##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936469.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1069187
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.993365052137747	33.0	31.0	34.0	30.0	34.0
2	32.05096676259625	33.0	31.0	34.0	30.0	34.0
3	32.07891323033295	33.0	31.0	34.0	30.0	34.0
4	35.747024608417426	37.0	35.0	37.0	35.0	37.0
5	34.83202283604271	37.0	35.0	37.0	33.0	37.0
6	35.30062842140804	37.0	35.0	37.0	33.0	37.0
7	35.85060050299901	37.0	35.0	37.0	35.0	37.0
8	35.79977777507583	37.0	35.0	37.0	35.0	37.0
9	37.656774726965445	39.0	37.0	39.0	35.0	39.0
10	37.37592675556287	39.0	37.0	39.0	34.0	39.0
11	37.27111066632872	39.0	37.0	39.0	34.0	39.0
12	37.115643942547	39.0	37.0	39.0	34.0	39.0
13	36.96003224880213	39.0	37.0	39.0	33.0	39.0
14	37.86942695711789	40.0	37.0	41.0	33.0	41.0
15	37.98854269646002	40.0	37.0	41.0	33.0	41.0
16	38.135053082388765	40.0	37.0	41.0	33.0	41.0
17	38.111386502080556	40.0	37.0	41.0	33.0	41.0
18	38.113829479782304	40.0	37.0	41.0	34.0	41.0
19	38.07467168979795	40.0	37.0	41.0	34.0	41.0
20	37.996780731527785	40.0	36.0	41.0	33.0	41.0
21	37.99312281200576	40.0	36.0	41.0	33.0	41.0
22	38.050613222944165	40.0	36.0	41.0	34.0	41.0
23	38.062319313646725	40.0	36.0	41.0	34.0	41.0
24	38.01294254419479	40.0	36.0	41.0	34.0	41.0
25	37.90094997413923	40.0	36.0	41.0	34.0	41.0
26	37.90704245375225	40.0	36.0	41.0	34.0	41.0
27	37.88857608631605	40.0	36.0	41.0	34.0	41.0
28	37.80175404302521	40.0	36.0	41.0	33.0	41.0
29	37.75572280620696	40.0	36.0	41.0	33.0	41.0
30	37.63990489970417	40.0	36.0	41.0	33.0	41.0
31	37.57231896758939	40.0	36.0	41.0	33.0	41.0
32	37.504474895411185	40.0	35.0	41.0	33.0	41.0
33	37.37338089595178	40.0	35.0	41.0	33.0	41.0
34	37.26751073479195	40.0	35.0	41.0	32.0	41.0
35	37.21337801525832	40.0	35.0	41.0	32.0	41.0
36	37.16222793580543	40.0	35.0	41.0	32.0	41.0
37	37.0894436613988	39.0	35.0	41.0	32.0	41.0
38	37.03694957009391	39.0	35.0	41.0	32.0	41.0
39	36.95866111353767	39.0	35.0	41.0	32.0	41.0
40	36.83664878080261	39.0	35.0	41.0	31.0	41.0
41	36.690759427490235	39.0	35.0	41.0	31.0	41.0
42	36.80671201576525	39.0	35.0	41.0	31.0	41.0
43	36.73160915723816	39.0	35.0	41.0	31.0	41.0
44	36.62579791935368	39.0	35.0	41.0	31.0	41.0
45	36.60831828295705	39.0	35.0	41.0	31.0	41.0
46	36.515303684014114	39.0	35.0	41.0	31.0	41.0
47	36.369816505438244	39.0	35.0	41.0	31.0	41.0
48	36.35227046344559	39.0	35.0	40.0	31.0	41.0
49	36.379464022663946	39.0	35.0	40.0	31.0	41.0
50	36.268235584607744	39.0	35.0	40.0	31.0	41.0
51	35.04853968482595	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	11.0
10	15.0
11	14.0
12	17.0
13	21.0
14	20.0
15	33.0
16	68.0
17	118.0
18	221.0
19	431.0
20	871.0
21	1640.0
22	2396.0
23	3397.0
24	4540.0
25	6054.0
26	7549.0
27	8893.0
28	10322.0
29	12148.0
30	15431.0
31	20412.0
32	28041.0
33	40858.0
34	73252.0
35	83488.0
36	77108.0
37	115822.0
38	204705.0
39	351091.0
40	192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88577489251178	20.781678041352915	23.322019440939705	12.010527625195593
2	30.823420037841835	27.901293225600387	23.96456372926345	17.310723007294328
3	29.921332750959373	28.112575255778456	26.09141338231759	15.874678610944578
4	27.2888652780103	23.943332644336305	30.769547328951813	17.998254748701584
