##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936460.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1463050
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08790403608899	33.0	31.0	34.0	30.0	34.0
2	32.20364375790301	33.0	31.0	34.0	30.0	34.0
3	32.07566727042821	33.0	31.0	34.0	30.0	34.0
4	35.80642630122006	37.0	35.0	37.0	35.0	37.0
5	34.897339803834456	37.0	35.0	37.0	33.0	37.0
6	35.388739961040294	37.0	35.0	37.0	33.0	37.0
7	35.96167595092444	37.0	35.0	37.0	35.0	37.0
8	35.98634223027238	37.0	35.0	37.0	35.0	37.0
9	37.86024742831756	39.0	38.0	39.0	35.0	39.0
10	37.567381155804654	39.0	37.0	39.0	35.0	39.0
11	37.491331123338234	39.0	37.0	39.0	35.0	39.0
12	37.44576398619323	39.0	37.0	39.0	35.0	39.0
13	37.436057551006456	39.0	37.0	39.0	35.0	39.0
14	38.57081712860121	40.0	38.0	41.0	34.0	41.0
15	38.66917740336967	40.0	38.0	41.0	34.0	41.0
16	38.65471378285089	40.0	38.0	41.0	34.0	41.0
17	38.64224872697447	40.0	38.0	41.0	34.0	41.0
18	38.43961860496907	40.0	38.0	41.0	34.0	41.0
19	38.182420969891666	40.0	37.0	41.0	34.0	41.0
20	38.003353952359795	40.0	35.0	41.0	34.0	41.0
21	37.890522538532515	40.0	35.0	41.0	34.0	41.0
22	37.93631318136769	40.0	35.0	41.0	34.0	41.0
23	37.931402891220394	40.0	35.0	41.0	34.0	41.0
24	37.879556406137866	40.0	35.0	41.0	34.0	41.0
25	37.73129489764533	40.0	35.0	41.0	34.0	41.0
26	37.7518321315061	40.0	35.0	41.0	34.0	41.0
27	37.758531834182016	40.0	35.0	41.0	34.0	41.0
28	37.61989815795769	40.0	35.0	41.0	33.0	41.0
29	37.50816923550118	40.0	35.0	41.0	33.0	41.0
30	37.27274665937596	39.0	35.0	41.0	33.0	41.0
31	37.02968046204846	39.0	35.0	41.0	32.0	41.0
32	36.66879190731691	39.0	35.0	41.0	31.0	41.0
33	36.185752366631355	39.0	35.0	41.0	30.0	41.0
34	35.77185537062985	39.0	35.0	41.0	25.0	41.0
35	35.51801715594136	39.0	35.0	41.0	23.0	41.0
36	35.35165237004887	39.0	35.0	41.0	23.0	41.0
37	35.28550972283927	39.0	35.0	41.0	22.0	41.0
38	35.172138341136666	39.0	35.0	41.0	21.0	41.0
39	35.10386521308226	39.0	35.0	41.0	21.0	41.0
40	34.98905710672909	39.0	35.0	41.0	21.0	41.0
41	34.85075287925908	39.0	35.0	41.0	20.0	41.0
42	34.830651037216775	39.0	35.0	41.0	19.0	41.0
43	34.727862342366976	39.0	35.0	41.0	18.0	41.0
44	34.59374047366802	39.0	34.0	41.0	18.0	41.0
45	34.541521479101874	38.0	34.0	41.0	19.0	41.0
46	34.429717371245005	38.0	34.0	41.0	18.0	41.0
47	34.33821468849322	38.0	34.0	40.0	18.0	41.0
48	34.25437066402379	38.0	34.0	40.0	18.0	41.0
49	34.2157308362667	38.0	34.0	40.0	18.0	41.0
50	34.113499196883225	38.0	34.0	40.0	18.0	41.0
51	33.0743829670893	36.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	24.0
10	42.0
11	33.0
12	31.0
13	41.0
14	39.0
15	78.0
16	109.0
17	238.0
18	481.0
19	983.0
20	1648.0
21	2714.0
22	4290.0
23	6741.0
24	12059.0
25	21289.0
26	31719.0
27	35643.0
28	32740.0
29	28890.0
30	27665.0
31	29796.0
32	35011.0
33	46323.0
34	77487.0
35	93878.0
36	98967.0
37	146513.0
38	264376.0
39	462876.0
40	311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.554013875123886	19.236663135231193	21.522641058063634	23.686681931581287
2	42.45931444584942	20.608933392570318	21.61525580123714	15.316496360343118
3	27.807730426164518	20.82334848433068	36.09985988175387	15.26906120775093
