SRR2936442.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936442.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	512850
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	4913	0.957979916154821	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	4793	0.9345812615774594	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	4230	0.8248025738520036	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	2367	0.46153846153846156	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	1443	0.28136882129277563	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	1381	0.2692795164278054	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	959	0.18699424783074972	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	930	0.18133957297455397	No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	914	0.17821975236423906	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	834	0.1626206493126645	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	818	0.1595008287023496	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT	651	0.12693770108218777	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC	610	0.11894316076825583	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA	561	0.10938871014916643	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	60	0.0	45.000004	4
CGGTCTA	30	2.1631422E-6	45.000004	31
CTCGTAG	25	3.8877737E-5	45.0	1
TGCGATT	20	7.029629E-4	45.0	38
ATGACGG	20	7.029629E-4	45.0	2
TATCGAT	20	7.029629E-4	45.0	16
CTTACGG	25	3.8877737E-5	45.0	2
TTACGGG	110	0.0	40.909092	3
TCGATCA	50	1.0786607E-9	40.5	17
GCCTCGA	50	1.0786607E-9	40.5	14
CAACGAG	85	0.0	39.705883	14
CTACGGG	85	0.0	39.705883	3
TTCGCAC	40	3.453224E-7	39.375	43
TCAACGG	40	3.453224E-7	39.375	2
CACGACG	35	6.2415256E-6	38.571426	26
GCCGATA	35	6.2415256E-6	38.571426	9
TTAAGCG	35	6.2415256E-6	38.571426	1
CGTTTTT	605	0.0	37.561985	1
CAACGGG	120	0.0	37.500004	3
TAGACGG	60	1.546141E-10	37.500004	2