##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936459.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1201226
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07296711859384	33.0	31.0	34.0	30.0	34.0
2	32.19960939906396	33.0	31.0	34.0	30.0	34.0
3	32.05807233609662	33.0	31.0	34.0	30.0	34.0
4	35.79577198628734	37.0	35.0	37.0	35.0	37.0
5	34.97452103101332	37.0	35.0	37.0	33.0	37.0
6	35.41816693944354	37.0	35.0	37.0	33.0	37.0
7	35.9581968755255	37.0	35.0	37.0	35.0	37.0
8	35.96789863023278	37.0	35.0	37.0	35.0	37.0
9	37.82719654752728	39.0	38.0	39.0	35.0	39.0
10	37.56897619598644	39.0	37.0	39.0	35.0	39.0
11	37.48401300005161	39.0	37.0	39.0	35.0	39.0
12	37.32274359695844	39.0	37.0	39.0	35.0	39.0
13	37.261225614497185	39.0	37.0	39.0	34.0	39.0
14	38.34476859475236	40.0	38.0	41.0	34.0	41.0
15	38.455625336114934	40.0	38.0	41.0	34.0	41.0
16	38.439460184844485	40.0	38.0	41.0	34.0	41.0
17	38.40663289006398	40.0	38.0	41.0	34.0	41.0
18	38.17026104996062	39.0	37.0	41.0	34.0	41.0
19	37.91259263452506	39.0	37.0	41.0	34.0	41.0
20	37.72572438492007	39.0	35.0	41.0	34.0	41.0
21	37.615457873872195	39.0	35.0	41.0	33.0	41.0
22	37.6674747299842	39.0	35.0	41.0	34.0	41.0
23	37.66948101356447	39.0	35.0	41.0	34.0	41.0
24	37.61223866283281	39.0	35.0	41.0	34.0	41.0
25	37.456575199005016	39.0	35.0	41.0	33.0	41.0
26	37.46741079530413	39.0	35.0	41.0	33.0	41.0
27	37.47155156481794	39.0	35.0	41.0	34.0	41.0
28	37.32911458792933	39.0	35.0	41.0	33.0	41.0
29	37.221457078018624	39.0	35.0	41.0	33.0	41.0
30	36.97107122223462	39.0	35.0	41.0	33.0	41.0
31	36.72176426417676	39.0	35.0	41.0	32.0	41.0
32	36.361002842096326	39.0	35.0	41.0	31.0	41.0
33	35.8534422331851	39.0	35.0	41.0	29.0	41.0
34	35.390171374911965	39.0	35.0	41.0	24.0	41.0
35	35.08614115911577	38.0	35.0	41.0	23.0	41.0
36	34.92931804672892	38.0	35.0	41.0	22.0	41.0
37	34.827959934267156	38.0	35.0	41.0	21.0	41.0
38	34.671912695862396	38.0	34.0	41.0	21.0	41.0
39	34.56905944426777	38.0	34.0	40.0	20.0	41.0
40	34.432873580824925	38.0	34.0	40.0	19.0	41.0
41	34.27588064194415	38.0	34.0	40.0	18.0	41.0
42	34.24805074149244	38.0	34.0	40.0	18.0	41.0
43	34.14844667031849	38.0	34.0	40.0	18.0	41.0
44	34.01685944193682	37.0	34.0	40.0	18.0	41.0
45	33.96700537617401	37.0	34.0	40.0	18.0	41.0
46	33.871768509839114	37.0	33.0	40.0	18.0	41.0
47	33.78711832744213	37.0	33.0	40.0	18.0	41.0
48	33.71440095369231	37.0	33.0	40.0	18.0	41.0
49	33.669855630830504	36.0	33.0	40.0	18.0	41.0
50	33.52871149975109	36.0	33.0	40.0	18.0	41.0
51	32.47293348628818	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	18.0
10	25.0
11	18.0
12	31.0
13	27.0
14	43.0
15	69.0
16	130.0
17	248.0
18	449.0
19	935.0
20	1544.0
21	2386.0
22	3614.0
23	5544.0
24	9470.0
25	16813.0
26	26163.0
27	30939.0
28	29779.0
29	26738.0
30	25440.0
31	27467.0
32	32599.0
33	43646.0
34	81152.0
35	92991.0
36	87198.0
37	122764.0
38	209458.0
39	323279.0
40	239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.57761154020975	18.86023113052831	21.144896963602186	26.41726036565975
2	43.01863263032934	20.541763165299454	21.465652591602247	14.973951612768953
3	27.14776403441151	20.433207406433095	37.43025875230806	14.988769806847339