5	26.898568725583083	29.280752571813913	24.88311212164009	18.93756658096292
6	24.705687592535263	35.15110078966542	25.728240242352367	14.414971375446953
7	75.20658219750148	5.752875783188535	13.772520616131697	5.268021403178303
8	74.71527431590545	8.62655456903236	10.4089368838192	6.249234231242991
9	68.46248598233984	7.052461356151918	12.52905244826209	11.956000213246138
10	41.99115776753739	22.57958617154904	20.302622459869042	15.126633601044531
11	31.259171688395014	23.762447541917364	26.09178749835155	18.88659327133607
12	27.496312618840296	20.6474639141703	31.04929259334429	20.806930873645115
13	25.67399341742838	22.56181565993601	33.89715737284498	17.867033549790634
14	20.004545509812598	27.201976829123435	31.586242631083245	21.207235029980723
15	17.78313802917544	24.146758237801244	38.12420091153372	19.9459028214896
16	21.978755821011667	25.087473005189924	29.44480245270472	23.48896872109369
17	22.177692022069103	24.829987644817976	29.329107069203047	23.663213263909867
18	22.95940747502542	24.32895274633904	31.02553622518792	21.68610355344762
19	23.062663500397964	25.7802423710726	27.807015985042842	23.350078143486595
20	22.217535379685685	27.895868543107987	30.748783889067116	19.13781218813921
21	21.859693393204367	28.392601107196402	31.265718718989287	18.48198678060994
22	20.173833015178822	23.608685851960416	31.26253873270064	24.95494240016012
23	19.370886477295365	26.571404253886367	31.263567551794026	22.794141717024242
24	21.975949950756977	25.04575906740355	29.19844704434304	23.779843937496434
25	20.565251915707915	29.361000461098012	26.97488839651062	23.09885922668345
26	18.967121747645642	25.329339021144104	30.131118317001608	25.57242091420865
27	21.18534924199415	27.16942873416905	28.63418653612511	23.01103548771169
28	18.432977580161374	25.453826131443797	31.30415914147853	24.809037146916303
29	20.894567554599895	24.43192818468612	31.36813298328543	23.305371277428552
30	21.706586406306847	27.98556286224954	28.710786794078118	21.597063937365494
31	23.955584944448447	26.0219213290098	27.05130159644665	22.97119213009511
32	23.73298590424313	26.003215527311873	27.961619436076194	22.3021791323688
33	22.267760457244616	24.614777396283344	25.537254007016546	27.580208139455493
34	20.552999615595773	25.18857786336721	30.859335177101855	23.399087343935157
35	18.776603157352266	26.42157078228598	28.310482637742506	26.49134342261924
36	22.671712244911323	25.874613140638637	26.84357366859118	24.61010094585886
37	20.072073453942107	28.128101071187732	29.588556538753274	22.21126893611688
38	21.97220879041739	24.845419931218768	26.313918893514415	26.868452384849423
39	20.99688828988755	24.312865756878825	28.768681250333195	25.92156470290043
40	21.325549225720103	23.533769116160222	28.80235169338946	26.33832996473021
41	19.061024872169227	25.84309386477763	25.87741901089332	29.21846225215982
42	19.869209034528104	23.048914736149992	30.508227279231793	26.573648950090117
43	21.656361328747916	22.39271521258676	29.311991260649446	26.638932198015873
44	21.87409686051177	22.765428311417928	29.17833830751777	26.182136520552536
45	19.823099233342717	22.411795130318644	28.609868993917807	29.155236642420828
46	22.85783497180568	24.224387314847636	27.850881090024476	25.066896623322204
47	17.827564308208014	23.72260418430078	33.588043999786755	24.861787507704452
