##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936461.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1105876
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01789260278729	33.0	31.0	34.0	30.0	34.0
2	32.07171057152882	33.0	31.0	34.0	30.0	34.0
3	32.10034488495998	33.0	31.0	34.0	30.0	34.0
4	35.759543565463034	37.0	35.0	37.0	35.0	37.0
5	34.8375116197476	37.0	35.0	37.0	33.0	37.0
6	35.298807461234354	37.0	35.0	37.0	33.0	37.0
7	35.850768983140966	37.0	35.0	37.0	35.0	37.0
8	35.81735926993623	37.0	35.0	37.0	35.0	37.0
9	37.672023807370806	39.0	37.0	39.0	35.0	39.0
10	37.36744716405818	39.0	37.0	39.0	34.0	39.0
11	37.25235198159649	39.0	37.0	39.0	34.0	39.0
12	37.08727289497195	39.0	37.0	39.0	34.0	39.0
13	36.96551602530483	39.0	37.0	39.0	33.0	39.0
14	37.90636111101064	40.0	37.0	41.0	33.0	41.0
15	38.032614868213074	40.0	37.0	41.0	33.0	41.0
16	38.13857068966141	40.0	37.0	41.0	33.0	41.0
17	38.10882684857977	40.0	37.0	41.0	33.0	41.0
18	38.095412143857	40.0	37.0	41.0	34.0	41.0
19	38.056225110229356	40.0	37.0	41.0	34.0	41.0
20	38.004867634345985	40.0	36.0	41.0	33.0	41.0
21	37.93721176696121	40.0	36.0	41.0	33.0	41.0
22	38.01109256372324	40.0	36.0	41.0	34.0	41.0
23	38.000524471098025	40.0	36.0	41.0	34.0	41.0
24	37.95918258466591	40.0	36.0	41.0	34.0	41.0
25	37.8239902122842	40.0	36.0	41.0	33.0	41.0
26	37.8406873826722	40.0	36.0	41.0	34.0	41.0
27	37.81539973740275	40.0	36.0	41.0	34.0	41.0
28	37.72198148797876	40.0	36.0	41.0	33.0	41.0
29	37.66391620760375	40.0	36.0	41.0	33.0	41.0
30	37.506727698222946	40.0	35.0	41.0	33.0	41.0
31	37.48874105234221	40.0	35.0	41.0	33.0	41.0
32	37.43680032842742	40.0	35.0	41.0	33.0	41.0
33	37.3207683320734	40.0	35.0	41.0	33.0	41.0
34	37.230847762316934	40.0	35.0	41.0	32.0	41.0
35	37.12564971117919	40.0	35.0	41.0	32.0	41.0
36	37.0466734064217	39.0	35.0	41.0	32.0	41.0
37	36.9952164618818	39.0	35.0	41.0	31.0	41.0
38	36.941908496070084	39.0	35.0	41.0	31.0	41.0
39	36.89689802473333	39.0	35.0	41.0	31.0	41.0
40	36.748712333028294	39.0	35.0	41.0	31.0	41.0
41	36.57916529520489	39.0	35.0	41.0	31.0	41.0
42	36.66536121590486	39.0	35.0	41.0	31.0	41.0
43	36.56974470917174	39.0	35.0	41.0	31.0	41.0
44	36.47836556720645	39.0	35.0	41.0	31.0	41.0
45	36.43979614350976	39.0	35.0	41.0	31.0	41.0
46	36.313856164705626	39.0	35.0	41.0	31.0	41.0
47	36.17076055543298	38.0	35.0	40.0	30.0	41.0
48	36.11691184183398	38.0	35.0	40.0	30.0	41.0
49	36.106664761691185	38.0	35.0	40.0	30.0	41.0
50	35.98901052197534	38.0	35.0	40.0	30.0	41.0
51	34.76162969446846	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	26.0
10	29.0
11	18.0
12	22.0
13	26.0
14	28.0
15	36.0
16	82.0
17	139.0
18	284.0
19	585.0
20	970.0
21	1677.0
22	2544.0
23	3661.0
24	4702.0
25	6283.0
26	7875.0
27	9518.0
28	11432.0
29	13391.0
30	17012.0
31	21820.0
32	29273.0
33	42598.0
34	76823.0
35	89626.0
36	81276.0
37	122333.0
38	209117.0
39	352437.0
40	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6004579175242	20.93950858866636	24.089047958360613	11.37098553544882
2	31.217695293143173	27.054299035334882	24.012909223095537	17.715096448426408
3	31.299982999902337	26.425385847961252	25.74963196597087	16.524999186165537
4	28.041841942496266	23.689183959141893	28.87159138999309	19.39738270836875
