FastQCFastQC Report
Sat 14 Jan 2017
SRR2936467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330449
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC94580.7108878280941245No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC75280.5658240188086878No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG68010.5111808118913239No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC59000.4434593133596252TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC37620.28276168421337455TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31500.23676217577674905No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT28360.21316112079455882TruSeq Adapter, Index 19 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT20630.15506043448489945No Hit
TCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC16210.1218385672806699TruSeq Adapter, Index 16 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT15610.11732881155158897No Hit
CGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG14710.11056417795796757No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG207.033687E-445.00000445
TAACGCG351.21212E-745.01
GCGTAAG1600.042.1875041
CGTTTTT17700.041.440681
ATAACGC501.0822987E-940.511
CACAACG3350.040.29850812
GCGTTAG950.040.2631571
CGTTAGG1800.040.02
GTACGTC451.9286745E-840.010
CGTAAGG2850.039.4736862
ATTGTCG403.458772E-739.3750041
TACGGCT10400.039.1586537
CGAATAT1500.039.014
GCTAACG659.094947E-1238.076921
CGTTGTA301.14001865E-437.49999611
CTATACG301.14001865E-437.4999961
ACGTAAG601.5643309E-1037.4999961
TAGTACG601.5643309E-1037.4999961
TGTAGCG850.037.0588261
ACGGGAT5250.036.857145
Produced by FastQC (version 0.11.5)