##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936463.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 773298 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14992538452188 33.0 31.0 34.0 30.0 34.0 2 32.17151861248833 33.0 31.0 34.0 30.0 34.0 3 32.200215182245394 34.0 31.0 34.0 30.0 34.0 4 35.84041598452343 37.0 35.0 37.0 35.0 37.0 5 34.9997568854439 37.0 35.0 37.0 33.0 37.0 6 35.43769930867531 37.0 35.0 37.0 33.0 37.0 7 35.95850241433445 37.0 35.0 37.0 35.0 37.0 8 35.96591870145791 37.0 35.0 37.0 35.0 37.0 9 37.817003276873855 39.0 38.0 39.0 35.0 39.0 10 37.46181420357999 39.0 37.0 39.0 35.0 39.0 11 37.36709651389244 39.0 37.0 39.0 35.0 39.0 12 37.24013123013379 39.0 37.0 39.0 34.0 39.0 13 37.1461894379657 39.0 37.0 39.0 34.0 39.0 14 38.1687577104816 40.0 38.0 41.0 33.0 41.0 15 38.32664251039056 40.0 38.0 41.0 33.0 41.0 16 38.38046005550254 40.0 38.0 41.0 34.0 41.0 17 38.388129802482354 40.0 37.0 41.0 34.0 41.0 18 38.359580653253985 40.0 37.0 41.0 34.0 41.0 19 38.27008734019744 40.0 37.0 41.0 34.0 41.0 20 38.26313659158565 40.0 37.0 41.0 34.0 41.0 21 38.14433633605673 40.0 37.0 41.0 34.0 41.0 22 38.2111385261568 40.0 37.0 41.0 34.0 41.0 23 38.20521325543322 40.0 37.0 41.0 34.0 41.0 24 38.158466723048555 40.0 37.0 41.0 34.0 41.0 25 38.00405147821409 40.0 36.0 41.0 34.0 41.0 26 38.02649043447675 40.0 36.0 41.0 34.0 41.0 27 38.01971555596937 40.0 36.0 41.0 34.0 41.0 28 37.924365509803465 40.0 36.0 41.0 34.0 41.0 29 37.85248507043856 40.0 36.0 41.0 34.0 41.0 30 37.72317786933368 40.0 36.0 41.0 33.0 41.0 31 37.67860643632856 40.0 36.0 41.0 33.0 41.0 32 37.60542895494363 40.0 36.0 41.0 33.0 41.0 33 37.460942612033136 40.0 35.0 41.0 33.0 41.0 34 37.34355578315216 40.0 35.0 41.0 33.0 41.0 35 37.261509793119856 40.0 35.0 41.0 33.0 41.0 36 37.17644297541181 40.0 35.0 41.0 32.0 41.0 37 37.12743082227033 40.0 35.0 41.0 32.0 41.0 38 37.01799564980124 39.0 35.0 41.0 32.0 41.0 39 36.933889651854784 39.0 35.0 41.0 32.0 41.0 40 36.79752695597299 39.0 35.0 41.0 31.0 41.0 41 36.70232820982338 39.0 35.0 41.0 31.0 41.0 42 36.70993588500164 39.0 35.0 41.0 31.0 41.0 43 36.59679062922702 39.0 35.0 41.0 31.0 41.0 44 36.44871705345158 39.0 35.0 41.0 31.0 41.0 45 36.39728927269953 39.0 35.0 41.0 31.0 41.0 46 36.31049478984816 39.0 35.0 41.0 31.0 41.0 47 36.21629436517358 38.0 35.0 41.0 30.0 41.0 48 36.179117235528864 38.0 35.0 41.0 30.0 41.0 49 36.12914426262579 38.0 35.0 40.0 30.0 41.0 50 36.012018652576366 38.0 35.0 40.0 30.0 41.0 51 34.88342527718939 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 10.0 9 18.0 10 26.0 11 16.0 12 20.0 13 26.0 14 25.0 15 39.0 16 65.0 17 114.0 18 189.0 19 368.0 20 589.0 21 1004.0 22 1452.0 23 2179.0 24 3175.0 25 4296.0 26 5714.0 27 6746.0 28 7656.0 29 8879.0 30 10819.0 31 13832.0 32 18334.0 33 26863.0 34 49368.0 35 60715.0 36 56428.0 37 86248.0 38 149640.0 39 258265.0 40 179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.66556230586398 22.620128333449717 25.17683997630926 12.537469384377046 2 