##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936452.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	853603
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.981669464610597	33.0	31.0	34.0	30.0	34.0
2	32.03332345364297	33.0	31.0	34.0	30.0	34.0
3	32.05946441144185	33.0	31.0	34.0	30.0	34.0
4	35.722626326289856	37.0	35.0	37.0	33.0	37.0
5	34.80068954771715	37.0	35.0	37.0	33.0	37.0
6	35.28052150707062	37.0	35.0	37.0	33.0	37.0
7	35.85355370119365	37.0	35.0	37.0	35.0	37.0
8	35.82919928819369	37.0	35.0	37.0	35.0	37.0
9	37.71361042545539	39.0	38.0	39.0	35.0	39.0
10	37.274645239063126	39.0	37.0	39.0	34.0	39.0
11	37.23683023606993	39.0	37.0	39.0	34.0	39.0
12	37.21839426524977	39.0	37.0	39.0	34.0	39.0
13	37.164222712431894	39.0	37.0	39.0	33.0	39.0
14	38.291107224318566	40.0	38.0	41.0	34.0	41.0
15	38.33606254898354	40.0	38.0	41.0	34.0	41.0
16	38.40549646615581	40.0	38.0	41.0	34.0	41.0
17	38.35981715153297	40.0	38.0	41.0	34.0	41.0
18	38.34358243820605	40.0	37.0	41.0	34.0	41.0
19	38.27003771073907	40.0	37.0	41.0	34.0	41.0
20	38.24224141667731	40.0	37.0	41.0	34.0	41.0
21	38.223294669770375	40.0	37.0	41.0	34.0	41.0
22	38.27252715840971	40.0	37.0	41.0	34.0	41.0
23	38.275096268405804	40.0	37.0	41.0	34.0	41.0
24	38.23756359806608	40.0	37.0	41.0	34.0	41.0
25	38.112541778789435	40.0	37.0	41.0	34.0	41.0
26	38.13871671022712	40.0	37.0	41.0	34.0	41.0
27	38.109911750544455	40.0	37.0	41.0	34.0	41.0
28	38.0024074423356	40.0	37.0	41.0	34.0	41.0
29	37.96231503403807	40.0	37.0	41.0	34.0	41.0
30	37.83593661221903	40.0	36.0	41.0	33.0	41.0
31	37.81380220078889	40.0	37.0	41.0	33.0	41.0
32	37.74302573913166	40.0	37.0	41.0	33.0	41.0
33	37.60794537976085	40.0	36.0	41.0	33.0	41.0
34	37.560938750215264	40.0	36.0	41.0	33.0	41.0
35	37.49952964082835	40.0	36.0	41.0	33.0	41.0
36	37.47989522061192	40.0	36.0	41.0	33.0	41.0
37	37.40497046050682	40.0	36.0	41.0	32.0	41.0
38	37.35624874795426	40.0	36.0	41.0	32.0	41.0
39	37.31813969725973	40.0	36.0	41.0	32.0	41.0
40	37.20264104038997	40.0	36.0	41.0	32.0	41.0
41	37.183236235111636	40.0	36.0	41.0	32.0	41.0
42	37.20233410613599	40.0	36.0	41.0	32.0	41.0
43	37.10449705542272	39.0	36.0	41.0	32.0	41.0
44	37.00945638663407	39.0	35.0	41.0	31.0	41.0
45	36.96477636559384	39.0	35.0	41.0	31.0	41.0
46	36.83748651305115	39.0	35.0	41.0	31.0	41.0
47	36.694380174390204	39.0	35.0	41.0	31.0	41.0
48	36.69116907977128	39.0	35.0	41.0	31.0	41.0
49	36.71294618224163	39.0	35.0	41.0	31.0	41.0
50	36.58720505902627	39.0	35.0	41.0	31.0	41.0
51	35.372952063195655	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	14.0
10	19.0
11	19.0
12	6.0
13	7.0
14	14.0
15	21.0
16	28.0
17	61.0
18	149.0
19	257.0
20	617.0
21	1042.0
22	1643.0
23	2279.0
24	3149.0
25	4269.0
26	5410.0
27	6582.0
28	7534.0
29	9122.0
30	11548.0
31	14833.0
32	19991.0
33	27832.0
34	46328.0
35	63888.0
36	61189.0
37	93655.0
38	175705.0
39	296206.0
40	178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44654365085409	21.51632550494785	24.63170818284378	10.405422661354283
2	30.54440998918701	28.32909443851533	24.328288443222434	16.798207129075227
3	29.961000605667977	27.59666964619384	26.543721144372732	15.898608603765451
4	26.553093182662195	26.03458516429769	30.08061124433724	17.331710408702875
