##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936442.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	512850
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.977901920639564	33.0	31.0	34.0	30.0	34.0
2	32.022335965681975	33.0	31.0	34.0	30.0	34.0
3	32.057611387345226	33.0	31.0	34.0	30.0	34.0
4	35.724229306814856	37.0	35.0	37.0	33.0	37.0
5	34.79309934678756	37.0	35.0	37.0	33.0	37.0
6	35.249685093107146	37.0	35.0	37.0	33.0	37.0
7	35.77925709271717	37.0	35.0	37.0	35.0	37.0
8	35.75217899970752	37.0	35.0	37.0	35.0	37.0
9	37.58574046992298	39.0	37.0	39.0	35.0	39.0
10	37.285065808716	39.0	37.0	39.0	34.0	39.0
11	37.18539923954373	39.0	37.0	39.0	34.0	39.0
12	37.031884566637416	39.0	37.0	39.0	33.0	39.0
13	36.87976406356635	39.0	35.0	39.0	33.0	39.0
14	37.79082772740568	40.0	37.0	41.0	33.0	41.0
15	37.92442234571512	40.0	37.0	41.0	33.0	41.0
16	38.04114263429853	40.0	37.0	41.0	33.0	41.0
17	38.021569659744564	40.0	36.0	41.0	33.0	41.0
18	38.0393487374476	40.0	37.0	41.0	33.0	41.0
19	37.980721458516136	40.0	36.0	41.0	33.0	41.0
20	37.8931051964512	40.0	36.0	41.0	33.0	41.0
21	37.831227454421374	40.0	36.0	41.0	33.0	41.0
22	37.896638393292385	40.0	36.0	41.0	33.0	41.0
23	37.90795164278054	40.0	36.0	41.0	34.0	41.0
24	37.87552695719996	40.0	36.0	41.0	33.0	41.0
25	37.71552500731208	40.0	36.0	41.0	33.0	41.0
26	37.71836209417958	40.0	36.0	41.0	33.0	41.0
27	37.698085210100416	40.0	36.0	41.0	33.0	41.0
28	37.56181144584186	40.0	35.0	41.0	33.0	41.0
29	37.50656332260895	40.0	35.0	41.0	33.0	41.0
30	37.40581846543824	40.0	35.0	41.0	33.0	41.0
31	37.39732085405089	40.0	35.0	41.0	33.0	41.0
32	37.29501413668714	40.0	35.0	41.0	33.0	41.0
33	37.16794384322901	39.0	35.0	41.0	32.0	41.0
34	37.06173540021449	39.0	35.0	41.0	32.0	41.0
35	36.994146436579896	39.0	35.0	41.0	31.0	41.0
36	36.92675441162133	39.0	35.0	41.0	31.0	41.0
37	36.88689285366092	39.0	35.0	41.0	31.0	41.0
38	36.801869942478305	39.0	35.0	41.0	31.0	41.0
39	36.66471482889734	39.0	35.0	41.0	31.0	41.0
40	36.4939670468948	39.0	35.0	41.0	30.0	41.0
41	36.31530466998148	39.0	35.0	41.0	30.0	41.0
42	36.3946300087745	39.0	35.0	41.0	30.0	41.0
43	36.33012576776835	39.0	35.0	41.0	30.0	41.0
44	36.27138149556401	39.0	35.0	41.0	30.0	41.0
45	36.265845763868576	39.0	35.0	40.0	30.0	41.0
46	36.12431120210588	38.0	35.0	40.0	30.0	41.0
47	35.945845763868576	38.0	35.0	40.0	30.0	41.0
48	35.86909622696695	38.0	35.0	40.0	29.0	41.0
49	35.847752754216636	38.0	35.0	40.0	29.0	41.0
50	35.71728380618114	38.0	35.0	40.0	29.0	41.0
51	34.43437067368626	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	13.0
10	14.0
11	17.0
12	8.0
13	12.0
14	11.0
15	25.0
16	60.0
17	77.0
18	133.0
19	257.0
20	576.0
21	1016.0
22	1429.0
23	1992.0
24	2665.0
25	3312.0
26	4000.0
27	4754.0
28	5474.0
29	6571.0
30	8292.0
31	10835.0
32	14282.0
33	20662.0
34	35953.0
35	40968.0
36	38520.0
37	58339.0
38	96856.0
39	155650.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09681193331384	20.382957979916156	23.200155991030517	11.320074095739495
2	31.0944720678561	28.003509798186606	23.559520327581165	17.342497806376134
3	30.504630983718435	27.728965584478892	25.37311104611485	16.393292385687825
4	28.00253485424588	23.944038217802476	29.273276786584773	18.78015014136687
5	27.013941698352344	29.1477040070196	24.29072828312372	19.547626011504338
6	25.0853075948133	35.08920737057619	24.922491956712488	14.902993077898023
7	75.8720873549771	6.164570537194111	12.831627181437067	5.131714926391733
