##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936467.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1330449
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.027957479016486	33.0	31.0	34.0	30.0	34.0
2	32.05160889293765	33.0	31.0	34.0	30.0	34.0
3	32.099099627268686	33.0	31.0	34.0	30.0	34.0
4	35.77656114589887	37.0	35.0	37.0	35.0	37.0
5	34.84245243523051	37.0	35.0	37.0	33.0	37.0
6	35.314916994187676	37.0	35.0	37.0	33.0	37.0
7	35.87581861461807	37.0	35.0	37.0	35.0	37.0
8	35.84233893971133	37.0	35.0	37.0	35.0	37.0
9	37.710572896819045	39.0	38.0	39.0	35.0	39.0
10	37.38040766688539	39.0	37.0	39.0	34.0	39.0
11	37.296732907462065	39.0	37.0	39.0	34.0	39.0
12	37.18918650771281	39.0	37.0	39.0	34.0	39.0
13	37.08574173079915	39.0	37.0	39.0	33.0	39.0
14	38.091200790109205	40.0	37.0	41.0	33.0	41.0
15	38.20824398379795	40.0	37.0	41.0	33.0	41.0
16	38.26049251042317	40.0	37.0	41.0	34.0	41.0
17	38.24478277634092	40.0	37.0	41.0	34.0	41.0
18	38.23089047381749	40.0	37.0	41.0	34.0	41.0
19	38.18504129057183	40.0	37.0	41.0	34.0	41.0
20	38.17704774854203	40.0	37.0	41.0	34.0	41.0
21	38.10067954502578	40.0	37.0	41.0	34.0	41.0
22	38.167979381396805	40.0	37.0	41.0	34.0	41.0
23	38.17716049243526	40.0	37.0	41.0	34.0	41.0
24	38.12653021649082	40.0	37.0	41.0	34.0	41.0
25	37.97438759396264	40.0	36.0	41.0	34.0	41.0
26	37.971072923501765	40.0	36.0	41.0	34.0	41.0
27	37.93993756994819	40.0	36.0	41.0	34.0	41.0
28	37.78891637334464	40.0	36.0	41.0	33.0	41.0
29	37.72052442446122	40.0	36.0	41.0	33.0	41.0
30	37.56068966191113	40.0	36.0	41.0	33.0	41.0
31	37.44379153203167	40.0	36.0	41.0	33.0	41.0
32	37.4042296998983	39.0	35.0	41.0	33.0	41.0
33	37.26386280120471	39.0	35.0	41.0	32.0	41.0
34	37.122072322952626	39.0	35.0	41.0	32.0	41.0
35	37.08182275307058	39.0	35.0	41.0	32.0	41.0
36	37.00225337461263	39.0	35.0	41.0	31.0	41.0
37	36.94644965722098	39.0	35.0	41.0	31.0	41.0
38	36.89163207308209	39.0	35.0	41.0	31.0	41.0
39	36.87685285193194	39.0	35.0	41.0	31.0	41.0
40	36.7819239970867	39.0	35.0	41.0	31.0	41.0
41	36.68144513619087	39.0	35.0	41.0	31.0	41.0
42	36.692839785666344	39.0	35.0	41.0	31.0	41.0
43	36.60354737385649	39.0	35.0	41.0	31.0	41.0
44	36.461348762710934	39.0	35.0	41.0	31.0	41.0
45	36.43381219422917	39.0	35.0	41.0	31.0	41.0
46	36.2930469337795	39.0	35.0	40.0	30.0	41.0
47	36.169384170306415	38.0	35.0	40.0	30.0	41.0
48	36.14565984866763	38.0	35.0	40.0	30.0	41.0
49	36.16263156272807	38.0	35.0	40.0	30.0	41.0
50	36.05815179687459	38.0	35.0	40.0	30.0	41.0
51	34.906632272262975	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	8.0
9	20.0
10	37.0
11	29.0
12	18.0
13	40.0
14	37.0
15	56.0
16	73.0
17	146.0
18	274.0
19	522.0
20	898.0
21	1601.0
22	2420.0
23	3714.0
24	5356.0
25	7396.0
26	9592.0
27	11231.0
28	13240.0
29	16303.0
30	19856.0
31	25812.0
32	35051.0
33	49105.0
34	86797.0
35	102551.0
36	102121.0
37	157005.0
38	273987.0
39	404882.0
40	268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.26362679065488	21.554753320119747	24.270678545363257	11.91094134386211
2	30.80997467772158	26.40875373652053	24.430474223363692	18.3507973623942