5	27.052037071097455	30.25422825364953	24.50916878220906	18.184565893043956
6	24.235153812720903	36.48007328933942	24.948951678942084	14.33582121899759
7	77.50839676055496	6.12450987168508	11.890656429276842	4.476436938483112
8	78.35094300277764	7.790038226201173	8.6438309143712	5.215187856649988
9	72.59815159974836	7.223147060167315	10.40214244795297	9.776558892131353
10	38.052232712396744	27.1689532487585	19.586857122104774	15.191956916739983
11	27.84866032570176	25.812233555880194	26.322892492177274	20.01621362624077
12	23.361796994621624	22.554981648377527	31.329083894972253	22.754137462028602
13	22.893429381105737	25.158651035668804	33.89233636714023	18.055583216085232
14	20.413939501149834	29.672810428267006	29.62044416432463	20.29280590625853
15	18.501340787227786	26.745102817117562	35.5865665889178	19.166989806736858
16	20.85676831032693	26.448360654777453	29.886024299352275	22.80884673554334
17	21.821033899833996	26.720501216607722	28.88766792056729	22.57079696299099
18	21.36566998944474	26.49838390914746	30.80682706129196	21.329119040115838
19	21.184438199022264	28.3135134248591	28.47002646429312	22.03202191182552
20	21.78893466869259	27.701870775993054	31.20033551897076	19.30885903634359
21	22.036590780491633	29.04160364947171	29.908634341725605	19.013171228311055
22	19.74360446249603	26.030016295631576	30.166716846121677	24.059662395750717
23	19.177884801248354	27.78797637777749	29.70725266898078	23.326886151993374
24	21.2840161058478	25.825237259006823	28.886496415781103	24.00425021936427
25	18.894849244906588	30.21310843565451	28.024034592193324	22.86800772724557
26	20.079006282780167	27.346787675301048	29.921520894373614	22.65268514754517
27	20.74078933649483	29.148327735492963	28.450696635321105	21.6601862926911
28	18.31788313771156	27.391304857175996	30.948930591855934	23.341881413256512
29	21.758006942337364	26.124908183312385	29.07007121577595	23.047013658574304
30	20.083926602882133	29.203622761400794	29.60158293726709	21.11086769844998
31	20.68443995627944	27.837413879754408	26.344096728807187	25.134049435158968
32	21.26656068453368	31.210527610610555	25.749675200298032	21.77323650455774
33	19.239623103480188	27.39165630861185	27.52028753413472	25.84843305377324
34	20.561431953730246	25.949065314906345	27.873027625254366	25.616475106109043
35	19.089904791806028	29.74122630778008	26.753654802056694	24.4152140983572
36	21.052995362012553	26.91157364723414	26.66122307442687	25.37420791632644
37	18.305113735542168	30.590684428241232	28.180899083063203	22.9233027531534
38	17.836394670590426	28.926093277554088	27.429847364641407	25.80766468721408
39	19.48493620570687	27.35077079157407	28.577687754143323	24.586605248575744
40	20.259769471288173	26.629592445199933	30.043240241658008	23.067397841853882
41	18.758486087794914	26.73432497308468	26.28399853327601	28.2231904058444
42	20.086035311497266	26.893415323048302	28.37548602804817	24.645063337406263
43	21.3493860729168	26.042434246365115	26.989361565036674	25.618818115681414
44	20.10770815004165	25.847613000422914	28.451399538192813	25.593279311342627
45	19.55030617277587	25.42821428696947	27.99861293833316	27.022866601921503
46	22.94860725653495	26.830388365551666	27.05894894933593	23.162055428577453