4	24.59191415194286	22.616725334062405	35.82222070332525	16.969139810669493
5	21.864187826800176	27.592836881856396	33.7481972591504	16.794778032193022
6	23.408632650968865	29.037421824271213	33.52346126243122	14.030484262328697
7	70.43074399371176	4.99579645261611	19.503913058337037	5.069546495335088
8	72.20436758825741	4.04675164895253	19.089983254160828	4.6588975086292335
9	65.571716619391	6.395611906633403	21.577184648508254	6.455486825467345
10	33.501725846690135	25.215201120945967	27.296606404429102	13.986466627934794
11	24.297802535798503	22.610232049485663	35.99535217525033	17.0966132394655
12	21.973890160965105	20.48774819725915	38.398756023375824	19.13960561839992
13	21.09135026144014	20.176617340487336	40.52445234270872	18.207580055363795
14	18.118041078568744	22.973787635419157	40.089060524247294	18.819110761764808
15	17.641639041727895	23.906223300639077	40.48993540890605	17.962202248726975
16	20.932640716311816	23.899388264242507	38.01462697788865	17.153344041557023
17	20.724308806944396	24.359386213731586	36.36731485595161	18.54899012337241
18	21.245070229998973	24.018796350090565	36.462185161135984	18.273948258774478
19	20.559311028331226	26.076825809097432	34.80202317077338	18.561839991797957
20	20.77468302518711	26.49471993438365	34.37975462219337	18.350842418235878
21	20.615084925327228	25.72735039814087	36.50353713133522	17.15402754519668
22	20.50852670790472	22.502716926967636	37.068862991695426	19.919893373432213
23	18.911862205666242	24.172037866101636	36.84563070298349	20.070469225248626
24	19.681624004647823	23.610949728307304	36.99689005843956	19.710536208605312
25	21.469396124534363	24.59868083797546	34.293633163596596	19.638289873893576
26	20.16896209972318	24.965858993199138	34.41768907419432	20.44748983288336
27	20.57844913024162	23.457571511568297	35.06920474351526	20.894774614674823
28	20.083250743310206	23.839718396500462	35.03195379515396	21.045077065035372
29	21.472198489456957	23.2621578209904	34.89450121321896	20.371142476333688
30	23.456682956836744	22.11031748744062	35.19449096066437	19.238508595058267
31	23.101876217490858	23.085882232322888	34.52247018215372	19.289771368032536
32	22.879259082054613	23.28621714910632	34.782543317043164	19.051980451795906
33	22.09432350227265	24.669696866135812	33.58661699873552	19.649362632856022
34	20.342982126379823	25.5111581969174	34.042377225658726	20.10348245104405
35	20.26137179180479	26.676190150712554	32.40463415467688	20.65780390280578
36	20.895184716858616	27.879771709784357	30.707836369228662	20.51720720412836
37	20.060558422473598	26.89238235193602	31.544718225624553	21.502340999965824
38	19.708964150234102	27.38600868049622	31.69009944977957	21.214927719490106
39	20.16219541369058	25.48894432862855	30.876320016404087	23.472540241276786
40	21.890844468746796	25.27049656539421	31.86685349099484	20.97180547486415
41	20.386521308225966	25.614367246505587	31.66638187348348	22.33272957178497
42	20.72465055876423	26.18905710672909	31.730562865247258	21.355729469259423
43	20.851303783192645	24.85451625029903	32.665254092478044	21.62892587403028
