##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936450.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1488480
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18460845963668	33.0	31.0	34.0	30.0	34.0
2	32.2488659572181	34.0	31.0	34.0	30.0	34.0
3	32.220266983768674	34.0	31.0	34.0	30.0	34.0
4	35.87582634633989	37.0	35.0	37.0	35.0	37.0
5	34.96142776523702	37.0	35.0	37.0	33.0	37.0
6	35.429924486724715	37.0	35.0	37.0	33.0	37.0
7	35.95221433946039	37.0	35.0	37.0	35.0	37.0
8	35.93635520799742	37.0	35.0	37.0	35.0	37.0
9	37.810100908309145	39.0	38.0	39.0	35.0	39.0
10	37.515863834247014	39.0	37.0	39.0	35.0	39.0
11	37.44078724604966	39.0	37.0	39.0	35.0	39.0
12	37.37523245189724	39.0	37.0	39.0	35.0	39.0
13	37.34992206815006	39.0	37.0	39.0	35.0	39.0
14	38.542815489627	40.0	38.0	41.0	34.0	41.0
15	38.62413603138772	40.0	38.0	41.0	35.0	41.0
16	38.61747823282812	40.0	38.0	41.0	35.0	41.0
17	38.57976459206707	40.0	38.0	41.0	34.0	41.0
18	38.49608661184564	40.0	38.0	41.0	34.0	41.0
19	38.34799862947436	40.0	37.0	41.0	34.0	41.0
20	38.27142252499194	40.0	37.0	41.0	34.0	41.0
21	38.18278646673116	40.0	37.0	41.0	34.0	41.0
22	38.21915175212297	40.0	37.0	41.0	34.0	41.0
23	38.20491373750403	40.0	37.0	41.0	34.0	41.0
24	38.16451279157261	40.0	36.0	41.0	34.0	41.0
25	38.017424486724714	40.0	36.0	41.0	34.0	41.0
26	38.038426448457486	40.0	36.0	41.0	34.0	41.0
27	38.03812278297323	40.0	36.0	41.0	34.0	41.0
28	37.912750591207136	40.0	36.0	41.0	34.0	41.0
29	37.85252270772869	40.0	36.0	41.0	34.0	41.0
30	37.71093397291197	40.0	36.0	41.0	33.0	41.0
31	37.64345641191014	40.0	36.0	41.0	33.0	41.0
32	37.485830511662904	40.0	36.0	41.0	33.0	41.0
33	37.25362383102225	40.0	35.0	41.0	33.0	41.0
34	37.06385440180587	40.0	35.0	41.0	32.0	41.0
35	36.929533483822425	40.0	35.0	41.0	31.0	41.0
36	36.829701440395574	40.0	35.0	41.0	31.0	41.0
37	36.78325674513598	40.0	35.0	41.0	31.0	41.0
38	36.68736496291519	40.0	35.0	41.0	31.0	41.0
39	36.59223637536279	40.0	35.0	41.0	31.0	41.0
40	36.46797807158981	39.0	35.0	41.0	30.0	41.0
41	36.366622326131356	39.0	35.0	41.0	30.0	41.0
42	36.35566752660432	39.0	35.0	41.0	30.0	41.0
43	36.25527853918091	39.0	35.0	41.0	30.0	41.0
44	36.12836719337848	39.0	35.0	41.0	30.0	41.0
45	36.08207701816618	39.0	35.0	41.0	30.0	41.0
46	35.96207809308825	39.0	35.0	41.0	29.0	41.0
47	35.87314777491132	39.0	35.0	41.0	29.0	41.0
48	35.80940556809631	39.0	35.0	41.0	29.0	41.0
49	35.779254675911	38.0	35.0	41.0	29.0	41.0
50	35.67125456841879	38.0	35.0	40.0	28.0	41.0
51	34.56489170160163	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	13.0
9	37.0
10	48.0
11	37.0
12	30.0
13	34.0
14	48.0
15	68.0
16	103.0
17	187.0
18	354.0
19	783.0
20	1331.0
21	2204.0
22	3358.0
23	4881.0
24	7316.0
25	11505.0
26	16327.0
27	19161.0
28	19783.0
29	20134.0
30	22036.0
31	25757.0
32	32787.0
33	45908.0
34	82119.0
35	106614.0
36	106344.0
37	158000.0
38	275576.0
39	525236.0
40	357.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.24939535633666	20.581600021498442	22.888517145006986	15.280487477157905
2	36.004447490056975	24.445743308610126	22.95637160055896	16.593437600773946
3	30.70205847576051	23.7890331075997	28.811136192626037	16.69777222401376