3	30.169739689383057	25.97446425981003	26.82921329566184	17.026582755145068
4	27.928466254625317	24.562459741034793	28.32231825496505	19.186755749374836
5	25.749502611524377	30.412514872798578	24.711732655667372	19.12624986000967
6	25.448626741799195	34.03580295073317	25.030873036095336	15.484697271372298
7	77.81831547094251	5.658540838468817	11.089714825596472	5.4334288649921945
8	77.63942849368898	6.607919582035839	9.714389653417756	6.038262270857432
9	71.93165615517769	6.641517262217492	11.71702184751163	9.70980473509319
10	40.26324947442555	24.928802231427134	19.08919470043572	15.718753593711595
11	29.693885297369533	23.665469326520597	25.919445239915245	20.72120013619462
12	26.758635618501724	21.032824257074115	30.514660840062263	21.6938792843619
13	24.623642093759322	22.69624765774562	32.64822627549046	20.031883973004604
14	20.21422842965044	26.218817857730738	30.878823615185553	22.688130097433273
15	18.50653426023846	24.63829879987884	35.60602473300367	21.249142206879032
16	21.88757329292592	25.614134777056467	29.729512367629273	22.768779562388335
17	22.27345805814428	24.70639611138796	29.100702093804422	23.919443736663336
18	22.80448179524356	24.67926241441799	30.48474612705936	22.03150966327909
19	22.890091991500615	26.825906141460514	27.265306674663968	23.018695192374906
20	23.6423944097068	27.71162216665201	28.788626997352022	19.85735642628917
21	22.73465574403829	27.42036710914887	29.974993404482248	19.869983742330596
22	21.698539365281945	23.69718794181513	29.06620246247695	25.53807023042597
23	21.10475486095296	26.452874180070037	29.12580640069631	23.3165645582807
24	22.56448762786097	24.688131600685182	28.6999351346801	24.04744563677375
25	21.8268419157743	27.55671205735808	26.34824784715536	24.268198179712265
26	21.03560527310705	26.710982532964433	27.112651443234576	25.140760750693943
27	21.8965176417886	27.066426447011498	27.551901651247064	23.485154259952843
28	18.689254529861724	27.545888643608286	28.627628717823832	25.13722810870616
29	21.262370823684336	27.280865331929295	27.60481611846828	23.851947725918095
30	21.49311999182231	28.507518890239307	27.224794035697723	22.774567082240658
31	21.4936461299907	28.901070240197107	24.83529996264419	24.769983667168002
32	22.818687525790164	27.88066284389706	25.520031207509646	23.78061842280313
33	21.70019294238261	26.256323992877594	24.077134861990203	27.966348202749597
34	19.532052713031465	26.77464525133996	27.17789257611528	26.51540945951329
35	18.90061174836465	27.642923554379013	26.178831356932886	27.277633340323455
36	20.831839476748076	29.27613159166567	23.709890420452044	26.182138511134212
37	19.384207887713096	28.175901518960895	27.415406377846878	25.024484215479138
38	19.77452724606505	26.829513946043782	25.558288968611347	27.837669839279823
39	20.776294318684897	23.3312964269957	26.98712990877516	28.905279345544248
40	22.07938823660283	22.620258273710604	27.824065409497095	27.47628808018947
41	19.486579342763232	24.97818405666057	25.143165953749445	30.392070646826745
42	20.311113015230198	24.28338102400017	28.319612401527607	27.08589355924203
43	21.183224610638966	24.025798809274164	27.834437847673982	26.956538732412895