4	24.117276848819454	22.050804761135705	37.461643354372946	16.370275035671888
5	21.854921555144493	26.60714969539454	35.020803745506676	16.517125003954295
6	22.80470119694379	28.310076538469865	34.57625792315518	14.308964341431171
7	69.28171717894884	4.899327853376468	20.56590516688783	5.2530498007868625
8	71.10152460902445	3.855810646789197	20.413977053443734	4.628687690742624
9	65.36613426615807	5.313821046164502	22.41326777808672	6.906776909590702
10	36.68152370994301	20.40099032155481	28.347371768509838	14.57011419999234
11	28.030029320044687	21.770258053022495	33.494196762307844	16.705515864624974
12	25.427771293661642	19.30119727678222	36.71865244342031	18.55237898613583
13	22.85997805575304	18.523575080792458	39.50688713031519	19.10955973313931
14	18.215889432962655	20.979565876862473	40.55331802674934	20.251226663425534
15	18.265505408640838	21.231974665883023	41.503097668548634	18.999422256927506
16	22.64602997271121	22.175344190019196	37.7465189731158	17.43210686415379
17	22.245106249781475	22.885452029842842	36.18294975300235	18.686491967373335
18	22.79462815490174	22.81335901820307	36.47490147565903	17.917111351236155
19	21.574957585000657	25.652125411870873	34.367387985275045	18.405529017853425
20	22.33334942800106	25.689836883317547	33.580858223181984	18.395955465499412
21	21.73837396126957	25.713063153811188	35.55808815327007	16.990474731649165
22	21.211995078361607	22.36231982990711	36.68160695822435	19.744078133506935
23	20.192869618206732	22.800538782876828	36.7929099103749	20.21368168854154
24	21.59268946892591	21.654043452272926	36.90854177315509	19.844725305646065
25	23.184063615006668	22.68948557557029	33.757094834777135	20.369355974645902
26	20.502719721351355	22.516662143510047	34.633699237279245	22.346918897859354
27	20.856941158449786	21.543739479498445	35.50372702555556	22.095592336496214
28	20.70476330016167	23.451956584356314	34.08467682184701	21.758603293635005
29	22.800622031158166	23.73475099606569	32.83112420144086	20.633502771335284
30	25.149056047737893	22.167685348135986	33.94498620575978	18.738272398366334
31	25.220649569689634	23.38003006927922	32.86875242460619	18.53056793642495
32	25.77957853060124	24.218756503771978	31.495072534227532	18.506592431399255
33	25.618742851053838	25.54648334285139	30.41342761478689	18.42134619130788
34	23.268976861972686	26.230367974053177	32.73314097430459	17.767514189669555
35	23.029554804841055	27.116795673753312	32.25030094253704	17.603348578868587
36	24.400737246779542	29.238627868527654	28.711000261399604	17.649634623293203
37	24.79949651439446	28.127513057492926	29.349930820678203	17.723059607434404
38	24.328477738577085	27.32658134272818	29.942741832094878	18.402199086599857
39	23.972341591007854	25.51143581640757	29.59534675406626	20.920875838518313
40	25.827696037215315	23.210869561597903	31.36370674627422	19.597727654912564
41	23.06485207612889	24.36951914127733	31.036041510923006	21.529587271670778
42	22.62272045393623	25.204915644516518	30.702215902752688	21.470147998794566
43	22.255762029792894	23.704698366502225	31.74448438511987	22.29505521858501
