##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936466.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1014251
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.082988333262676	33.0	31.0	34.0	30.0	34.0
2	32.1006013304399	33.0	31.0	34.0	30.0	34.0
3	32.12937921678164	33.0	31.0	34.0	30.0	34.0
4	35.792683467898975	37.0	35.0	37.0	35.0	37.0
5	34.875496302197384	37.0	35.0	37.0	33.0	37.0
6	35.36566490937648	37.0	35.0	37.0	33.0	37.0
7	35.95403406060235	37.0	35.0	37.0	35.0	37.0
8	35.98058567356601	37.0	35.0	37.0	35.0	37.0
9	37.83568170009199	39.0	38.0	39.0	35.0	39.0
10	37.42490764120519	39.0	37.0	39.0	35.0	39.0
11	37.33643052853781	39.0	37.0	39.0	34.0	39.0
12	37.19544274543481	39.0	37.0	39.0	34.0	39.0
13	37.07740786057889	39.0	37.0	39.0	33.0	39.0
14	38.01038401736848	40.0	37.0	41.0	33.0	41.0
15	38.18617876640003	40.0	37.0	41.0	33.0	41.0
16	38.2501185603958	40.0	37.0	41.0	33.0	41.0
17	38.28660361192644	40.0	37.0	41.0	34.0	41.0
18	38.285316948171605	40.0	37.0	41.0	34.0	41.0
19	38.210699323934605	40.0	37.0	41.0	34.0	41.0
20	38.19623742052017	40.0	37.0	41.0	34.0	41.0
21	38.09526142937005	40.0	36.0	41.0	34.0	41.0
22	38.17794411836912	40.0	37.0	41.0	34.0	41.0
23	38.19251743404739	40.0	37.0	41.0	34.0	41.0
24	38.16256035241769	40.0	36.0	41.0	34.0	41.0
25	38.000295784771225	40.0	36.0	41.0	34.0	41.0
26	38.02545030766546	40.0	36.0	41.0	34.0	41.0
27	38.037829886290474	40.0	36.0	41.0	34.0	41.0
28	37.94848612424341	40.0	36.0	41.0	34.0	41.0
29	37.890603016413095	40.0	36.0	41.0	34.0	41.0
30	37.7483187100629	40.0	36.0	41.0	33.0	41.0
31	37.70597219031581	40.0	36.0	41.0	33.0	41.0
32	37.662439573636114	40.0	36.0	41.0	33.0	41.0
33	37.50876459574602	40.0	35.0	41.0	33.0	41.0
34	37.41904814488721	40.0	35.0	41.0	33.0	41.0
35	37.33513696313832	40.0	35.0	41.0	33.0	41.0
36	37.21524947966529	39.0	35.0	41.0	32.0	41.0
37	37.190553423166456	39.0	35.0	41.0	32.0	41.0
38	37.13590620073335	39.0	35.0	41.0	32.0	41.0
39	37.05556711307162	39.0	35.0	41.0	32.0	41.0
40	36.952564010289365	39.0	35.0	41.0	32.0	41.0
41	36.82886681896296	39.0	35.0	41.0	31.0	41.0
42	36.815429316806195	39.0	35.0	41.0	31.0	41.0
43	36.74014223303699	39.0	35.0	41.0	31.0	41.0
44	36.631720353245896	39.0	35.0	41.0	31.0	41.0
45	36.628799971604664	39.0	35.0	41.0	31.0	41.0
46	36.541102744784084	39.0	35.0	41.0	31.0	41.0
47	36.45548291300674	39.0	35.0	41.0	31.0	41.0
48	36.384230333517046	39.0	35.0	41.0	31.0	41.0
49	36.352246140255225	38.0	35.0	40.0	31.0	41.0
50	36.257362329443104	38.0	35.0	40.0	31.0	41.0
51	35.11031687422541	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	26.0
10	21.0
11	21.0
12	20.0
13	16.0
14	17.0
15	49.0
16	62.0
17	118.0
18	204.0
19	354.0
20	656.0
21	1004.0
22	1601.0
23	2333.0
24	3546.0
25	5028.0
26	6611.0
27	8036.0
28	9576.0
29	11400.0
30	14041.0
31	18253.0
32	24889.0
33	36755.0
34	69095.0
35	79964.0
36	73625.0
37	112285.0
38	200832.0
39	333594.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.07494298748534	22.758370462538366	26.26006777415058	11.906618775825708
