##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936449.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1434546
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07323292525998	33.0	31.0	34.0	30.0	34.0
2	32.11636434105285	33.0	31.0	34.0	30.0	34.0
3	32.13285457559395	33.0	31.0	34.0	30.0	34.0
4	35.791211296117375	37.0	35.0	37.0	35.0	37.0
5	34.8421953705214	37.0	35.0	37.0	33.0	37.0
6	35.32626559204097	37.0	35.0	37.0	33.0	37.0
7	35.9017138523268	37.0	35.0	37.0	35.0	37.0
8	35.90024439787919	37.0	35.0	37.0	35.0	37.0
9	37.7437593496479	39.0	38.0	39.0	35.0	39.0
10	37.41830934665044	39.0	37.0	39.0	35.0	39.0
11	37.33401438503889	39.0	37.0	39.0	34.0	39.0
12	37.16142249882541	39.0	37.0	39.0	34.0	39.0
13	37.05025143843418	39.0	37.0	39.0	33.0	39.0
14	37.94478253050094	40.0	37.0	41.0	33.0	41.0
15	38.11933740709604	40.0	37.0	41.0	33.0	41.0
16	38.18385259169103	40.0	37.0	41.0	33.0	41.0
17	38.20079244583304	40.0	37.0	41.0	34.0	41.0
18	38.17939961493044	40.0	37.0	41.0	34.0	41.0
19	38.09805750390716	40.0	37.0	41.0	34.0	41.0
20	38.0824072563724	40.0	36.0	41.0	34.0	41.0
21	37.97996648416991	40.0	36.0	41.0	33.0	41.0
22	38.05633350202782	40.0	36.0	41.0	34.0	41.0
23	38.06834636184549	40.0	36.0	41.0	34.0	41.0
24	38.03018934213333	40.0	36.0	41.0	34.0	41.0
25	37.869485537584715	40.0	36.0	41.0	34.0	41.0
26	37.90957069344587	40.0	36.0	41.0	34.0	41.0
27	37.90587265936401	40.0	36.0	41.0	34.0	41.0
28	37.78067974118641	40.0	36.0	41.0	34.0	41.0
29	37.73483318067179	40.0	35.0	41.0	33.0	41.0
30	37.59333893789394	40.0	35.0	41.0	33.0	41.0
31	37.51858497392206	40.0	35.0	41.0	33.0	41.0
32	37.412865812598554	40.0	35.0	41.0	33.0	41.0
33	37.25736225955808	40.0	35.0	41.0	32.0	41.0
34	37.135295766047236	40.0	35.0	41.0	32.0	41.0
35	37.06396727605807	40.0	35.0	41.0	32.0	41.0
36	36.988749053707586	39.0	35.0	41.0	32.0	41.0
37	36.96559190154934	39.0	35.0	41.0	32.0	41.0
38	36.84674105954079	39.0	35.0	41.0	31.0	41.0
39	36.78269849834024	39.0	35.0	41.0	31.0	41.0
40	36.644078335584915	39.0	35.0	41.0	31.0	41.0
41	36.55011759818089	39.0	35.0	41.0	31.0	41.0
42	36.582756495783336	39.0	35.0	41.0	31.0	41.0
43	36.498074652189615	39.0	35.0	41.0	31.0	41.0
44	36.38096791598178	39.0	35.0	41.0	31.0	41.0
45	36.30324855389789	39.0	35.0	41.0	31.0	41.0
46	36.18698250178105	38.0	35.0	41.0	30.0	41.0
47	36.079162327314705	38.0	35.0	40.0	30.0	41.0
48	36.012155762171446	38.0	35.0	40.0	30.0	41.0
49	35.99082636597223	38.0	35.0	40.0	30.0	41.0
50	35.87665644740566	38.0	35.0	40.0	30.0	41.0
51	34.737001113941275	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	29.0
10	28.0
11	35.0
12	30.0
13	44.0
14	38.0
15	72.0
16	104.0
17	203.0
18	370.0
19	651.0
20	1198.0
21	1902.0
22	2852.0
23	4288.0
24	6253.0
25	8910.0
26	11716.0
27	14043.0
28	15390.0
29	18123.0
30	21909.0
31	28129.0
32	36578.0
33	54273.0
34	99491.0
35	111723.0
36	102660.0
37	156779.0
38	274945.0
39	461476.0
40	292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58500877629577	21.348147776369668	23.181689538014115	11.88515390932044
2	33.198935412318605	24.672753609852872	24.01435715550425	18.113953822324273
3	32.48156559636289	24.95953423591854	25.89781017827243	16.661089989446136
