SRR2936455.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936455.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541833
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC	15943	2.9424195277880822	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG	13393	2.471794815007576	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC	13099	2.417534554004647	No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	7022	1.295971267899888	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT	3237	0.5974165471649013	No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	3102	0.5725011211941686	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCCCACTT	2411	0.4449710519661962	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCCCACTTCGTA	2386	0.44035708419383834	No Hit
GAACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCT	2326	0.4292835615401794	No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT	2254	0.4159953343557886	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTC	2146	0.3960629935792025	No Hit
TCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	1407	0.25967410622830284	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1090	0.2011689948748046	No Hit
ACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	996	0.18382047605073887	No Hit
AGATTAGGGATTAATTTAAGTATTTAATTTTTATCTTCCTTGGATCCGTTT	950	0.17533077534960034	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATG	735	0.13565065250732236	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTT	633	0.11682566399610211	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTAT	631	0.11645654657431348	No Hit
GGAGCTGGGGTCTCTTATTGGAAATATTTAAATGCATTACTTTTCTACTAT	621	0.11461095946537032	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATCCCACTTCGT	616	0.11368816591089874	No Hit
GCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	606	0.11184257880195558	No Hit
CGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	587	0.10833596329496357	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATCCCACT	560	0.10335287810081704	No Hit
AGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	543	0.10021538001561367	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	45	3.8380676E-10	45.000004	10
GGTCGAC	45	3.8380676E-10	45.000004	9
GCTCGGA	75	0.0	45.000004	1
AGCCGTC	20	7.0299825E-4	45.0	20
CGGCGAA	25	3.888066E-5	45.0	31
GTCGCAC	20	7.0299825E-4	45.0	31
ACCGGGT	25	3.888066E-5	45.0	4
TTGTGCG	25	3.888066E-5	45.0	1
CTATCGC	20	7.0299825E-4	45.0	28
TTAGCGA	20	7.0299825E-4	45.0	1
AGCCCGA	25	3.888066E-5	45.0	14
GTTTACG	20	7.0299825E-4	45.0	1
TTTCGCG	25	3.888066E-5	45.0	1
TAGCGTG	20	7.0299825E-4	45.0	13
GATCCGT	135	0.0	45.0	43
GTCTCGC	20	7.0299825E-4	45.0	31
TTCACGG	20	7.0299825E-4	45.0	2
TCCCTCG	20	7.0299825E-4	45.0	31
CGCGCAC	20	7.0299825E-4	45.0	25
CACGACT	25	3.888066E-5	45.0	12

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content