##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936445.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1129691
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05272680759606	33.0	31.0	34.0	30.0	34.0
2	32.09494720237658	33.0	31.0	34.0	30.0	34.0
3	32.15536549375006	34.0	31.0	34.0	30.0	34.0
4	35.8205553554025	37.0	35.0	37.0	35.0	37.0
5	34.901070292672955	37.0	35.0	37.0	33.0	37.0
6	35.37924087206147	37.0	35.0	37.0	33.0	37.0
7	35.916974641738314	37.0	35.0	37.0	35.0	37.0
8	35.902920356097376	37.0	35.0	37.0	35.0	37.0
9	37.760733687353444	39.0	38.0	39.0	35.0	39.0
10	37.452341392469265	39.0	37.0	39.0	35.0	39.0
11	37.373649962688916	39.0	37.0	39.0	35.0	39.0
12	37.31792056411886	39.0	37.0	39.0	35.0	39.0
13	37.23614599036374	39.0	37.0	39.0	34.0	39.0
14	38.34458626296925	40.0	38.0	41.0	34.0	41.0
15	38.474448322594405	40.0	38.0	41.0	34.0	41.0
16	38.48244431441872	40.0	38.0	41.0	34.0	41.0
17	38.49645876615818	40.0	38.0	41.0	34.0	41.0
18	38.44525184320314	40.0	38.0	41.0	34.0	41.0
19	38.40896669974356	40.0	37.0	41.0	34.0	41.0
20	38.39778665139406	40.0	37.0	41.0	34.0	41.0
21	38.30152227467511	40.0	37.0	41.0	34.0	41.0
22	38.3486325021621	40.0	37.0	41.0	34.0	41.0
23	38.32392751646247	40.0	37.0	41.0	34.0	41.0
24	38.27946845641861	40.0	37.0	41.0	34.0	41.0
25	38.134578393560716	40.0	37.0	41.0	34.0	41.0
26	38.17871081561241	40.0	37.0	41.0	34.0	41.0
27	38.18599864918814	40.0	37.0	41.0	34.0	41.0
28	38.05275247833257	40.0	37.0	41.0	34.0	41.0
29	37.985115398812596	40.0	37.0	41.0	34.0	41.0
30	37.87928026336405	40.0	36.0	41.0	33.0	41.0
31	37.86132314057561	40.0	36.0	41.0	33.0	41.0
32	37.797622535719945	40.0	36.0	41.0	33.0	41.0
33	37.6837259038091	40.0	36.0	41.0	33.0	41.0
34	37.60044206778668	40.0	36.0	41.0	33.0	41.0
35	37.51168062771147	40.0	36.0	41.0	33.0	41.0
36	37.3596390517407	40.0	36.0	41.0	33.0	41.0
37	37.32297061762907	40.0	35.0	41.0	32.0	41.0
38	37.24349136179716	40.0	35.0	41.0	32.0	41.0
39	37.17526031454619	40.0	35.0	41.0	32.0	41.0
40	37.079767830318204	39.0	35.0	41.0	32.0	41.0
41	36.96845066482781	39.0	35.0	41.0	32.0	41.0
42	36.98473210816055	39.0	35.0	41.0	32.0	41.0
43	36.86374327139014	39.0	35.0	41.0	31.0	41.0
44	36.7211352484883	39.0	35.0	41.0	31.0	41.0
45	36.66266173670499	39.0	35.0	41.0	31.0	41.0
46	36.540374314746245	39.0	35.0	41.0	31.0	41.0
47	36.458523613979395	39.0	35.0	41.0	31.0	41.0
48	36.410710539430696	39.0	35.0	41.0	31.0	41.0
49	36.37985962533117	39.0	35.0	40.0	31.0	41.0
50	36.258223708961125	38.0	35.0	40.0	31.0	41.0
51	35.06682712352316	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	7.0
9	20.0
10	22.0
11	29.0
12	19.0
13	24.0
14	35.0
15	34.0
16	67.0
17	127.0
18	253.0
19	416.0
20	769.0
21	1180.0
22	1824.0
23	2647.0
24	3808.0
25	5403.0
26	7315.0
27	8740.0
28	10246.0
29	12489.0
30	15014.0
31	19457.0
32	25900.0
33	37205.0
34	65814.0
35	83619.0
36	84265.0
37	131088.0
38	226979.0
39	384595.0
40	277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89856075687953	20.443820478343195	23.299203056411	11.358415708366271
2	30.241986525518925	23.981425009139667	23.81607005809553	21.960518407245875
3	30.321388769141294	23.48553719556941	30.120714425449087	16.072359609840213
4	27.60790340013331	23.77659023573703	25.223269017811067	23.392237346318595
