117.0
19	252.0
20	482.0
21	872.0
22	1529.0
23	2053.0
24	2655.0
25	3344.0
26	4357.0
27	5132.0
28	6051.0
29	7573.0
30	9792.0
31	12915.0
32	17469.0
33	23776.0
34	38719.0
35	51883.0
36	49255.0
37	73063.0
38	127808.0
39	188600.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.00483344136247	20.725987670344967	23.905388769534827	10.363790118757734
2	27.85224798818952	30.744827739972003	24.614877443993393	16.788046827845083
3	27.58055602890191	30.920965669029503	26.068254243793366	15.430224058275218
4	26.508280553003022	23.128693760481084	32.70878157463486	17.654244111881027
5	28.582758549298713	27.91324331081974	24.104618922564605	19.39937921731694
6	22.55091039102302	36.923988361519115	25.385361441082182	15.139739806375681
7	72.01509116650767	5.6985238494896615	16.472081501217517	5.814303482785145
8	70.76619999140014	10.455362731061589	11.406762358719384	7.371674918818888
9	65.50706542425193	6.579372751494226	12.909508740804915	15.004053083448927
10	40.084597168097055	22.839961332469098	20.67072558952855	16.404715909905292
11	28.762400126131322	23.03441378398737	27.12810769576234	21.075078394118968
12	24.482853625558793	19.9752515061704	32.164760629191434	23.37713423907937
13	22.235900604697754	23.365030728583555	36.89197776139203	17.50709090532666
14	18.89835758547706	29.3519684926511	29.282532564017217	22.46714135785462
15	15.25758977699282	24.248905508212072	40.30102704656826	20.192477668226854
16	17.677336335853305	25.995473924101436	30.049353656613853	26.27783608343141
17	17.633540738664504	25.966807715032402	30.55117157203898	25.848479974264116
18	18.544170646757454	25.257159783856785	32.40810489284412	23.790564676541646
19	19.500029462492655	27.242454018604366	28.3539066468976	24.90360987200538
20	20.552238592043214	27.531186446616353	32.03592194509784	19.88065301624259
21	20.872981620182284	30.31053467257615	29.162134485927282	19.654349221314284
22	18.518211801878273	24.096496829995047	29.6144235623498	27.770867805776877
23	18.009068077468836	27.95433154381869	30.048398116311553	23.98820226240092
24	21.448694652318697	23.58942344290726	28.72784141853143	26.23404048624261
25	17.397203770562033	30.97590923641182	26.613230729539094	25.013656263487054
26	17.30021642987847	25.187723855222906	29.376812540510926	28.135247174387697
27	21.02220516405831	26.559083445742033	25.993562843496832	26.425148546702832
28	15.27574504273654	24.75088268035425	31.735723033458246	28.237649243450964
29	18.399406290958837	22.684049006476968	27.704139241332854	31.21240546123134
30	18.528563488486537	25.3533508409551	29.12805354847854	26.99003212207983
31	22.002908027653337	24.232661323072954	23.244314137059515	30.520116512214194
32	20.856896691760216	28.59916199115488	25.42103494570142	25.12290637138348
33	19.432982384614526	22.426849408440923	28.63595029279347	29.504217914151077
34	22.002908027653337	21.82103685678203	28.126488054949938	28.0495670606147
35	17.340189865858065	22.537692083507853	30.424880995418185	29.697237055215897
36	19.42740839951777	21.715608910094804	30.686539781531636	28.170442908855787
37	18.770474441685764	24.69705390999129	31.07082623977373	25.46164540854922
38	17.110700936588753	25.1819906134091	30.162107412285383	27.545201037716765
39	22.827539308539187	24.388892162499186	30.816334005927537	21.967234523034097
40	21.098170618091245	20.482802663409334	34.96194560746086	23.457081111038562