5	27.020208413963232	29.08644368808076	24.650051181145084	19.243296716810924
6	25.428890761712886	33.96845577623531	25.61733865279652	14.985314809255287
7	76.63978601579201	6.063247597379815	12.184187015542431	5.11277937128575
8	76.62848275936905	7.591357439712952	9.991355269487718	5.788804531430287
9	70.1931319605453	7.365021033099552	11.96372830226897	10.478118704086173
10	42.38205730118024	22.737901898585374	19.320249286538456	15.55979151369593
11	32.04066278678622	23.9298981079253	24.635673438975076	19.393765666313403
12	28.499849892754703	20.878290151879597	29.43883401032304	21.18302594504266
13	26.341199194123032	22.672614289486344	32.24240330742326	18.743783208967372
14	20.996838705243626	26.917122715385812	30.94189583642289	21.144142742947672
15	18.611218617638865	24.555103827192198	35.81983875226517	21.013838802903763
16	23.182345941136255	24.582593346812843	29.074778727452266	23.160281984598637
17	23.716583052711155	24.621295696805067	27.82192578553111	23.84019546495267
18	24.356166514148057	23.944908832454995	29.872336500656495	21.826588152740452
19	24.239245629708936	25.29949108218281	26.766020783523647	23.695242504584602
20	25.111676173458868	27.177007187062564	28.245752688366508	19.46556395111206
21	23.768668458308166	26.934574943302863	30.081039827250073	19.2157167711389
22	22.503065443141907	22.520155966853427	30.333780640867513	24.642997949137154
23	21.701257645522645	25.406193822815577	30.10961445948732	22.782934072174456
24	24.17450057691821	24.077835127989033	28.373886403177213	23.373777891915548
25	22.269766230571964	27.384806253142308	26.726052468812057	23.619375047473675
26	21.998216798266714	25.232214099953342	27.518546383138798	25.251022718641146
27	22.124541992049743	25.185915961644884	29.52329194231541	23.16625010398996
28	18.90293305940268	25.022335234691774	31.11054042225349	24.964191283652053
29	23.415283449500667	23.710524507268445	28.78206959912323	24.09212244410766
30	23.373777891915548	23.91814272124542	31.743341929836618	20.964737457002414
31	24.911382469643975	23.540885234872626	28.884251037186807	22.66348125829659
32	25.167469047162612	24.025568870289256	26.734281239487974	24.072680843060162
33	23.662869978189235	22.29481424680525	29.505749288346976	24.53656648665854
34	24.131367350408183	22.255388488401955	30.317503951618445	23.295740209571417
35	22.521150653418648	23.105755075614265	29.270098998441057	25.102995272526034
36	24.659455490488988	23.018765214183144	31.151051293273387	21.170728002054478
37	21.247318867576475	24.310049227942372	31.006007906853934	23.43662399762722
38	21.067913581631213	24.263389385428386	29.667250216118262	25.00144681682214
39	23.822019828624548	21.865109650629908	29.057688203740746	25.2551823170048
40	24.90152603004315	20.99276953293136	30.124173053760096	23.98153138326539
41	22.343373036398294	23.11597321942062	28.04690580137375	26.49374794280733
42	21.32906401802734	21.49146920631246	31.271227515562323	25.908239260097876
43	22.543757166264573	21.79991246758226	31.136130994795074	24.52019937135809
44	22.57594884055717	21.29415956219323	29.830288386763073	26.299603210486527
45	21.711385363277618	21.558565336439166	28.23444943194355	28.49559986833967
