##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936443.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414194
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8519751613978	33.0	31.0	34.0	30.0	34.0
2	31.895901437490643	33.0	31.0	34.0	30.0	34.0
3	31.93407919960212	33.0	31.0	34.0	30.0	34.0
4	35.61975789122971	37.0	35.0	37.0	33.0	37.0
5	34.7302664934789	37.0	35.0	37.0	33.0	37.0
6	35.209426017759796	37.0	35.0	37.0	33.0	37.0
7	35.769091295383326	37.0	35.0	37.0	35.0	37.0
8	35.71483411155159	37.0	35.0	37.0	35.0	37.0
9	37.595322481735614	39.0	37.0	39.0	35.0	39.0
10	37.24183595126921	39.0	37.0	39.0	34.0	39.0
11	37.19079706610912	39.0	37.0	39.0	34.0	39.0
12	37.0359227801465	39.0	37.0	39.0	33.0	39.0
13	36.936148278343	39.0	37.0	39.0	33.0	39.0
14	37.96322979087095	40.0	37.0	41.0	33.0	41.0
15	38.05966286329594	40.0	37.0	41.0	33.0	41.0
16	38.15466665379025	40.0	37.0	41.0	34.0	41.0
17	38.1185845280231	40.0	37.0	41.0	34.0	41.0
18	38.142467056500095	40.0	37.0	41.0	34.0	41.0
19	38.06907391222471	40.0	37.0	41.0	34.0	41.0
20	37.98890857907164	40.0	36.0	41.0	34.0	41.0
21	37.96343259438814	40.0	36.0	41.0	34.0	41.0
22	38.03814396152528	40.0	36.0	41.0	34.0	41.0
23	38.0566304678484	40.0	36.0	41.0	34.0	41.0
24	38.0010912760687	40.0	36.0	41.0	34.0	41.0
25	37.892316161025995	40.0	36.0	41.0	34.0	41.0
26	37.946242581978495	40.0	36.0	41.0	34.0	41.0
27	37.926049146052335	40.0	36.0	41.0	34.0	41.0
28	37.755727992196896	40.0	36.0	41.0	33.0	41.0
29	37.729365949289466	40.0	36.0	41.0	33.0	41.0
30	37.61144536135241	40.0	36.0	41.0	33.0	41.0
31	37.46661709247358	39.0	36.0	41.0	33.0	41.0
32	37.38164000444236	39.0	35.0	41.0	33.0	41.0
33	37.261080556454225	39.0	35.0	41.0	32.0	41.0
34	37.18871108707514	39.0	35.0	41.0	32.0	41.0
35	37.152537216859734	39.0	35.0	41.0	32.0	41.0
36	37.03043984219955	39.0	35.0	41.0	31.0	41.0
37	36.922852576328964	39.0	35.0	41.0	31.0	41.0
38	36.76863257314206	39.0	35.0	41.0	31.0	41.0
39	36.82980197685143	39.0	35.0	41.0	31.0	41.0
40	36.73502513315017	39.0	35.0	41.0	31.0	41.0
41	36.65649912842774	39.0	35.0	41.0	31.0	41.0
42	36.68690517004109	39.0	35.0	41.0	31.0	41.0
43	36.55088195386703	39.0	35.0	41.0	31.0	41.0
44	36.415923456158225	39.0	35.0	41.0	30.0	41.0
45	36.37858829437413	39.0	35.0	40.0	30.0	41.0
46	36.223851625083896	39.0	35.0	40.0	30.0	41.0
47	36.01347919091054	38.0	35.0	40.0	29.0	41.0
48	36.03040121295818	38.0	35.0	40.0	29.0	41.0
49	36.04431739716172	38.0	35.0	40.0	29.0	41.0
50	35.930556695654694	38.0	35.0	40.0	29.0	41.0
51	34.576251708136766	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	0.0
10	11.0
11	4.0
12	10.0
13	4.0
14	6.0
15	13.0
16	18.0
17	44.0
18	90.0
19	148.0
20	325.0
21	554.0
22	804.0
23	1173.0
24	1736.0
25	2478.0
26	3162.0
27	3743.0
28	4252.0
29	5315.0
30	6554.0
31	8311.0
32	11322.0
33	15468.0
34	26836.0
35	34955.0
36	31635.0
37	48023.0
38	86106.0
39	121033.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.605880336267546	21.966759537801128	23.88953002699218	11.537830098939144
2	30.680309227077167	27.97167510876546	24.005176318343576	17.3428393458138
3	29.44707069634036	27.131247676209696	27.58586556058272	15.835816066867217
4	28.119673389764216	23.07397016856835	30.673549109837417	18.132807331830012
5	25.798055983428053	29.163145772270965	26.27271278676176	18.76608545753922
