##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936447.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1456266 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13770629816256 33.0 31.0 34.0 30.0 34.0 2 32.20691068801991 33.0 31.0 34.0 30.0 34.0 3 32.17675204941954 33.0 31.0 34.0 30.0 34.0 4 35.84743034582968 37.0 35.0 37.0 35.0 37.0 5 34.88599678904816 37.0 35.0 37.0 33.0 37.0 6 35.38487817472907 37.0 35.0 37.0 33.0 37.0 7 35.94888433843817 37.0 35.0 37.0 35.0 37.0 8 35.944741551337465 37.0 35.0 37.0 35.0 37.0 9 37.84168139611857 39.0 38.0 39.0 35.0 39.0 10 37.53977020681661 39.0 37.0 39.0 35.0 39.0 11 37.435196591831435 39.0 37.0 39.0 35.0 39.0 12 37.38404590919516 39.0 37.0 39.0 35.0 39.0 13 37.3551164416391 39.0 37.0 39.0 34.0 39.0 14 38.45247914872695 40.0 38.0 41.0 34.0 41.0 15 38.535858833482344 40.0 38.0 41.0 34.0 41.0 16 38.59925315842023 40.0 38.0 41.0 34.0 41.0 17 38.57048712254492 40.0 38.0 41.0 34.0 41.0 18 38.482430407631576 40.0 38.0 41.0 34.0 41.0 19 38.32958813843075 40.0 37.0 41.0 34.0 41.0 20 38.21681615858641 40.0 37.0 41.0 34.0 41.0 21 38.18439969071584 40.0 36.0 41.0 34.0 41.0 22 38.23270679944461 40.0 37.0 41.0 34.0 41.0 23 38.2378418503213 40.0 36.0 41.0 34.0 41.0 24 38.196510115597015 40.0 36.0 41.0 34.0 41.0 25 38.06978807443146 40.0 36.0 41.0 34.0 41.0 26 38.07589684851531 40.0 36.0 41.0 34.0 41.0 27 38.072255343460604 40.0 36.0 41.0 34.0 41.0 28 37.970494401434905 40.0 36.0 41.0 34.0 41.0 29 37.92136395411278 40.0 36.0 41.0 34.0 41.0 30 37.77337794056855 40.0 36.0 41.0 33.0 41.0 31 37.648701542163316 40.0 36.0 41.0 33.0 41.0 32 37.48499655969445 40.0 36.0 41.0 33.0 41.0 33 37.21235200162608 40.0 36.0 41.0 32.0 41.0 34 37.000426433082964 40.0 35.0 41.0 31.0 41.0 35 36.87908321693976 40.0 35.0 41.0 31.0 41.0 36 36.77686631425852 40.0 35.0 41.0 31.0 41.0 37 36.72937773730898 40.0 35.0 41.0 31.0 41.0 38 36.64919595733197 40.0 35.0 41.0 30.0 41.0 39 36.56759754055921 40.0 35.0 41.0 30.0 41.0 40 36.45934739944488 40.0 35.0 41.0 30.0 41.0 41 36.32925921500605 40.0 35.0 41.0 30.0 41.0 42 36.383699131889365 40.0 35.0 41.0 30.0 41.0 43 36.30946681444187 39.0 35.0 41.0 30.0 41.0 44 36.22359994671303 39.0 35.0 41.0 29.0 41.0 45 36.16894990338304 39.0 35.0 41.0 29.0 41.0 46 36.08421057691383 39.0 35.0 41.0 29.0 41.0 47 35.96236333197369 39.0 35.0 41.0 29.0 41.0 48 35.92426864322864 39.0 35.0 41.0 28.0 41.0 49 35.93478183243995 39.0 35.0 41.0 28.0 41.0 50 35.824319870133614 39.0 35.0 41.0 28.0 41.0 51 34.727338274738266 38.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 10.0 9 16.0 10 23.0 11 25.0 12 20.0 13 32.0 14 29.0 15 34.0 16 88.0 17 173.0 18 348.0 19 711.0 20 1283.0 21 2222.0 22 3455.0 23 5129.0 24 7804.0 25 12734.0 26 17953.0 27 20409.0 28 19771.0 29 20039.0 30 21063.0 31 25303.0 32 32417.0 33 45589.0 34 78846.0 35 95326.0 36 95298.0 37 141578.0 38 263001.0 39 545098.0 40 433.