##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936457.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 775387 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67790148661249 31.0 31.0 34.0 30.0 34.0 2 31.725734375221663 31.0 31.0 34.0 30.0 34.0 3 31.78386276788236 31.0 31.0 34.0 30.0 34.0 4 35.51769503486646 37.0 35.0 37.0 33.0 37.0 5 34.54257293454752 37.0 35.0 37.0 32.0 37.0 6 34.99262045920295 37.0 35.0 37.0 32.0 37.0 7 35.52881077449067 37.0 35.0 37.0 33.0 37.0 8 35.37486055350425 37.0 35.0 37.0 33.0 37.0 9 37.29947755120991 39.0 37.0 39.0 34.0 39.0 10 36.90068830145463 39.0 37.0 39.0 33.0 39.0 11 36.88750907611296 39.0 37.0 39.0 33.0 39.0 12 36.85077903034227 39.0 35.0 39.0 33.0 39.0 13 36.659375253905466 39.0 35.0 39.0 32.0 39.0 14 37.76440409756676 40.0 36.0 41.0 33.0 41.0 15 37.60939762982872 40.0 36.0 41.0 32.0 41.0 16 37.885368209681104 40.0 36.0 41.0 33.0 41.0 17 37.65467953421969 39.0 36.0 41.0 32.0 41.0 18 37.65078728428514 39.0 36.0 41.0 32.0 41.0 19 37.568922357480844 39.0 36.0 41.0 32.0 41.0 20 37.32304900649611 39.0 35.0 41.0 32.0 41.0 21 37.57749227160115 39.0 36.0 41.0 32.0 41.0 22 37.60158088799528 39.0 36.0 41.0 33.0 41.0 23 37.54527610083739 39.0 36.0 41.0 33.0 41.0 24 37.4560677442361 39.0 36.0 41.0 32.0 41.0 25 37.51747063079469 39.0 36.0 41.0 33.0 41.0 26 37.41413255574313 39.0 36.0 41.0 32.0 41.0 27 37.39999509922142 39.0 36.0 41.0 32.0 41.0 28 37.26711693644593 39.0 36.0 41.0 32.0 41.0 29 37.29473282373834 39.0 36.0 41.0 32.0 41.0 30 37.23981057201114 39.0 36.0 41.0 32.0 41.0 31 37.17097913686972 39.0 36.0 41.0 31.0 41.0 32 37.009009694513836 39.0 36.0 41.0 31.0 41.0 33 36.84058541089804 39.0 35.0 41.0 31.0 41.0 34 36.7390025883849 39.0 35.0 41.0 30.0 41.0 35 36.62253171642032 39.0 35.0 41.0 30.0 41.0 36 36.57588791145583 39.0 35.0 41.0 30.0 41.0 37 36.4583427372396 39.0 35.0 41.0 30.0 41.0 38 36.33100761297262 39.0 35.0 41.0 30.0 41.0 39 36.35681408122654 39.0 35.0 41.0 30.0 41.0 40 36.15088465501743 39.0 35.0 40.0 30.0 41.0 41 36.01932325406539 39.0 35.0 40.0 29.0 41.0 42 36.15884713052966 39.0 35.0 40.0 30.0 41.0 43 36.15679267256222 39.0 35.0 40.0 30.0 41.0 44 36.11231165856534 39.0 35.0 40.0 30.0 41.0 45 36.16099960406868 39.0 35.0 40.0 29.0 41.0 46 36.01927811531532 39.0 35.0 40.0 28.0 41.0 47 35.667028206560076 38.0 35.0 40.0 27.0 41.0 48 35.69233685888466 38.0 35.0 40.0 27.0 41.0 49 35.801441086837926 39.0 35.0 40.0 28.0 41.0 50 35.652880432609784 38.0 35.0 40.0 27.0 41.0 51 34.39304760074646 37.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 13.0 10 8.0 11 9.0 12 12.0 13 9.0 14 10.0 15 21.0 16 42.0 17 86.0 18 217.0 19 701.0 20 1537.0 21 2725.0 22 3772.0 23 4705.0 24 5482.0 25 6568.0 26 7590.0 27 8206.0 28 8769.0 29 10305.0 30 13467.0 31 17821.0 32 24395.0 33 32755.0 34 48604.0 35 62263.0 36 60406.0 37 86877.0 38 150644.0 39 217253.0 40 109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.17122159644152 18.28235448879076 21.57645150099241 8.969972413775315 2 25.759394985987644 39.573142185773044 20.193658134583117 14.4738046936562 3 23.60924286839991 39.23021665310355 23.662506593481705 13.498033885014838 4 22.660942213372163 19.825841805446828 42.25025696845575 15.262959012725258 5 33.299500765424234 25.538215110647972 23.054939017548655 18.107345106379135 6 19.894065801980172 41.81370077135676 25.457352264095217 12.834881162567854 