4	28.704970074155867	25.237392178431367	26.847936559719937	19.209701187692833
5	25.27224641105967	30.44593899393955	25.43675838906525	18.84505620593554
6	26.36778465103245	33.496102599707505	25.732531407149022	14.403581342111025
7	78.45450755848888	6.144522378508601	10.498931369227616	4.9020386937748945
8	79.16602186336304	5.995137137463699	9.920281399132548	4.91855960004071
9	72.64444639628147	7.703970454764085	12.219266583295342	7.4323165656591
10	40.573742494141	25.860655566290657	19.31440330250825	14.251198637060087
11	32.88573527792068	24.633786577774433	24.629464652928522	17.851013491376367
12	29.06222595859596	22.493527569000925	29.196693588075952	19.247552884327167
13	26.62026871219187	23.52632819024277	30.750704404041418	19.102698693523944
14	21.309180744291226	25.985154885238952	31.981267941216245	20.724396429253574
15	20.1725842182823	24.783311235749846	34.979429031902775	20.06467551406508
16	25.232930836654944	26.370851823503745	29.18742236219682	19.208794977644494
17	25.375763481965723	24.999058935719034	28.022524199293713	21.60265338302153
18	27.063614551223868	23.655010017106456	29.843169894865696	19.43820553680398
19	24.59468012876548	26.05876702454993	28.01353180727561	21.333021039408983
20	25.589001677185674	28.00704892000675	27.51497686376038	18.888972539047195
21	24.49304518642135	27.577923607887094	29.943968335626742	17.985062870064816
22	23.825168380797827	23.79142948361363	30.743733557515757	21.639668578072786
23	22.250872401442688	26.187657976809387	30.561167087008712	21.000302534739212
24	23.091695909367843	25.23230346046763	29.690438647488477	21.985561982676053
25	23.2559290535124	26.603538680530285	27.556104858261776	22.584427407695536
26	21.964091775377018	25.55819053554226	27.791858887759613	24.685858801321114
27	22.75263393435972	24.66641013951452	29.456636455017826	23.12431947110793
28	20.090328229279507	26.853443528475214	28.83420956874161	24.222018673503673
29	22.215181667231302	27.2396284259968	27.352486431247236	23.192703475524663
30	24.506638337146384	25.317696330406974	28.521079142808947	21.654586189637698
31	25.859121980055015	25.49754417076901	26.88327875160504	21.760055097570937
32	25.68059860053285	27.372632177706397	25.505978895065056	21.440790326695694
33	25.29044032049164	26.82646635242091	25.081942300909137	22.801151026178317
34	21.66211470388541	27.050648776686142	28.7797672573762	22.507469262052247
35	21.201062914678232	29.357929268214473	26.76721415695279	22.6737936601545
36	23.588020181994864	30.028036744726204	24.90990180865584	21.474041264623093
37	22.871695993018	29.246813974595447	25.89021195555946	21.991278076827093
38	22.33145538727932	30.734392623171374	25.327455515542894	21.60669647400641
39	22.05764053575138	27.372144218449602	25.846853290169854	24.723361955629166
40	24.037291240573673	25.873063673106333	27.30808213887878	22.781562947441213
41	21.020796823524655	26.801859264185325	26.043500870658733	26.133843041631287
42	21.67870531861648	26.235547692440676	28.28685869954676	23.79888828939609
43	22.214693707974508	25.8964160089673	27.784400081977157	24.10449020108104
