FastQCFastQC Report
Sat 14 Jan 2017
SRR2936449.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936449.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434546
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62860.4381874126030117No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC35520.2476044685914568No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC34400.23979712048271717No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC30210.21058927354020018No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG28220.19671728895413598No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC26250.18298472129858506No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG25580.1783142541263926No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT21060.14680602783040766No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG15880.11069704282748688No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC14600.1017743592746416No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14450.10072873229579253No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC207.033872E-445.00000445
TCGTCGT253.8912916E-545.022
CGGTCTA750.045.031
TCGAATT302.1658798E-644.99999636
CGTTTTT35000.040.9499971
CGCATCG403.4590266E-739.37500421
AATTGCG403.4590266E-739.3750041
CGTAAGG2050.038.4146352
TCGTAAG659.094947E-1238.0769271
TAGCGAT301.14006325E-437.4999961
TAACGCC601.5643309E-1037.49999612
CGTACGA301.14006325E-437.49999615
GCGAGAC2350.037.34042721
TCGTCCC1700.037.05882338
ATTGGGA8200.037.0426864
GTCGACG250.002107282436.01
GGTCGAT250.002107282436.015
TCGATTG751.8189894E-1236.01
CGGATTA250.002107282436.026
TAGTCGA250.002107282436.044
Produced by FastQC (version 0.11.5)