48	19.754823057145288	24.917717854781248	28.735852568353337	26.591606519720123
49	21.494369086043882	21.330412734161563	31.99636733331026	25.17885084648429
50	20.66804029603802	21.803108343068146	29.94490206109876	27.58394929979508
51	19.624724206336218	22.220995952999804	27.026516409196894	31.127763431467088
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	371.0
1	673.5
2	976.0
3	2930.5
4	4885.0
5	3283.5
6	1682.0
7	1755.5
8	1829.0
9	1828.5
10	1828.0
11	1876.0
12	1924.0
13	1978.5
14	2033.0
15	2119.5
16	2206.0
17	2115.0
18	2024.0
19	2206.0
20	2388.0
21	2716.5
22	3045.0
23	3629.5
24	4214.0
25	5027.0
26	7458.5
27	9077.0
28	10095.0
29	11113.0
30	13512.5
31	15912.0
32	18219.0
33	20526.0
34	23681.0
35	26836.0
36	28476.5
37	30117.0
38	33232.0
39	36347.0
40	40905.0
41	45463.0
42	50331.0
43	55199.0
44	63345.0
45	71491.0
46	106228.0
47	140965.0
48	131290.5
49	121616.0
50	118991.0
51	116366.0
52	97480.5
53	78595.0
54	66896.5
55	55198.0
56	50319.0
57	45440.0
58	43116.0
59	40792.0
60	36107.0
61	31422.0
62	28208.0
63	24994.0
64	21253.0
65	17512.0
66	15166.0
67	12820.0
68	10906.0
69	8992.0
70	7694.0
71	6396.0
72	5416.0
73	4436.0
74	3637.0
75	2210.5
76	1583.0
77	1146.0
78	709.0
79	716.5
80	724.0
81	495.0
82	266.0
83	180.0
84	94.0
85	73.5
86	53.0
87	37.0
88	21.0
89	22.5
90	24.0
91	13.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1069187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.843897108593627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03048455547837	22.21700854311317
2	11.577312096543883	7.141788461997517
3	4.316478158894357	3.994110246132876
4	2.223750132628081	2.743564811440078
5	1.321299550510182	2.037701369278353
6	0.8930414023274355	1.6526926276260945
7	0.6694085165675285	1.4453017186037598
8	0.5100789208081506	1.258625740053526
9	0.4436562519000997	1.2315679006671874
>10	5.717812459920532	41.666959065502645
>50	0.24008115583477843	4.513316617958046
>100	0.04807685041238695	2.7995411887586963
>500	0.003347122497018724	0.725859115414303
>1k	0.003955690223749401	2.5887473468415725
>5k	3.042838633653385E-4	0.5515740911934133
>10k+	9.128515900960156E-4	3.431641155418745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	12814	1.1984807147860943	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	11882	1.1113116788737611	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	11868	1.1100022727549064	No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	5877	0.5496699828935444	Illumina PCR Primer Index 3 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	4819	0.45071629191151785	RNA PCR Primer, Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	3006	0.28114819951982206	TruSeq Adapter, Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2763	0.2584206504568424	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT	2347	0.21951258292515716	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT	2211	0.20679263777056775	TruSeq Adapter, Index 7 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGTCACCT	2010	0.1879933070641525	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC	1857	0.1736833687652394	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	1737	0.162459887746484	TruSeq Adapter, Index 7 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTA	1679	0.1570352052540856	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1574	0.1472146593626746	No Hit