44	23.24033945319199	23.22485527286289	30.431742236681526	23.103063037263595
45	22.17409546579911	23.260319040713405	30.3712207361479	24.194364757339585
46	22.499679494116844	23.925472808613865	30.321022022500344	23.253825674768944
47	21.67110934994747	24.16864103840576	32.08771704908152	22.072532562565243
48	21.01786008627852	23.517223236926274	33.04715349151617	22.417763185279043
49	22.293223756395548	22.506006363498628	32.285764710387554	22.91500516971827
50	20.516372439490986	22.778144995196577	33.99110575362172	22.714376811690723
51	20.383008692785538	22.175593934863215	33.39421557641942	24.047181795931824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	586.0
1	991.5
2	1397.0
3	25571.5
4	49746.0
5	32654.0
6	15562.0
7	15115.0
8	14668.0
9	14590.5
10	14513.0
11	14110.5
12	13708.0
13	12834.5
14	11961.0
15	11199.5
16	10438.0
17	9637.5
18	8837.0
19	8470.5
20	8104.0
21	7656.0
22	7208.0
23	7121.0
24	7034.0
25	7387.0
26	8791.0
27	9842.0
28	10964.5
29	12087.0
30	13415.5
31	14744.0
32	17238.0
33	19732.0
34	22887.5
35	26043.0
36	28068.5
37	30094.0
38	32158.5
39	34223.0
40	36534.5
41	38846.0
42	42151.5
43	45457.0
44	51406.0
45	57355.0
46	74167.5
47	90980.0
48	97119.0
49	103258.0
50	102104.5
51	100951.0
52	89768.0
53	78585.0
54	71367.5
55	64150.0
56	61282.5
57	58415.0
58	56551.5
59	54688.0
60	52684.0
61	50680.0
62	46275.5
63	41871.0
64	36840.0
65	31809.0
66	27153.5
67	22498.0
68	18821.0
69	15144.0
70	12876.0
71	10608.0
72	8934.0
73	7260.0
74	6035.0
75	3752.0
76	2694.0
77	2108.5
78	1523.0
79	1119.0
80	715.0
81	555.0
82	395.0
83	278.0
84	161.0
85	105.0
86	49.0
87	31.5
88	14.0
89	13.5
90	13.0
91	13.5
92	14.0
93	9.5
94	5.0
95	7.5
96	10.0
97	5.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1201226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.14393396064653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.71891245222334	25.9220363540255
2	8.51735207677	6.157012219647008
3	3.9498449078511673	4.282888005125057
4	2.4744745811570907	3.5774898339456143
5	1.9622217900820094	3.5461207398432877
6	1.5710587285327555	3.407054575943113
7	1.3867100680275146	3.50848099849451
8	1.1942243300627344	3.4531172255987848
9	1.0707508054446835	3.4831031760271545
>10	6.061510015132445	32.698568265128074
>50	0.061573911998412584	1.5611082266940894
>100	0.026918868029386415	1.7450559984030372
>500	0.0016385396220792395	0.46634093231792734
>1k	0.0016385396220792395	1.2186456438874138
>5k	7.022312666053884E-4	1.6121419566977695
>10k+	4.681541777369256E-4	3.3608358482215794
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29605	2.464565369047956	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT	10119	0.8423893588716862	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC	8049	0.6700654164994764	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	5594	0.4656908858116624	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	5412	0.45053969860792226	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	3019	0.2513265613631407	No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	2464	0.20512376521986705	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC	2353	0.1958832059912123	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC	1974	0.16433210736364348	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGT	1792	0.1491809201599033	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG	1407	0.11713033184429908	No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT	1395	0.11613135246822828	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1624140669615877E-4	0.0	0.0	0.1229577115380453	0.0
