FastQCFastQC Report
Sat 14 Jan 2017
SRR2936460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463050
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282681.932128088582072No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT99760.6818632309217046No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTC74960.5123543282867982No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT57020.38973377533235365No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG47410.3240490755613274No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCT39380.2691637332968798No Hit
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC26710.18256382215235295TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTC25440.17388332592871056No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCC21150.14456101978743036No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGCT20280.1386145381224155TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGT18100.12371415877789549No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCG14670.10026998393766447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT178300.044.07881
CGTTATT4900.041.7857131
CGTTTCT11150.041.3677141
CCATACG1700.041.029412
TCACGAC556.184564E-1140.90909225
GAACCCG1850.040.13513620
CGTTCTG8700.040.086211
TCGTAAT403.4590812E-739.37517
TACGGGT700.038.571434
TAGTACG356.2494582E-638.571431
GTTTTTT205000.038.282932
CGAACCC1950.038.07692319
TCCGAAC1950.038.07692317
CGTTTTC7600.037.8947371
CCGAACT301.140074E-437.50000434
GCCGATG1500.037.59
TAGCCGT1750.037.28571344
CCGAACC2050.036.21951318
AACGAGC3750.036.015
TTGCCGA250.002107296136.036
Produced by FastQC (version 0.11.5)