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.388157108660096 20.161151877472935 23.594315873611002 15.85637514025597 2 34.59519071378443 25.98556857057708 23.857317275827356 15.561923439811135 3 29.205584694005076 25.715494284697986 30.307718507470476 14.771202513826456 4 25.403051365615898 23.957230341160198 33.35029452036922 17.289423772854683 5 25.141560676414887 28.81396667916438 28.568956495585283 17.475516148835446 6 24.458649724706888 32.882385498253754 28.949381500357763 13.709583276681595 7 75.27683816006142 5.109918105620814 15.278252736793965 4.334990997523804 8 76.74016972174039 6.455620058423392 12.65620429234769 4.148005927488522 9 70.61052033076375 6.941314292855838 14.256255381915118 8.191909994465297 10 36.3188455955162 30.64275345300927 20.44612728718517 12.592273664289353 11 25.252186070401972 24.223733850821212 32.659417990943965 17.864662087832855 12 23.157651143403747 22.21551557201775 34.868423763241054 19.758409521337448 13 22.853929158546585 22.09610057503231 37.33857688087204 17.71139338554907 14 18.942555824279356 26.463022552198566 35.17448048639466 19.41994113712742 15 17.50669177197023 25.780180269263997 38.06577919143893 18.64734876732685 16 21.865236158778682 26.389615633407633 32.30254637545613 19.44260183235755 17 22.376749852018794 25.68768343146101 32.13664261886222 19.798924097657984 18 21.92930412438387 25.366107565513445 33.00310520193426 19.701483108168425 19 21.60532485136644 26.542403654277447 31.605833000289785 20.246438494066332 20 22.14293267850791 27.683816006141736 32.42498279847226 17.7482685168781 21 21.606766895608356 27.59125049956533 33.739989809554025 17.061992795272293 22 21.071837150630447 23.102235443250066 34.70011659957727 21.125810806542212 23 20.016741446960925 25.873569801121498 34.23550367858619 19.874185073331383 24 20.44077112285805 25.28356769985703 33.125472956176964 21.150188221107957 25 20.116585843520348 28.479893096453534 31.239691100389628 20.163829959636494 26 19.24916189762035 26.642660063477415 31.859907461960933 22.2482705769413 27 20.598640632961285 26.413100353918857 32.64424219201712 20.34401682110274 28 18.822179464465968 26.442833932811727 33.3301745697558 21.404812032966504 29 21.026241085076492 25.49527352832518 32.791742717333236 20.686742669265094 30 21.40282063853719 26.56822311308511 32.61567598227247 19.413280266105232 31 22.908177489552045 26.038168851020348 30.846493703760164 20.20715995566744 32 24.484125839647426 26.172484971838937 30.256216927402 19.087172261111636 33 22.344818872376337 26.057464776352674 29.387007593392966 22.210708757878024 34 21.542286917362624 26.731105443648346 31.628699701840183 20.097907937148847 35 21.552381227056046 27.73394421074172 29.91163702235718 20.802037539845056 36 22.120890002238603 28.888403629556684 29.36098212826503 19.62972423993968 37 21.950110762731533 28.941003909999957 29.733166880226552 19.375718447041955 38 22.883594068666028 27.289313902817202 28.302452985924276 21.524639042592494 39 22.489091965341494 27.07712739293508 28.67738448882278 21.756396152900635 40 22.812453219398105 25.55364198573612 30.568247833843543 21.06565696102223 41 20.483689106248445 28.29098530076236 29.10038413311854 22.124941459870655 42 21.666234053394092 26.45656768749665 30.871969818700705 21.00522844040855 43 21.458442345011143 26.470301442181583 30.417451207403047 21.653805005404234 44 22.221146411438568 25.574997974271184 29.89110505910321 22.312750555187034 45 20.961005750323086 24.886868195782913 29.30034760133108 24.851778452562925 46 22.707321327284987 25.995662880270498 29.234768922710547 22.062246869733965 47 19.852554409702623 26.104777561242248 32.59933281419741 21.443335214857726 48 20.53278727924706 27.08811439668302 30.191256267742293 22.18784205632762 49 21.535076696153038 