##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936451.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480426
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00359680783305	33.0	31.0	34.0	30.0	34.0
2	32.02752557105569	33.0	31.0	34.0	30.0	34.0
3	32.08998264040664	33.0	31.0	34.0	30.0	34.0
4	35.7755450371129	37.0	35.0	37.0	35.0	37.0
5	34.741506496317854	37.0	35.0	37.0	33.0	37.0
6	35.26205700773897	37.0	35.0	37.0	33.0	37.0
7	35.810041504831126	37.0	35.0	37.0	35.0	37.0
8	35.744351887699665	37.0	35.0	37.0	35.0	37.0
9	37.6173458555532	39.0	37.0	39.0	35.0	39.0
10	37.37114768975867	39.0	37.0	39.0	34.0	39.0
11	37.29624125255502	39.0	37.0	39.0	34.0	39.0
12	37.11048735913543	39.0	37.0	39.0	34.0	39.0
13	36.915778912881485	39.0	35.0	39.0	33.0	39.0
14	37.99876151582138	40.0	37.0	41.0	33.0	41.0
15	38.06682818998139	40.0	37.0	41.0	33.0	41.0
16	38.186430376374304	40.0	37.0	41.0	34.0	41.0
17	38.14066890634562	40.0	36.0	41.0	34.0	41.0
18	38.155255960335204	40.0	37.0	41.0	34.0	41.0
19	38.079414935911046	40.0	36.0	41.0	34.0	41.0
20	38.006077939162324	40.0	36.0	41.0	34.0	41.0
21	37.99692148218456	40.0	36.0	41.0	34.0	41.0
22	38.02745271904518	40.0	36.0	41.0	34.0	41.0
23	38.014093741804146	40.0	36.0	41.0	34.0	41.0
24	37.966854416705175	40.0	36.0	41.0	34.0	41.0
25	37.856304612989305	40.0	36.0	41.0	34.0	41.0
26	37.82072160957151	40.0	36.0	41.0	34.0	41.0
27	37.80642180065192	40.0	36.0	41.0	34.0	41.0
28	37.70184378031164	40.0	36.0	41.0	33.0	41.0
29	37.61487305016798	40.0	36.0	41.0	33.0	41.0
30	37.565085986187256	40.0	36.0	41.0	33.0	41.0
31	37.486237214472155	40.0	35.0	41.0	33.0	41.0
32	37.281418990645804	39.0	35.0	41.0	33.0	41.0
33	37.19230224842119	39.0	35.0	41.0	33.0	41.0
34	37.1182658723716	39.0	35.0	41.0	32.0	41.0
35	37.06406813952617	39.0	35.0	41.0	32.0	41.0
36	37.03954407130338	39.0	35.0	41.0	32.0	41.0
37	36.91905100889627	39.0	35.0	41.0	32.0	41.0
38	36.821722804344475	39.0	35.0	41.0	31.0	41.0
39	36.71973415260623	39.0	35.0	41.0	31.0	41.0
40	36.61038536632072	39.0	35.0	41.0	31.0	41.0
41	36.579839559058	39.0	35.0	41.0	31.0	41.0
42	36.621207012110084	39.0	35.0	41.0	31.0	41.0
43	36.53127849033982	39.0	35.0	41.0	31.0	41.0
44	36.39604018100602	39.0	35.0	41.0	31.0	41.0
45	36.37922801846695	39.0	35.0	41.0	31.0	41.0
46	36.204949357445265	38.0	35.0	40.0	30.0	41.0
47	36.060042545574134	38.0	35.0	40.0	30.0	41.0
48	36.05076536240753	38.0	35.0	40.0	30.0	41.0
49	36.05128989688318	38.0	35.0	40.0	30.0	41.0
50	35.922400119893595	38.0	35.0	40.0	30.0	41.0
51	34.66632530296029	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	4.0
9	4.0
10	9.0
11	9.0
12	5.0
13	3.0
14	13.0
15	13.0
16	19.0
17	49.0
18	112.0
19	231.0
20	449.0
21	788.0
22	1069.0
23	1522.0
24	1924.0
25	2581.0
26	3360.0
27	4207.0
28	4826.0
29	5789.0
30	7355.0
31	9515.0
32	12910.0
33	17769.0
34	32486.0
35	46355.0
36	35011.0
37	52582.0
38	93101.0
39	146259.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.15112421059643	20.506383917606456	22.96815742695025	10.374334444846864
2	30.55184357216304	29.495905717009485	22.635119664630974	17.3171310461965
3	30.483570830887587	28.511362832153132	24.59296540986541	16.41210092709387
4	28.714307718566438	23.865277899197796	28.88041030252318	18.54000407971259
