##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936453.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391123
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.862531735540994	33.0	31.0	34.0	30.0	34.0
2	31.91264129186982	33.0	31.0	34.0	30.0	34.0
3	31.937232021640252	33.0	31.0	34.0	30.0	34.0
4	35.631279674168994	37.0	35.0	37.0	33.0	37.0
5	34.712778333158624	37.0	35.0	37.0	33.0	37.0
6	35.19305435885898	37.0	35.0	37.0	33.0	37.0
7	35.778596502890395	37.0	35.0	37.0	35.0	37.0
8	35.764746639803846	37.0	35.0	37.0	35.0	37.0
9	37.59077323501814	39.0	37.0	39.0	35.0	39.0
10	37.29175221094132	39.0	37.0	39.0	34.0	39.0
11	37.23518688494412	39.0	37.0	39.0	34.0	39.0
12	37.07311766375283	39.0	37.0	39.0	34.0	39.0
13	36.91693661584719	39.0	37.0	39.0	33.0	39.0
14	37.908356194854306	40.0	37.0	41.0	33.0	41.0
15	38.03292314693843	40.0	37.0	41.0	33.0	41.0
16	38.05586477911041	40.0	36.0	41.0	34.0	41.0
17	38.07907998251189	40.0	36.0	41.0	34.0	41.0
18	38.07006235889988	40.0	36.0	41.0	34.0	41.0
19	37.97971226442833	40.0	36.0	41.0	34.0	41.0
20	37.993053336162795	40.0	36.0	41.0	34.0	41.0
21	37.86983122956205	40.0	36.0	41.0	34.0	41.0
22	37.90243989742357	40.0	36.0	41.0	34.0	41.0
23	37.92788969198948	40.0	36.0	41.0	34.0	41.0
24	37.900714097611235	40.0	36.0	41.0	34.0	41.0
25	37.7366250514544	39.0	35.0	41.0	33.0	41.0
26	37.81007253472693	40.0	36.0	41.0	34.0	41.0
27	37.82031483702058	40.0	36.0	41.0	34.0	41.0
28	37.75257144172038	40.0	36.0	41.0	33.0	41.0
29	37.73498873755826	40.0	36.0	41.0	33.0	41.0
30	37.57439731235443	39.0	36.0	41.0	33.0	41.0
31	37.49078422900213	39.0	35.0	41.0	33.0	41.0
32	37.43927102215927	39.0	35.0	41.0	33.0	41.0
33	37.29798298744896	39.0	35.0	41.0	33.0	41.0
34	37.197226959294134	39.0	35.0	41.0	32.0	41.0
35	37.14387545605858	39.0	35.0	41.0	32.0	41.0
36	37.0707884731913	39.0	35.0	41.0	32.0	41.0
37	37.01962553979183	39.0	35.0	41.0	32.0	41.0
38	36.92341539617972	39.0	35.0	41.0	32.0	41.0
39	36.876926184346104	39.0	35.0	41.0	32.0	41.0
40	36.74084878669881	39.0	35.0	41.0	31.0	41.0
41	36.679379632494125	39.0	35.0	41.0	31.0	41.0
42	36.736732434553836	39.0	35.0	41.0	31.0	41.0
43	36.60382795182078	39.0	35.0	41.0	31.0	41.0
44	36.443806679740135	39.0	35.0	41.0	31.0	41.0
45	36.381000350273446	39.0	35.0	41.0	31.0	41.0
46	36.231134451310716	38.0	35.0	40.0	30.0	41.0
47	36.135739907906206	38.0	35.0	40.0	30.0	41.0
48	36.1240274798465	38.0	35.0	40.0	30.0	41.0
49	36.126264627751375	38.0	35.0	40.0	30.0	41.0
50	35.95830467653398	38.0	35.0	40.0	30.0	41.0
51	34.71149484944634	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	5.0
11	10.0
12	8.0
13	4.0
14	9.0
15	15.0
16	25.0
17	47.0
18	72.0
19	137.0
20	304.0
21	430.0
22	682.0
23	1090.0
24	1605.0
25	2294.0
26	3070.0
27	3476.0
28	4075.0
29	4785.0
30	5911.0
31	7620.0
32	10370.0
33	14435.0
34	26707.0
35	37295.0
36	29154.0
37	43186.0
38	76456.0
39	117789.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16786790856074	21.71797618651933	25.12099774239817	11.993158162521764
2	32.44043433906981	24.12335761384526	24.31562449664172	19.12058355044321
3	31.375040588254844	23.640900688530206	27.98173464613434	17.002324077080612
