##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936444.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658670
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.108225666874155	33.0	31.0	34.0	30.0	34.0
2	32.13012282326506	33.0	31.0	34.0	30.0	34.0
3	32.18293986366466	34.0	31.0	34.0	30.0	34.0
4	35.83695780891797	37.0	35.0	37.0	35.0	37.0
5	34.88062155555893	37.0	35.0	37.0	33.0	37.0
6	35.38422275190915	37.0	35.0	37.0	33.0	37.0
7	35.95234032216436	37.0	35.0	37.0	35.0	37.0
8	35.944186011204394	37.0	35.0	37.0	35.0	37.0
9	37.80259462249685	39.0	38.0	39.0	35.0	39.0
10	37.468021922965974	39.0	37.0	39.0	35.0	39.0
11	37.389357341309	39.0	37.0	39.0	35.0	39.0
12	37.320242306466064	39.0	37.0	39.0	35.0	39.0
13	37.261161127727085	39.0	37.0	39.0	34.0	39.0
14	38.39548180424188	40.0	38.0	41.0	34.0	41.0
15	38.531256926837415	40.0	38.0	41.0	34.0	41.0
16	38.51713908330424	40.0	38.0	41.0	34.0	41.0
17	38.532219472573516	40.0	38.0	41.0	35.0	41.0
18	38.50169280519835	40.0	38.0	41.0	35.0	41.0
19	38.42962333186573	40.0	37.0	41.0	34.0	41.0
20	38.43626246830735	40.0	37.0	41.0	34.0	41.0
21	38.343381359406074	40.0	37.0	41.0	34.0	41.0
22	38.38198946361607	40.0	37.0	41.0	34.0	41.0
23	38.39034873305297	40.0	37.0	41.0	34.0	41.0
24	38.36022742799884	40.0	37.0	41.0	34.0	41.0
25	38.21315529779707	40.0	37.0	41.0	34.0	41.0
26	38.21776306800067	40.0	37.0	41.0	34.0	41.0
27	38.22251810466546	40.0	37.0	41.0	34.0	41.0
28	38.13682876098805	40.0	37.0	41.0	34.0	41.0
29	38.11546905126998	40.0	37.0	41.0	34.0	41.0
30	38.019205368393884	40.0	37.0	41.0	34.0	41.0
31	37.97163374679278	40.0	37.0	41.0	34.0	41.0
32	37.902665978411044	40.0	36.0	41.0	34.0	41.0
33	37.76784581049691	40.0	36.0	41.0	33.0	41.0
34	37.65122595533423	40.0	36.0	41.0	33.0	41.0
35	37.59415488788012	40.0	36.0	41.0	33.0	41.0
36	37.534326749358556	40.0	36.0	41.0	33.0	41.0
37	37.42874732415322	40.0	36.0	41.0	33.0	41.0
38	37.371618564683374	40.0	35.0	41.0	33.0	41.0
39	37.31816994853265	40.0	35.0	41.0	33.0	41.0
40	37.20264472345788	40.0	35.0	41.0	32.0	41.0
41	37.13702461019934	39.0	35.0	41.0	32.0	41.0
42	37.12042297356795	39.0	35.0	41.0	32.0	41.0
43	36.97308212002976	39.0	35.0	41.0	32.0	41.0
44	36.79153141937541	39.0	35.0	41.0	31.0	41.0
45	36.7981432280201	39.0	35.0	41.0	31.0	41.0
46	36.69041553433434	39.0	35.0	41.0	31.0	41.0
47	36.584172650948126	39.0	35.0	41.0	31.0	41.0
48	36.53702005556652	39.0	35.0	41.0	31.0	41.0
49	36.499965081148375	39.0	35.0	41.0	31.0	41.0
50	36.356501738351525	39.0	35.0	40.0	31.0	41.0
51	35.13489304203926	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	12.0
10	17.0
11	14.0
12	14.0
13	7.0
14	15.0
15	21.0
16	30.0
17	54.0
18	101.0
19	171.0
20	331.0
21	548.0
22	915.0
23	1413.0
24	2106.0
25	2997.0
26	4032.0
27	4904.0
28	6012.0
29	6902.0
30	8576.0
31	10907.0
32	14415.0
33	20537.0
34	37469.0
35	52711.0
36	46989.0
37	72018.0
38	130022.0
39	234233.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51031624333885	22.634855086765754	24.226243794312783	11.628584875582613