5	27.645880947325914	30.127220425205962	22.49420306144963	19.732695566018492
6	24.889785315532464	35.141728382726996	24.545299380133464	15.423186921607074
7	72.91341434476901	6.963195164291691	14.38181946855499	5.741571022384301
8	70.91060850162148	10.735472268361828	11.552039231848402	6.8018799981682925
9	64.87138497916433	9.16873774525109	13.229508810930298	12.730368464654285
10	40.7455050309517	24.004321164966093	20.425414111642585	14.824759692439626
11	33.22592865498537	23.598431392139478	24.417079841640543	18.758560111234612
12	29.39474549670501	20.793837136208282	29.244254057856985	20.567163309229723
13	24.396681278698487	26.32621881413579	31.166090095040648	18.111009812125072
14	19.188386973227928	31.312002264656787	28.323196496442744	21.17641426567255
15	16.537614533767947	25.688867796497277	37.99794349181768	19.7755741779171
16	18.65802433673448	25.73382789441038	31.61485847976587	23.99328928908927
17	18.826208406705717	24.74095906549604	29.67054239362566	26.762290134172588
18	20.59234929000512	25.240307560373505	30.75229067535895	23.415052474262428
19	22.04314504210846	27.407967095869086	27.59883936339832	22.950048498624138
20	24.97970551135867	25.697818186359605	30.33578532385841	18.986690978423315
21	22.311240440775478	29.04963511550166	28.60752748602282	20.03159695770004
22	20.0494977374247	25.35832781739539	28.654777218551867	25.937397226628033
23	20.599426342454404	28.02533584776844	28.359206204493514	23.016031605283644
24	22.496284547464125	24.16667707409674	29.70530321006773	23.631735168371403
25	18.900517457423206	29.932185185647736	26.98147061149896	24.1858267454301
26	18.472355784241486	28.63354606120401	28.95159712421892	23.942501030335578
27	22.837440105239935	28.289892720210812	27.31263503640519	21.560032138144063
28	17.66744514243609	28.040114398471356	31.916257654664822	22.37618280442774
29	20.60671154350514	26.641147648128953	30.43548850395274	22.316652304413168
30	23.010619741645954	27.71186405398542	29.070241827045162	20.207274377323458
31	27.764733798753603	27.233538567854364	23.521416409603145	21.48031122378889
32	24.534267504256636	30.194244274872716	25.397667903069358	19.873820317801286
33	22.75605400207316	28.683918022754806	24.548213460553757	24.011814514618276
34	23.2431217294651	27.64837873054331	27.50517249274602	21.603327047245568
35	21.785665222115373	28.081744118761264	26.349531457498138	23.78305920162522
36	24.908310541061475	29.295250465212124	24.360255273444817	21.436183720281583
37	24.315295175531716	26.478167293193955	29.8580842835317	19.34845324774263
38	21.878083201158972	29.57729182017626	24.884789749097678	23.65983522956709
39	23.64693001627722	25.240723857576402	28.510530237747332	22.601815888399045
40	22.74106730276879	28.231611111804938	27.265385303876144	21.761936281550124
41	21.480519372390336	27.13820650839047	25.43929762335927	25.941976495859926
42	22.12224151065929	26.087264219671706	27.801159804007273	23.989334465661724
43	24.19248750067648	24.3463093171477	26.24837123719366	25.212831944982163
44	22.5066919775366	25.298381020177924	28.13503016073235	24.059896841553122
45	21.831249765832826	25.04173379459063	27.021018845774375	26.105997593802165
46	24.717021976329338	27.397767814398055	25.947388359497616	21.93782184977499
