FastQCFastQC Report
Sat 14 Jan 2017
SRR2936469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069187
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC128141.1984807147860943No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC118821.1113116788737611No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG118681.1100022727549064No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC58770.5496699828935444Illumina PCR Primer Index 3 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC48190.45071629191151785RNA PCR Primer, Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT30060.28114819951982206TruSeq Adapter, Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.2584206504568424No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT23470.21951258292515716No Hit
GAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT22110.20679263777056775TruSeq Adapter, Index 7 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGTCACCT20100.1879933070641525No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC18570.1736833687652394No Hit
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG17370.162459887746484TruSeq Adapter, Index 7 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTA16790.1570352052540856No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG15740.1472146593626746No Hit
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC13620.12738650956287345Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC12000.11223481018755373No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT207.0330664E-445.025
CGAATAT850.045.014
GCGTACA207.0330664E-445.02
GCTACGA900.042.510
CGTTTTT16150.041.238391
TACGGCT15050.040.0664447
GACGTAG1200.039.3750041
GGTACGA403.457917E-739.3758
GCGTTAG750.039.01
CGAGTAT356.247881E-638.57142627
TTGTACG700.038.5714261
CCGAGTA356.247881E-638.57142626
TACGGGA3100.038.467744
ACGGCTG15700.038.4076428
CTACGAA1000.038.25000411
ACGTAGG2250.038.02
TTAGACG301.13986855E-437.5000041
CGTCTTA301.13986855E-437.50000417
CGCTCAT301.13986855E-437.50000421
CGCTATC301.13986855E-437.50000442