44	21.978537985714773	24.64625269129558	31.27193192303749	22.103277399952155
45	21.006322408666826	24.018659649362633	31.40822254878507	23.56679539318547
46	21.57233177266669	24.443730562865248	30.76326851440484	23.220669150063227
47	20.662315026827518	24.527938211270975	32.4356652199173	22.37408154198421
48	20.212159529749496	24.522060079969926	32.64816650148662	22.617613888793958
49	20.951847168586173	23.747582105874717	32.398414271556	22.902156453983118
50	19.361265848740643	23.79091623662896	34.05392843716893	22.79388947746147
51	19.47718806602645	23.435631044735313	32.81220737500427	24.274973514233963
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	546.0
1	1020.0
2	1494.0
3	25210.0
4	48926.0
5	33241.0
6	17556.0
7	17074.0
8	16592.0
9	16407.5
10	16223.0
11	16013.5
12	15804.0
13	15064.0
14	14324.0
15	13503.0
16	12682.0
17	11955.0
18	11228.0
19	10673.0
20	10118.0
21	10114.0
22	10110.0
23	10074.0
24	10038.0
25	10983.5
26	13047.0
27	14165.0
28	16442.5
29	18720.0
30	20884.0
31	23048.0
32	26832.0
33	30616.0
34	34946.0
35	39276.0
36	42349.5
37	45423.0
38	49735.5
39	54048.0
40	57572.0
41	61096.0
42	66038.5
43	70981.0
44	77535.0
45	84089.0
46	104548.0
47	125007.0
48	131286.5
49	137566.0
50	133253.0
51	128940.0
52	112001.0
53	95062.0
54	84246.0
55	73430.0
56	66704.0
57	59978.0
58	55220.0
59	50462.0
60	45893.0
61	41324.0
62	37375.0
63	33426.0
64	28758.0
65	24090.0
66	21142.0
67	18194.0
68	15163.5
69	12133.0
70	10478.5
71	8824.0
72	7465.0
73	6106.0
74	5244.5
75	3352.5
76	2322.0
77	1889.0
78	1456.0
79	1074.5
80	693.0
81	497.0
82	301.0
83	228.0
84	155.0
85	127.0
86	99.0
87	66.5
88	34.0
89	26.0
90	18.0
91	12.5
92	7.0
93	6.0
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1463050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.411645634337226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.93594665335762	25.44257595874604
2	8.545686232044142	5.881422526386751
3	3.519521337395432	3.6333756319482076
4	2.1565661038423087	2.9684395420977934
5	1.5344244284369835	2.6401034842021964
6	1.1927546568246439	2.4626790347613094
7	1.003194386845005	2.4165098819726576
8	0.8508156246846285	2.3422372621443754
9	0.7504795153861554	2.3242711625367765
>10	6.390370406062841	40.41022280167642
>50	0.08599235689542653	2.0090120151995596
>100	0.029992596209416213	1.87527326879438
>500	0.0014185673384783794	0.3379409091007849
>1k	0.0020265247692548274	1.668574521678597
>5k	6.079574307764483E-4	1.6160632743503074
>10k+	2.0265247692548277E-4	1.9712987244038356
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28268	1.932128088582072	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTT	9976	0.6818632309217046	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTC	7496	0.5123543282867982	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCT	5702	0.38973377533235365	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG	4741	0.3240490755613274	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCT	3938	0.2691637332968798	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	2671	0.18256382215235295	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTC	2544	0.17388332592871056	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCC	2115	0.14456101978743036	No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGCT	2028	0.1386145381224155	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGT	1810	0.12371415877789549	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCG	1467	0.10026998393766447	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.101021837941287E-4	0.0	0.0	0.14708998325416084	0.0