4	28.717053203212288	25.94196710497721	26.56274368932535	18.77823600248515
5	23.975578015100112	30.07877317365637	26.159290044308314	19.786358766935212
6	25.561524124124634	32.243053975347905	26.69467149720165	15.500750403325808
7	74.59622676242512	6.980924159407655	12.501693840556552	5.921155237610675
8	72.47285380813709	6.672836933650029	12.257013778274354	8.597295479938536
9	66.06310546810083	8.621073166241821	16.03306376766388	9.28275759799347
10	38.10438148613096	25.189262712752765	21.67757968720837	15.028776113907902
11	30.486061929367487	23.4299696003559	26.03835622042171	20.045612249854905
12	27.877930983347948	20.36699452601867	32.183226248520285	19.571848242113095
13	23.404913543821255	27.037786067298526	32.16200530267972	17.395295086200505
14	18.698465700048324	29.966787941389285	29.77707779905554	21.55766855950686
15	16.428847191292764	25.237329433451883	39.28431720967573	19.049506165579626
16	17.539239574251578	25.00926818417736	34.768346530375354	22.68314571119571
17	17.90536480851292	24.81904669375107	29.937641100114288	27.33794739762172
18	21.01129312262383	24.24224604536169	32.88556285362916	21.860897978385317
19	21.469461013543054	26.859069908954474	28.843100508024328	22.828368569478144
20	24.010605359439356	25.846345011671517	29.488166126768306	20.654883502120818
21	22.393978364862203	27.53583910943616	29.7947193082483	20.275463217453332
22	20.232509977679655	25.155002390552333	28.429675575202683	26.182812056565325
23	18.97996282499367	26.91582954722683	29.525750211570273	24.578457416209222
24	21.520851496843704	22.958762333076805	30.820483581891118	24.699902588188372
25	17.96135742464647	27.114743955226363	29.005964875499522	25.917933744627646
26	19.809880779192223	26.875688721962142	29.11641606348898	24.19801443535665
27	20.875530204053454	27.262779228017784	28.263487445125957	23.5982031228028
28	17.24853818364044	25.952961088966898	32.99652538971117	23.801975337681498
29	22.816096215257094	22.820442674043715	29.546715483364565	24.816745627334626
30	21.47125073186696	23.98248121434945	30.66912454649816	23.87714350728543
31	22.60056299424989	24.97603055816201	27.22110435847547	25.20230208911263
32	24.601723754419965	25.277470258716566	27.742423738823845	22.378382248039618
33	21.24139976426853	24.35065184098097	30.33700396039098	24.07094443435952
34	19.519690736673628	22.615136414887388	32.755936112169316	25.109236736269665
35	20.36699452601867	22.42747166492382	31.40418742952984	25.80134637952767
36	22.563234583494197	22.913763700932954	32.514579812488655	22.008421903084198
37	20.28466748311912	23.842883185084997	33.00956476607103	22.862884565724848
38	19.446312285393596	25.138894925637207	31.785141758474957	23.629651030494244
39	22.05367620927432	21.851182364626982	32.90627245137719	23.188868974721508
40	22.493946916954513	20.25015148687242	33.137401789206976	24.118499806966096
41	17.94959641851796	22.5913587285841	30.442597341501266	29.01644751139667
42	21.480199323486474	20.395885693247394	32.84721174668838	25.276703236577752
43	23.454514308798	20.658974286861167	32.37626015345556	23.510251250885272
44	21.080069441070968	21.878283813531805	31.231607448296316	25.81003929710091
