##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936455.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541833
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.826895002703786	33.0	31.0	34.0	30.0	34.0
2	31.843944536416203	33.0	31.0	34.0	30.0	34.0
3	31.92047549706275	33.0	31.0	34.0	30.0	34.0
4	35.63463281121674	37.0	35.0	37.0	33.0	37.0
5	34.62889488089504	37.0	35.0	37.0	33.0	37.0
6	35.1239459390624	37.0	35.0	37.0	32.0	37.0
7	35.67613083736133	37.0	35.0	37.0	33.0	37.0
8	35.58378873195247	37.0	35.0	37.0	33.0	37.0
9	37.52410798161057	39.0	37.0	39.0	35.0	39.0
10	37.22410041470342	39.0	37.0	39.0	34.0	39.0
11	37.1323655074534	39.0	37.0	39.0	34.0	39.0
12	36.976103338113404	39.0	35.0	39.0	33.0	39.0
13	36.725592571881	39.0	35.0	39.0	33.0	39.0
14	37.766121664793395	40.0	36.0	41.0	33.0	41.0
15	37.74799246262225	40.0	36.0	41.0	33.0	41.0
16	37.96656349834728	40.0	36.0	41.0	33.0	41.0
17	37.850769517545075	40.0	36.0	41.0	33.0	41.0
18	37.87933920599151	40.0	36.0	41.0	33.0	41.0
19	37.82144498397108	40.0	36.0	41.0	33.0	41.0
20	37.709977059352234	40.0	36.0	41.0	33.0	41.0
21	37.7938589934537	39.0	36.0	41.0	33.0	41.0
22	37.831206294190274	40.0	36.0	41.0	33.0	41.0
23	37.80664337535735	40.0	36.0	41.0	33.0	41.0
24	37.72035479566582	39.0	36.0	41.0	33.0	41.0
25	37.66089736136411	39.0	36.0	41.0	33.0	41.0
26	37.60613140949333	39.0	35.0	41.0	33.0	41.0
27	37.62784289624294	39.0	36.0	41.0	33.0	41.0
28	37.54600771824529	39.0	36.0	41.0	33.0	41.0
29	37.4899609289209	39.0	36.0	41.0	33.0	41.0
30	37.46190615927786	39.0	36.0	41.0	33.0	41.0
31	37.4625945632695	39.0	36.0	41.0	33.0	41.0
32	37.3235037363911	39.0	35.0	41.0	33.0	41.0
33	37.18029355908555	39.0	35.0	41.0	32.0	41.0
34	37.071784110602344	39.0	35.0	41.0	32.0	41.0
35	37.10343039275939	39.0	35.0	41.0	32.0	41.0
36	37.052100924085465	39.0	35.0	41.0	32.0	41.0
37	37.02638635889656	39.0	35.0	41.0	32.0	41.0
38	37.015205792190585	39.0	35.0	41.0	32.0	41.0
39	36.934435517954796	39.0	35.0	41.0	32.0	41.0
40	36.836521585064034	39.0	35.0	41.0	31.0	41.0
41	36.57454787729799	39.0	35.0	41.0	30.0	41.0
42	36.810321630465474	39.0	35.0	41.0	31.0	41.0
43	36.66236644870283	39.0	35.0	41.0	31.0	41.0
44	36.57233317276725	39.0	35.0	41.0	31.0	41.0
45	36.597612179398446	39.0	35.0	41.0	31.0	41.0
46	36.473721607949315	39.0	35.0	40.0	31.0	41.0
47	36.29964214065958	38.0	35.0	40.0	30.0	41.0
48	36.300697816485894	38.0	35.0	40.0	30.0	41.0
49	36.38914757868199	39.0	35.0	40.0	31.0	41.0
50	36.2195344321959	39.0	35.0	40.0	30.0	41.0
51	35.08394837523739	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	8.0
10	2.0
11	4.0
12	5.0
13	11.0
14	5.0
15	6.0
16	27.0
17	40.0
18	106.0
19	212.0
20	526.0
21	900.0
22	1427.0
23	2044.0
24	2680.0
25	3339.0
26	3886.0
27	4315.0
28	5183.0
29	6371.0
30	8030.0
31	11043.0
32	15202.0
33	21434.0
34	36862.0
35	51498.0
36	40861.0
37	58408.0
38	102873.0
39	164429.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.5305121688786	19.584816723972146	21.903243250226545	10.981427856922705
2	26.551354384099895	34.00771086294117	22.823822100167394	16.617112652791544
3	26.86916448425991	34.50435835395777	24.209304342851027	14.417172818931295
4	25.661412280167507	22.346737832505585	34.7826728899864	17.20917699734051
5	29.670950274346524	26.64160359372722	23.760457557956048	19.92698857397021