7 66.01864617281436 4.721642225108236 24.348744562392717 4.910967039684699 8 62.99551062888597 17.055999133336 12.87640881263163 7.07208142514641 9 59.57863621649576 5.137563565032687 12.55656852642616 22.72723169204539 10 39.10382815290945 17.53975756622177 24.648852766425026 18.707561514443753 11 32.0202685884597 22.05608296244327 24.809546716671804 21.114101732425226 12 23.23433330710987 18.416481060425312 31.26451694444194 27.084668688022884 13 22.95666551025488 22.454077770197333 38.972796809851076 15.616459909696706 14 21.06780227163984 31.339576237414352 25.852122875415763 21.74049861553005 15 15.912183206579424 23.604342089820953 41.22831566688634 19.25515903671328 16 16.95888633675829 25.717609400209184 26.60348961228393 30.720014650748595 17 18.111472077814046 26.91804221633842 31.734991688021598 23.235494017825935 18 18.02738503482777 25.68317498229916 31.39283996249615 24.896600020376923 19 17.388091365988856 28.66852294402666 27.513228877966746 26.430156812017742 20 20.399748770613897 27.175977931020252 34.820676642760326 17.603596655605525 21 18.891985550441266 34.40191800997437 28.882093715783213 17.824002723801147 22 16.403937646620335 22.933322328076173 32.20211326731039 28.460626757993108 23 17.929756366820698 31.429982705410332 29.66802383841875 20.97223708935022 24 21.830002308524644 24.83572719171201 26.525979930022043 26.808290569741306 25 15.626519402569297 37.14119529989541 24.682771312905686 22.54951398462961 26 16.48402668602904 24.127822622767727 31.475508359051673 27.91264233215156 27 22.551706438204405 27.625817817425364 26.38334147980299 23.43913426456724 28 14.13294264670416 26.701376216005684 34.44666985647167 24.71901128081848 29 19.26766891887535 21.776351679870825 29.426338073761876 29.52964132749195 30 17.082824447662908 30.87155188312417 33.261197311793985 18.78442635741894 31 21.76409973342344 27.765876910497596 23.746722604325324 26.72330075175364 32 21.59347525816141 28.720883894107075 28.253246443388914 21.432394404342606 33 20.120275423756137 28.352809629256097 24.118794872753863 27.4081200742339 34 24.911689259685808 23.93114664032283 24.788395988067894 26.368768111923462 35 18.806479861024236 22.306280605684645 28.769633744181938 30.11760578910918 36 23.58706039693727 28.346619172103736 23.318291382238804 24.748029048720188 37 17.702128098613983 22.763858563530214 38.81042627745887 20.723587060396937 38 17.741205359388278 33.03279523644322 23.541405775438587 25.684593628729914 39 26.425127065581442 22.889731192294942 30.59826899341877 20.086872748704838 40 18.48277053909854 26.424869129866764 28.907629351536716 26.184730979497978 41 26.663846569519478 25.043752345602904 22.58175594896484 25.710645135912774 42 19.532053026424222 20.92735627499558 32.165099492253546 27.375491206326647 43 27.13909312382075 22.095934030361615 25.318969753168417 25.446003092649217 44 21.259319539791097 22.401974755831606 30.56112625050459 25.77757945387271 45 18.645656942920116 21.46257288296038 27.954041014357994 31.937729159761513 46 28.712114079807886 25.122422738580863 25.411439706881854 20.754023474729394 47 16.50491947891827 22.428929038015855 38.89309467401439 22.17305680905148 48 22.036866751699474 24.75950718802353 26.158421536600436 27.045204523676563 49 19.66256849805323 18.750894714510302 37.11875489271809 24.467781894718378 50 23.095950796183068 