31.66786930782182 24.908638067084098 26.24059547548293 17.182897149611147 3 32.067456530341474 24.29412723167524 27.13947275177228 16.498943486211008 4 28.210340644874293 26.084898706578834 27.151111214564118 18.55364943398276 5 25.051403210663935 31.31186166264493 24.969546022361367 18.66718910432977 6 26.440389086742755 33.33863529971628 24.958166192076018 15.262809421464945 7 79.92572074413745 6.3736101735682755 9.06584524982607 4.634823832468207 8 81.13275865190393 5.370374680912145 8.601599900685116 4.895266766498814 9 74.97678773254295 7.347749509244819 10.95321596590189 6.722246792310338 10 41.69660337929233 24.58444222020489 18.39678881879948 15.3221655817033 11 30.996330004732975 25.91898595366857 24.38089843760103 18.703785603997424 12 27.476729540228995 23.101960693031664 29.420094193958864 20.00121557278048 13 25.56685779608896 24.09148866284408 30.31910078650145 20.02255275456551 14 20.67288419212257 26.418922588704486 31.79227154344121 21.115921675731737 15 19.73663451864611 24.527025803765174 34.84077289738238 20.895566780206337 16 24.393442114165563 24.51564597347982 29.962575876311593 21.128336036043027 17 24.39732160176284 24.012347115859605 28.508931873611466 23.08139940876609 18 25.3702970911602 24.268005348520234 29.449061034685204 20.912636525634362 19 25.44530051804091 26.42176754627582 26.943817260616214 21.18911467506705 20 25.66707789235198 26.87088289378739 27.68635118673526 19.775688027125376 21 24.115541485947205 26.38853326919247 29.770541240246324 19.725384004614003 22 23.824838548657826 24.020881988573617 28.825756694055848 23.32852276871271 23 21.967727835840776 25.821740131230136 29.375997351603132 22.83453468132596 24 22.224291282274102 25.405600428295433 29.885632705632243 22.484475583798226 25 22.548875077913042 27.031235047808217 27.056322400937283 23.363567473341455 26 20.96462165943789 27.679756057819883 27.378061239004886 23.977561043737346 27 21.95453757801003 27.12279095510398 28.70264762096889 22.220023845917098 28 20.463521178122793 26.992181539328953 28.66087847117153 23.88341881137673 29 21.685301138758923 27.2964626832088 28.126285080266598 22.891951097765673 30 23.04506154160492 26.655054067125484 28.43845451559425 21.861429875675352 31 23.81410529963869 26.942653414337038 26.7561793771612 22.487061908863076 32 24.87488652498778 27.28624669920264 25.847603381878653 21.99126339393093 33 24.688800436571672 26.02153374248996 26.609017480971115 22.680648339967256 34 21.99617741155415 25.929072621421493 29.359315554934838 22.715434412089518 35 22.983894953821167 27.319868925045714 27.473496633897927 22.222739487235195 36 24.070539429818776 28.195986540764363 26.63177714154181 21.10169688787505 37 23.156014886887075 27.102617619598135 27.839202998068014 21.902164495446776 38 24.372880829899987 26.576171152647493 26.5953099581274 22.455638059325125 39 24.16300055088724 24.569053586069018 28.40935835861466 22.85858750442908 40 25.268783832364754 24.0071744657299 29.362548461265902 21.361493240639444 