41	20.29408345370487	22.489755811675746	29.977369620507172	27.238791114112214
42	18.712504996679495	20.609571010181284	34.39674351864976	26.281180474489464
43	21.278449221792055	20.984779835551514	30.75072023850286	26.986050704153573
44	20.145656193414098	21.488349574864195	29.731317992664636	28.63467623905707
45	19.492862910225394	20.934454712963657	28.585306656771515	30.987375720039434
46	25.61851327484365	22.519696074481182	27.925346821315557	23.936443829359614
47	17.038716900482072	20.741276315181782	37.071619338224636	25.14838744611151
48	19.453526501114002	21.79030031039134	30.279638869468418	28.476534319026243
49	19.38918678742573	18.35943285497924	36.748965229480966	25.502415128114066
50	20.801953124377903	18.739897152012126	32.802424524260374	27.655725199349597
51	19.399060703882835	18.56805915431498	28.20579790004093	33.827082241761254
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	193.0
1	298.5
2	404.0
3	1113.5
4	1823.0
5	1276.5
6	730.0
7	726.0
8	722.0
9	781.5
10	841.0
11	848.5
12	856.0
13	888.0
14	920.0
15	962.0
16	1004.0
17	967.0
18	930.0
19	971.0
20	1012.0
21	1317.0
22	1622.0
23	1868.0
24	2114.0
25	2350.5
26	3284.5
27	3982.0
28	4776.0
29	5570.0
30	6624.5
31	7679.0
32	8850.5
33	10022.0
34	11219.0
35	12416.0
36	14081.0
37	15746.0
38	17772.0
39	19798.0
40	22562.5
41	25327.0
42	29389.0
43	33451.0
44	39116.5
45	44782.0
46	75685.5
47	106589.0
48	94961.5
49	83334.0
50	79036.5
51	74739.0
52	60487.5
53	46236.0
54	38714.0
55	31192.0
56	27901.5
57	24611.0
58	21965.0
59	19319.0
60	16952.0
61	14585.0
62	13066.0
63	11547.0
64	9655.5
65	7764.0
66	6467.5
67	5171.0
68	4285.5
69	3400.0
70	2580.0
71	1760.0
72	1594.0
73	1428.0
74	1183.0
75	673.0
76	408.0
77	286.5
78	165.0
79	130.5
80	96.0
81	71.5
82	47.0
83	39.5
84	32.0
85	18.5
86	5.0
87	4.0
88	3.0
89	6.0
90	9.0
91	8.5
92	8.0
93	4.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	627917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.38686970616815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.85797180230833	24.32499611619341
2	11.837310956029915	7.904215171207351
3	4.373250115341926	4.380273953802172
4	2.1392367443043288	2.8568967381094392
5	1.2744282904732438	2.12745856419424
6	0.8575720670456519	1.717898811966149
7	0.6274602386797618	1.4664253264221663
8	0.48522792858151614	1.2960193303475955
9	0.37673377635980454	1.1320165354713736
>10	4.970733858176266	36.812812848600146
>50	0.13832882861314968	2.8913531656331632
>100	0.051693818304651466	3.1641404233115025
>500	0.003829171726270479	0.8373785766743571
>1k	0.004307818192054289	2.577496004423799
>5k	4.7864646578380985E-4	0.9204772140542552
>10k+	0.0014359393973514295	5.590141219588872
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC	12719	2.0255861841612823	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC	11303	1.8000786728182228	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG	10959	1.7452943621529597	No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	5760	0.9173186902090563	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	2903	0.4623222495966824	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	2457	0.3912937537923006	No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	1982	0.31564681319346344	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	1780	0.2834769563493264	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTA	1550	0.24684791142778423	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC	1547	0.2463701412766337	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTT	1517	0.2415924397651282	No Hit