47	17.91265963217093	26.090583093077225	31.730675735675717	24.26608153907613
48	19.243137617838737	26.166496603221873	28.099713801380737	26.490651977558656
49	19.67835164590565	23.940754659953164	31.48020801238983	24.90068568175135
50	19.848454140859392	23.804508653320102	29.944130936746944	26.40290626907356
51	19.104197150197457	23.916738811836417	27.173756418381846	29.80530761958428
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	284.0
1	562.0
2	840.0
3	1878.0
4	2916.0
5	2096.0
6	1276.0
7	1256.5
8	1237.0
9	1230.5
10	1224.0
11	1330.0
12	1436.0
13	1428.5
14	1421.0
15	1438.0
16	1455.0
17	1505.5
18	1556.0
19	1770.5
20	1985.0
21	2133.5
22	2282.0
23	2939.5
24	3597.0
25	4138.5
26	6276.0
27	7872.0
28	9303.0
29	10734.0
30	12023.0
31	13312.0
32	16437.0
33	19562.0
34	21686.0
35	23810.0
36	27316.0
37	30822.0
38	32617.0
39	34412.0
40	39020.5
41	43629.0
42	48821.0
43	54013.0
44	60671.5
45	67330.0
46	90883.0
47	114436.0
48	107290.5
49	100145.0
50	96774.5
51	93404.0
52	78169.5
53	62935.0
54	53425.5
55	43916.0
56	37709.5
57	31503.0
58	28367.0
59	25231.0
60	21520.5
61	17810.0
62	15268.0
63	12726.0
64	10506.0
65	8286.0
66	6757.5
67	5229.0
68	3974.5
69	2720.0
70	2222.5
71	1725.0
72	1308.5
73	892.0
74	704.5
75	364.0
76	211.0
77	165.5
78	120.0
79	94.5
80	69.0
81	45.5
82	22.0
83	15.5
84	9.0
85	7.5
86	6.0
87	4.5
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	853603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.583364971990775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.48605104502016	22.57770040561234
2	12.210676311802054	7.713084930209729
3	4.582533270769298	4.341954623609921
4	2.2238137372186513	2.809420835692138
5	1.3059547412591552	2.062322261504484
6	0.9025065440427043	1.7102516142066477
7	0.6427881649415518	1.4210989249117665
8	0.49970588278721473	1.2625914619775556
9	0.42213423906772346	1.1999177765684568
>10	5.297202285147935	39.26437090920928
>50	0.3680835157583455	7.137614185371323
>100	0.05146456166603009	2.8543859453137634
>500	0.0014917264251023165	0.3855081785512886
>1k	0.004475179275306949	2.409867807259201
>5k	0.0011187948188267373	2.8499101400021325
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGC	9046	1.0597432295809646	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	8152	0.9550107016962218	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	6998	0.8198190493707261	No Hit
GCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	4360	0.5107760867757025	No Hit
CCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	3302	0.38683088039756186	No Hit
CTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGCT	1746	0.20454473566751757	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCT	1599	0.18732361531063035	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1438	0.1684623882530872	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATCACT	1335	0.15639588895540432	No Hit
GAACTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCT	1231	0.14421223917910317	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATCACTCGTA	1138	0.13331724466760309	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTT	1124	0.13167713796694716	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTC	1078	0.12628821595050627	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCC	1070	0.12535101212156002	No Hit