2	31.12562866588251	24.123417181742983	27.225607862353602	17.525346290020913
3	31.69767641343218	24.099557210197474	27.850305299181365	16.35246107718898
4	28.613331414018816	25.559255056194175	27.531449315800526	18.29596421398648
5	24.35974921395197	31.027033742140752	26.06761048300667	18.545606560900605
6	26.02698937442507	32.67189285492447	25.847250828443848	15.453866942206613
7	81.40105358535511	5.3786488748840275	8.317664956702039	4.902632583058828
8	83.27485011106718	3.8980489050540745	7.943102841407107	4.883998142471636
9	77.07983526760141	6.573126376015405	10.342015930967777	6.005022425415405
10	41.85246058421436	24.709564003387722	18.26678011655892	15.171195295838999
11	30.398885482982024	25.34303638842851	25.262780120502715	18.995298008086756
12	28.26578430782913	21.899312891976443	29.67667766657366	20.158225133620768
13	25.28594992758203	23.298769239566933	31.52385356287546	19.89142726997558
14	20.959801863641246	25.749050284397057	31.61820890489632	21.672938947065372
15	19.240799368203728	25.311486012831143	34.83871349399705	20.609001124968078
16	23.723910550741383	25.095760319684178	29.93381322769216	21.246515901882276
17	24.260661315591506	24.25819644249796	28.35215346102691	23.128988780883628
18	24.89600700418338	24.283535337899593	30.561172727460956	20.25928493045607
19	24.629997899928124	26.386663656234994	27.73317452977616	21.25016391406072
20	23.845675281562453	28.359153700612573	27.72982230236894	20.065348715456036
21	23.33022102024055	26.817819257757698	30.45616913367598	19.39579058832577
22	22.769708878768668	23.864901291692096	29.73327115280143	23.63211867673781
23	21.267122240944303	24.7857778794401	30.271500841507677	23.675599038107926
24	22.074023096846837	24.773157729201152	30.690332077562655	22.462487096389356
25	22.24824032709852	26.329971575083484	28.302165834689834	23.11962226312816
26	20.31213180958165	27.546139959438047	27.900490115365923	24.24123811561438
27	20.971731849414002	27.19474765122243	28.820183564029023	23.013336935334546
28	18.9068583614904	27.328146583045026	29.610323282895457	24.154671772569117
29	22.15911051603597	26.480821808408372	28.613725793713783	22.74634188184187
30	24.009441449897512	26.602389349381955	27.855826614910907	21.532342585809626
31	23.87407061960008	27.520899658960158	27.342837226682548	21.262192494757215
32	26.64123574933621	26.732337458873594	25.557184562795598	21.069242228994597
33	25.846363474130168	25.726077667165224	26.714491777676336	21.713067081028267
34	23.093001633717886	25.07061861413003	30.035563188993653	21.800816563158428
35	24.129332877167485	27.03857329201549	27.252721466382585	21.579372364434445
36	25.36995280261001	27.389374030688657	27.30320206733836	19.937471099362977
37	24.120459334030727	26.859623505424203	29.052276014517115	19.967641146027955
38	25.28999231945544	25.59149559625773	27.529378822401952	21.58913326188488
39	25.41885588478592	23.601061275759154	28.23157186929074	22.74851097016419
40	27.24650998618685	23.247302689373736	27.898715406738567	21.607471917700845