44	20.9009139019985	24.07826230092247	27.682910055176862	27.33791374190217
45	20.72826542017018	23.082207585559463	27.406161378602263	28.783365615668092
46	22.10795002288701	24.593952868542875	26.87528796669395	26.422809141876165
47	18.462338654093468	24.569449862414867	30.698132735640378	26.27007874785129
48	19.612927665772983	24.48023186157455	28.16785912124403	27.73898135140843
49	21.065294498323496	22.220618753518547	30.153955544331275	26.560131203826675
50	20.036619216520137	22.632660101965577	29.162711235079286	28.168009446435
51	19.465684141218492	22.708423998214137	26.772240048284452	31.053651812282922
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	387.0
1	841.0
2	1295.0
3	3437.5
4	5580.0
5	3831.5
6	2083.0
7	2110.0
8	2137.0
9	2200.0
10	2263.0
11	2381.0
12	2499.0
13	2547.0
14	2595.0
15	2643.0
16	2691.0
17	2648.0
18	2605.0
19	2750.0
20	2895.0
21	3039.5
22	3184.0
23	3395.5
24	3607.0
25	5000.0
26	7854.5
27	9316.0
28	10304.5
29	11293.0
30	13331.5
31	15370.0
32	18689.0
33	22008.0
34	26655.5
35	31303.0
36	33952.5
37	36602.0
38	40668.5
39	44735.0
40	50264.5
41	55794.0
42	61378.5
43	66963.0
44	77893.5
45	88824.0
46	116413.5
47	144003.0
48	146969.5
49	149936.0
50	147794.5
51	145653.0
52	122156.0
53	98659.0
54	87528.5
55	76398.0
56	68081.5
57	59765.0
58	57257.0
59	54749.0
60	50947.5
61	47146.0
62	42307.5
63	37469.0
64	33387.0
65	29305.0
66	25129.5
67	20954.0
68	18479.5
69	16005.0
70	13396.0
71	10787.0
72	9127.0
73	7467.0
74	5991.5
75	3520.0
76	2524.0
77	1851.0
78	1178.0
79	930.0
80	682.0
81	613.0
82	544.0
83	361.5
84	179.0
85	109.0
86	39.0
87	37.0
88	35.0
89	27.0
90	19.0
91	13.5
92	8.0
93	6.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1330449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.08532026848577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.20559076033602	20.710374121711045
2	11.446668233877508	6.658600231788594
3	4.405026953711891	3.843648592200678
4	2.254207694740928	2.622574110128996
5	1.367238037962152	1.988327810869265
6	0.8818515162803267	1.5389360268157857
7	0.6991507352027537	1.4234516134523567
8	0.5637581932319611	1.311767008330758
9	0.43848062956644823	1.1478014588220677
>10	6.221258737159544	43.13500554676038
>50	0.4531620542500726	7.934004602990734
>100	0.05572135613704616	2.9609398820554
>500	0.004205385356434939	0.9359389371613699
>1k	0.002628365847771837	1.519152050908335
>5k	0.0010513463391087347	2.269478006004214
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	9458	0.7108878280941245	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC	7528	0.5658240188086878	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG	6801	0.5111808118913239	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	5900	0.4434593133596252	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	3762	0.28276168421337455	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3150	0.23676217577674905	No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT	2836	0.21316112079455882	TruSeq Adapter, Index 19 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	2063	0.15506043448489945	No Hit
TCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	1621	0.1218385672806699	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT	1561	0.11732881155158897	No Hit
CGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	1471	0.11056417795796757	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.261381683927756E-4	0.0	0.0	0.22954656661021955	0.0
2	5.261381683927756E-4	0.0	0.0	1.2423625407663128	0.0
3	5.261381683927756E-4	0.0	0.0	1.7732359526746233	0.0
4	5.261381683927756E-4	0.0	0.0	2.5390676380680506	0.0
5	5.261381683927756E-4	0.0	0.0	4.833330702642491	0.0
6	5.261381683927756E-4	0.0	0.0	6.026386580770852	0.0
7	5.261381683927756E-4	0.0	0.0	7.240037010062017	0.0
8	5.261381683927756E-4	0.0	0.0	9.030560359698116	0.0
9	5.261381683927756E-4	0.0	0.0	9.832094277946767	0.0
10	5.261381683927756E-4	0.0	0.0	11.825406310200542	0.0
11	5.261381683927756E-4	0.0	0.0	13.649827990400233	0.0
12	5.261381683927756E-4	0.0	0.0	15.631489820353881	0.0
13	5.261381683927756E-4	0.0	0.0	16.30712639116569	0.0
14	5.261381683927756E-4	0.0	0.0	16.62679290976204	0.0
15	5.261381683927756E-4	0.0	0.0	17.14699323311153	0.0
16	5.261381683927756E-4	0.0	0.0	17.93424625821809	0.0
17	6.01300763877458E-4	0.0	0.0	18.855965166646747	0.0
18	6.01300763877458E-4	0.0	0.0	19.866225612556363	0.0
19	6.01300763877458E-4	0.0	0.0	20.555692100937353	0.0
20	6.01300763877458E-4	0.0	0.0	21.20547273890243	0.0
21	6.01300763877458E-4	0.0	0.0	21.954543165502773	0.0
22	6.01300763877458E-4	0.0	0.0	22.714286680661942	0.0
23	6.01300763877458E-4	0.0	0.0	23.464634871385524	0.0
24	6.01300763877458E-4	0.0	0.0	24.070445390992063	0.0
25	6.01300763877458E-4	0.0	0.0	24.627325060938073	0.0
26	6.01300763877458E-4	0.0	0.0	25.15218546520761	0.0
27	6.01300763877458E-4	0.0	0.0	25.739280498538463	0.0
28	6.764633593621402E-4	0.0	0.0	26.263238951662185	0.0
29	6.764633593621402E-4	0.0	0.0	26.81365463839651	0.0
30	6.764633593621402E-4	0.0	0.0	27.477340356526256	0.0
31	6.764633593621402E-4	0.0	0.0	28.018135231038546	0.0
32	7.516259548468224E-4	0.0	0.0	28.545776651341015	0.0
33	8.267885503315046E-4	0.0	0.0	29.06289530827563	0.0
34	9.019511458161868E-4	0.0	0.0	29.590987704151004	0.0
35	9.019511458161868E-4	0.0	0.0	30.182517330615454	0.0
36	9.771137413008692E-4	0.0	0.0	30.68745964708155	0.0
37	0.0010522763367855513	0.0	0.0	31.22652578189769	0.0
38	0.0010522763367855513	0.0	0.0	31.786487118258574	0.0
39	0.0010522763367855513	0.0	0.0	32.52082567614392	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACG	20	7.033687E-4	45.000004	45
TAACGCG	35	1.21212E-7	45.0	1
GCGTAAG	160	0.0	42.187504	1
CGTTTTT	1770	0.0	41.44068	1
ATAACGC	50	1.0822987E-9	40.5	11
CACAACG	335	0.0	40.298508	12
GCGTTAG	95	0.0	40.263157	1
CGTTAGG	180	0.0	40.0	2
GTACGTC	45	1.9286745E-8	40.0	10
CGTAAGG	285	0.0	39.473686	2
ATTGTCG	40	3.458772E-7	39.375004	1
TACGGCT	1040	0.0	39.158653	7
CGAATAT	150	0.0	39.0	14
GCTAACG	65	9.094947E-12	38.07692	1
CGTTGTA	30	1.14001865E-4	37.499996	11
CTATACG	30	1.14001865E-4	37.499996	1
ACGTAAG	60	1.5643309E-10	37.499996	1
TAGTACG	60	1.5643309E-10	37.499996	1
TGTAGCG	85	0.0	37.058826	1
ACGGGAT	525	0.0	36.85714	5
>>END_MODULE