24.69761705622462 31.937846519797898 21.82945972782445 50 20.59074372401745 24.97085010568124 31.563739042180483 22.874667128120823 51 20.038784123230233 24.856104585288676 29.978589076446198 25.126522215034893 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 500.0 1 897.5 2 1295.0 3 14760.0 4 28225.0 5 18397.5 6 8570.0 7 8294.5 8 8019.0 9 8017.5 10 8016.0 11 7827.5 12 7639.0 13 7406.5 14 7174.0 15 6940.5 16 6707.0 17 6412.0 18 6117.0 19 6175.0 20 6233.0 21 6400.5 22 6568.0 23 7616.5 24 8665.0 25 10216.0 26 14054.0 27 16341.0 28 19052.0 29 21763.0 30 26457.5 31 31152.0 32 34730.5 33 38309.0 34 43247.5 35 48186.0 36 52001.0 37 55816.0 38 59879.5 39 63943.0 40 68534.5 41 73126.0 42 78720.0 43 84314.0 44 90637.5 45 96961.0 46 128038.0 47 159115.0 48 147730.5 49 136346.0 50 131759.5 51 127173.0 52 109202.0 53 91231.0 54 81491.0 55 71751.0 56 65363.5 57 58976.0 58 54376.0 59 49776.0 60 43265.0 61 36754.0 62 31881.0 63 27008.0 64 22548.0 65 18088.0 66 15867.5 67 13647.0 68 10964.5 69 8282.0 70 6675.5 71 5069.0 72 4024.5 73 2980.0 74 2618.5 75 1760.0 76 1263.0 77 977.5 78 692.0 79 438.5 80 185.0 81 170.0 82 155.0 83 117.5 84 80.0 85 50.0 86 20.0 87 12.5 88 5.0 89 4.5 90 4.0 91 3.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1456266.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.518028912545283 #Duplication Level Percentage of deduplicated Percentage of total 1 73.96176832734007 21.092438475820597 2 9.659508543893743 5.509402878714799 3 3.4898380211157236 2.9856990475883407 4 1.7879149145177382 2.0395123690155117 5 1.1796832690012076 1.6821120786511186 6 0.8236178685459012 1.4092774912848558 7 0.6360937492849167 1.269809795323761 8 0.5196135385741225 1.1854683133125432 9 0.46510320281782014 1.1937443926748408 >10 7.10845646487433 47.85045143562269 >50 0.3232528722306117 5.507320002197611 >100 0.038487866417837295 1.8901201814497022 >500 0.0014803025302391965 0.2967032729568332 >1k 0.00419419050234439 2.6718069924739827 >5k 2.467170883731994E-4 0.6399137461566038 >10k+ 7.401512651195982E-4 2.7762195267561594 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17418 1.1960726955102983 No Hit GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC 11982 0.8227892431739806 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC 10058 0.6906705231049821 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG 9095 0.6245424942970584 No Hit GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC 3920 0.26918159182457047 Illumina Single End Adapter 1 (95% over 21bp) CGTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTT 3886 0.2668468535281329 No Hit CGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTG 3334 0.2289416905977342 No Hit GAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT 3196 0.21946539986513453 No Hit CGTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC 2896 0.19886476783774393 No Hit CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC 2714 0.1863670510744603 Illumina Single End Adapter 1 (95% over 21bp) CGTTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCT 2234 0.15340603983063533 No Hit CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT 2177 0.14949191974543113 Illumina Single End Adapter 1 (95% over 22bp) GAATGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCT 1921 0.13191271374872446 