4	26.761058260776093	24.627203590239706	29.96640868537031	18.645329463613887
5	25.343303235515425	29.09552026228098	27.037716328066214	18.523460174137373
6	25.53067558851983	33.215360636353864	27.064119101365154	14.18984467376115
7	75.6031656454907	6.298573040954531	13.310625604643663	4.787635708911104
8	76.55165000537461	6.491185639041169	12.29993013006557	4.65723422551865
9	69.98878050091368	7.59419004622165	14.502445447705043	7.914584005159626
10	38.70639847361066	24.927241212512094	21.48560948081264	14.880750833064601
11	28.703643985811027	25.622447060088145	27.57584918843384	18.09805976566699
12	25.324089003547247	23.010856712888316	31.55151564011609	20.11353864344835
13	24.56835160700849	22.637724389981724	33.01508921853166	19.778834784478125
14	20.569574330861013	26.000618080189184	32.893690207459954	20.53611738148984
15	19.755320864237344	25.026873051703753	34.92992851768247	20.287877566376437
16	22.696374825325165	25.32476082983984	31.581277544877995	20.397586799957004
17	22.725733634311513	25.356403848221003	30.577770611630655	21.34009190583683
18	24.26025206922498	24.933287649145434	30.397049338922926	20.409410942706653
19	23.472804471675804	26.013449962377727	29.624852198215628	20.88889336773084
20	25.227346017413737	25.606591959582932	29.996372138019993	19.169689884983338
21	24.69929055143502	26.240997527679244	30.355866387186932	18.703845533698807
22	24.500967429861333	23.511501666129206	30.346662366978393	21.640868537031068
23	22.900274105127377	25.36554068580028	30.904546920348274	20.829638288724066
24	22.45478609050844	23.899615715360635	31.643555842201444	22.002042351929486
25	22.34776416209825	26.392292808771362	29.487127808233904	21.772815220896486
26	21.78611738148984	25.890371385574547	30.130535848650975	22.192975384284637
27	22.65035472428249	25.065570246157154	30.43776201225411	21.846313017306247
28	20.784625927120285	25.855906696764485	31.206062560464364	22.153404815650866
29	21.645974416854777	25.14585348812211	31.49111845641191	21.7170536386112
30	23.46326453832097	24.737450284854347	30.820837364291087	20.97844781253359
31	23.621815543373106	25.104737719015368	28.92588412340105	22.34756261421047
32	23.39957540578308	25.814522197140704	29.207916801031924	21.577985596044286
33	23.946509190583683	25.334771041599485	28.696052348704722	22.022667419112114
34	22.23079920455767	24.906145866924646	30.188716005589594	22.67433892292809
35	22.260964205095128	25.729401805869074	30.345318714393205	21.664315274642586
36	23.055331613458023	26.132833494571646	29.841784908094166	20.97004998387617
37	22.992583037729762	26.28500214984414	29.000456841878965	21.721957970547134
38	21.823067827582502	27.165228958400515	28.554162635708913	22.457540578308073
39	22.734064280339673	25.079880146189403	29.086652155218744	23.099403418252177
40	23.573175319789318	24.491024400730947	30.65805385359561	21.27774642588412
41	21.44368752015479	24.96540094593142	30.435209072342257	23.155702461571536
42	22.06942652907664	25.02062506718263	30.4971514565194	22.412796947221324
43	22.61589003547243	24.44130925507901	30.19341878963775	22.749381919810812