6	21.765008775766702	38.0264767926649	25.39786244101042	14.810651990557977
7	68.75125730621797	5.823196446137463	19.669898289694427	5.755647957950143
8	66.39518080294113	13.457652080991744	12.264480015059991	7.882687101007137
9	62.6912720340031	6.119966853255524	13.327907307233042	17.860853805508338
10	40.364466542274094	21.49758320368084	21.471006749312057	16.66694350473301
11	31.930871689247425	21.51382437023954	26.05950541956655	20.49579852094649
12	25.129144957948295	18.875557598005287	32.29131485162402	23.70398259242239
13	21.888109435933213	24.83551204891544	37.33511986165479	15.941258653496556
14	18.13972201766965	31.856125411335228	28.04683361847654	21.95731895251858
15	14.106375949785265	23.515732707310185	42.89568926218964	19.482202080714906
16	14.974724684543023	25.507674873992542	31.096850874716008	28.420749566748427
17	16.06749681174827	25.444186677444897	32.03385544992645	26.45446106088038
18	16.632246467084876	25.246155180655293	33.713155160353836	24.40844319190599
19	17.758792838383783	27.455322950060257	28.60106342729217	26.184820784263785
20	21.150981944621314	26.17559284871907	34.05126671871223	18.622158487947395
21	19.55417997796369	30.903617904409664	30.05261768847597	19.489584429150682
22	16.689090550040326	24.43262038303315	30.037852991604424	28.840436075322103
23	17.069281494482617	29.553017258085056	29.34631150188342	24.031389745548907
24	21.9178233883872	23.190171141292613	27.84105065582938	27.05095481449081
25	15.104284899590834	33.80617275064457	26.369379495158103	24.720162854606492
26	16.089643857055588	27.479869258609202	30.535607834886395	25.894879049448814
27	19.563777030930193	29.36236810973123	27.859875644340597	23.21397921499798
28	13.538488796363454	25.87623861964849	34.15055930517337	26.43471327881469
29	17.49358197082865	24.66571803489267	29.472918777556924	28.367781216721756
30	16.245226850339495	27.3695031494944	32.75529545081233	23.62997454935377
31	20.15879431485347	26.706014583829337	22.905581609093577	30.22960949222362
32	20.327850094032662	28.516720096413472	23.297215193611315	27.85821461594255
33	17.091797657211725	22.976821271498782	26.540465420157133	33.39091565113236
34	18.080663230183468	28.52428700356014	25.65292257946637	27.74212718679003
35	14.70692999503537	23.393923958119935	28.052924055936053	33.84622199090864
36	17.300164441811408	26.93283723951845	29.559661371677254	26.207336946992893
37	15.308591392550841	23.627206168690353	34.60106711846639	26.463135320292412
38	15.237720847567424	23.45390553916059	24.490571818253965	36.81780179501802
39	18.844182617153255	25.330867628955783	28.178793096765975	27.646156657124983
40	17.583277504323288	22.05181301249647	29.28669903826456	31.078210444915683
41	15.361006066444826	28.461537041856072	28.159229873411178	28.018227018287924
42	17.76414504099972	20.93910854451464	31.904479793589534	29.392266620896105
43	21.537078767812222	20.99004674872147	30.21853596956996	27.254338513896347
44	18.686384919338618	22.452305415137136	30.115183091469145	28.746126574055104
45	17.630155416890446	21.749321285340688	26.69512561988657	33.9253976778823
46	24.736219462454297	24.14360144177265	26.943357086039427	24.176822009733627
47	14.890935029797003	21.678819857779057	37.54551679207431	25.884728320349627