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1370	0.21818170235875126	TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1023	0.1629196215423376	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	996	0.15861969018198266	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCC	672	0.10702051385772324	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.370268682007335E-4	0.0	0.0	0.3353946461076862	0.0
2	6.370268682007335E-4	0.0	0.0	2.2209941759818577	0.0
3	6.370268682007335E-4	0.0	0.0	2.950071426637597	0.0
4	6.370268682007335E-4	0.0	0.0	4.084138508751953	0.0
5	6.370268682007335E-4	0.0	0.0	8.132762769601715	0.0
6	6.370268682007335E-4	0.0	0.0	9.554925252859853	0.0
7	6.370268682007335E-4	0.0	0.0	11.284453200024844	0.0
8	6.370268682007335E-4	0.0	0.0	13.644797003425612	0.0
9	6.370268682007335E-4	0.0	0.0	14.425154916971511	0.0
10	6.370268682007335E-4	0.0	0.0	17.92673235475389	0.0
11	6.370268682007335E-4	0.0	0.0	20.186107399544845	0.0
12	6.370268682007335E-4	0.0	0.0	24.09028581803009	0.0
13	6.370268682007335E-4	0.0	0.0	24.92415398850485	0.0
14	6.370268682007335E-4	0.0	0.0	25.277226130205108	0.0
15	6.370268682007335E-4	0.0	0.0	26.043091682499437	0.0
16	6.370268682007335E-4	0.0	0.0	27.166966334722584	0.0
17	6.370268682007335E-4	0.0	0.0	28.521126199800293	0.0
18	6.370268682007335E-4	0.0	0.0	29.968769757786458	0.0
19	7.962835852509169E-4	0.0	0.0	31.101403529447364	0.0
20	9.555403023011003E-4	0.0	0.0	31.946101156681536	0.0
21	9.555403023011003E-4	0.0	0.0	32.93014841133462	0.0
22	0.0011147970193512836	0.0	0.0	34.040964012759645	0.0
23	0.0011147970193512836	0.0	0.0	35.10989509760048	0.0
24	0.0011147970193512836	0.0	0.0	35.9135044918357	0.0
25	0.0011147970193512836	0.0	0.0	36.61614512746112	0.0
26	0.0011147970193512836	0.0	0.0	37.30556745557136	0.0
27	0.0011147970193512836	0.0	0.0	38.01808200765388	0.0
28	0.0011147970193512836	0.0	0.0	38.6877565028499	0.0
29	0.0011147970193512836	0.0	0.0	39.372241872731585	0.0
30	0.0011147970193512836	0.0	0.0	40.13444452053376	0.0
31	0.0011147970193512836	0.0	0.0	40.81972617400071	0.0
32	0.001274053736401467	0.0	0.0	41.42840534656651	0.0
33	0.0014333104534516505	0.0	0.0	42.01685891606693	0.0
34	0.0014333104534516505	0.0	0.0	42.62553808863273	0.0
35	0.0015925671705018338	0.0	0.0	43.26638711804267	0.0
36	0.0015925671705018338	0.0	0.0	43.849585215880445	0.0
37	0.0015925671705018338	0.0	0.0	44.40236528076163	0.005573985096756418
38	0.0015925671705018338	0.0	0.0	44.95132318443361	0.005573985096756418
39	0.0015925671705018338	0.0	0.0	45.534521282271385	0.005573985096756418
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGG	35	1.2107557E-7	45.000004	2
GCCGGAT	25	3.888776E-5	45.000004	19
TCGATTG	25	3.888776E-5	45.000004	1
TACGGGT	25	3.888776E-5	45.000004	4
CGTATCG	20	7.0308393E-4	45.0	15
TCGGAGT	20	7.0308393E-4	45.0	4
CGGATAA	20	7.0308393E-4	45.0	21
TGTTACG	20	7.0308393E-4	45.0	1
AATTCGG	20	7.0308393E-4	45.0	2
CTCGCAC	20	7.0308393E-4	45.0	41
ATACGAG	20	7.0308393E-4	45.0	1
GCGTCGA	20	7.0308393E-4	45.0	29
TATCGCG	20	7.0308393E-4	45.0	1
CTGGCGT	20	7.0308393E-4	45.0	14
GCGTAAG	40	6.8066583E-9	45.0	1
AGACCGG	30	2.1639225E-6	44.999996	2
CGTTTTT	660	0.0	41.25	1
TTTACGG	55	6.002665E-11	40.909092	2
TACGGCT	1595	0.0	40.203762	7
ATAGGCG	40	3.4548793E-7	39.375	12
>>END_MODULE