##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936457.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775387
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67790148661249	31.0	31.0	34.0	30.0	34.0
2	31.725734375221663	31.0	31.0	34.0	30.0	34.0
3	31.78386276788236	31.0	31.0	34.0	30.0	34.0
4	35.51769503486646	37.0	35.0	37.0	33.0	37.0
5	34.54257293454752	37.0	35.0	37.0	32.0	37.0
6	34.99262045920295	37.0	35.0	37.0	32.0	37.0
7	35.52881077449067	37.0	35.0	37.0	33.0	37.0
8	35.37486055350425	37.0	35.0	37.0	33.0	37.0
9	37.29947755120991	39.0	37.0	39.0	34.0	39.0
10	36.90068830145463	39.0	37.0	39.0	33.0	39.0
11	36.88750907611296	39.0	37.0	39.0	33.0	39.0
12	36.85077903034227	39.0	35.0	39.0	33.0	39.0
13	36.659375253905466	39.0	35.0	39.0	32.0	39.0
14	37.76440409756676	40.0	36.0	41.0	33.0	41.0
15	37.60939762982872	40.0	36.0	41.0	32.0	41.0
16	37.885368209681104	40.0	36.0	41.0	33.0	41.0
17	37.65467953421969	39.0	36.0	41.0	32.0	41.0
18	37.65078728428514	39.0	36.0	41.0	32.0	41.0
19	37.568922357480844	39.0	36.0	41.0	32.0	41.0
20	37.32304900649611	39.0	35.0	41.0	32.0	41.0
21	37.57749227160115	39.0	36.0	41.0	32.0	41.0
22	37.60158088799528	39.0	36.0	41.0	33.0	41.0
23	37.54527610083739	39.0	36.0	41.0	33.0	41.0
24	37.4560677442361	39.0	36.0	41.0	32.0	41.0
25	37.51747063079469	39.0	36.0	41.0	33.0	41.0
26	37.41413255574313	39.0	36.0	41.0	32.0	41.0
27	37.39999509922142	39.0	36.0	41.0	32.0	41.0
28	37.26711693644593	39.0	36.0	41.0	32.0	41.0
29	37.29473282373834	39.0	36.0	41.0	32.0	41.0
30	37.23981057201114	39.0	36.0	41.0	32.0	41.0
31	37.17097913686972	39.0	36.0	41.0	31.0	41.0
32	37.009009694513836	39.0	36.0	41.0	31.0	41.0
33	36.84058541089804	39.0	35.0	41.0	31.0	41.0
34	36.7390025883849	39.0	35.0	41.0	30.0	41.0
35	36.62253171642032	39.0	35.0	41.0	30.0	41.0
36	36.57588791145583	39.0	35.0	41.0	30.0	41.0
37	36.4583427372396	39.0	35.0	41.0	30.0	41.0
38	36.33100761297262	39.0	35.0	41.0	30.0	41.0
39	36.35681408122654	39.0	35.0	41.0	30.0	41.0
40	36.15088465501743	39.0	35.0	40.0	30.0	41.0
41	36.01932325406539	39.0	35.0	40.0	29.0	41.0
42	36.15884713052966	39.0	35.0	40.0	30.0	41.0
43	36.15679267256222	39.0	35.0	40.0	30.0	41.0
44	36.11231165856534	39.0	35.0	40.0	30.0	41.0
45	36.16099960406868	39.0	35.0	40.0	29.0	41.0
46	36.01927811531532	39.0	35.0	40.0	28.0	41.0
47	35.667028206560076	38.0	35.0	40.0	27.0	41.0
48	35.69233685888466	38.0	35.0	40.0	27.0	41.0
49	35.801441086837926	39.0	35.0	40.0	28.0	41.0
50	35.652880432609784	38.0	35.0	40.0	27.0	41.0
51	34.39304760074646	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	13.0
10	8.0
11	9.0
12	12.0
13	9.0
14	10.0
15	21.0
16	42.0
17	86.0
18	217.0
19	701.0
20	1537.0
21	2725.0
22	3772.0
23	4705.0
24	5482.0
25	6568.0
26	7590.0
27	8206.0
28	8769.0
29	10305.0
30	13467.0
31	17821.0
32	24395.0
33	32755.0
34	48604.0
35	62263.0
36	60406.0
37	86877.0
38	150644.0
39	217253.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.17122159644152	18.28235448879076	21.57645150099241	8.969972413775315
2	25.759394985987644	39.573142185773044	20.193658134583117	14.4738046936562
3	23.60924286839991	39.23021665310355	23.662506593481705	13.498033885014838
4	22.660942213372163	19.825841805446828	42.25025696845575	15.262959012725258
5	33.299500765424234	25.538215110647972	23.054939017548655	18.107345106379135
6	19.894065801980172	41.81370077135676	25.457352264095217	12.834881162567854
