FastQCFastQC Report
Sat 14 Jan 2017
SRR2936448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643498
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC34470.53566600051593No Hit
CCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC27850.43279077790451564No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT19340.3005448346381807No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.20652744841475806No Hit
TCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC13040.20264243245511254No Hit
ACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC9510.14778600710491718No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCC7180.11157765836102054No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT207.030969E-445.021
ATAGCCG207.030969E-445.01
CCGGACT207.030969E-445.033
GCACGAC207.030969E-445.03
GCACCGT207.030969E-445.09
CCCGTAT207.030969E-445.012
CCATCGG207.030969E-445.026
TGCGATA207.030969E-445.043
GCTTACG207.030969E-445.01
GTTCCGC207.030969E-445.030
TTACGCG207.030969E-445.01
ATACGTC207.030969E-445.08
CGCAAAC207.030969E-445.030
CTTACGG207.030969E-445.02
GGCTACG207.030969E-445.01
TCCGCAA253.8888862E-544.99999628
TAAACGG302.1640062E-644.9999962
TTGTACG253.8888862E-544.9999961
TCGATAG253.8888862E-544.9999961
GTTAACG253.8888862E-544.9999961