6	24.201944016571943	34.10527434004356	26.036108683370596	15.656672960013907
7	72.50370599284393	6.673684312182214	15.003597348102579	5.819012346871273
8	70.39672230886976	8.849959197863804	12.34107688667629	8.412241606590149
9	65.62432097036654	7.155825531031352	15.188051975644262	12.031801522957842
10	37.564522904725806	23.166921780614878	24.022559476960073	15.245995837699242
11	29.937662061739186	23.87818268733975	26.947758779702262	19.2363964712188
12	24.47572876478172	20.84820156738147	33.97345205386848	20.702617613968332
13	22.159422879133935	25.07593060256788	35.13281216048518	17.631834357813005
14	18.31460619902751	29.25295875845618	31.271578052796517	21.160856989719793
15	14.907265677436177	25.634847438639863	40.62420025398726	18.833686629936697
16	17.29793285272119	27.055437790021102	31.50407779929212	24.142551557965593
17	17.21415568550003	27.110725891731896	30.967131344249317	24.707987078518762
18	20.035297469301824	24.524256749252764	33.21800895232669	22.22243682911872
19	20.24413680545831	26.760406959057832	29.78193793246643	23.213518303017427
20	20.71227492431083	26.040454473024717	33.12578163855584	20.121488964108604
21	20.475912253678228	28.27105172938285	32.27014394221065	18.982892074728266
22	17.43023800441339	26.31399778847593	30.12911823927918	26.126645967831497
23	16.994451875208235	27.938115955325284	31.51421797515174	23.55321419431474
24	21.158201229375607	24.72995745954794	29.854609192793713	24.257232118282737
25	16.835106254557044	29.470731106679477	29.363052096360644	24.331110542402836
26	19.273818548795973	25.635330304156987	30.904117394264524	24.186733752782512
27	22.39819987735216	26.184348397127916	29.307039696374165	22.110412029145763
28	16.59463922702888	25.370961433531146	34.45342037789055	23.580978961549416
29	22.066712699845965	24.47355586995466	30.618502440885187	22.841228989314185
30	25.934224059257254	25.389793188699016	29.450450754960233	19.225531997083493
31	24.65245754404941	26.527424347045102	27.48856815888207	21.33154995002342
32	24.256990685524173	31.53208399928536	24.038494039025192	20.172431276165277
33	24.999879283620718	27.81232948811427	24.80383588366804	22.383955344596977
34	24.343182180330956	24.915860683641	29.23388557052975	21.507071565498293
35	23.080730285808098	28.75657300685186	25.52016687832272	22.64252982901732
36	27.380164850287546	26.546497534971536	27.717446413999237	18.35589120074168
37	22.374056601495916	27.51874725370237	31.940829659531524	18.16636648527019
38	21.353278898294036	30.31502146337224	27.45549187095902	20.87620776737471
39	25.67564957483691	26.603234233233703	27.963707827732897	19.75740836419649
40	26.33814106433217	25.879418823063588	27.869066186376436	19.913373926227806
41	22.640598366948822	28.17230573113082	24.34776940274364	24.839326499176714
42	22.94552794101315	26.519215633253985	28.05134791909105	22.483908506641814
43	24.129997054520345	25.513889626600093	28.36086471556807	21.99524860331149
44	22.45566087389001	23.297054037480024	30.009850456549348	24.23743463208062
45	22.82167293587063	22.02494483261467	27.721550770894797	27.431831460619904
46	28.245942722492355	24.24540191311318	27.860374607068184	19.64828075732628
47	20.454424738166175	25.047200104298952	33.4633046350261	21.035070522508775
48	21.28471199486231	25.267869645624998	29.00742164299821	24.439996716514482