GTAGCAGGGAAGGACTTTTTTTTTATTTCTTCCCAAATAAGTGTTGACAGC	1039	0.12171934728439333	No Hit
TCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1000	0.1171504786182804	No Hit
CGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTG	987	0.11562752239624274	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5145143585484117E-4	0.0	0.0	0.2152054292217811	0.0
2	3.5145143585484117E-4	0.0	0.0	1.382141346738472	0.0
3	3.5145143585484117E-4	0.0	0.0	1.8794451284730724	0.0
4	3.5145143585484117E-4	0.0	0.0	2.580004990610389	0.0
5	3.5145143585484117E-4	0.0	0.0	4.814767520732706	0.0
6	3.5145143585484117E-4	0.0	0.0	5.779267411197009	0.0
7	3.5145143585484117E-4	0.0	0.0	6.795196361774736	0.0
8	3.5145143585484117E-4	0.0	0.0	8.251728262435817	0.0
9	4.686019144731216E-4	0.0	0.0	8.706154968996126	0.0
10	4.686019144731216E-4	0.0	0.0	10.790496284572571	0.0
11	4.686019144731216E-4	0.0	0.0	12.4575475953107	0.0
12	4.686019144731216E-4	0.0	0.0	14.840271179927905	0.0
13	4.686019144731216E-4	0.0	0.0	15.425086369190362	0.0
14	5.85752393091402E-4	0.0	0.0	15.672156728596315	0.0
15	5.85752393091402E-4	0.0	0.0	16.194179261319373	0.0
16	5.85752393091402E-4	0.0	0.0	17.06777038037589	0.0
17	5.85752393091402E-4	0.0	0.0	18.1572698315259	0.0
18	5.85752393091402E-4	0.0	0.0	19.317059569846872	0.0
19	5.85752393091402E-4	0.0	0.0	20.183387359229055	0.0
20	5.85752393091402E-4	0.0	0.0	20.893436410134452	0.0
21	5.85752393091402E-4	0.0	0.0	21.744183185860408	0.0
22	5.85752393091402E-4	0.0	0.0	22.673537932739226	0.0
23	5.85752393091402E-4	0.0	0.0	23.58742881644043	0.0
24	5.85752393091402E-4	0.0	0.0	24.33250586045269	0.0
25	5.85752393091402E-4	0.0	0.0	24.998154879961763	0.0
26	5.85752393091402E-4	0.0	0.0	25.62444133865509	0.0
27	5.85752393091402E-4	0.0	0.0	26.30016529932533	0.0
28	5.85752393091402E-4	0.0	0.0	26.93992406305976	0.0
29	5.85752393091402E-4	0.0	0.0	27.61717097995204	0.0
30	5.85752393091402E-4	0.0	0.0	28.36306807731463	0.0
31	8.200533503279628E-4	0.0	0.0	29.044063809522694	0.0
32	8.200533503279628E-4	0.0	0.0	29.704206756536703	0.0
33	8.200533503279628E-4	0.0	0.0	30.344316971706988	0.0
34	9.372038289462432E-4	0.0	0.0	30.98513008974898	0.0
35	9.372038289462432E-4	0.0	0.0	31.621842941039336	0.0
36	9.372038289462432E-4	0.0	0.0	32.24028031766524	0.0
37	9.372038289462432E-4	0.0	0.0	32.89023117303946	0.0
38	9.372038289462432E-4	0.0	0.0	33.50714559344332	0.0
39	9.372038289462432E-4	0.0	0.0	34.13846952271724	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	30	2.164843E-6	45.000004	1
ATCTCGC	40	6.8121153E-9	45.0	31
CATCTCG	25	3.889959E-5	45.0	30
CCGCTAT	20	7.032265E-4	45.0	43
CGTTCCT	20	7.032265E-4	45.0	29
TTATCCG	20	7.032265E-4	45.0	39
GTTCGGT	20	7.032265E-4	45.0	14
TCTCGCG	20	7.032265E-4	45.0	31
CGAATTG	20	7.032265E-4	45.0	14
ACGTCGT	20	7.032265E-4	45.0	39
TGTCGTC	20	7.032265E-4	45.0	17
TATCGAG	20	7.032265E-4	45.0	1
GCCCGTA	25	3.889959E-5	45.0	28
TCGAACG	25	3.889959E-5	45.0	1
TACGTAG	25	3.889959E-5	45.0	1
CCCGATA	25	3.889959E-5	45.0	11
ACGGTAA	20	7.032265E-4	45.0	11
CGTAAGG	155	0.0	42.09677	2
CGAACGG	70	0.0	41.785713	2
TTAGCGG	130	0.0	41.538464	2
>>END_MODULE