41	23.039070210431145	24.32366347186249	27.973154574163594	24.664111743542772
42	22.558419957190086	24.26430932776995	30.046408630605242	23.13086208443472
43	22.600027015009104	24.204363614134962	30.105565584850297	23.090043786005634
44	23.151172638725523	23.720262538562938	28.402042492440234	24.7265223302713
45	23.2847687603956	23.869732442955442	27.46667245090219	25.378826345746763
46	24.25750627803177	24.864062248891052	27.829600365195596	23.04883110788158
47	21.64148716639175	24.681661639968805	30.916114452931275	22.760736740708168
48	22.41673905177318	24.53544536805978	29.902262852094797	23.145552728072243
49	24.253858265853324	22.9218408461022	30.47401481487324	22.350286073171237
50	23.176215749355926	22.968772029803276	29.489544501311805	24.365467719528993
51	22.25317007328561	23.568820735695603	27.67086253797137	26.50714665304742
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	330.0
1	584.0
2	838.0
3	2817.5
4	4797.0
5	3184.5
6	1572.0
7	1612.0
8	1652.0
9	1648.5
10	1645.0
11	1709.0
12	1773.0
13	1807.0
14	1841.0
15	1856.0
16	1871.0
17	1901.0
18	1931.0
19	1894.5
20	1858.0
21	2304.0
22	2750.0
23	3064.5
24	3379.0
25	4038.0
26	6156.0
27	7615.0
28	9405.5
29	11196.0
30	13473.0
31	15750.0
32	17859.5
33	19969.0
34	23270.5
35	26572.0
36	28621.5
37	30671.0
38	33337.5
39	36004.0
40	40921.0
41	45838.0
42	51255.0
43	56672.0
44	63510.0
45	70348.0
46	80250.5
47	90153.0
48	99246.0
49	108339.0
50	107116.5
51	105894.0
52	91277.0
53	76660.0
54	67589.0
55	58518.0
56	54866.0
57	51214.0
58	47702.5
59	44191.0
60	40298.0
61	36405.0
62	31948.5
63	27492.0
64	24539.0
65	21586.0
66	18196.0
67	14806.0
68	12582.5
69	10359.0
70	8750.5
71	7142.0
72	5802.5
73	4463.0
74	3558.0
75	2107.0
76	1561.0
77	1115.5
78	670.0
79	480.0
80	290.0
81	207.0
82	124.0
83	120.5
84	117.0
85	73.0
86	29.0
87	18.0
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1014251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.019927280039113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.61627843082346	22.52551676271216
2	11.143094295895812	6.913159494666134
3	4.132191251621823	3.8454081639756823
4	2.02578807443247	2.5135919501466266
5	1.1599173464871815	1.7990275869444148
6	0.8446024268053881	1.5719703516028618
7	0.6059460638626064	1.315748198565079
8	0.49850435143118116	1.2370854984142639
9	0.42860424144350334	1.1965745161344419
>10	6.125137126212887	46.048250457881075
>50	0.37423563919030595	6.89437401741682
>100	0.04090696664021687	2.2520553507705277
>500	0.002556685415005731	0.5068785054571022
>1k	0.0022370997381300148	1.3803591453128674
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	2784	0.27448826769704937	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2766	0.27271355906969774	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	2708	0.2669950534926759	No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT	2054	0.2025139733655673	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	1263	0.12452538868583812	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1262	0.12442679376209635	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	1087	0.10717268210728903	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9718984748351247E-4	0.0	0.0	0.21247206066348467	0.0