49	22.883238289304046	21.185241698334597	34.47345929685123	21.458060715510126
50	23.506859104670756	21.06742251215614	31.1380657373115	24.287652645861602
51	21.425708725862762	21.19948623108978	27.063163638295094	30.31164140475236
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	537.0
1	574.0
2	611.0
3	1291.0
4	1971.0
5	1379.5
6	788.0
7	789.0
8	790.0
9	815.0
10	840.0
11	892.5
12	945.0
13	999.0
14	1053.0
15	1008.5
16	964.0
17	1089.0
18	1214.0
19	1126.5
20	1039.0
21	1299.0
22	1559.0
23	1447.5
24	1336.0
25	1761.5
26	2411.5
27	2636.0
28	3794.0
29	4952.0
30	5381.5
31	5811.0
32	6700.0
33	7589.0
34	8445.0
35	9301.0
36	11477.0
37	13653.0
38	14570.5
39	15488.0
40	16772.0
41	18056.0
42	21212.5
43	24369.0
44	28287.0
45	32205.0
46	45253.0
47	58301.0
48	57403.0
49	56505.0
50	55123.0
51	53741.0
52	42369.5
53	30998.0
54	24968.0
55	18938.0
56	16707.0
57	14476.0
58	12149.5
59	9823.0
60	8419.5
61	7016.0
62	6054.0
63	5092.0
64	4243.5
65	3395.0
66	2811.5
67	2228.0
68	1915.0
69	1602.0
70	1142.5
71	683.0
72	677.0
73	671.0
74	509.0
75	294.0
76	241.0
77	192.5
78	144.0
79	101.0
80	58.0
81	37.5
82	17.0
83	16.5
84	16.0
85	10.5
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	414194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.30466367311782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.18641825658933	23.375350544564842
2	12.691564573611236	8.453765792195584
3	5.217954314438873	5.213466405122421
4	2.584050029096889	3.4424366853432877
5	1.5443763240886752	2.5717467029249668
6	1.0351220117234858	2.068463427665509
7	0.7280252844781457	1.69726460715494
8	0.5332146649948072	1.4206828066580994
9	0.4315666918171749	1.2935865171141991
>10	4.785895355009556	35.05567137881722
>50	0.18493680334823667	3.981054547223797
>100	0.06527181294643647	4.072839568941127
>500	0.005801938928572131	1.4574495290588756
>1k	0.005801938928572131	5.89622148721515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC	4633	1.1185579704196584	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	3960	0.9560737239071547	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG	3940	0.9512450687359064	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	3759	0.9075457394361096	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC	3694	0.8918526101295529	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	2169	0.5236676533218733	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1145	0.2764405085539626	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1111	0.2682317947628406	No Hit
ACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	944	0.22791252408291768	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCT	938	0.2264639275315432	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT	796	0.1921804758156806	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC	715	0.17262442237212514	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTA	704	0.1699686620279386	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGGAAT	695	0.16779576720087688	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	639	0.15427553272138178	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTC	603	0.14558395341313493	No Hit
ACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	486	0.11733632066133261	No Hit
AGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	457	0.11033477066302265	No Hit
AAGCAGAGGTGATGTTTTGTTTTAGATGTTTTCCTGAATAATCTGTAATAT	451	0.10888617411164815	No Hit
GCCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	438	0.1057475482503368	No Hit
GGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	432	0.10429895169896232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4143275856241278E-4	0.0	0.0	0.559158268830548	0.0
2	2.4143275856241278E-4	0.0	0.0	3.0797162682221373	0.0
3	2.4143275856241278E-4	0.0	0.0	4.007300926618927	0.0
4	2.4143275856241278E-4	0.0	0.0	5.228226386669049	0.0
5	2.4143275856241278E-4	0.0	0.0	8.751454632370338	0.0
6	2.4143275856241278E-4	0.0	0.0	10.06750459929405	0.0
7	2.4143275856241278E-4	0.0	0.0	11.54941887135014	0.0
8	2.4143275856241278E-4	0.0	0.0	13.819611100112507	0.0
9	2.4143275856241278E-4	0.0	0.0	14.552600955107993	0.0
10	2.4143275856241278E-4	0.0	0.0	17.259303611351204	0.0
11	2.4143275856241278E-4	0.0	0.0	19.808109243494595	0.0
12	4.8286551712482556E-4	0.0	0.0	22.972568409972137	0.0
13	4.8286551712482556E-4	0.0	0.0	23.88518423733806	0.0
14	4.8286551712482556E-4	0.0	0.0	24.27051092000367	0.0
15	4.8286551712482556E-4	0.0	0.0	25.047200104298952	0.0
16	4.8286551712482556E-4	0.0	0.0	26.328483753989676	0.0
17	4.8286551712482556E-4	0.0	0.0	27.869307619134993	0.0
18	4.8286551712482556E-4	0.0	0.0	29.565131315277384	0.0
19	4.8286551712482556E-4	0.0	0.0	30.7616720667127	0.0
20	4.8286551712482556E-4	0.0	0.0	32.112971216386526	0.0
21	4.8286551712482556E-4	0.0	0.0	33.29285310748104	0.0
22	7.242982756872383E-4	0.0	0.0	34.470079238231364	0.0
23	7.242982756872383E-4	0.0	0.0	35.47419808109243	0.0
24	7.242982756872383E-4	0.0	0.0	36.28613644813783	0.0
25	7.242982756872383E-4	0.0	0.0	37.043993877265244	0.0
26	7.242982756872383E-4	0.0	0.0	37.717349840895814	0.0
27	7.242982756872383E-4	0.0	0.0	38.484381714848595	0.0
28	7.242982756872383E-4	0.0	0.0	39.29318145603268	0.0
29	7.242982756872383E-4	0.0	0.0	40.05852330067553	0.0
30	7.242982756872383E-4	0.0	0.0	40.841731169452	0.0
31	7.242982756872383E-4	0.0	0.0	41.55323350893543	0.0
32	7.242982756872383E-4	0.0	0.0	42.22151938463619	0.0
33	7.242982756872383E-4	0.0	0.0	42.86348908965364	0.0
34	7.242982756872383E-4	0.0	0.0	43.50401019811972	0.0
35	7.242982756872383E-4	0.0	0.0	44.19619791691816	0.0
36	7.242982756872383E-4	0.0	0.0	44.83068320642018	0.0
37	7.242982756872383E-4	0.0	0.0	45.461788437302324	0.0
38	7.242982756872383E-4	0.0	0.0	46.06054167853711	0.0
39	7.242982756872383E-4	0.0	0.0	46.71627305079262	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTATG	20	7.028054E-4	45.000004	1
CGGCGCA	20	7.028054E-4	45.000004	22
CGAACTG	20	7.028054E-4	45.000004	11
CTCGTAG	25	3.8864688E-5	45.000004	1
GTCGACG	45	3.8380676E-10	45.000004	1
GGGCTAA	30	2.1621254E-6	45.000004	7
GTTTCGT	60	0.0	45.000004	10
TTTACGG	20	7.028054E-4	45.000004	2
CGAAAGG	50	2.1827873E-11	45.000004	2
CACGGCG	20	7.028054E-4	45.000004	2
TTGCTCG	25	3.8864688E-5	45.000004	1
GTCTAGA	20	7.028054E-4	45.000004	39
CGATTGA	20	7.028054E-4	45.000004	10
CACGAGG	50	2.1827873E-11	45.000004	2
TCGATTG	35	1.2094097E-7	45.000004	1
CGTTCTA	25	3.8864688E-5	45.000004	41
GCGTTAG	35	1.2094097E-7	45.000004	1
AGACGAC	35	1.2094097E-7	45.000004	38
ACGATAG	25	3.8864688E-5	45.000004	1
CGCTCAC	25	3.8864688E-5	45.000004	19
>>END_MODULE