5	24.098005560812645	33.69160239392896	23.6161038726519	18.59428817260649
6	24.75756644958666	37.56947696316957	23.500497038570725	14.172459548673045
7	74.34625928683153	6.565069563269955	9.30369455010264	9.784976599795874
8	74.86179849179997	6.148583993322068	13.452085570301966	5.537531944575995
9	68.24742341047242	7.598892086420092	16.053150817347397	8.10053368576009
10	43.076469583275426	24.011964333609807	18.370510166054256	14.541055917060506
11	28.232498975383535	28.68793324900349	24.18749905947733	18.89206871613565
12	25.63656787564033	21.16295517977925	28.814870615061995	24.385606329518424
13	24.17439813187854	22.50243650697403	29.40202232291839	23.921143038229037
14	20.258371536995515	25.438283566037086	29.34528114325068	24.958063753716726
15	19.379724190066135	23.255474284561	36.20149226646932	21.16330925890354
16	25.075529503200432	23.58228931628206	29.913312578395328	21.428868602122172
17	22.284500805972606	23.56803763152933	26.356587774887114	27.79087378761095
18	22.466231916515227	27.189204835658597	29.989085510993714	20.355477736832462
19	22.908299703193176	25.567522446403483	29.8605547888759	21.663623061527446
20	23.868827847614966	26.01144914848397	30.952711847753058	19.16701115614801
21	23.875201271852216	29.69546539717498	27.425021532436745	19.00431179853606
22	26.54221375579694	23.891754470912844	26.83211603881061	22.733915734479606
23	21.914753680431197	28.303934438709344	27.532927145564585	22.248384735294874
24	21.838892228051744	28.65137457942039	27.32154190836255	22.188191284165313
25	21.109046633105866	28.803451563303597	26.332510394435293	23.754991409155245
26	20.38380406677578	27.15105281001619	29.09379644522263	23.371346677985397
27	21.460381644184118	26.42554468434289	29.227726873985894	22.8863467974871
28	21.649371376774713	25.96232066998852	29.133364787362208	23.25494316587456
29	20.616876650340668	28.339430870919568	27.671726162286852	23.371966316452905
30	21.650699173490803	29.420434437381548	26.80060299674867	22.128263392378976
31	24.474922788620958	26.288693102804217	26.335077468086403	22.901306640488418
32	24.615049602059326	28.96446904507516	24.235034181913463	22.18544717095206
33	23.6640815939934	26.042342552078402	25.01940796200023	25.274167891927966
34	21.644414269034627	25.659228939594986	29.09680611777911	23.599550673591274
35	26.15060224433053	26.681986490111015	25.344098518975542	21.823312746582914
36	23.30690427736434	28.082280906902863	27.1422008319089	21.468613983823897
37	22.24121463302797	29.23100210588559	26.91399683630302	21.613786424783417
38	23.140221529604112	28.038552135052857	25.536717562590123	23.284508772752904
39	23.19979534226616	26.2675368751278	26.725272663055648	23.80739511955039
40	24.95204440860377	23.148984987930326	29.635980104294006	22.2629904991719
41	21.698411335489084	26.956397811436933	26.81441208259604	24.530778770477944
42	22.37603025960196	24.426236909030877	27.304103511491196	25.893629319875966
43	23.021250943842166	26.491314881680033	27.25435539452824	23.23307877994956
44	22.699481539642257	25.088895990142433	27.78901487220842	24.422607598006888
45	22.964155685050162	24.834401619557912	26.602938325612936	25.59850436977899
46	22.988941223750565	25.69897432129671	27.306847624704456	24.005236830248272
47	21.714964534549715	24.1875875792584	30.25128110253158	23.846166783660312
