FastQCFastQC Report
Sat 14 Jan 2017
SRR2936459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201226
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296052.464565369047956No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT101190.8423893588716862No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC80490.6700654164994764No Hit
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG55940.4656908858116624No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT54120.45053969860792226No Hit
CGTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT30190.2513265613631407No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC24640.20512376521986705No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC23530.1958832059912123No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC19740.16433210736364348No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGT17920.1491809201599033No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG14070.11713033184429908No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT13950.11613135246822828Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA207.033413E-445.00000428
CGTTTTT159500.044.1677131
CGTTTCT12400.041.5524181
CGTTATT4500.041.51
CGTTCTG8700.041.120691
TCACGAC556.184564E-1140.90909225
GTTTTTT178650.039.2191472
CGTTTTC6600.039.2045441
TACGAAT1100.038.86363612
TAGCCGT1450.038.79310644
CGTAAGG1400.038.5714262
TATAGCG301.1399524E-437.5000041
TAGTACG301.1399524E-437.5000041
CACGACC1650.036.8181827
AACACGT1350.036.66666841
TCGAGTA250.002107146736.023
TAGGACG250.002107146736.01
GCTAACG250.002107146736.01
ACGACCA1700.035.73529428
TAACGCC458.7003536E-735.00000412
Produced by FastQC (version 0.11.5)