##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936454.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	878355
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.994457821723564	33.0	31.0	34.0	30.0	34.0
2	32.03660934360253	33.0	31.0	34.0	30.0	34.0
3	32.067818820408604	33.0	31.0	34.0	30.0	34.0
4	35.737642524947205	37.0	35.0	37.0	33.0	37.0
5	34.745026783020535	37.0	35.0	37.0	33.0	37.0
6	35.26737139311554	37.0	35.0	37.0	33.0	37.0
7	35.87295569559005	37.0	35.0	37.0	35.0	37.0
8	35.87599660729432	37.0	35.0	37.0	35.0	37.0
9	37.713410864627626	39.0	38.0	39.0	35.0	39.0
10	37.36689151880504	39.0	37.0	39.0	34.0	39.0
11	37.270349687768615	39.0	37.0	39.0	34.0	39.0
12	37.07505735152643	39.0	37.0	39.0	34.0	39.0
13	36.928153195461974	39.0	36.0	39.0	33.0	39.0
14	37.77512850726642	40.0	37.0	41.0	33.0	41.0
15	37.9073347336783	40.0	36.0	41.0	33.0	41.0
16	38.00096999504756	40.0	36.0	41.0	33.0	41.0
17	38.049234079614735	40.0	36.0	41.0	33.0	41.0
18	38.02686840742069	40.0	36.0	41.0	33.0	41.0
19	37.97416648166174	40.0	36.0	41.0	34.0	41.0
20	37.9628202719857	40.0	36.0	41.0	33.0	41.0
21	37.82871845666046	40.0	36.0	41.0	33.0	41.0
22	37.90113564561026	40.0	36.0	41.0	34.0	41.0
23	37.945071184202284	40.0	36.0	41.0	34.0	41.0
24	37.90286046074765	40.0	36.0	41.0	34.0	41.0
25	37.749438438899986	40.0	35.0	41.0	33.0	41.0
26	37.80961228660394	40.0	35.0	41.0	34.0	41.0
27	37.774579754199614	40.0	35.0	41.0	34.0	41.0
28	37.704657000870945	40.0	35.0	41.0	33.0	41.0
29	37.67481257578086	40.0	35.0	41.0	33.0	41.0
30	37.53960983884648	40.0	35.0	41.0	33.0	41.0
31	37.49379123475133	40.0	35.0	41.0	33.0	41.0
32	37.449741846975314	40.0	35.0	41.0	33.0	41.0
33	37.316237739866	39.0	35.0	41.0	33.0	41.0
34	37.169368877048576	39.0	35.0	41.0	32.0	41.0
35	37.124623870758406	39.0	35.0	41.0	32.0	41.0
36	37.067766449783974	39.0	35.0	41.0	32.0	41.0
37	37.0049068998298	39.0	35.0	41.0	32.0	41.0
38	36.91039272276016	39.0	35.0	41.0	32.0	41.0
39	36.872468421082594	39.0	35.0	41.0	31.0	41.0
40	36.72541170711159	39.0	35.0	41.0	31.0	41.0
41	36.60821080314907	39.0	35.0	41.0	31.0	41.0
42	36.62170534692692	39.0	35.0	41.0	31.0	41.0
43	36.50959463998042	39.0	35.0	41.0	31.0	41.0
44	36.382841789481475	38.0	35.0	41.0	31.0	41.0
45	36.3496957380558	38.0	35.0	40.0	31.0	41.0
46	36.25120253200585	38.0	35.0	40.0	31.0	41.0
47	36.14561879877726	38.0	35.0	40.0	30.0	41.0
48	36.100541352869854	38.0	35.0	40.0	30.0	41.0
49	36.08528670070757	38.0	35.0	40.0	30.0	41.0
50	35.95335598932095	38.0	35.0	40.0	30.0	41.0
51	34.736703269179316	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	13.0
10	25.0
11	18.0
12	19.0
13	24.0
14	29.0
15	37.0
16	65.0
17	121.0
18	217.0
19	367.0
20	658.0
21	967.0
22	1521.0
23	2257.0
24	3180.0
25	4707.0
26	6114.0
27	7505.0
28	9251.0
29	10824.0
30	13547.0
31	17774.0
32	23769.0
33	34761.0
34	64698.0
35	77004.0
36	67133.0
37	100501.0
38	166374.0
39	264695.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.13831537362456	21.281827962498078	25.77306442156076	10.806792242316602
2	32.13575376698488	23.710914152022816	25.54001514194147	18.61331693905084