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC 1631 0.11199876945558022 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGCAGAT 1614 0.11083140030736142 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTC 1591 0.10925201851859483 No Hit CGTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT 1570 0.10780997427667749 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTA 1515 0.10403319173832254 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 1469 0.1008744281607893 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.12012640547812E-4 0.0 0.0 0.15903687925145543 0.0 2 4.12012640547812E-4 0.0 0.0 0.7269963042466143 0.0 3 4.12012640547812E-4 0.0 0.0 1.1580301950330503 0.0 4 4.12012640547812E-4 0.0 0.0 1.7704183164339482 0.0 5 4.12012640547812E-4 0.0 0.0 3.5551197377402204 0.0 6 4.12012640547812E-4 0.0 0.0 4.628756010234394 0.0 7 4.12012640547812E-4 0.0 0.0 5.509501698178767 0.0 8 4.12012640547812E-4 0.0 0.0 6.592751598952389 0.0 9 4.12012640547812E-4 0.0 0.0 7.100694515974417 0.0 10 4.12012640547812E-4 0.0 0.0 8.52172611322382 0.0 11 4.12012640547812E-4 0.0 0.0 9.493663932276108 0.0 12 4.12012640547812E-4 0.0 0.0 10.99895211451754 0.0 13 4.12012640547812E-4 0.0 0.0 11.402381158387273 0.0 14 4.12012640547812E-4 0.0 0.0 11.641073814811305 0.0 15 4.8068141397244736E-4 0.0 0.0 11.99876945558023 0.0 16 4.8068141397244736E-4 0.0 0.0 12.446558527082278 0.0 17 5.493501873970827E-4 0.0 0.0 12.989659856097719 0.0 18 5.493501873970827E-4 0.0 0.0 13.56565352758356 0.0 19 5.493501873970827E-4 0.0 0.0 14.086025492595446 0.0 20 5.493501873970827E-4 0.0 0.0 14.498587483330656 0.0 21 6.18018960821718E-4 0.0 0.0 14.957432227354069 0.0 22 6.18018960821718E-4 0.0 0.0 15.502456282025399 0.0 23 6.18018960821718E-4 0.0 0.0 15.982107664396477 0.0 24 6.18018960821718E-4 0.0 0.0 16.380935900446758 0.0 25 6.866877342463533E-4 0.0 0.0 16.751472601846093 0.0 26 6.866877342463533E-4 0.0 0.0 17.098593251507623 0.0 27 6.866877342463533E-4 0.0 0.0 17.50861449762612 0.0 28 6.866877342463533E-4 0.0 0.0 17.90387195745832 0.0 29 6.866877342463533E-4 0.0 0.0 18.329412346370788 0.0 30 6.866877342463533E-4 0.0 0.0 18.90128589145115 0.0 31 6.866877342463533E-4 0.0 0.0 19.33753860901786 0.0 32 7.553565076709887E-4 0.0 0.0 19.756555464454983 0.0 33 7.553565076709887E-4 0.0 0.0 20.15710041984088 0.0 34 7.553565076709887E-4 0.0 0.0 20.559705438429518 0.0 35 7.553565076709887E-4 0.0 0.0 20.989159947427186 0.0 36 7.553565076709887E-4 0.0 0.0 21.402133950802945 0.0 37 7.553565076709887E-4 0.0 0.0 21.827056320754586 0.0 38 8.24025281095624E-4 0.0 0.0 22.30629569048512 0.0 39 8.926940545202594E-4 0.0 0.0 23.00809055488489 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGGT 35 1.2122291E-7 45.000004 28 TGCGACG 30 2.1659034E-6 45.000004 1 TATCGCG 20 7.0339086E-4 45.0 1 CGTTTTT 8965 0.0 43.669827 1 TACGGCT 1205 0.0 42.38589 7 TTAAGCG 45 1.9288564E-8 40.0 1 AACGTTG 35 6.2494364E-6 38.57143 1 GTAATCG 35 6.2494364E-6 38.57143 1 CAATGCG 35 6.2494364E-6 38.57143 1 TATCCCG 35 6.2494364E-6 38.57143 27 ACGGCTG 1350 0.0 37.833332 8 TTTAGCG 135 0.0 36.666664 1 GTTTTTT 11085 0.0 36.028416 2 GTCGACA 25 0.0021072929 36.0 41 CACGACC 100 0.0 36.0 27 CGTAGTA 25 0.0021072929 36.0 41 CTCGCGT 25 0.0021072929 36.0 38 TATCGCC 25 0.0021072929 36.0 28 AGGGATC 1270 0.0 35.787403 6 GACCGAT 685 0.0 35.14599 9 >>END_MODULE