44	22.54756530151564	24.40435880898635	29.402612060625604	23.64546382887241
45	22.054243254864023	23.980033322584113	29.0050252606686	24.96069816188326
46	22.943875631516715	24.957271847791034	28.918292486294746	23.18056003439751
47	21.47042620660002	24.85421369450715	31.01976512952811	22.65559496936472
48	21.147680855637965	24.59435128453187	30.995176287219177	23.262791572610986
49	21.984977964097602	23.953832097172956	31.082244974739332	22.97894496399011
50	20.963466086208747	24.253399441040525	31.657932924862948	23.125201547887777
51	21.043749328173707	23.538643448349994	30.250591207137482	25.167016016338817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	596.0
1	1201.5
2	1807.0
3	13904.5
4	26002.0
5	16918.0
6	7834.0
7	7562.5
8	7291.0
9	7332.5
10	7374.0
11	7194.5
12	7015.0
13	6761.5
14	6508.0
15	6250.5
16	5993.0
17	5842.5
18	5692.0
19	5686.5
20	5681.0
21	6071.0
22	6461.0
23	7104.5
24	7748.0
25	8722.0
26	11545.5
27	13395.0
28	16589.5
29	19784.0
30	22228.5
31	24673.0
32	28425.5
33	32178.0
34	36885.0
35	41592.0
36	45129.5
37	48667.0
38	52174.0
39	55681.0
40	60584.5
41	65488.0
42	69675.0
43	73862.0
44	81075.5
45	88289.0
46	109169.5
47	130050.0
48	127025.5
49	124001.0
50	122935.0
51	121869.0
52	109495.5
53	97122.0
54	89106.5
55	81091.0
56	77498.5
57	73906.0
58	68617.5
59	63329.0
60	60955.0
61	58581.0
62	54589.0
63	50597.0
64	44957.0
65	39317.0
66	33510.0
67	27703.0
68	23569.5
69	19436.0
70	16534.0
71	13632.0
72	10969.0
73	8306.0
74	6570.5
75	3863.5
76	2892.0
77	2190.0
78	1488.0
79	975.5
80	463.0
81	363.0
82	263.0
83	207.0
84	151.0
85	106.0
86	61.0
87	55.5
88	50.0
89	32.0
90	14.0
91	10.5
92	7.0
93	5.5
94	4.0
95	3.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1488480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.581142524334467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50071839047084	19.80314213702411
2	8.901338616877718	4.732155008651776
3	3.2205915675590036	2.568210104098669
4	1.607948768418765	1.7096446154066909
5	1.0883422068523714	1.446468965779579
6	0.7931137753868291	1.2649122180942178
7	0.6574434891213032	1.223291936022037
8	0.5056492371293504	1.0752587551565007
9	0.4624167643269563	1.1062409326394766
>10	7.589883691481478	48.85628449162513
>50	0.6177191293752486	9.98380306025327
>100	0.04759208869166312	2.127831363843747
>500	0.003362484500375987	0.5978058618018673
>1k	0.003362484500375987	1.9935570751934382
>5k	2.5865265387507594E-4	0.42750510048119733
>10k+	2.5865265387507594E-4	1.0838883739282852
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15765	1.0591341502741052	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC	6218	0.41774158873481676	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG	4018	0.2699398043641836	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC	4004	0.2689992475545523	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG	2847	0.19126894550145115	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTT	2677	0.17984789852735678	No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	2672	0.1795119853810599	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	1940	0.13033430076319466	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC	1857	0.12475814253466624	No Hit