3	31.761076102487035	23.47581558709178	27.356365023253698	17.40674328716749
4	29.5965754165457	25.30810435416204	25.721946137951058	19.3733740913412
5	24.343687916616858	30.876923339651963	25.503811101433932	19.275577642297247
6	25.760541011322303	33.53461869062053	25.0454542867064	15.659386011350765
7	79.16263925178316	6.036397584120316	9.11339947970923	5.6875636843872925
8	79.45227157584348	5.061051624912478	9.201177200562414	6.285499598681627
9	73.47370937718803	7.2135981465352845	11.928775950498375	7.383916525778302
10	43.10330105708967	23.167739695225734	18.11192513277661	15.617034114907982
11	31.9253604749788	23.125387798782953	23.529324703565187	21.419927022673065
12	29.437300408149326	20.54203596495722	29.55934673338229	20.461316893511167
13	27.257999328289817	22.821068929988446	30.750778443795507	19.170153297926237
14	21.556204495904275	26.687614916520086	30.515452180496496	21.240728407079143
15	19.03660820511069	24.52561891262644	35.75205924711535	20.685713635147522
16	24.296326656078694	24.47393138309681	30.101041150787548	21.128700810036943
17	25.213267983901726	24.036067421486756	26.31111566507847	24.439548929533046
18	25.100101895019666	23.674482413147306	30.82762664298604	20.39778904884699
19	25.757694781722652	25.689612969699038	27.153371928206703	21.399320320371604
20	26.644124528237445	26.250547899197933	27.62482139909262	19.480506173472
21	24.983178783066073	26.039129964535977	29.62355767315038	19.35413357924757
22	23.271228603468984	23.309026532552327	29.216205292848564	24.203539571130122
23	22.565022115203988	24.60542719060061	29.241024414957508	23.588526279237893
24	23.83239123133585	23.901042289279392	28.41425164085136	23.852314838533395
25	22.722475536656592	25.044998889970454	27.150639547791037	25.081886025581912
26	21.116518947350446	25.235582423962978	26.62021619960039	27.02768242908619
27	20.72613009546254	26.320678996533292	29.383222045755986	23.56996886224818
28	18.355220838954637	25.16818370704328	29.772358556620045	26.70423689738204
29	20.283826015677032	25.620506515019553	29.076626193281758	25.019041276021653
30	22.29212562118961	25.070728805551283	28.542787369571528	24.094358203687573
31	22.51265149057044	24.621366076358647	28.727223047628808	24.138759385442103
32	23.567008783464544	24.74625863119126	27.304563644540075	24.382168940804117
33	24.24885154635654	24.503532170933166	26.730536058882798	24.517080223827495
34	19.983150320770076	22.808887067301946	31.945739478912284	25.262223133015695
35	19.874879746799415	25.580431602256493	28.358010143962293	26.186678506981803
36	24.59153759015432	25.029515400948366	26.595738625043403	23.78320838385391
37	21.737566245993932	25.895566143529663	30.467180126486443	21.899687483989958
38	20.755730883298895	26.691258090407636	27.785348748512845	24.767662277780627
39	22.20958496279978	23.00641540151761	29.1810258949969	25.602973740685712
40	22.791354292968105	21.60846127135384	29.99447831457668	25.605706121101377
41	20.45983685411935	22.907822008185757	27.64838818017772	28.98395295751718