2	1.9718984748351247E-4	0.0	0.0	1.0082316901831991	0.0
3	1.9718984748351247E-4	0.0	0.0	1.4647261871075306	0.0
4	1.9718984748351247E-4	0.0	0.0	2.007885622000866	0.0
5	1.9718984748351247E-4	0.0	0.0	3.408327918828771	0.0
6	1.9718984748351247E-4	0.0	0.0	4.370712969472054	0.0
7	1.9718984748351247E-4	0.0	0.0	5.330337362250567	0.0
8	1.9718984748351247E-4	0.0	0.0	6.77278109659246	0.0
9	1.9718984748351247E-4	0.0	0.0	7.414239670456327	0.0
10	1.9718984748351247E-4	0.0	0.0	8.63770407916778	0.0
11	1.9718984748351247E-4	0.0	0.0	10.271619155416165	0.0
12	1.9718984748351247E-4	0.0	0.0	11.486702995609567	0.0
13	1.9718984748351247E-4	0.0	0.0	12.008664521898426	0.0
14	1.9718984748351247E-4	0.0	0.0	12.244503579488706	0.0
15	1.9718984748351247E-4	0.0	0.0	12.603191912061215	0.0
16	1.9718984748351247E-4	0.0	0.0	13.350147054328762	0.0
17	1.9718984748351247E-4	0.0	0.0	14.222120559900853	0.0
18	1.9718984748351247E-4	0.0	0.0	15.221281517099811	0.0
19	1.9718984748351247E-4	0.0	0.0	15.78938546769981	0.0
20	1.9718984748351247E-4	0.0	0.0	16.40570233600953	0.0
21	1.9718984748351247E-4	0.0	0.0	17.12544527932435	0.0
22	1.9718984748351247E-4	0.0	0.0	17.88896436878051	0.0
23	1.9718984748351247E-4	0.0	0.0	18.647849496820808	0.0
24	1.9718984748351247E-4	0.0	0.0	19.250165885959195	0.0
25	1.9718984748351247E-4	0.0	0.0	19.778930461986235	0.0
26	1.9718984748351247E-4	0.0	0.0	20.270228966991404	0.0
27	1.9718984748351247E-4	0.0	0.0	20.845628941948295	0.0
28	1.9718984748351247E-4	0.0	0.0	21.360294443880264	0.0
29	1.9718984748351247E-4	0.0	0.0	21.93953962086308	0.0
30	1.9718984748351247E-4	0.0	0.0	22.629901276902856	0.0
31	1.9718984748351247E-4	0.0	0.0	23.192286721925836	0.0
32	1.9718984748351247E-4	0.0	0.0	23.721938652266548	0.0
33	1.9718984748351247E-4	0.0	0.0	24.228815155222918	0.0
34	1.9718984748351247E-4	0.0	0.0	24.75994600941976	0.0
35	1.9718984748351247E-4	0.0	0.0	25.322331454442736	0.0
36	1.9718984748351247E-4	0.0	0.0	25.843602816265403	0.0
37	1.9718984748351247E-4	0.0	0.0	26.39879083185523	0.0
38	1.9718984748351247E-4	0.0	0.0	26.989965994610802	0.0
39	1.9718984748351247E-4	0.0	0.0	27.82467061900851	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGCA	25	3.8904793E-5	45.000004	14
CGATATA	25	3.8904793E-5	45.000004	33
TCGAAAG	25	3.8904793E-5	45.000004	1
TCGTTAG	20	7.032894E-4	45.0	1
TTTCGCG	20	7.032894E-4	45.0	1
TTATCCG	20	7.032894E-4	45.0	1
CTACGCG	20	7.032894E-4	45.0	1
ACTCGAT	20	7.032894E-4	45.0	19
TGTTACG	75	0.0	42.000004	1
ATTAACG	45	1.9279469E-8	40.0	1
CGGTATA	45	1.9279469E-8	40.0	7
CTTACGG	45	1.9279469E-8	40.0	2
CGTTTTT	1640	0.0	39.64939	1
GCGATAC	75	0.0	39.000004	9
CGACAGG	105	0.0	38.57143	2
TCGATAG	70	0.0	38.571426	1
ACGGGTC	100	0.0	38.250004	5
GCGATGT	160	0.0	37.96875	9
TAAACCG	30	1.1398273E-4	37.499996	1
CGAATCG	30	1.1398273E-4	37.499996	43
>>END_MODULE