44	23.01717756000853	24.92363437631139	26.97787313895825	25.081314924721827
45	22.440549135405906	24.357810763823537	26.48970475676625	26.711935344004306
46	22.52928801167756	26.026770838997148	26.16549068485779	25.278450464467504
47	20.167356083388054	26.909698259937294	29.341896321205457	23.5810493354692
48	20.916582667966033	27.309685433579684	28.065813156218066	23.70791874223622
49	23.38809630363892	25.33045297954893	28.362771218211197	22.91867949860095
50	20.80003011405699	25.919001551710437	28.297593803196275	24.9833745310363
51	20.46535977236004	25.213273049452578	26.621802298427514	27.69956487975987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	565.0
1	1078.5
2	1592.0
3	6148.5
4	10705.0
5	6983.0
6	3261.0
7	3155.5
8	3050.0
9	3081.0
10	3112.0
11	3202.5
12	3293.0
13	3248.0
14	3203.0
15	3177.0
16	3151.0
17	3041.5
18	2932.0
19	2944.0
20	2956.0
21	3908.0
22	4860.0
23	5567.5
24	6275.0
25	7606.5
26	10706.5
27	12475.0
28	14695.0
29	16915.0
30	19738.5
31	22562.0
32	25006.5
33	27451.0
34	33037.5
35	38624.0
36	41607.5
37	44591.0
38	47864.0
39	51137.0
40	55383.0
41	59629.0
42	64855.0
43	70081.0
44	79695.0
45	89309.0
46	107049.0
47	124789.0
48	131233.0
49	137677.0
50	137785.5
51	137894.0
52	123831.5
53	109769.0
54	101221.5
55	92674.0
56	84978.5
57	77283.0
58	73313.5
59	69344.0
60	62478.0
61	55612.0
62	50731.5
63	45851.0
64	39534.0
65	33217.0
66	28036.5
67	22856.0
68	19291.5
69	15727.0
70	12395.0
71	9063.0
72	7298.0
73	5533.0
74	4300.0
75	2395.0
76	1723.0
77	1374.5
78	1026.0
79	702.5
80	379.0
81	289.5
82	200.0
83	133.0
84	66.0
85	61.5
86	57.0
87	49.0
88	41.0
89	29.5
90	18.0
91	12.5
92	7.0
93	4.0
94	1.0
95	2.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1434546.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.53746087730317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.4531245565826	19.95175082915267
2	10.777222014284279	5.935546591687276
3	4.0530657501989875	3.3483341858772606
4	2.084100888558718	2.2956338673215395
5	1.2895970275091946	1.775611384626045
6	0.8494901858358717	1.4035681654864989
7	0.652865330573551	1.2584777449171736
8	0.5110009554252949	1.125733505463088
9	0.42927602032861045	1.063905445382716
>10	6.036695780690646	41.72100550870233
>50	0.7916242347990536	13.44228582317952
>100	0.06365670788190791	3.302660696247581
>500	0.003881506561565909	0.6689690718464589
>1k	0.0041402736656703035	2.2585900832174435
>5k	2.5876710410439397E-4	0.44792709689250537
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6286	0.4381874126030117	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	3552	0.2476044685914568	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	3440	0.23979712048271717	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	3021	0.21058927354020018	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	2822	0.19671728895413598	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	2625	0.18298472129858506	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2558	0.1783142541263926	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	2106	0.14680602783040766	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	1588	0.11069704282748688	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	1460	0.1017743592746416	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1445	0.10072873229579253	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7883386102641535E-4	0.0	0.0	0.1544739590086341	0.0