42	22.48828776519744	23.08724832214765	28.648439412310513	25.77602450034439
43	22.601681552447474	22.47929367966255	29.47509833723267	25.44392643065731
44	20.969539650824554	22.744220730797913	29.77509093703571	26.51114868134183
45	21.195074884300766	22.72190629073666	27.701555749099168	28.381463075863405
46	22.215049723631104	23.109562762208903	28.678040200146864	25.997347314013126
47	18.952815205697014	24.08741340346443	31.731930711386624	25.22784067945193
48	20.592015756727065	23.31403589664771	29.940741499735303	26.153206846889926
49	22.699478001491425	21.20543516004349	31.51504801589335	24.58003882257174
50	20.55706405724337	22.470071895759688	30.48710373368399	26.485760313312955
51	20.189558891336645	22.177137945363775	27.55343796073342	30.079865202566157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	441.0
1	791.5
2	1142.0
3	2016.5
4	2891.0
5	2047.0
6	1203.0
7	1198.5
8	1194.0
9	1271.5
10	1349.0
11	1432.5
12	1516.0
13	1579.0
14	1642.0
15	1596.5
16	1551.0
17	1568.0
18	1585.0
19	1534.5
20	1484.0
21	1686.5
22	1889.0
23	2050.5
24	2212.0
25	3002.5
26	4489.5
27	5186.0
28	7191.5
29	9197.0
30	9577.0
31	9957.0
32	11827.5
33	13698.0
34	16100.0
35	18502.0
36	19807.0
37	21112.0
38	25015.5
39	28919.0
40	32765.0
41	36611.0
42	40464.5
43	44318.0
44	50303.5
45	56289.0
46	67066.0
47	77843.0
48	88037.5
49	98232.0
50	96482.5
51	94733.0
52	80983.5
53	67234.0
54	59929.5
55	52625.0
56	49598.0
57	46571.0
58	44799.5
59	43028.0
60	39947.5
61	36867.0
62	33784.0
63	30701.0
64	27285.0
65	23869.0
66	19825.5
67	15782.0
68	12776.0
69	9770.0
70	7911.0
71	6052.0
72	4761.0
73	3470.0
74	2921.0
75	1579.5
76	787.0
77	616.0
78	445.0
79	301.5
80	158.0
81	106.5
82	55.0
83	49.5
84	44.0
85	27.5
86	11.0
87	8.5
88	6.0
89	6.0
90	6.0
91	5.5
92	5.0
93	5.0
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	878355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.9273234341539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.2756068261713	20.32885208192091
2	12.519652342995741	7.243200652180009
3	4.85165544909375	4.2103621910103035
4	2.4576668427779507	2.8437489461773455
5	1.3970473146123599	2.0206419761303946
6	0.9283727114768929	1.6113202615400717
7	0.6817315369235029	1.38044680646943
8	0.5001929663486389	1.1575394973644748
9	0.39800463379629886	1.0361887893105766
>10	5.0652369027163235	36.82225802390383
>50	0.8478952957285318	15.228289773554266
>100	0.06979867636661927	3.596387358177497
>500	0.004362417272913702	0.8515736820871974
>1k	0.0027760837191269016	1.669189960173612
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	4209	0.47919121539696363	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2915	0.33187037131911357	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	2304	0.2623085199036836	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1489	0.16952143495511496	No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	1476	0.1680413955632973	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1144	0.130243466479954	No Hit
ACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	1013	0.11532922337779143	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	936	0.10656283621087147	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4154755195792136E-4	0.0	0.0	0.2804105401574534	0.0
2	3.4154755195792136E-4	0.0	0.0	1.441216820078442	0.0
3	3.4154755195792136E-4	0.0	0.0	1.983594332587621	0.0
4	3.4154755195792136E-4	0.0	0.0	2.6524582885052173	0.0
5	3.4154755195792136E-4	0.0	0.0	4.384673622851809	0.0
6	3.4154755195792136E-4	0.0	0.0	5.278275867957716	0.0
7	3.4154755195792136E-4	0.0	0.0	6.207626756835221	0.0
8	3.4154755195792136E-4	0.0	0.0	7.699164916235463	0.0
9	3.4154755195792136E-4	0.0	0.0	8.227425129930381	0.0
10	3.4154755195792136E-4	0.0	0.0	9.449254572467852	0.0
11	3.4154755195792136E-4	0.0	0.0	11.22792037388072	0.0
12	3.4154755195792136E-4	0.0	0.0	12.681205207461675	0.0
13	4.5539673594389513E-4	0.0	0.0	13.22938902835414	0.0
14	5.69245919929869E-4	0.0	0.0	13.455151960198325	0.0
15	5.69245919929869E-4	0.0	0.0	13.90349004673509	0.0
16	5.69245919929869E-4	0.0	0.0	14.775347097699678	0.0
17	5.69245919929869E-4	0.0	0.0	15.828793597121892	0.0
18	5.69245919929869E-4	0.0	0.0	17.078516089735928	0.0
19	5.69245919929869E-4	0.0	0.0	17.789162696176376	0.0
20	5.69245919929869E-4	0.0	0.0	18.51996060818234	0.0
21	5.69245919929869E-4	0.0	0.0	19.367340084589944	0.0
22	5.69245919929869E-4	0.0	0.0	20.24090487331432	0.0
23	5.69245919929869E-4	0.0	0.0	21.063237529245008	0.0
24	5.69245919929869E-4	0.0	0.0	21.77012711261392	0.0
25	5.69245919929869E-4	0.0	0.0	22.38514040450615	0.0
26	5.69245919929869E-4	0.0	0.0	22.950287753812525	0.0
27	5.69245919929869E-4	0.0	0.0	23.571562750823983	0.0
28	5.69245919929869E-4	0.0	0.0	24.127488316227492	0.0
29	5.69245919929869E-4	0.0	0.0	24.744209345879515	0.0
30	5.69245919929869E-4	0.0	0.0	25.475918051357368	0.0
31	5.69245919929869E-4	0.0	0.0	26.038788416984023	0.0
32	5.69245919929869E-4	0.0	0.0	26.623973222671925	0.0
33	5.69245919929869E-4	0.0	0.0	27.204490211816406	0.0
34	5.69245919929869E-4	0.0	0.0	27.818251162684792	0.0
35	6.830951039158427E-4	0.0	0.0	28.47015159018848	0.0
36	6.830951039158427E-4	0.0	0.0	29.01719691924108	0.0
37	7.969442879018165E-4	0.0	0.0	29.59179375081829	0.0
38	7.969442879018165E-4	0.0	0.0	30.21648422334933	0.0
39	7.969442879018165E-4	0.0	0.0	31.166214116160322	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.0323766E-4	45.000004	1
GCCCAAT	20	7.0323766E-4	45.000004	45
ATTGTCG	40	6.8121153E-9	45.000004	1
AAGCGTA	20	7.0323766E-4	45.000004	32
AGGCGAA	20	7.0323766E-4	45.000004	11
ATCCCGC	20	7.0323766E-4	45.000004	23
ACTACGC	20	7.0323766E-4	45.000004	45
TTACGTT	20	7.0323766E-4	45.000004	24
CCGAATC	20	7.0323766E-4	45.000004	25
CGCATCG	25	3.8900525E-5	45.0	21
GCGTTTA	25	3.8900525E-5	45.0	32
CGAATCG	25	3.8900525E-5	45.0	36
GCGATAT	70	0.0	41.785713	9
CGGTCTA	70	0.0	41.785713	31
GTCGGTA	70	0.0	41.785713	10
CGTATGG	135	0.0	41.666664	2
ATAACGC	60	3.6379788E-12	41.249996	11
GCTACGA	55	6.002665E-11	40.909092	10
AACGGGC	155	0.0	40.645164	4
TTACGGG	155	0.0	40.645164	3
>>END_MODULE