2	31.406774257215297	24.338591403889655	26.501586530432537	17.753047808462508
3	31.505761610518167	24.700381070945998	27.417826832860158	16.37603048567568
4	28.623741782683286	26.51585771327068	26.399107291967145	18.461293212078886
5	24.497244447143487	31.832480604855242	24.471586682253633	19.19868826574764
6	26.056295261663653	34.651039215388586	24.31718462963244	14.975480893315318
7	79.9647775061867	6.600118420453338	8.534926442679946	4.900177630680007
8	80.80571454597903	5.41697663473363	8.608711494375028	5.168597324912323
9	74.087934777658	8.260433904686717	10.88921614769156	6.762415169963715
10	39.96993942338348	28.229310580412044	17.543838340899086	14.256911655305387
11	28.1444425888533	26.34308530827273	25.80199492917546	19.71047717369851
12	25.31191643766985	23.098364886817375	31.12757526530736	20.462143410205414
13	23.427664839752836	24.820016093035964	32.036376334127866	19.715942733083335
14	19.025460397467626	29.29190641747764	30.186588124554024	21.496045060500705
15	18.19712450847921	26.621828836898597	34.9054913689708	20.27555528565139
16	21.104953922297966	26.507203910911382	31.379142818103144	21.008699348687507
17	21.03223161826104	26.15892632122307	28.5259689981326	24.28287306238329
18	21.981113455903564	24.87345711813199	31.81335114700837	21.33207827895608
19	22.43703220125404	27.37577238981584	28.40770036588884	21.77949504304128
20	23.798108309168477	26.975420164877704	29.258202134604584	19.96826939134923
21	23.68682344724976	26.594045576692427	29.965081148374757	19.75404982768306
22	22.122914357720862	25.16829368272428	29.17667420711434	23.532117752440524
23	20.43709292969165	25.304932667344797	30.288915541925395	23.969058861038153
24	21.736074210150758	24.82669622117297	30.35343950688509	23.083790061791184
25	20.54276041113152	27.54095374011265	28.197428150667253	23.718857698088573
26	19.554556910137094	29.331379901923572	28.263773968755217	22.850289219184113
27	21.666995612370382	27.986548651069583	28.70299239376319	21.64346334279685
28	19.033203273262785	27.97182200494937	30.582689358859522	22.412285362928326
29	20.0845643493707	27.650872212185163	29.98254057418738	22.28202286425676
30	22.168005222645633	27.098850714318246	29.431581823978625	21.301562239057496
31	22.039109113820274	29.522219017110235	26.102145232058543	22.336526637010945
32	22.47483565366572	30.663002717597582	25.662623164862524	21.19953846387417
33	23.371339213870375	27.981690376060854	26.635796377548697	22.011174032520078
34	21.291693867946012	27.83579030470494	27.918988264229434	22.953527563119618
35	21.87741964868599	28.193025338940593	26.64854934944661	23.281005662926805
36	24.597446369198536	27.715244356050828	27.514233227564638	20.173076047185994
37	22.682071447006848	28.041659708199855	28.195909939727027	21.08035890506627
38	21.84492993456511	28.812151760365584	26.420210424036316	22.92270788103299
39	22.12169978896868	26.21160824084898	29.090136183521338	22.576555786660997
40	22.59401521247362	25.36004372447508	29.158759318019644	22.887181745031654