48	19.040737644255703	22.431081163384288	28.859814739966005	29.668366452394
49	18.02972502597664	17.568697366162635	37.550684435979356	26.850893171881374
50	20.44449119931787	18.869282601834882	31.44622051443895	29.240005684408292
51	17.777433268184108	19.82880333977443	26.884298298553244	35.50946509348821
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	428.0
1	493.0
2	558.0
3	1248.5
4	1939.0
5	1371.0
6	803.0
7	776.0
8	749.0
9	808.0
10	867.0
11	918.0
12	969.0
13	908.5
14	848.0
15	881.0
16	914.0
17	943.0
18	972.0
19	1124.5
20	1277.0
21	1407.5
22	1538.0
23	1653.0
24	1768.0
25	2569.0
26	3437.5
27	3505.0
28	4133.5
29	4762.0
30	5720.0
31	6678.0
32	7997.5
33	9317.0
34	10180.0
35	11043.0
36	12766.0
37	14489.0
38	15038.5
39	15588.0
40	18402.5
41	21217.0
42	25390.0
43	29563.0
44	35270.5
45	40978.0
46	73597.5
47	106217.0
48	87882.5
49	69548.0
50	67418.0
51	65288.0
52	50960.5
53	36633.0
54	31221.0
55	25809.0
56	21969.5
57	18130.0
58	15503.0
59	12876.0
60	12022.5
61	11169.0
62	9279.5
63	7390.0
64	6583.0
65	5776.0
66	4488.5
67	3201.0
68	2615.0
69	2029.0
70	1866.0
71	1703.0
72	1403.0
73	1103.0
74	736.0
75	291.5
76	214.0
77	151.0
78	88.0
79	100.0
80	112.0
81	67.5
82	23.0
83	16.0
84	9.0
85	5.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	541833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.761439180401975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.7294039007554	18.660592015757498
2	12.55752881451508	6.721150872515813
3	5.288392843961877	4.245750103672764
4	2.7940678855978884	2.9909311114536896
5	1.622928686687225	2.1715953671454913
6	1.0633952569034635	1.7074792495409985
7	0.7540477681365828	1.4125582440273508
8	0.5452171338160202	1.1672636133384409
9	0.399922391896443	0.9632248890855788
>10	4.346756365085003	27.798312937287626
>50	0.7947641310487299	13.672051575565817
>100	0.08562185251958515	4.017832856781201
>500	0.008976484538343604	1.5701387013783688
>1k	0.006214489295776342	3.7620870685373906
>5k	6.904988106418158E-4	1.2975772579826885
>10k+	0.002071496431925447	7.841454135929276
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC	15943	2.9424195277880822	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG	13393	2.471794815007576	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC	13099	2.417534554004647	No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	7022	1.295971267899888	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT	3237	0.5974165471649013	No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	3102	0.5725011211941686	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCCCACTT	2411	0.4449710519661962	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCCCACTTCGTA	2386	0.44035708419383834	No Hit
GAACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCT	2326	0.4292835615401794	No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT	2254	0.4159953343557886	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTC	2146	0.3960629935792025	No Hit
TCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	1407	0.25967410622830284	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1090	0.2011689948748046	No Hit
ACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	996	0.18382047605073887	No Hit