48	20.80613194227448	25.099075764965818	28.930211889799956	25.164580402959746
49	22.289280874150542	25.06720864377958	28.70687648215308	23.936633999916793
50	21.413200600872273	23.683555945829436	30.78815357473858	24.11508987855971
51	21.385847988520755	24.277966275733807	27.31437180609565	27.02181392964979
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	367.0
1	813.0
2	1259.0
3	2911.0
4	4563.0
5	3134.0
6	1705.0
7	1750.5
8	1796.0
9	1863.5
10	1931.0
11	1990.5
12	2050.0
13	2024.0
14	1998.0
15	2131.0
16	2264.0
17	2174.0
18	2084.0
19	2330.0
20	2576.0
21	2685.0
22	2794.0
23	3258.0
24	3722.0
25	4634.0
26	6768.0
27	7990.0
28	9946.5
29	11903.0
30	20427.5
31	28952.0
32	26069.0
33	23186.0
34	25761.0
35	28336.0
36	32247.5
37	36159.0
38	41124.5
39	46090.0
40	49992.0
41	53894.0
42	59278.0
43	64662.0
44	67901.5
45	71141.0
46	87360.0
47	103579.0
48	108814.5
49	114050.0
50	109932.5
51	105815.0
52	90465.5
53	75116.0
54	68306.0
55	61496.0
56	55148.0
57	48800.0
58	48364.5
59	47929.0
60	44645.0
61	41361.0
62	38563.0
63	35765.0
64	33929.5
65	32094.0
66	27467.5
67	22841.0
68	18220.0
69	13599.0
70	11064.5
71	8530.0
72	6566.0
73	4602.0
74	4067.5
75	2767.5
76	2002.0
77	1440.0
78	878.0
79	603.5
80	329.0
81	286.5
82	244.0
83	170.5
84	97.0
85	60.0
86	23.0
87	23.0
88	23.0
89	18.5
90	14.0
91	7.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1129691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.034927127139163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8417079973426	19.422353403973684
2	11.749449144560215	6.352910028144261
3	4.393116581681598	3.5630275994036613
4	2.1295738384575547	2.302914941382481
5	1.2224044938275889	1.6523808205258153
6	0.7788398428923508	1.2633527037784331
7	0.5829036333063121	1.1031130074006559
8	0.4673617237805182	1.0108072115536362
9	0.3675387361647519	0.8942744653753406
>10	5.606321557509792	39.26366021562009
>50	0.7924716634875153	13.089844482749982
>100	0.057473017899563286	3.069734284787457
>500	0.00426942418682031	0.7814175808526549
>1k	0.005911510412520429	4.115116695529922
>5k	3.2841724514002385E-4	0.8843221344497718
>10k+	3.2841724514002385E-4	1.2307704244721627
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	13862	1.2270612052322272	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	9960	0.8816570194858594	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCAAT	4864	0.4305602151384759	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATG	4796	0.42454087002552027	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTA	4068	0.3600984694044655	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	3557	0.3148648612762251	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	3514	0.3110585106900914	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	3196	0.2829092203089163	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	2726	0.24130492320466393	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2549	0.22563692195476465	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	2504	0.22165353180648514	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	2493	0.2206798142146835	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	2195	0.19430091945496603	Illumina Single End Adapter 1 (95% over 21bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG	2170	0.19208792492814405	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTC	1787	0.158184848777232	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1299	0.11498719561366781	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	1285	0.11374791867864752	No Hit