46	24.557454904528175	22.052291576994165	28.835149691285462	24.555103827192198
47	20.06599293229982	22.923456156024727	33.34578198640716	23.664768925268294
48	20.9947589060618	22.85165787122607	30.449616412690027	25.7039668100221
49	22.282154599611527	20.302547482719582	33.236276038181494	24.17902187948739
50	21.715725813744037	21.010583465053948	31.22709960248708	26.046591118714936
51	20.955513999761273	21.71238004984284	28.577345018790535	28.75476093160535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	398.0
1	817.5
2	1237.0
3	2710.5
4	4184.0
5	2895.0
6	1606.0
7	1596.0
8	1586.0
9	1634.0
10	1682.0
11	1701.5
12	1721.0
13	1755.5
14	1790.0
15	1852.5
16	1915.0
17	1888.5
18	1862.0
19	2065.5
20	2269.0
21	2451.0
22	2633.0
23	3158.5
24	3684.0
25	4697.5
26	6404.5
27	7098.0
28	8275.5
29	9453.0
30	11973.5
31	14494.0
32	17365.5
33	20237.0
34	23208.5
35	26180.0
36	27655.0
37	29130.0
38	32370.5
39	35611.0
40	39878.5
41	44146.0
42	50132.5
43	56119.0
44	63780.0
45	71441.0
46	95989.0
47	120537.0
48	118553.0
49	116569.0
50	114661.5
51	112754.0
52	96331.0
53	79908.0
54	73705.0
55	67502.0
56	62506.0
57	57510.0
58	54329.5
59	51149.0
60	45722.0
61	40295.0
62	36141.0
63	31987.0
64	28771.5
65	25556.0
66	22031.0
67	18506.0
68	16081.0
69	13656.0
70	11670.0
71	9684.0
72	7911.5
73	6139.0
74	4788.5
75	2814.5
76	2191.0
77	1716.5
78	1242.0
79	908.5
80	575.0
81	387.0
82	199.0
83	207.0
84	215.0
85	125.5
86	36.0
87	26.5
88	17.0
89	12.5
90	8.0
91	7.0
92	6.0
93	5.0
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1105876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.291362818370544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.24360738295006	19.443351375776327
2	12.11369741174193	6.611986222716104
3	4.432035422732692	3.6286886023700458
4	2.188270236289799	2.388835078529054
5	1.3342288597434777	1.820646194700004
6	0.83806850314509	1.3723218951588823
7	0.6339891383653754	1.2111699318624911
8	0.47954127893246534	1.0469868023785074
9	0.39482163024706146	0.9697698323651789
>10	5.359683935807937	37.379853517201454
>50	0.8997461566683471	15.15052679245751
>100	0.07397906732569993	3.717578222727913
>500	0.0026659123360597407	0.5217541958505539
>1k	0.004332107546097079	1.9863401414627762
>5k	0.0013329561680298703	2.7501911944432194
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC	8700	0.786706647038185	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC	8392	0.7588554232120057	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG	7852	0.710025355464808	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	5296	0.47889636812807224	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	3582	0.3239061160564114	No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT	2296	0.2076182139769739	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2265	0.20481500638407923	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1758	0.1589689983325436	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT	1727	0.15616579073964892	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT	1469	0.13283586948265447	No Hit
