FastQCFastQC Report
Sat 14 Jan 2017
SRR2936463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773298
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20610.26652079793300903No Hit
CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC19330.24996831751795556No Hit
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC17900.23147609330426305No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT13160.17018018926726824Illumina Single End Adapter 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC8440.10914291773675866No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG253.889617E-545.021
TCGACGC207.031854E-445.014
TAGTACG253.889617E-545.01
TACCGGT207.031854E-445.038
AATAGCG302.1645774E-644.9999961
TTGTGCG451.9268555E-840.01
GCAACCG403.4562618E-739.3751
CGTTTTT12500.039.061
ATTCGAT356.2456365E-638.5714342
TATACGG700.038.571432
ATTACGG601.546141E-1037.4999962
ACGGGTA1200.037.4999965
CCACGTA301.1395764E-437.49999618
ATACCGG301.1395764E-437.49999637
GTACGAG601.546141E-1037.4999961
ACCGAAC250.002106682936.017
AAGCGTG250.002106682936.01
CGTTAAT250.002106682936.031
TAACCCG250.002106682936.036
CGGATAG250.002106682936.024
Produced by FastQC (version 0.11.5)