AGATTAGGGATTAATTTAAGTATTTAATTTTTATCTTCCTTGGATCCGTTT	950	0.17533077534960034	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATG	735	0.13565065250732236	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTT	633	0.11682566399610211	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTAT	631	0.11645654657431348	No Hit
GGAGCTGGGGTCTCTTATTGGAAATATTTAAATGCATTACTTTTCTACTAT	621	0.11461095946537032	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATCCCACTTCGT	616	0.11368816591089874	No Hit
GCCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	606	0.11184257880195558	No Hit
CGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	587	0.10833596329496357	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATCCCACT	560	0.10335287810081704	No Hit
AGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	543	0.10021538001561367	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43832693837400083	0.0
2	0.0	0.0	0.0	2.870810747961088	0.0
3	0.0	0.0	0.0	3.6418970420775403	0.0
4	0.0	0.0	0.0	4.9670285862987305	0.0
5	0.0	0.0	0.0	9.700959520737939	0.0
6	0.0	0.0	0.0	11.010034457111324	0.0
7	0.0	0.0	0.0	12.874815671987495	0.0
8	0.0	0.0	0.0	15.181614999455551	0.0
9	0.0	0.0	0.0	15.85285503097818	0.0
10	0.0	0.0	0.0	19.98346353950387	0.0
11	0.0	0.0	0.0	22.106073273499398	0.0
12	0.0	0.0	0.0	27.019395274927884	0.0
13	0.0	0.0	0.0	27.896418269097673	0.0
14	0.0	0.0	0.0	28.28233053357769	0.0
15	0.0	0.0	0.0	29.261968171004717	0.0
16	0.0	0.0	0.0	30.386299837772892	0.0
17	0.0	0.0	0.0	31.690576247663024	0.0
18	0.0	0.0	0.0	33.065723202536574	0.0
19	0.0	0.0	0.0	34.38771724867256	0.0
20	0.0	0.0	0.0	35.25071378081439	0.0
21	1.8455871089431616E-4	0.0	0.0	36.12220001365734	0.0
22	1.8455871089431616E-4	0.0	0.0	37.09482442007039	0.0
23	1.8455871089431616E-4	0.0	0.0	37.9939944595475	0.0
24	1.8455871089431616E-4	0.0	0.0	38.71100505137192	0.0
25	1.8455871089431616E-4	0.0	0.0	39.307498804982345	0.0
26	3.691174217886323E-4	0.0	0.0	39.89476462304806	0.0
27	3.691174217886323E-4	0.0	0.0	40.51894218329264	0.0
28	3.691174217886323E-4	0.0	0.0	41.05120950551185	0.0
29	3.691174217886323E-4	0.0	0.0	41.6176201892465	0.0
30	3.691174217886323E-4	0.0	0.0	42.30565506346051	0.0
31	3.691174217886323E-4	0.0	0.0	42.8903370595737	0.0
32	5.536761326829484E-4	0.0	0.0	43.47243523373438	0.0
33	5.536761326829484E-4	0.0	0.0	44.00544079079717	0.0
34	5.536761326829484E-4	0.0	0.0	44.53733899559458	0.0
35	5.536761326829484E-4	0.0	0.0	45.09655188960436	0.0
36	5.536761326829484E-4	0.0	0.0	45.64284567385154	0.0
37	5.536761326829484E-4	0.0	0.0	46.14466080877318	0.0
38	5.536761326829484E-4	0.0	0.0	46.673790632907185	0.0
39	5.536761326829484E-4	0.0	0.0	47.191662375676636	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	45	3.8380676E-10	45.000004	10
GGTCGAC	45	3.8380676E-10	45.000004	9
GCTCGGA	75	0.0	45.000004	1
AGCCGTC	20	7.0299825E-4	45.0	20
CGGCGAA	25	3.888066E-5	45.0	31
GTCGCAC	20	7.0299825E-4	45.0	31
ACCGGGT	25	3.888066E-5	45.0	4
TTGTGCG	25	3.888066E-5	45.0	1
CTATCGC	20	7.0299825E-4	45.0	28
TTAGCGA	20	7.0299825E-4	45.0	1
AGCCCGA	25	3.888066E-5	45.0	14
GTTTACG	20	7.0299825E-4	45.0	1
TTTCGCG	25	3.888066E-5	45.0	1
TAGCGTG	20	7.0299825E-4	45.0	13
GATCCGT	135	0.0	45.0	43
GTCTCGC	20	7.0299825E-4	45.0	31
TTCACGG	20	7.0299825E-4	45.0	2
TCCCTCG	20	7.0299825E-4	45.0	31
CGCGCAC	20	7.0299825E-4	45.0	25
CACGACT	25	3.888066E-5	45.0	12
>>END_MODULE