45	21.00438992337449	21.782150372133575	29.51833566422839	27.695124040263547
46	23.204976439636635	21.882374598272154	30.817415493335854	24.095233468755353
47	18.300125535956717	21.509857512854012	34.90231973062182	25.287697220567445
48	19.55804184361441	19.651874218596195	34.122258215446294	26.667825722343096
49	21.001321834819226	17.942181871176075	36.48775449155381	24.56874180245089
50	20.334012574049595	19.490543895398634	33.977291031210136	26.19815249934164
51	19.0824881175487	19.144361236746494	29.874745284731404	31.898405360973403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	357.0
1	511.5
2	666.0
3	1264.0
4	1862.0
5	1298.5
6	735.0
7	784.0
8	833.0
9	857.0
10	881.0
11	1009.0
12	1137.0
13	1118.5
14	1100.0
15	1055.0
16	1010.0
17	1022.5
18	1035.0
19	1260.5
20	1486.0
21	1441.5
22	1397.0
23	1580.5
24	1764.0
25	2014.5
26	2441.5
27	2618.0
28	3358.5
29	4099.0
30	4527.0
31	4955.0
32	5406.0
33	5857.0
34	7380.0
35	8903.0
36	9549.0
37	10195.0
38	10728.5
39	11262.0
40	13316.5
41	15371.0
42	19387.0
43	23403.0
44	25795.5
45	28188.0
46	35594.5
47	43001.0
48	47496.5
49	51992.0
50	50970.0
51	49948.0
52	40489.5
53	31031.0
54	26621.0
55	22211.0
56	19473.5
57	16736.0
58	14757.0
59	12778.0
60	11747.5
61	10717.0
62	9287.5
63	7858.0
64	6688.5
65	5519.0
66	4627.5
67	3736.0
68	2572.5
69	1409.0
70	1274.0
71	1139.0
72	910.5
73	682.0
74	565.5
75	331.5
76	214.0
77	167.0
78	120.0
79	123.5
80	127.0
81	85.5
82	44.0
83	27.5
84	11.0
85	14.5
86	18.0
87	10.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	391123.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.02808597421872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.963466261856	22.40795912489075
2	12.701687677517631	8.136214899064182
3	5.216967695199124	5.012684695996777
4	2.649234500955819	3.393996414499176
5	1.587045169109227	2.5415009560599406
6	1.0472908639502603	2.012563309836764
7	0.7416792540643078	1.6628196840126241
8	0.5090645155111313	1.3043489655371612
9	0.43233580719023246	1.246219956217981
>10	4.587038607572403	35.045911344555805
>50	0.48107955741994735	9.589084243285741
>100	0.0735204639246431	3.949786415117725
>500	0.0063930838195341835	1.3762313085852778
>1k	0.0031965419097670917	2.320678682340099
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	3336	0.8529286183630214	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2582	0.6601503874740172	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	1874	0.4791331627135198	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	1275	0.3259844089966583	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	910	0.23266338210741888	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	812	0.20760732557277378	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	730	0.1866420537784789	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	633	0.16184167129010565	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	631	0.1613303231975619	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	618	0.15800656059602733	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC	534	0.13652994070918867	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	509	0.13013808955239145	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	490	0.12528028267322555	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	466	0.11914410556270022	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	429	0.10968416585064034	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.556740462718889E-4	0.0	0.0	0.5044448932944368	0.0
