FastQCFastQC Report
Sat 14 Jan 2017
SRR2936450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488480
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157651.0591341502741052No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC62180.41774158873481676No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG40180.2699398043641836No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC40040.2689992475545523No Hit
CGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG28470.19126894550145115No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTT26770.17984789852735678No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC26720.1795119853810599TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC19400.13033430076319466TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC18570.12475814253466624No Hit
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT16770.11266526926797807TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT16280.1093733204342685No Hit
GAACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT15690.10540954530796519No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA207.033958E-445.013
TAATGCG453.8562575E-1045.01
CGTTTTT78250.042.642171
CGCGTAA501.0822987E-940.531
TATAGCG800.039.3751
ACAACGA3550.038.66197213
ACGTACG356.249531E-638.571431
CGTAAGG1700.038.382352
GCGTAAG950.037.8947371
CCGACCG601.5643309E-1037.50000415
ATTAGCG1000.036.01
CACGCTA250.002107307836.030
TACGCTA250.002107307836.015
CGTCCGA250.002107307836.036
CGATCGG458.702227E-735.02
GTTTTTT101000.034.3960382
ATAGCGG2300.034.239132
CTACGGG4350.034.137933
ATAGGGC7200.034.06254
ACGGGTA1850.034.0540545
Produced by FastQC (version 0.11.5)