41 22.89531332034998 25.24266194920975 27.720490677591304 24.141534052848968 42 24.05528011193615 25.336933497823605 27.940069675597247 22.667716714642996 43 23.554308946874297 24.855747719507875 28.445825542029073 23.14411779158875 44 22.4901654989409 24.766907453530205 28.53893324436375 24.203993803165144 45 22.437275151364673 24.85109233439114 28.08580909300166 24.625823421242522 46 23.17515369236698 25.168175787342008 28.392159296933393 23.26451122335762 47 21.18394202493735 25.637076521599695 29.976671347915033 23.202310105547927 48 22.292828896492683 25.456938980832746 28.697604287092425 23.552627835582143 49 23.735481017667187 24.14179268535545 29.67135567400924 22.451370622968117 50 22.18252213247674 24.220546283580198 29.578118655421328 24.018812928521736 51 22.0713101546881 24.070927378578507 27.999296519582362 25.858465947151032 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 315.0 1 638.5 2 962.0 3 2395.0 4 3828.0 5 2637.5 6 1447.0 7 1479.0 8 1511.0 9 1572.5 10 1634.0 11 1648.0 12 1662.0 13 1616.0 14 1570.0 15 1724.5 16 1879.0 17 1822.0 18 1765.0 19 1769.5 20 1774.0 21 2193.5 22 2613.0 23 3080.0 24 3547.0 25 3678.5 26 5510.0 27 7210.0 28 8306.5 29 9403.0 30 11014.5 31 12626.0 32 14301.5 33 15977.0 34 19190.0 35 22403.0 36 23787.5 37 25172.0 38 27754.5 39 30337.0 40 32739.0 41 35141.0 42 39325.0 43 43509.0 44 47044.0 45 50579.0 46 58205.0 47 65831.0 48 70785.0 49 75739.0 50 73085.5 51 70432.0 52 62335.0 53 54238.0 54 48441.5 55 42645.0 56 40094.5 57 37544.0 58 36224.0 59 34904.0 60 31041.0 61 27178.0 62 25192.0 63 23206.0 64 20236.5 65 17267.0 66 15415.0 67 13563.0 68 11950.0 69 10337.0 70 8568.0 71 6799.0 72 6125.0 73 5451.0 74 4515.5 75 2766.0 76 1952.0 77 1463.5 78 975.0 79 691.5 80 408.0 81 380.5 82 353.0 83 239.0 84 125.0 85 81.5 86 38.0 87 35.0 88 32.0 89 20.0 90 8.0 91 9.5 92 11.0 93 8.5 94 6.0 95 3.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 773298.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.21955774202038 #Duplication Level Percentage of deduplicated Percentage of total 1 73.79968119167073 23.039934083060597 2 9.855067344506567 6.153416880266445 3 3.468862030721099 3.248890154015982 4 1.7591540563091146 2.196800465522071 5 1.1120138480954844 1.735829027027163 6 0.8073518645084904 1.5123100891290377 7 0.6556390240900412 1.432813225935065 8 0.5424229815090449 1.3547364473456385 9 0.4850965400395594 1.3630049497997394 >10 7.288356957339401 51.28002550916343 >50 0.19175490645688872 3.6252182158992436 >100 0.030013811352515046 1.5555798828966998 >500 0.0029180094370500737 0.5774372910911618 >1k 0.0016674339640286137 0.9240037788477009 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2061 0.26652079793300903 No Hit CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC 1933 0.24996831751795556 No Hit GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC 1790 0.23147609330426305 No Hit CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT 1316 0.17018018926726824 Illumina Single End