2	4.1624140669615877E-4	0.0	0.0	0.4642756650288955	0.0
3	4.1624140669615877E-4	0.0	0.0	1.136588785124531	0.0
4	4.1624140669615877E-4	0.0	0.0	1.6574732814641042	0.0
5	4.1624140669615877E-4	0.0	0.0	3.0807691475209493	0.0
6	4.1624140669615877E-4	0.0	0.0	5.149155945675502	0.0
7	4.994896880353905E-4	0.0	0.0	6.5692051287601165	0.0
8	4.994896880353905E-4	0.0	0.0	8.138018990597939	0.0
9	4.994896880353905E-4	0.0	0.0	9.326221710152794	0.0
10	4.994896880353905E-4	0.0	0.0	10.715552277423233	0.0
11	4.994896880353905E-4	0.0	0.0	11.85996640099365	0.0
12	4.994896880353905E-4	0.0	0.0	12.637921590108773	0.0
13	4.994896880353905E-4	0.0	0.0	13.08904402668607	0.0
14	4.994896880353905E-4	0.0	0.0	13.406053482025863	0.0
15	4.994896880353905E-4	0.0	0.0	13.655132339792845	0.0
16	4.994896880353905E-4	0.0	0.0	13.955075897458096	0.0
17	4.994896880353905E-4	0.0	0.0	14.268089435293609	0.0
18	4.994896880353905E-4	0.0	0.0	14.636546328501048	0.0
19	5.827379693746223E-4	0.0	0.0	14.883543979234549	0.0
20	6.65986250713854E-4	0.0	0.0	15.154933376400445	0.0
21	6.65986250713854E-4	0.0	0.0	15.438643519204547	0.0
22	6.65986250713854E-4	0.0	0.0	15.779961472695396	0.0
23	6.65986250713854E-4	0.0	0.0	16.135764627139274	0.0
24	6.65986250713854E-4	0.0	0.0	16.433626977771045	0.0
25	6.65986250713854E-4	0.0	0.0	16.69752402961641	0.0
26	6.65986250713854E-4	0.0	0.0	16.972409854598553	0.0
27	6.65986250713854E-4	0.0	0.0	17.262446866784437	0.0
28	7.492345320530857E-4	0.0	0.0	17.52842512566328	0.0
29	7.492345320530857E-4	0.0	0.0	17.81463271690756	0.0
30	7.492345320530857E-4	0.0	0.0	18.225629481879345	0.0
31	8.324828133923175E-4	0.0	0.0	18.51117108687291	0.0
32	8.324828133923175E-4	0.0	0.0	18.8266820731486	0.0
33	8.324828133923175E-4	0.0	0.0	19.136282431449203	0.0
34	8.324828133923175E-4	0.0	0.0	19.445549796624448	0.0
35	9.157310947315492E-4	0.0	0.0	19.77163331463022	0.0
36	9.157310947315492E-4	0.0	0.0	20.101712750140273	0.0
37	9.157310947315492E-4	0.0	0.0	20.457182911458794	0.0
38	9.157310947315492E-4	0.0	0.0	20.942187398541158	0.0
39	9.157310947315492E-4	0.0	0.0	21.57911999906762	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCTA	20	7.033413E-4	45.000004	28
CGTTTTT	15950	0.0	44.167713	1
CGTTTCT	1240	0.0	41.552418	1
CGTTATT	450	0.0	41.5	1
CGTTCTG	870	0.0	41.12069	1
TCACGAC	55	6.184564E-11	40.909092	25
GTTTTTT	17865	0.0	39.219147	2
CGTTTTC	660	0.0	39.204544	1
TACGAAT	110	0.0	38.863636	12
TAGCCGT	145	0.0	38.793106	44
CGTAAGG	140	0.0	38.571426	2
TATAGCG	30	1.1399524E-4	37.500004	1
TAGTACG	30	1.1399524E-4	37.500004	1
CACGACC	165	0.0	36.81818	27
AACACGT	135	0.0	36.666668	41
TCGAGTA	25	0.0021071467	36.0	23
TAGGACG	25	0.0021071467	36.0	1
GCTAACG	25	0.0021071467	36.0	1
ACGACCA	170	0.0	35.735294	28
TAACGCC	45	8.7003536E-7	35.000004	12
>>END_MODULE