41	22.200646757860536	25.367786600270243	27.11479192919064	25.31677471267858
42	23.502512639106076	25.72137792824935	27.63143911215024	23.144670320494328
43	24.321739262453125	25.34835350023532	27.75426237721469	22.575644860096862
44	21.04665462219321	26.996371475852854	27.96058724399168	23.99638665796226
45	21.4860248682952	26.248500766696527	27.500417508008564	24.765056856999713
46	23.34826240757891	25.73580093218152	28.106031852065527	22.809904808174046
47	20.94888183764252	25.69222827819697	29.343981052727464	24.01490883143304
48	21.75945465863027	25.129275661560417	29.05263637329771	24.058633306511606
49	22.569875658523994	23.832571697511653	30.324441677926732	23.27311096603762
50	21.115733219973585	23.7809525255439	29.994230798427136	25.109083456055387
51	20.47702187741965	24.08110282842698	28.2736423398667	27.16823295428667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	379.0
1	593.0
2	807.0
3	1765.5
4	2724.0
5	1962.5
6	1201.0
7	1184.5
8	1168.0
9	1212.5
10	1257.0
11	1310.5
12	1364.0
13	1375.5
14	1387.0
15	1389.0
16	1391.0
17	1560.5
18	1730.0
19	1859.5
20	1989.0
21	2092.0
22	2195.0
23	2298.5
24	2402.0
25	3894.0
26	5723.5
27	6061.0
28	7074.5
29	8088.0
30	9424.5
31	10761.0
32	12764.0
33	14767.0
34	16846.0
35	18925.0
36	20799.0
37	22673.0
38	24636.5
39	26600.0
40	29555.5
41	32511.0
42	36596.0
43	40681.0
44	45624.5
45	50568.0
46	56089.5
47	61611.0
48	66500.5
49	71390.0
50	69172.0
51	66954.0
52	57168.0
53	47382.0
54	40940.5
55	34499.0
56	31467.5
57	28436.0
58	25810.5
59	23185.0
60	21746.0
61	20307.0
62	18103.0
63	15899.0
64	13964.5
65	12030.0
66	9859.5
67	7689.0
68	6220.0
69	4751.0
70	3780.5
71	2810.0
72	2440.5
73	2071.0
74	1716.5
75	909.0
76	456.0
77	387.0
78	318.0
79	286.5
80	255.0
81	178.5
82	102.0
83	120.5
84	139.0
85	73.0
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	658670.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.489167264186012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.89333062140769	21.200744518741168
2	11.891859395612716	7.013620615988106
3	4.374084586518941	3.8696433599866484
4	2.1138573847511304	2.4934357596624355
5	1.263043949467476	1.8623057143932247
6	0.8364200124774324	1.479919779063573
7	0.5486001731885132	1.1324433587822234
8	0.42289262388571475	0.9976601056445076
9	0.3481587350791669	0.924022005591328
>10	5.556356070526995	41.754206385466844
>50	0.6846860949343561	12.417508506504051
>100	0.06205614153480067	3.1564172948468827
>500	0.002585672563950028	0.5316103338407688
>1k	0.0020685380511600224	1.1664622614882503
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	2443	0.370898932697709	No Hit
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	2119	0.3217088982343207	No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT	1701	0.25824768093278877	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1386	0.21042403631560566	No Hit
GGGGAAGGGGAAGAAGATTGATCAAACTGAATGAAACACACACACACACAC	860	0.13056614085961105	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC	783	0.11887591661985517	No Hit
TCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	706	0.10718569238009928	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5182109402280352E-4	0.0	0.0	0.27661803330954804	0.0
2	1.5182109402280352E-4	0.0	0.0	1.2467548241152626	0.0
3	1.5182109402280352E-4	0.0	0.0	1.7234730593468657	0.0
4	1.5182109402280352E-4	0.0	0.0	2.301152322103633	0.0
5	1.5182109402280352E-4	0.0	0.0	3.8076730380919126	0.0
6	1.5182109402280352E-4	0.0	0.0	4.6429926974053775	0.0
7	1.5182109402280352E-4	0.0	0.0	5.484081558291709	0.0
8	1.5182109402280352E-4	0.0	0.0	6.765907055126239	0.0
9	1.5182109402280352E-4	0.0	0.0	7.266461202119422	0.0
10	1.5182109402280352E-4	0.0	0.0	8.3894818346061	0.0
11	1.5182109402280352E-4	0.0	0.0	10.079706074361972	0.0
12	1.5182109402280352E-4	0.0	0.0	11.381268313419467	0.0
13	1.5182109402280352E-4	0.0	0.0	11.88561798776322	0.0
14	1.5182109402280352E-4	0.0	0.0	12.101052120181578	0.0
15	1.5182109402280352E-4	0.0	0.0	12.493054184948457	0.0
16	1.5182109402280352E-4	0.0	0.0	13.29527684576495	0.0
17	1.5182109402280352E-4	0.0	0.0	14.306253510862799	0.0
18	1.5182109402280352E-4	0.0	0.0	15.459031077777947	0.0
19	3.0364218804560704E-4	0.0	0.0	16.158015394658936	0.0
20	3.0364218804560704E-4	0.0	0.0	16.800522264563437	0.0
21	3.0364218804560704E-4	0.0	0.0	17.58042722455858	0.0
22	3.0364218804560704E-4	0.0	0.0	18.390089118982193	0.0
23	3.0364218804560704E-4	0.0	0.0	19.176066922738244	0.0
24	3.0364218804560704E-4	0.0	0.0	19.829201269224345	0.0
25	4.554632820684106E-4	0.0	0.0	20.392153885860903	0.0
26	4.554632820684106E-4	0.0	0.0	20.917151228991756	0.0
27	4.554632820684106E-4	0.0	0.0	21.433191127575267	0.0
28	6.072843760912141E-4	0.0	0.0	21.981720740279656	0.0
29	6.072843760912141E-4	0.0	0.0	22.569723837429972	0.0
30	7.591054701140176E-4	0.0	0.0	23.219214477659527	0.0
31	7.591054701140176E-4	0.0	0.0	23.82315878968224	0.0
32	7.591054701140176E-4	0.0	0.0	24.36698194847192	0.0
33	7.591054701140176E-4	0.0	0.0	24.898963061927823	0.0
34	7.591054701140176E-4	0.0	0.0	25.456146476991513	0.0
35	7.591054701140176E-4	0.0	0.0	26.03959494132115	0.0
36	7.591054701140176E-4	0.0	0.0	26.594501039974492	0.0
37	7.591054701140176E-4	0.0	0.0	27.15092534956807	0.0
38	7.591054701140176E-4	0.0	0.0	27.722531768563925	0.0
39	9.109265641368212E-4	0.0	0.0	28.293834545371734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	70	0.0	45.000004	6
ATAACGG	35	1.2108649E-7	45.000004	2
CGAACTC	20	7.031091E-4	45.0	13
TTTACGG	45	3.8380676E-10	45.0	2
TTGCTCG	25	3.888985E-5	45.0	1
GCGATAT	40	6.8066583E-9	45.0	9
TCCCGCT	20	7.031091E-4	45.0	38
TCATCCG	20	7.031091E-4	45.0	25
CGTCCGG	25	3.888985E-5	45.0	2
TTACCGG	40	6.8066583E-9	45.0	2
TATCGTA	20	7.031091E-4	45.0	28
TTCTCGT	25	3.888985E-5	45.0	19
TACGGGT	70	0.0	41.785717	4
TTGCGAG	55	6.002665E-11	40.909092	1
GGCGATA	205	0.0	40.609756	8
CGTTCAT	50	1.0804797E-9	40.5	17
CCTCATA	50	1.0804797E-9	40.5	18
TACACGG	50	1.0804797E-9	40.5	2
TAGTAGT	95	0.0	40.26316	21
GACACGT	45	1.9261279E-8	40.0	9
>>END_MODULE