2	2.7883386102641535E-4	0.0	0.0	0.7413495280039818	0.0
3	3.485423262830192E-4	0.0	0.0	1.1206332874651632	0.0
4	3.485423262830192E-4	0.0	0.0	1.6101261304970353	0.0
5	3.485423262830192E-4	0.0	0.0	3.012869576855674	0.0
6	3.485423262830192E-4	0.0	0.0	3.9313483150766864	0.0
7	3.485423262830192E-4	0.0	0.0	4.741918349080476	0.0
8	3.485423262830192E-4	0.0	0.0	5.862621345010895	0.0
9	3.485423262830192E-4	0.0	0.0	6.365358796441522	0.0
10	3.485423262830192E-4	0.0	0.0	7.489477507169515	0.0
11	4.1825079153962297E-4	0.0	0.0	8.764166502851774	0.0
12	4.1825079153962297E-4	0.0	0.0	9.864793460788292	0.0
13	4.879592567962268E-4	0.0	0.0	10.306396588188877	0.0
14	4.879592567962268E-4	0.0	0.0	10.54647254253262	0.0
15	4.879592567962268E-4	0.0	0.0	10.903867843903228	0.0
16	4.879592567962268E-4	0.0	0.0	11.50398802129733	0.0
17	4.879592567962268E-4	0.0	0.0	12.164196895742625	0.0
18	5.576677220528307E-4	0.0	0.0	12.913632605716373	0.0
19	5.576677220528307E-4	0.0	0.0	13.416858016403795	0.0
20	6.273761873094345E-4	0.0	0.0	13.939392671967298	0.0
21	6.273761873094345E-4	0.0	0.0	14.53686392768165	0.0
22	6.970846525660384E-4	0.0	0.0	15.159430230888379	0.0
23	6.970846525660384E-4	0.0	0.0	15.75013976547284	0.0
24	6.970846525660384E-4	0.0	0.0	16.233010304305335	0.0
25	6.970846525660384E-4	0.0	0.0	16.699150811476244	0.0
26	8.365015830792459E-4	0.0	0.0	17.150791957873782	0.0
27	8.365015830792459E-4	0.0	0.0	17.679460958379863	0.0
28	9.759185135924536E-4	0.0	0.0	18.113535571532736	0.0
29	9.759185135924536E-4	0.0	0.0	18.618921944643112	0.0
30	0.0010456269788490574	0.0	0.0	19.283731577795344	0.0
31	0.0011153354441056614	0.0	0.0	19.77852226418672	0.0
32	0.0011153354441056614	0.0	0.0	20.269130442662696	0.0
33	0.0011153354441056614	0.0	0.0	20.766639759199077	0.0
34	0.0011850439093622652	0.0	0.0	21.27906668730037	0.0
35	0.0011850439093622652	0.0	0.0	21.831366857528444	0.0
36	0.0011850439093622652	0.0	0.0	22.332431305792912	0.0
37	0.001254752374618869	0.0	0.0	22.825409572087615	0.0
38	0.001254752374618869	0.0	0.0	23.385586798889683	0.0
39	0.0014638777703886805	0.0	0.0	24.26168279023468	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTAC	20	7.033872E-4	45.000004	45
TCGTCGT	25	3.8912916E-5	45.0	22
CGGTCTA	75	0.0	45.0	31
TCGAATT	30	2.1658798E-6	44.999996	36
CGTTTTT	3500	0.0	40.949997	1
CGCATCG	40	3.4590266E-7	39.375004	21
AATTGCG	40	3.4590266E-7	39.375004	1
CGTAAGG	205	0.0	38.414635	2
TCGTAAG	65	9.094947E-12	38.076927	1
TAGCGAT	30	1.14006325E-4	37.499996	1
TAACGCC	60	1.5643309E-10	37.499996	12
CGTACGA	30	1.14006325E-4	37.499996	15
GCGAGAC	235	0.0	37.340427	21
TCGTCCC	170	0.0	37.058823	38
ATTGGGA	820	0.0	37.042686	4
GTCGACG	25	0.0021072824	36.0	1
GGTCGAT	25	0.0021072824	36.0	15
TCGATTG	75	1.8189894E-12	36.0	1
CGGATTA	25	0.0021072824	36.0	26
TAGTCGA	25	0.0021072824	36.0	44
>>END_MODULE