FastQCFastQC Report
Sat 14 Jan 2017
SRR2936447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456266
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174181.1960726955102983No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC119820.8227892431739806No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC100580.6906705231049821No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG90950.6245424942970584No Hit
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC39200.26918159182457047Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTT38860.2668468535281329No Hit
CGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTG33340.2289416905977342No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT31960.21946539986513453No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC28960.19886476783774393No Hit
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC27140.1863670510744603Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCT22340.15340603983063533No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT21770.14949191974543113Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCT19210.13191271374872446No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC16310.11199876945558022No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGCAGAT16140.11083140030736142No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTC15910.10925201851859483No Hit
CGTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT15700.10780997427667749No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTA15150.10403319173832254No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG14690.1008744281607893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT351.2122291E-745.00000428
TGCGACG302.1659034E-645.0000041
TATCGCG207.0339086E-445.01
CGTTTTT89650.043.6698271
TACGGCT12050.042.385897
TTAAGCG451.9288564E-840.01
AACGTTG356.2494364E-638.571431
GTAATCG356.2494364E-638.571431
CAATGCG356.2494364E-638.571431
TATCCCG356.2494364E-638.5714327
ACGGCTG13500.037.8333328
TTTAGCG1350.036.6666641
GTTTTTT110850.036.0284162
GTCGACA250.002107292936.041
CACGACC1000.036.027
CGTAGTA250.002107292936.041
CTCGCGT250.002107292936.038
TATCGCC250.002107292936.028
AGGGATC12700.035.7874036
GACCGAT6850.035.145999
Produced by FastQC (version 0.11.5)