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT	1677	0.11266526926797807	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT	1628	0.1093733204342685	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT	1569	0.10540954530796519	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0154788777813608E-4	0.0	0.0	0.11884607115984092	0.0
2	2.0154788777813608E-4	0.0	0.0	0.5205981941309256	0.0
3	2.0154788777813608E-4	0.0	0.0	0.8932602386326991	0.0
4	2.0154788777813608E-4	0.0	0.0	1.3456008814360958	0.0
5	2.0154788777813608E-4	0.0	0.0	2.5629501236160377	0.0
6	2.6873051703751476E-4	0.0	0.0	3.513517145006987	0.0
7	2.6873051703751476E-4	0.0	0.0	4.157597011716651	0.0
8	2.6873051703751476E-4	0.0	0.0	4.991467806084059	0.0
9	2.6873051703751476E-4	0.0	0.0	5.386501666129206	0.0
10	2.6873051703751476E-4	0.0	0.0	6.295684187896377	0.0
11	3.3591314629689346E-4	0.0	0.0	7.113699881758572	0.0
12	4.0309577555627216E-4	0.0	0.0	8.023822960335377	0.0
13	4.0309577555627216E-4	0.0	0.0	8.356578523057077	0.0
14	4.0309577555627216E-4	0.0	0.0	8.526013114049231	0.0
15	4.0309577555627216E-4	0.0	0.0	8.785002149844136	0.0
16	4.0309577555627216E-4	0.0	0.0	9.217523917016017	0.0
17	4.0309577555627216E-4	0.0	0.0	9.667378802536817	0.0
18	4.7027840481565086E-4	0.0	0.0	10.136044824250241	0.0
19	4.7027840481565086E-4	0.0	0.0	10.48337901752123	0.0
20	4.7027840481565086E-4	0.0	0.0	10.813783188218855	0.0
21	4.7027840481565086E-4	0.0	0.0	11.188595076856927	0.0
22	4.7027840481565086E-4	0.0	0.0	11.633276899924756	0.0
23	4.7027840481565086E-4	0.0	0.0	12.024951628506933	0.0
24	4.7027840481565086E-4	0.0	0.0	12.376518327421262	0.0
25	4.7027840481565086E-4	0.0	0.0	12.680721272707729	0.0
26	4.7027840481565086E-4	0.0	0.0	12.985192948511234	0.0
27	4.7027840481565086E-4	0.0	0.0	13.287649145436955	0.0
28	4.7027840481565086E-4	0.0	0.0	13.586074384607116	0.0
29	4.7027840481565086E-4	0.0	0.0	13.913052241212512	0.0
30	4.7027840481565086E-4	0.0	0.0	14.323941201762873	0.0
31	5.374610340750295E-4	0.0	0.0	14.664489949478662	0.0
32	5.374610340750295E-4	0.0	0.0	15.004434053531119	0.0
33	6.046436633344082E-4	0.0	0.0	15.363458024293239	0.0
34	7.390089218531656E-4	0.0	0.0	15.710120391271634	0.0
35	7.390089218531656E-4	0.0	0.0	16.069816188326346	0.0
36	7.390089218531656E-4	0.0	0.0	16.400825002687306	0.0
37	8.061915511125443E-4	0.0	0.0	16.73754434053531	0.0
38	8.061915511125443E-4	0.0	0.0	17.093276362463723	0.0
39	8.061915511125443E-4	0.0	0.0	17.48481672578738	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGA	20	7.033958E-4	45.0	13
TAATGCG	45	3.8562575E-10	45.0	1
CGTTTTT	7825	0.0	42.64217	1
CGCGTAA	50	1.0822987E-9	40.5	31
TATAGCG	80	0.0	39.375	1
ACAACGA	355	0.0	38.661972	13
ACGTACG	35	6.249531E-6	38.57143	1
CGTAAGG	170	0.0	38.38235	2
GCGTAAG	95	0.0	37.894737	1
CCGACCG	60	1.5643309E-10	37.500004	15
ATTAGCG	100	0.0	36.0	1
CACGCTA	25	0.0021073078	36.0	30
TACGCTA	25	0.0021073078	36.0	15
CGTCCGA	25	0.0021073078	36.0	36
CGATCGG	45	8.702227E-7	35.0	2
GTTTTTT	10100	0.0	34.396038	2
ATAGCGG	230	0.0	34.23913	2
CTACGGG	435	0.0	34.13793	3
ATAGGGC	720	0.0	34.0625	4
ACGGGTA	185	0.0	34.054054	5
>>END_MODULE