2	4.101021837941287E-4	0.0	0.0	0.5059293940740235	0.0
3	4.101021837941287E-4	0.0	0.0	1.054099313078842	0.0
4	4.101021837941287E-4	0.0	0.0	1.6386316257134068	0.0
5	4.784525477598168E-4	0.0	0.0	3.0242302040258364	0.0
6	4.784525477598168E-4	0.0	0.0	4.993950992789037	0.0
7	5.46802911725505E-4	0.0	0.0	6.356378797717098	0.0
8	5.46802911725505E-4	0.0	0.0	7.9304876798468955	0.0
9	5.46802911725505E-4	0.0	0.0	8.955606438604285	0.0
10	5.46802911725505E-4	0.0	0.0	10.187348347629952	0.0
11	5.46802911725505E-4	0.0	0.0	11.30774751375551	0.0
12	5.46802911725505E-4	0.0	0.0	12.13109599808619	0.0
13	5.46802911725505E-4	0.0	0.0	12.59649362632856	0.0
14	6.151532756911931E-4	0.0	0.0	12.911247052390554	0.0
15	6.151532756911931E-4	0.0	0.0	13.172892245651209	0.0
16	6.151532756911931E-4	0.0	0.0	13.52523837189433	0.0
17	6.151532756911931E-4	0.0	0.0	13.930761081302759	0.0
18	6.151532756911931E-4	0.0	0.0	14.382762038207854	0.0
19	6.151532756911931E-4	0.0	0.0	14.688766617682239	0.0
20	6.835036396568812E-4	0.0	0.0	15.01418270052288	0.0
21	6.835036396568812E-4	0.0	0.0	15.385940330132257	0.0
22	6.835036396568812E-4	0.0	0.0	15.785789959331533	0.0
23	6.835036396568812E-4	0.0	0.0	16.190424114008408	0.0
24	6.835036396568812E-4	0.0	0.0	16.53737056149824	0.0
25	7.518540036225693E-4	0.0	0.0	16.85027852773316	0.0
26	7.518540036225693E-4	0.0	0.0	17.15211373500564	0.0
27	7.518540036225693E-4	0.0	0.0	17.50719387580739	0.0
28	8.202043675882574E-4	0.0	0.0	17.84969754963945	0.0
29	8.202043675882574E-4	0.0	0.0	18.211749427565703	0.0
30	8.202043675882574E-4	0.0	0.0	18.65691534807423	0.0
31	8.885547315539455E-4	0.0	0.0	19.019787430368066	0.0
32	8.885547315539455E-4	0.0	0.0	19.396397935819007	0.0
33	0.0010252554594853218	0.0	0.0	19.75475889409111	0.0
34	0.0010252554594853218	0.0	0.0	20.11428180855063	0.0
35	0.0010252554594853218	0.0	0.0	20.48679129216363	0.0
36	0.00109360582345101	0.0	0.0	20.831482177642595	0.0
37	0.00109360582345101	0.0	0.0	21.212740507843204	0.0
38	0.00109360582345101	0.0	0.0	21.63254844332046	0.0
39	0.001161956187416698	0.0	0.0	22.20969891664673	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	17830	0.0	44.0788	1
CGTTATT	490	0.0	41.785713	1
CGTTTCT	1115	0.0	41.367714	1
CCATACG	170	0.0	41.02941	2
TCACGAC	55	6.184564E-11	40.909092	25
GAACCCG	185	0.0	40.135136	20
CGTTCTG	870	0.0	40.08621	1
TCGTAAT	40	3.4590812E-7	39.375	17
TACGGGT	70	0.0	38.57143	4
TAGTACG	35	6.2494582E-6	38.57143	1
GTTTTTT	20500	0.0	38.28293	2
CGAACCC	195	0.0	38.076923	19
TCCGAAC	195	0.0	38.076923	17
CGTTTTC	760	0.0	37.894737	1
CCGAACT	30	1.140074E-4	37.500004	34
GCCGATG	150	0.0	37.5	9
TAGCCGT	175	0.0	37.285713	44
CCGAACC	205	0.0	36.219513	18
AACGAGC	375	0.0	36.0	15
TTGCCGA	25	0.0021072961	36.0	36
>>END_MODULE