47	20.745546660671987	25.289846927518493	31.853813074229954	22.110793337579565
48	22.148676383043384	24.90622905504698	27.559082980521453	25.386011581388185
49	21.241148480723357	22.570177300978713	32.006594147693924	24.182080070604005
50	21.528601699325183	22.66093009121072	30.191746491655323	25.61872171780878
51	20.04491846819281	23.990583357270424	26.286046134056022	29.678452040480742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	350.0
1	585.0
2	820.0
3	1559.0
4	2298.0
5	1599.0
6	900.0
7	882.0
8	864.0
9	936.5
10	1009.0
11	1009.0
12	1009.0
13	977.5
14	946.0
15	1075.0
16	1204.0
17	1180.0
18	1156.0
19	1306.5
20	1457.0
21	1666.0
22	1875.0
23	2014.0
24	2153.0
25	2310.0
26	3429.0
27	4391.0
28	5114.0
29	5837.0
30	6448.5
31	7060.0
32	7974.5
33	8889.0
34	10407.5
35	11926.0
36	12703.0
37	13480.0
38	14551.5
39	15623.0
40	18965.0
41	22307.0
42	24347.0
43	26387.0
44	30275.5
45	34164.0
46	48586.0
47	63008.0
48	57969.0
49	52930.0
50	52083.0
51	51236.0
52	42277.5
53	33319.0
54	28983.5
55	24648.0
56	22036.0
57	19424.0
58	18296.5
59	17169.0
60	16486.5
61	15804.0
62	13598.0
63	11392.0
64	10017.0
65	8642.0
66	7047.0
67	5452.0
68	4429.5
69	3407.0
70	2812.5
71	2218.0
72	1763.0
73	1308.0
74	1076.0
75	652.0
76	460.0
77	356.0
78	252.0
79	217.5
80	183.0
81	153.5
82	124.0
83	70.0
84	16.0
85	9.5
86	3.0
87	6.0
88	9.0
89	6.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	480426.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.21870674825126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.22355830699821	20.81060263739156
2	12.184312449739023	7.120197047959829
3	4.831078810113749	4.234736250912128
4	2.4164906966328914	2.8242693209917547
5	1.4397029039344131	2.1033128477332688
6	0.8831487742687667	1.5482679030261988
7	0.6581856225845059	1.34619328845483
8	0.4565803430488812	1.0672549720448976
9	0.33735994194268826	0.8871499091007431
>10	4.765882918922355	34.54766747111419
>50	0.7159840538968504	13.014028298741545
>100	0.07630507347307071	3.8968947794973245
>500	0.004991920694499953	1.0212106359916793
>1k	0.004991920694499953	3.1255171474954477
>5k	0.0014262630555714152	2.4526974895445948
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC	6272	1.3055080282915579	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG	5499	1.1446091593710583	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC	4617	0.9610220928925578	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	3351	0.6975059634574315	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	2026	0.421709066536782	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT	1635	0.3403229633700091	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1151	0.23957904026842844	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCT	1136	0.23645681124668522	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTAGACT	1084	0.22563308397130882	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTA	948	0.19732487417417044	No Hit
TCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	819	0.1704737045871789	TruSeq Adapter, Index 15 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCT	752	0.15652774829005925	No Hit