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1362	0.12738650956287345	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC	1200	0.11223481018755373	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.35290084896281E-5	0.0	0.0	0.2923716805385774	0.0
2	9.35290084896281E-5	0.0	0.0	1.568013827328615	0.0
3	9.35290084896281E-5	0.0	0.0	2.166131836619787	0.0
4	9.35290084896281E-5	0.0	0.0	2.9687977874777753	0.0
5	9.35290084896281E-5	0.0	0.0	5.645691539459421	0.0
6	9.35290084896281E-5	0.0	0.0	6.727728638675929	0.0
7	9.35290084896281E-5	0.0	0.0	7.986442034929343	0.0
8	9.35290084896281E-5	0.0	0.0	9.723462780598716	0.0
9	9.35290084896281E-5	0.0	0.0	10.43456383214536	0.0
10	1.870580169792562E-4	0.0	0.0	12.956199430034223	0.0
11	1.870580169792562E-4	0.0	0.0	14.779547450539521	0.0
12	1.870580169792562E-4	0.0	0.0	17.406777299013175	0.0
13	1.870580169792562E-4	0.0	0.0	18.091222583140272	0.0
14	1.870580169792562E-4	0.0	0.0	18.412214140276678	0.0
15	1.870580169792562E-4	0.0	0.0	19.01744035421306	0.0
16	1.870580169792562E-4	0.0	0.0	19.909613566195624	0.0
17	1.870580169792562E-4	0.0	0.0	20.92711564955429	0.0
18	1.870580169792562E-4	0.0	0.0	22.032067355850753	0.0
19	1.870580169792562E-4	0.0	0.0	22.89337599503174	0.0
20	1.870580169792562E-4	0.0	0.0	23.615513469580158	0.0
21	1.870580169792562E-4	0.0	0.0	24.385444267466777	0.0
22	1.870580169792562E-4	0.0	0.0	25.273502203075793	0.0
23	1.870580169792562E-4	0.0	0.0	26.047922393369916	0.0
24	2.8058702546888433E-4	0.0	0.0	26.694488429058715	0.0
25	2.8058702546888433E-4	0.0	0.0	27.252576022716326	0.0
26	4.6764504244814053E-4	0.0	0.0	27.803087766686275	0.0
27	4.6764504244814053E-4	0.0	0.0	28.485194825601134	0.0
28	4.6764504244814053E-4	0.0	0.0	29.045901231496455	0.0
29	4.6764504244814053E-4	0.0	0.0	29.66833678299493	0.0
30	4.6764504244814053E-4	0.0	0.0	30.431720550287274	0.0
31	4.6764504244814053E-4	0.0	0.0	31.024413877086047	0.0
32	4.6764504244814053E-4	0.0	0.0	31.554910413239217	0.0
33	4.6764504244814053E-4	9.35290084896281E-5	0.0	32.09195397998666	0.0
34	4.6764504244814053E-4	9.35290084896281E-5	0.0	32.65378273398386	0.0
35	4.6764504244814053E-4	9.35290084896281E-5	0.0	33.25339720741086	0.0
36	4.6764504244814053E-4	9.35290084896281E-5	0.0	33.79062783217529	0.0
37	5.611740509377687E-4	9.35290084896281E-5	0.0	34.348902483849876	0.0
38	5.611740509377687E-4	9.35290084896281E-5	0.0	34.96984157121252	0.0
39	5.611740509377687E-4	9.35290084896281E-5	0.0	35.89577875525984	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAT	20	7.0330664E-4	45.0	25
CGAATAT	85	0.0	45.0	14
GCGTACA	20	7.0330664E-4	45.0	2
GCTACGA	90	0.0	42.5	10
CGTTTTT	1615	0.0	41.23839	1
TACGGCT	1505	0.0	40.066444	7
GACGTAG	120	0.0	39.375004	1
GGTACGA	40	3.457917E-7	39.375	8
GCGTTAG	75	0.0	39.0	1
CGAGTAT	35	6.247881E-6	38.571426	27
TTGTACG	70	0.0	38.571426	1
CCGAGTA	35	6.247881E-6	38.571426	26
TACGGGA	310	0.0	38.46774	4
ACGGCTG	1570	0.0	38.407642	8
CTACGAA	100	0.0	38.250004	11
ACGTAGG	225	0.0	38.0	2
TTAGACG	30	1.13986855E-4	37.500004	1
CGTCTTA	30	1.13986855E-4	37.500004	17
CGCTCAT	30	1.13986855E-4	37.500004	21
CGCTATC	30	1.13986855E-4	37.500004	42
>>END_MODULE