7	66.01864617281436	4.721642225108236	24.348744562392717	4.910967039684699
8	62.99551062888597	17.055999133336	12.87640881263163	7.07208142514641
9	59.57863621649576	5.137563565032687	12.55656852642616	22.72723169204539
10	39.10382815290945	17.53975756622177	24.648852766425026	18.707561514443753
11	32.0202685884597	22.05608296244327	24.809546716671804	21.114101732425226
12	23.23433330710987	18.416481060425312	31.26451694444194	27.084668688022884
13	22.95666551025488	22.454077770197333	38.972796809851076	15.616459909696706
14	21.06780227163984	31.339576237414352	25.852122875415763	21.74049861553005
15	15.912183206579424	23.604342089820953	41.22831566688634	19.25515903671328
16	16.95888633675829	25.717609400209184	26.60348961228393	30.720014650748595
17	18.111472077814046	26.91804221633842	31.734991688021598	23.235494017825935
18	18.02738503482777	25.68317498229916	31.39283996249615	24.896600020376923
19	17.388091365988856	28.66852294402666	27.513228877966746	26.430156812017742
20	20.399748770613897	27.175977931020252	34.820676642760326	17.603596655605525
21	18.891985550441266	34.40191800997437	28.882093715783213	17.824002723801147
22	16.403937646620335	22.933322328076173	32.20211326731039	28.460626757993108
23	17.929756366820698	31.429982705410332	29.66802383841875	20.97223708935022
24	21.830002308524644	24.83572719171201	26.525979930022043	26.808290569741306
25	15.626519402569297	37.14119529989541	24.682771312905686	22.54951398462961
26	16.48402668602904	24.127822622767727	31.475508359051673	27.91264233215156
27	22.551706438204405	27.625817817425364	26.38334147980299	23.43913426456724
28	14.13294264670416	26.701376216005684	34.44666985647167	24.71901128081848
29	19.26766891887535	21.776351679870825	29.426338073761876	29.52964132749195
30	17.082824447662908	30.87155188312417	33.261197311793985	18.78442635741894
31	21.76409973342344	27.765876910497596	23.746722604325324	26.72330075175364
32	21.59347525816141	28.720883894107075	28.253246443388914	21.432394404342606
33	20.120275423756137	28.352809629256097	24.118794872753863	27.4081200742339
34	24.911689259685808	23.93114664032283	24.788395988067894	26.368768111923462
35	18.806479861024236	22.306280605684645	28.769633744181938	30.11760578910918
36	23.58706039693727	28.346619172103736	23.318291382238804	24.748029048720188
37	17.702128098613983	22.763858563530214	38.81042627745887	20.723587060396937
38	17.741205359388278	33.03279523644322	23.541405775438587	25.684593628729914
39	26.425127065581442	22.889731192294942	30.59826899341877	20.086872748704838
40	18.48277053909854	26.424869129866764	28.907629351536716	26.184730979497978
41	26.663846569519478	25.043752345602904	22.58175594896484	25.710645135912774
42	19.532053026424222	20.92735627499558	32.165099492253546	27.375491206326647
43	27.13909312382075	22.095934030361615	25.318969753168417	25.446003092649217
44	21.259319539791097	22.401974755831606	30.56112625050459	25.77757945387271
45	18.645656942920116	21.46257288296038	27.954041014357994	31.937729159761513
46	28.712114079807886	25.122422738580863	25.411439706881854	20.754023474729394
47	16.50491947891827	22.428929038015855	38.89309467401439	22.17305680905148
48	22.036866751699474	24.75950718802353	26.158421536600436	27.045204523676563