19.16243114728516 30.810163183029893 26.931454873501874 51 20.293737191879668 19.745881733895462 26.024681868537904 33.935699205686966 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 272.0 1 368.0 2 464.0 3 1969.0 4 3474.0 5 2483.5 6 1493.0 7 1536.5 8 1580.0 9 1561.0 10 1542.0 11 1611.0 12 1680.0 13 1702.0 14 1724.0 15 1701.0 16 1678.0 17 1639.0 18 1600.0 19 1559.0 20 1518.0 21 1653.5 22 1789.0 23 1934.0 24 2079.0 25 2493.0 26 3289.0 27 3671.0 28 4464.5 29 5258.0 30 6498.5 31 7739.0 32 9157.0 33 10575.0 34 12381.5 35 14188.0 36 16450.5 37 18713.0 38 21410.0 39 24107.0 40 27885.0 41 31663.0 42 36563.0 43 41463.0 44 50662.5 45 59862.0 46 124044.5 47 188227.0 48 142414.5 49 96602.0 50 91754.0 51 86906.0 52 69991.0 53 53076.0 54 44283.0 55 35490.0 56 30425.5 57 25361.0 58 21815.5 59 18270.0 60 14809.0 61 11348.0 62 9855.0 63 8362.0 64 6544.0 65 4726.0 66 3786.0 67 2846.0 68 2066.5 69 1287.0 70 1118.0 71 949.0 72 694.5 73 440.0 74 336.0 75 183.0 76 134.0 77 91.0 78 48.0 79 32.0 80 16.0 81 16.0 82 16.0 83 9.0 84 2.0 85 4.0 86 6.0 87 4.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 1.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 775387.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.07361655198604 #Duplication Level Percentage of deduplicated Percentage of total 1 73.80776031261458 24.41089563140308 2 11.708492769573093 7.744844005251229 3 4.303225821788797 4.269697222993432 4 2.131641001822118 2.8200430848302442 5 1.2999439521950058 2.149692390698544 6 0.882188028762461 1.7506289154025239 7 0.6527995016396525 1.5113308281790214 8 0.5515659935518828 1.459382573908014 9 0.4512567884154631 1.3432224587880348 >10 3.961935889684521 26.34270763591733 >50 0.185617073579697 3.9132733742312777 >100 0.05219253844184909 3.049915780568318 >500 0.0039242510106653455 0.8490807709467609 >1k 0.0047091012127984146 3.4160510381104783 >5k 0.001569700404266138 3.44836854223242 >10k+ 0.0011772753031996036 11.520865746539313 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC 31135 4.015414238309386 No Hit GAATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTC 29246 3.771793955792398 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG 28385 3.6607526306218703 No Hit GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC 9037 1.165482526789848 No Hit GAATGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCT 6421 0.8281026119860146 No Hit GAACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT 5980 0.7712277868986712 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTA 5131 0.6617340760162345 No Hit GAATGATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTC 4859 0.6266548188195056 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATAGCGT 4768 0.6149187438014824 No Hit CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC 2669 0.34421521124290194 No Hit CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT 2538 0.32732042193124206 No Hit GAATGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTT 2007 0.2588384896832162 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1764 0.2274993003493739 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATG 1529 0.19719185387425892 No Hit TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC 1483 0.19125933243657683 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGT 1298 0.16740027882850755 