Adapter 2 (95% over 21bp) GAATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC 844 0.10914291773675866 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.586325064852101E-4 0.0 0.0 0.17780984820858195 0.0 2 2.586325064852101E-4 0.0 0.0 0.8402970135704476 0.0 3 2.586325064852101E-4 0.0 0.0 1.1838902984360493 0.0 4 2.586325064852101E-4 0.0 0.0 1.6094700878574624 0.0 5 2.586325064852101E-4 0.0 0.0 2.692364392511037 0.0 6 2.586325064852101E-4 0.0 0.0 3.4434331913440874 0.0 7 2.586325064852101E-4 0.0 0.0 4.1506637803278945 0.0 8 2.586325064852101E-4 0.0 0.0 5.287482962583636 0.0 9 2.586325064852101E-4 0.0 0.0 5.799704641677594 0.0 10 2.586325064852101E-4 0.0 0.0 6.802422869320753 0.0 11 2.586325064852101E-4 0.0 0.0 8.027694368794437 0.0 12 2.586325064852101E-4 0.0 0.0 9.06235371098852 0.0 13 2.586325064852101E-4 0.0 0.0 9.527116325142442 0.0 14 2.586325064852101E-4 0.0 0.0 9.753678400823485 0.0 15 2.586325064852101E-4 0.0 0.0 10.080589889020791 0.0 16 2.586325064852101E-4 0.0 0.0 10.656305848456869 0.0 17 2.586325064852101E-4 0.0 0.0 11.329526262837872 0.0 18 2.586325064852101E-4 0.0 0.0 12.083827968001986 0.0 19 2.586325064852101E-4 0.0 0.0 12.548590582155908 0.0 20 2.586325064852101E-4 0.0 0.0 13.013999777576045 0.0 21 2.586325064852101E-4 0.0 0.0 13.574456419129495 0.0 22 2.586325064852101E-4 0.0 0.0 14.157672721253643 0.0 23 2.586325064852101E-4 0.0 0.0 14.729250560585958 0.0 24 2.586325064852101E-4 0.0 0.0 15.213151980219786 0.0 25 3.8794875972781516E-4 0.0 0.0 15.632136640725827 0.0 26 3.8794875972781516E-4 0.0 0.0 16.04038805221273 0.0 27 5.172650129704202E-4 0.0 0.0 16.511616479028785 0.0 28 5.172650129704202E-4 0.0 0.0 16.930601139534822 0.0 29 5.172650129704202E-4 0.0 0.0 17.392518796117407 0.0 30 5.172650129704202E-4 0.0 0.0 17.93461252971041 0.0 31 5.172650129704202E-4 0.0 0.0 18.383986509728462 0.0 32 5.172650129704202E-4 0.0 0.0 18.833489805999758 0.0 33 5.172650129704202E-4 0.0 0.0 19.252215833999312 0.0 34 6.465812662130253E-4 0.0 0.0 19.689822034972288 0.0 35 6.465812662130253E-4 0.0 0.0 20.162214308067522 0.0 36 6.465812662130253E-4 0.0 0.0 20.591673585086216 0.0 37 6.465812662130253E-4 0.0 0.0 21.034840384948623 0.0 38 7.758975194556303E-4 0.0 0.0 21.500637529128486 0.0 39 9.052137726982353E-4 0.0 0.0 22.161702215704683 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATCG 25 3.889617E-5 45.0 21 TCGACGC 20 7.031854E-4 45.0 14 TAGTACG 25 3.889617E-5 45.0 1 TACCGGT 20 7.031854E-4 45.0 38 AATAGCG 30 2.1645774E-6 44.999996 1 TTGTGCG 45 1.9268555E-8 40.0 1 GCAACCG 40 3.4562618E-7 39.375 1 CGTTTTT 1250 0.0 39.06 1 ATTCGAT 35 6.2456365E-6 38.57143 42 TATACGG 70 0.0 38.57143 2 ATTACGG 60 1.546141E-10 37.499996 2 ACGGGTA 120 0.0 37.499996 5 CCACGTA 30 1.1395764E-4 37.499996 18 ATACCGG 30 1.1395764E-4 37.499996 37 GTACGAG 60 1.546141E-10 37.499996 1 ACCGAAC 25 0.0021066829 36.0 17 AAGCGTG 25 0.0021066829 36.0 1 CGTTAAT 25 0.0021066829 36.0 31 TAACCCG 25 0.0021066829 36.0 36 CGGATAG 25 0.0021066829 36.0 24 >>END_MODULE