49	19.66256849805323	18.750894714510302	37.11875489271809	24.467781894718378
50	23.095950796183068	19.16243114728516	30.810163183029893	26.931454873501874
51	20.293737191879668	19.745881733895462	26.024681868537904	33.935699205686966
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	272.0
1	368.0
2	464.0
3	1969.0
4	3474.0
5	2483.5
6	1493.0
7	1536.5
8	1580.0
9	1561.0
10	1542.0
11	1611.0
12	1680.0
13	1702.0
14	1724.0
15	1701.0
16	1678.0
17	1639.0
18	1600.0
19	1559.0
20	1518.0
21	1653.5
22	1789.0
23	1934.0
24	2079.0
25	2493.0
26	3289.0
27	3671.0
28	4464.5
29	5258.0
30	6498.5
31	7739.0
32	9157.0
33	10575.0
34	12381.5
35	14188.0
36	16450.5
37	18713.0
38	21410.0
39	24107.0
40	27885.0
41	31663.0
42	36563.0
43	41463.0
44	50662.5
45	59862.0
46	124044.5
47	188227.0
48	142414.5
49	96602.0
50	91754.0
51	86906.0
52	69991.0
53	53076.0
54	44283.0
55	35490.0
56	30425.5
57	25361.0
58	21815.5
59	18270.0
60	14809.0
61	11348.0
62	9855.0
63	8362.0
64	6544.0
65	4726.0
66	3786.0
67	2846.0
68	2066.5
69	1287.0
70	1118.0
71	949.0
72	694.5
73	440.0
74	336.0
75	183.0
76	134.0
77	91.0
78	48.0
79	32.0
80	16.0
81	16.0
82	16.0
83	9.0
84	2.0
85	4.0
86	6.0
87	4.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	775387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.07361655198604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.80776031261458	24.41089563140308
2	11.708492769573093	7.744844005251229
3	4.303225821788797	4.269697222993432
4	2.131641001822118	2.8200430848302442
5	1.2999439521950058	2.149692390698544
6	0.882188028762461	1.7506289154025239
7	0.6527995016396525	1.5113308281790214
8	0.5515659935518828	1.459382573908014
9	0.4512567884154631	1.3432224587880348
>10	3.961935889684521	26.34270763591733
>50	0.185617073579697	3.9132733742312777
>100	0.05219253844184909	3.049915780568318
>500	0.0039242510106653455	0.8490807709467609
>1k	0.0047091012127984146	3.4160510381104783
>5k	0.001569700404266138	3.44836854223242
>10k+	0.0011772753031996036	11.520865746539313
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC	31135	4.015414238309386	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTC	29246	3.771793955792398	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG	28385	3.6607526306218703	No Hit
GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	9037	1.165482526789848	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCT	6421	0.8281026119860146	No Hit
GAACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT	5980	0.7712277868986712	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTA	5131	0.6617340760162345	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTC	4859	0.6266548188195056	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATAGCGT	4768	0.6149187438014824	No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	2669	0.34421521124290194	No Hit
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT	2538	0.32732042193124206	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTT	2007	0.2588384896832162	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1764	0.2274993003493739	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATG	1529	0.19719185387425892	No Hit
TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	1483	0.19125933243657683	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGT	1298	0.16740027882850755	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTAT	1251	0.16133878953348457	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC	1098	0.1416067073603246	No Hit
ACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	1056	0.13619005735200615	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTATAGCG	993	0.1280650823395285	No Hit
CGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	832	0.10730125730764122	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.579357146818299E-4	0.0	0.0	0.3466656005323793	0.0
2	2.579357146818299E-4	0.0	0.0	2.2895663713732626	0.0
3	2.579357146818299E-4	0.0	0.0	2.9178977723382	0.0
4	2.579357146818299E-4	0.0	0.0	4.395869417465085	0.0
5	2.579357146818299E-4	0.0	0.0	9.900088600917993	0.0
6	2.579357146818299E-4	0.0	0.0	11.1499161064088	0.0
7	2.579357146818299E-4	0.0	0.0	12.936120930580472	0.0
8	2.579357146818299E-4	0.0	0.0	15.020112537352317	0.0
9	2.579357146818299E-4	0.0	0.0	15.530309380992975	0.0
10	3.869035720227448E-4	0.0	0.0	20.65484719243423	0.0
11	3.869035720227448E-4	0.0	0.0	22.458720613061608	0.0
12	3.869035720227448E-4	0.0	0.0	28.130726978915046	0.0
13	3.869035720227448E-4	0.0	0.0	28.93819473372651	0.0
14	3.869035720227448E-4	0.0	0.0	29.343024837919646	0.0
15	3.869035720227448E-4	0.0	0.0	30.438735753888057	0.0
16	3.869035720227448E-4	0.0	0.0	31.487115466212355	0.0
17	3.869035720227448E-4	0.0	0.0	32.65079244299943	0.0
18	3.869035720227448E-4	0.0	0.0	33.86167165557328	0.0
19	5.158714293636597E-4	0.0	0.0	35.355119443581074	0.0
20	5.158714293636597E-4	0.0	0.0	36.21546401990232	0.0
21	5.158714293636597E-4	0.0	0.0	37.10173113554909	0.0
22	5.158714293636597E-4	0.0	0.0	38.17087467290527	0.0
23	5.158714293636597E-4	0.0	0.0	39.1421316065397	0.0
24	5.158714293636597E-4	0.0	0.0	39.90497648271121	0.0
25	6.448392867045746E-4	0.0	0.0	40.56709746229947	0.0
26	6.448392867045746E-4	0.0	0.0	41.19143085968684	0.0
27	6.448392867045746E-4	0.0	0.0	41.90978182507574	0.0
28	7.738071440454896E-4	0.0	0.0	42.53282554388969	0.0
29	7.738071440454896E-4	0.0	0.0	43.1992024627702	0.0
30	7.738071440454896E-4	0.0	0.0	43.9125236817228	0.0
31	7.738071440454896E-4	0.0	0.0	44.59450571134156	0.0
32	7.738071440454896E-4	0.0	0.0	45.23122002303366	0.0
33	7.738071440454896E-4	0.0	0.0	45.835305466818504	0.0
34	7.738071440454896E-4	0.0	0.0	46.40701997841078	0.0
35	9.027750013864044E-4	0.0	0.0	47.01265303648372	0.0
36	9.027750013864044E-4	0.0	0.0	47.59042903737102	0.0
37	0.0010317428587273195	0.0	0.0	48.15762967395636	0.0
38	0.0011607107160682343	0.0	0.0	48.73669535341707	0.0
39	0.0011607107160682343	0.0	0.0	49.32736814003846	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	20	7.031864E-4	45.000004	20
TAGCGCG	20	7.031864E-4	45.000004	1
ACGATTG	30	2.1645847E-6	45.000004	1
TTCCCGT	20	7.031864E-4	45.000004	29
CCCCGGA	20	7.031864E-4	45.000004	25
AGACCGA	35	1.2112469E-7	45.000004	44
GTCCGTC	20	7.031864E-4	45.000004	9
CGCAGCG	30	2.1645847E-6	45.000004	31
TGCTCGA	20	7.031864E-4	45.000004	16
TTACGCG	20	7.031864E-4	45.000004	1
TTACGAC	20	7.031864E-4	45.000004	19
ACCGTAT	20	7.031864E-4	45.000004	18
CACCGTA	20	7.031864E-4	45.000004	17
CGGTACC	40	6.8102963E-9	45.000004	14
CCCGGTA	40	6.8102963E-9	45.000004	12
CCCGGAG	20	7.031864E-4	45.000004	26
GCGTAAG	20	7.031864E-4	45.000004	1
GCATCGA	20	7.031864E-4	45.000004	9
CTTACCG	20	7.031864E-4	45.000004	1
AACGTAG	45	3.8380676E-10	45.0	1
>>END_MODULE