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1171	0.10365666363633949	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.851978107287745E-5	0.0	0.0	0.20545441187014857	0.0
2	8.851978107287745E-5	0.0	0.0	1.0738334641950764	0.0
3	8.851978107287745E-5	0.0	0.0	1.5683049612681699	0.0
4	8.851978107287745E-5	0.0	0.0	2.1070363488777017	0.0
5	8.851978107287745E-5	0.0	0.0	3.6763150277376733	0.0
6	8.851978107287745E-5	0.0	0.0	4.4820220750630035	0.0
7	8.851978107287745E-5	0.0	0.0	5.264713979309386	0.0
8	8.851978107287745E-5	0.0	0.0	6.542762578439591	0.0
9	8.851978107287745E-5	0.0	0.0	7.027496899594668	0.0
10	8.851978107287745E-5	0.0	0.0	8.246502804749264	0.0
11	8.851978107287745E-5	0.0	0.0	9.645646464387164	0.0
12	8.851978107287745E-5	0.0	0.0	10.978754367344699	0.0
13	8.851978107287745E-5	0.0	0.0	11.927066781978434	0.0
14	8.851978107287745E-5	0.0	0.0	12.168283185402025	0.0
15	1.770395621457549E-4	0.0	0.0	12.955843677607417	0.0
16	1.770395621457549E-4	0.0	0.0	13.696223126500964	0.0
17	1.770395621457549E-4	0.0	0.0	14.524591237780951	0.0
18	1.770395621457549E-4	0.0	0.0	15.47237253372825	0.0
19	1.770395621457549E-4	0.0	0.0	16.082008265977155	0.0
20	1.770395621457549E-4	0.0	0.0	16.721209605104406	0.0
21	1.770395621457549E-4	0.0	0.0	17.406087151265258	0.0
22	1.770395621457549E-4	0.0	0.0	18.123185897736636	0.0
23	1.770395621457549E-4	0.0	0.0	18.87666627422897	0.0
24	1.770395621457549E-4	0.0	0.0	19.494445826336584	0.0
25	1.770395621457549E-4	0.0	0.0	20.472766446754022	0.0
26	2.6555934321863234E-4	0.0	0.0	20.979365153834102	0.0
27	2.6555934321863234E-4	0.0	0.0	21.49587807639434	0.0
28	2.6555934321863234E-4	0.0	0.0	21.97052114250711	0.0
29	2.6555934321863234E-4	0.0	0.0	22.487211104629495	0.0
30	2.6555934321863234E-4	0.0	0.0	23.103043221553506	0.0
31	2.6555934321863234E-4	0.0	0.0	23.62743440462923	0.0
32	2.6555934321863234E-4	0.0	0.0	24.323731002548485	0.0
33	2.6555934321863234E-4	0.0	0.0	24.818999177651232	0.0
34	2.6555934321863234E-4	0.0	0.0	25.623112868917254	0.0
35	2.6555934321863234E-4	0.0	0.0	26.16724396317223	0.0
36	3.540791242915098E-4	0.0	0.0	26.652420883232672	0.0
37	3.540791242915098E-4	0.0	0.0	27.16707489039038	0.0
38	4.4259890536438724E-4	0.0	0.0	27.670221326008615	0.0
39	4.4259890536438724E-4	0.0	0.0	28.307298190390117	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	3.8907623E-5	45.000004	16
GTCCGAT	25	3.8907623E-5	45.000004	9
ACTCGAC	25	3.8907623E-5	45.000004	6
TACGTCC	25	3.8907623E-5	45.000004	33
TATCCGT	30	2.1654687E-6	45.000004	14
TACGATG	20	7.0332363E-4	45.0	1
ATACGAT	20	7.0332363E-4	45.0	11
GTACGAG	80	0.0	45.0	1
TTGACCC	5000	0.0	44.054996	8
CTCAACT	5680	0.0	43.930454	2
TGCATTA	4410	0.0	43.82653	15
TCAACTT	5595	0.0	43.75335	3
GCATTAG	4470	0.0	43.640938	16
CATTAGA	4415	0.0	43.62401	17
CTTGACC	5600	0.0	43.433037	7
ACTTGAC	5600	0.0	43.39286	6
CTGCATT	4470	0.0	43.338924	14
AACTTGA	5610	0.0	43.19519	5
ATTAGAA	4425	0.0	43.169487	18
TGACCCT	4580	0.0	43.13319	9
>>END_MODULE