FastQCFastQC Report
Sat 14 Jan 2017
SRR2936445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129691
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA138621.2270612052322272No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT99600.8816570194858594No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCAAT48640.4305602151384759No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATG47960.42454087002552027No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTA40680.3600984694044655No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC35570.3148648612762251No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC35140.3110585106900914No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC31960.2829092203089163No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC27260.24130492320466393No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25490.22563692195476465No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG25040.22165353180648514No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC24930.2206798142146835No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT21950.19430091945496603Illumina Single End Adapter 1 (95% over 21bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG21700.19208792492814405No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTC17870.158184848777232No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG12990.11498719561366781No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC12850.11374791867864752No Hit
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC11710.10365666363633949No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA253.8907623E-545.00000416
GTCCGAT253.8907623E-545.0000049
ACTCGAC253.8907623E-545.0000046
TACGTCC253.8907623E-545.00000433
TATCCGT302.1654687E-645.00000414
TACGATG207.0332363E-445.01
ATACGAT207.0332363E-445.011
GTACGAG800.045.01
TTGACCC50000.044.0549968
CTCAACT56800.043.9304542
TGCATTA44100.043.8265315
TCAACTT55950.043.753353
GCATTAG44700.043.64093816
CATTAGA44150.043.6240117
CTTGACC56000.043.4330377
ACTTGAC56000.043.392866
CTGCATT44700.043.33892414
AACTTGA56100.043.195195
ATTAGAA44250.043.16948718
TGACCCT45800.043.133199
Produced by FastQC (version 0.11.5)