2	2.556740462718889E-4	0.0	0.0	2.678952656836852	0.0
3	2.556740462718889E-4	0.0	0.0	3.5822490623154355	0.0
4	2.556740462718889E-4	0.0	0.0	4.679090720821839	0.0
5	2.556740462718889E-4	0.0	0.0	7.398695551015921	0.0
6	2.556740462718889E-4	0.0	0.0	8.78342618562447	0.0
7	2.556740462718889E-4	0.0	0.0	10.24511470816086	0.0
8	2.556740462718889E-4	0.0	0.0	12.619048227795348	0.0
9	2.556740462718889E-4	0.0	0.0	13.562996806631162	0.0
10	2.556740462718889E-4	0.0	0.0	15.551629538533914	0.0
11	2.556740462718889E-4	0.0	0.0	18.014026278178477	0.0
12	2.556740462718889E-4	0.0	0.0	20.284923157165395	0.0
13	2.556740462718889E-4	0.0	0.0	21.12506807321482	0.0
14	2.556740462718889E-4	0.0	0.0	21.465625902848977	0.0
15	2.556740462718889E-4	0.0	0.0	22.112225565870585	0.0
16	2.556740462718889E-4	0.0	0.0	23.40056708503463	0.0
17	2.556740462718889E-4	0.0	0.0	24.861999933524746	0.0
18	2.556740462718889E-4	0.0	0.0	26.598026707710872	0.0
19	2.556740462718889E-4	0.0	0.0	27.513339793364235	0.0
20	2.556740462718889E-4	0.0	0.0	28.40973299959348	0.0
21	2.556740462718889E-4	0.0	0.0	29.449303671734977	0.0
22	2.556740462718889E-4	0.0	0.0	30.473278227053893	0.0
23	2.556740462718889E-4	0.0	0.0	31.472452399884435	0.0
24	2.556740462718889E-4	0.0	0.0	32.25762739598541	0.0
25	2.556740462718889E-4	0.0	0.0	32.96584450415854	0.0
26	2.556740462718889E-4	0.0	0.0	33.61576792978168	0.0
27	2.556740462718889E-4	0.0	0.0	34.20867604308619	0.0
28	2.556740462718889E-4	0.0	0.0	34.79928308997425	0.0
29	2.556740462718889E-4	0.0	0.0	35.41545754148951	0.0
30	2.556740462718889E-4	0.0	0.0	36.19526338261877	0.0
31	2.556740462718889E-4	0.0	0.0	36.89836700986646	0.0
32	2.556740462718889E-4	0.0	0.0	37.54445532479552	0.0
33	5.113480925437778E-4	0.0	0.0	38.09594424260399	0.0
34	5.113480925437778E-4	0.0	0.0	38.691153422324945	0.0
35	5.113480925437778E-4	0.0	0.0	39.34337791436454	0.0
36	5.113480925437778E-4	0.0	0.0	39.97898359339645	0.0
37	5.113480925437778E-4	0.0	0.0	40.57828355785776	0.0
38	5.113480925437778E-4	0.0	0.0	41.186787787984855	0.0
39	5.113480925437778E-4	0.0	0.0	41.79708173643585	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	20	7.027572E-4	45.000004	39
TATCACC	20	7.027572E-4	45.000004	14
CCGGGAT	20	7.027572E-4	45.000004	5
GCGGTAA	20	7.027572E-4	45.000004	25
GGGTCGA	20	7.027572E-4	45.000004	17
ATGTGCG	20	7.027572E-4	45.000004	12
GAGTACG	20	7.027572E-4	45.000004	1
GCTATCG	20	7.027572E-4	45.000004	28
CGATTAT	20	7.027572E-4	45.000004	10
GCGTTAG	20	7.027572E-4	45.000004	1
TAGCCCA	20	7.027572E-4	45.000004	14
ATCCGTC	20	7.027572E-4	45.000004	28
AACGGTC	20	7.027572E-4	45.000004	2
CGTTAAC	20	7.027572E-4	45.000004	41
CCCGTTG	20	7.027572E-4	45.000004	12
AAACGCC	20	7.027572E-4	45.000004	12
ACAGCCC	20	7.027572E-4	45.000004	28
CGGGATC	40	6.7957444E-9	45.000004	6
AACGAGT	20	7.027572E-4	45.000004	30
CTCGCGA	20	7.027572E-4	45.000004	15
>>END_MODULE