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	1462	0.1322028871229686	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT	1392	0.1258730635261096	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTC	1300	0.11755386679880926	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTA	1226	0.11086233899641551	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTCTGCTT	1180	0.10670274063276533	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG	1107	0.10010163888175527	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8085210276739888E-4	0.0	0.0	0.22091084353037774	0.0
2	1.8085210276739888E-4	0.0	0.0	1.3256459132850338	0.0
3	1.8085210276739888E-4	0.0	0.0	1.8517446802353972	0.0
4	1.8085210276739888E-4	0.0	0.0	2.5714456231982608	0.0
5	1.8085210276739888E-4	0.0	0.0	4.877581211636747	0.0
6	1.8085210276739888E-4	0.0	0.0	5.923901052197534	0.0
7	1.8085210276739888E-4	0.0	0.0	6.9371249579518866	0.0
8	2.712781541510983E-4	0.0	0.0	8.20706842358456	0.0
9	2.712781541510983E-4	0.0	0.0	8.635235776886377	0.0
10	3.6170420553479775E-4	0.0	0.0	10.331538074793196	0.0
11	3.6170420553479775E-4	0.0	0.0	11.902419439430822	0.0
12	3.6170420553479775E-4	0.0	0.0	13.90526605152838	0.0
13	3.6170420553479775E-4	0.0	0.0	14.434168026071639	0.0
14	3.6170420553479775E-4	0.0	0.0	14.68962162123059	0.0
15	4.521302569184972E-4	0.0	0.0	15.205411818323212	0.0
16	5.425563083021966E-4	0.0	0.0	16.027746329606575	0.0
17	5.425563083021966E-4	0.0	0.0	16.90442689777154	0.0
18	5.425563083021966E-4	0.0	0.0	17.810586358687594	0.0
19	6.329823596858961E-4	0.0	0.0	18.528207502468632	0.0
20	6.329823596858961E-4	0.0	0.0	19.143285503980554	0.0
21	7.234084110695955E-4	0.0	0.0	19.86298644694342	0.0
22	7.234084110695955E-4	0.0	0.0	20.62735785928983	0.0
23	7.234084110695955E-4	0.0	0.0	21.324633141509537	0.0
24	7.234084110695955E-4	0.0	0.0	21.883104434855262	0.0
25	7.234084110695955E-4	0.0	0.0	22.397719093279896	0.0
26	7.234084110695955E-4	0.0	0.0	22.927254050182842	0.0
27	7.234084110695955E-4	0.0	0.0	23.501097772263797	0.0
28	8.13834462453295E-4	0.0	0.0	24.007574086063897	0.0
29	8.13834462453295E-4	0.0	0.0	24.53756117322376	0.0
30	8.13834462453295E-4	0.0	0.0	25.162405188285124	0.0
31	9.042605138369944E-4	0.0	0.0	25.681541149278942	0.0
32	9.042605138369944E-4	0.0	0.0	26.161884334229153	0.0
33	9.042605138369944E-4	0.0	0.0	26.68590330199769	0.0
34	9.042605138369944E-4	0.0	0.0	27.23759264148964	0.0
35	9.042605138369944E-4	0.0	0.0	27.798143734017195	0.0
36	9.946865652206939E-4	0.0	0.0	28.28834335856823	0.0
37	9.946865652206939E-4	0.0	0.0	28.787495162206252	0.0
38	9.946865652206939E-4	0.0	0.0	29.32616315029895	0.0
39	9.946865652206939E-4	0.0	0.0	30.080135566736235	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	25	3.89071E-5	45.0	1
ATTACCG	20	7.033172E-4	45.0	1
ACCCGTT	35	1.2118653E-7	45.0	39
TATACGA	20	7.033172E-4	45.0	39
TCACGAC	105	0.0	38.57143	25
CCCGTTC	35	6.248074E-6	38.571426	40
TACGGCT	1125	0.0	38.4	7
CGTTTTT	1505	0.0	38.272423	1
GCAACGA	95	0.0	37.894737	11
GTACGAG	95	0.0	37.894737	1
TAGACGG	150	0.0	37.5	2
CGCATCG	60	1.5643309E-10	37.499996	21
TACGGAT	30	1.13989394E-4	37.499996	13
CGTAGTC	30	1.13989394E-4	37.499996	38
CGTAAGG	110	0.0	36.818184	2
TACGGGA	295	0.0	36.61017	4
AACGGGA	240	0.0	36.5625	4
TCGACGT	105	0.0	36.42857	26
GGTACGA	25	0.0021070743	36.0	8
ACACGCG	450	0.0	36.0	36
>>END_MODULE