GAAGAAGGAGAGGAATACTAAATTAAATGTCACAAGGTGCTGCTTCCACAG	662	0.13779437415960002	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTC	655	0.1363373339494532	No Hit
ACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	556	0.11573062240594804	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC	509	0.10594763813781935	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0814860144954686E-4	0.0	0.0	0.3663415385512025	0.0
2	2.0814860144954686E-4	0.0	0.0	1.8945685703937756	0.0
3	2.0814860144954686E-4	0.0	0.0	2.4750950198365618	0.0
4	2.0814860144954686E-4	0.0	0.0	3.2864582682868955	0.0
5	2.0814860144954686E-4	0.0	0.0	5.9859374804860686	0.0
6	2.0814860144954686E-4	0.0	0.0	6.929266942255415	0.0
7	2.0814860144954686E-4	0.0	0.0	8.106347283452603	0.0
8	2.0814860144954686E-4	0.0	0.0	9.59044681178787	0.0
9	2.0814860144954686E-4	0.0	0.0	10.090003455266784	0.0
10	2.0814860144954686E-4	0.0	0.0	12.369646938342221	0.0
11	2.0814860144954686E-4	0.0	0.0	14.097904776177808	0.0
12	2.0814860144954686E-4	0.0	0.0	16.86732191846403	0.0
13	2.0814860144954686E-4	0.0	0.0	17.55109007422579	0.0
14	2.0814860144954686E-4	0.0	0.0	17.866018908218955	0.0
15	2.0814860144954686E-4	0.0	0.0	18.570601924125672	0.0
16	2.0814860144954686E-4	0.0	0.0	19.579914492554526	0.0
17	2.0814860144954686E-4	0.0	0.0	20.727854029548777	0.0
18	2.0814860144954686E-4	0.0	0.0	22.055217660992536	0.0
19	2.0814860144954686E-4	0.0	0.0	23.062448743406893	0.0
20	2.0814860144954686E-4	0.0	0.0	23.76120359847302	0.0
21	2.0814860144954686E-4	0.0	0.0	24.60358098853934	0.0
22	2.0814860144954686E-4	0.0	0.0	25.4469991216129	0.0
23	2.0814860144954686E-4	0.0	0.0	26.27064313754876	0.0
24	2.0814860144954686E-4	0.0	0.0	26.938800148201803	0.0
25	2.0814860144954686E-4	0.0	0.0	27.546177767231583	0.0
26	4.1629720289909373E-4	0.0	0.0	28.090070062819247	0.0
27	4.1629720289909373E-4	0.0	0.0	28.679546902124365	0.0
28	4.1629720289909373E-4	0.0	0.0	29.247792584081626	0.0
29	4.1629720289909373E-4	0.0	0.0	29.838726463596892	0.0
30	4.1629720289909373E-4	0.0	0.0	30.488982694525276	0.0
31	4.1629720289909373E-4	0.0	0.0	31.115093687685512	0.0
32	4.1629720289909373E-4	0.0	0.0	31.70810905321527	0.0
33	4.1629720289909373E-4	0.0	0.0	32.30154071594793	0.0
34	4.1629720289909373E-4	0.0	0.0	32.8887279206371	0.0
35	4.1629720289909373E-4	0.0	0.0	33.51254927918139	0.0
36	6.244458043486406E-4	0.0	0.0	34.126587653457555	0.0
37	6.244458043486406E-4	0.0	0.0	34.7039918738786	0.0
38	6.244458043486406E-4	0.0	0.0	35.290554632763424	0.0
39	6.244458043486406E-4	0.0	0.0	35.85775957171344	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.029181E-4	45.000004	25
CTGCTAT	20	7.029181E-4	45.000004	41
GAATGCG	20	7.029181E-4	45.000004	1
CAACGCC	20	7.029181E-4	45.000004	14
ACGCAAC	20	7.029181E-4	45.000004	22
GTTTACG	20	7.029181E-4	45.000004	1
GCAACGA	20	7.029181E-4	45.000004	11
ACACCCG	20	7.029181E-4	45.000004	10
ATCAGTC	20	7.029181E-4	45.000004	6
ATTCGAC	20	7.029181E-4	45.000004	13
TGACGTA	20	7.029181E-4	45.000004	26
CGTTCGA	20	7.029181E-4	45.000004	21
CCGCTAT	20	7.029181E-4	45.000004	26
ACCTAGC	20	7.029181E-4	45.000004	36
TTGACGA	20	7.029181E-4	45.000004	22
CGGTCTA	20	7.029181E-4	45.000004	31
GACGCAA	20	7.029181E-4	45.000004	21
AGTAGGC	20	7.029181E-4	45.000004	41
GTAAGCA	20	7.029181E-4	45.000004	29
CGGTAAC	20	7.029181E-4	45.000004	6
>>END_MODULE