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTAT 1251 0.16133878953348457 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC 1098 0.1416067073603246 No Hit ACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC 1056 0.13619005735200615 No Hit GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTATAGCG 993 0.1280650823395285 No Hit CGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG 832 0.10730125730764122 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.579357146818299E-4 0.0 0.0 0.3466656005323793 0.0 2 2.579357146818299E-4 0.0 0.0 2.2895663713732626 0.0 3 2.579357146818299E-4 0.0 0.0 2.9178977723382 0.0 4 2.579357146818299E-4 0.0 0.0 4.395869417465085 0.0 5 2.579357146818299E-4 0.0 0.0 9.900088600917993 0.0 6 2.579357146818299E-4 0.0 0.0 11.1499161064088 0.0 7 2.579357146818299E-4 0.0 0.0 12.936120930580472 0.0 8 2.579357146818299E-4 0.0 0.0 15.020112537352317 0.0 9 2.579357146818299E-4 0.0 0.0 15.530309380992975 0.0 10 3.869035720227448E-4 0.0 0.0 20.65484719243423 0.0 11 3.869035720227448E-4 0.0 0.0 22.458720613061608 0.0 12 3.869035720227448E-4 0.0 0.0 28.130726978915046 0.0 13 3.869035720227448E-4 0.0 0.0 28.93819473372651 0.0 14 3.869035720227448E-4 0.0 0.0 29.343024837919646 0.0 15 3.869035720227448E-4 0.0 0.0 30.438735753888057 0.0 16 3.869035720227448E-4 0.0 0.0 31.487115466212355 0.0 17 3.869035720227448E-4 0.0 0.0 32.65079244299943 0.0 18 3.869035720227448E-4 0.0 0.0 33.86167165557328 0.0 19 5.158714293636597E-4 0.0 0.0 35.355119443581074 0.0 20 5.158714293636597E-4 0.0 0.0 36.21546401990232 0.0 21 5.158714293636597E-4 0.0 0.0 37.10173113554909 0.0 22 5.158714293636597E-4 0.0 0.0 38.17087467290527 0.0 23 5.158714293636597E-4 0.0 0.0 39.1421316065397 0.0 24 5.158714293636597E-4 0.0 0.0 39.90497648271121 0.0 25 6.448392867045746E-4 0.0 0.0 40.56709746229947 0.0 26 6.448392867045746E-4 0.0 0.0 41.19143085968684 0.0 27 6.448392867045746E-4 0.0 0.0 41.90978182507574 0.0 28 7.738071440454896E-4 0.0 0.0 42.53282554388969 0.0 29 7.738071440454896E-4 0.0 0.0 43.1992024627702 0.0 30 7.738071440454896E-4 0.0 0.0 43.9125236817228 0.0 31 7.738071440454896E-4 0.0 0.0 44.59450571134156 0.0 32 7.738071440454896E-4 0.0 0.0 45.23122002303366 0.0 33 7.738071440454896E-4 0.0 0.0 45.835305466818504 0.0 34 7.738071440454896E-4 0.0 0.0 46.40701997841078 0.0 35 9.027750013864044E-4 0.0 0.0 47.01265303648372 0.0 36 9.027750013864044E-4 0.0 0.0 47.59042903737102 0.0 37 0.0010317428587273195 0.0 0.0 48.15762967395636 0.0 38 0.0011607107160682343 0.0 0.0 48.73669535341707 0.0 39 0.0011607107160682343 0.0 0.0 49.32736814003846 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATTA 20 7.031864E-4 45.000004 20 TAGCGCG 20 7.031864E-4 45.000004 1 ACGATTG 30 2.1645847E-6 45.000004 1 TTCCCGT 20 7.031864E-4 45.000004 29 CCCCGGA 20 7.031864E-4 45.000004 25 AGACCGA 35 1.2112469E-7 45.000004 44 GTCCGTC 20 7.031864E-4 45.000004 9 CGCAGCG 30 2.1645847E-6 45.000004 31 TGCTCGA 20 7.031864E-4 45.000004 16 TTACGCG 20 7.031864E-4 45.000004 1 TTACGAC 20 7.031864E-4 45.000004 19 ACCGTAT 20 7.031864E-4 45.000004 18 CACCGTA 20 7.031864E-4 45.000004 17 CGGTACC 40 6.8102963E-9 45.000004 14 CCCGGTA 40 6.8102963E-9 45.000004 12 CCCGGAG 20 7.031864E-4 45.000004 26 GCGTAAG 20 7.031864E-4 45.000004 1 GCATCGA 20 7.031864E-4 45.000004 9 CTTACCG 20 7.031864E-4 45.000004 1 AACGTAG 45 3.8380676E-10 45.0 1 >>END_MODULE