8	75.97367651360047	8.301257677683534	9.63186116798284	6.093204640733158
9	69.5012186799259	7.5207175587403725	11.844203958272399	11.133859803061325
10	42.2217022521205	23.204250755581555	19.55484059666569	15.019206395632251
11	32.46446329336063	24.321536511650578	24.35195476260115	18.86204543238764
12	28.38373793506873	20.269669494004095	29.7525592278444	21.59403334308277
13	26.708589256117772	21.993370381203082	32.31607682558253	18.981963537096615
14	20.990543043774984	27.444086964999514	30.724773325533782	20.840596665691724
15	18.826362484157162	24.20239836209418	36.541678853465925	20.429560300282734
16	22.98293848103734	24.56098274349225	29.106756361509213	23.349322413961197
17	23.72311592083455	24.54967339377986	27.58389392609925	24.14331675928634
18	24.3464950765331	23.853953397679632	29.23408404016769	22.56546748561958
19	24.066881154333625	25.789997075168174	27.001267427122936	23.141854343375257
20	24.23769133274837	27.352442234571512	28.276104124012868	20.13376230866725
21	23.97328653602418	27.114945890611292	30.022423710636637	18.88934386272789
22	21.86721263527347	23.13366481427318	30.518865165252997	24.480257385200353
23	21.974651457541192	25.425173052549475	29.85668324071366	22.743492249195672
24	23.205420688310422	24.516135322218975	28.00818952910208	24.27025446036853
25	22.212537779077703	28.4490591790972	25.742419810860877	23.595983230964222
26	20.117968216827535	25.37447596763186	27.328068636053427	27.17948717948718
27	22.56546748561958	25.12411036365409	27.216340060446527	25.094082090279805
28	19.306814858145657	25.01745149653895	29.367261382470506	26.308472262844884
29	21.63810080920347	23.842644047967244	28.07526567222385	26.443989470605437
30	22.65672223847129	24.06376133372331	28.26908452763966	25.01043190016574
31	24.00370478697475	23.42185824315102	26.046797309154723	26.527639660719508
32	24.151116310812128	24.527249683143218	27.46066101199181	23.860972994052844
33	23.338598030613237	23.10032173150044	28.065126255240326	25.495953982645997
34	23.05742419810861	22.90338305547431	29.26235741444867	24.77683533196841
35	21.33976796334211	23.610412401286926	29.535731695427515	25.51408793994345
36	22.512625524032366	25.107341327873645	26.983913424978063	25.396119723115923
37	21.27698157355952	25.307399824510092	28.942965779467684	24.47265282246271
38	21.354977088817392	24.902408111533585	27.99590523544896	25.74670956420006
39	23.42068831042215	23.247148288973385	28.854635858438137	24.477527542166325
40	23.591693477625036	21.938968509310712	31.515842839036758	22.953495174027495
41	21.542166325436288	24.429170322706444	27.408793994345327	26.619869357511945
42	20.829092327191187	23.460856000779955	31.233889051379542	24.476162620649315
43	22.004484742127328	23.92453933898801	28.701959637320858	25.369016281563812
44	22.06863605342693	22.310812128302622	29.036365408988985	26.584186409281468
45	20.80354879594423	21.588378668226575	28.641903090572292	28.966169445256895
46	24.151701277176564	22.94472067856098	28.020668811543338	24.88290923271912
47	19.800331480939846	22.821877742029834	32.84274154236132	24.535049234669007
48	20.813493224139613	23.937213610217412	29.57433947548016	25.674953690162816
49	22.03802281368821	21.494004094764552	31.992785414838647	24.47518767670859
50	21.496733937798577	21.92083455201326	30.492736667641612	26.08969484254655
51	20.67602612849761	22.482207273081798	27.743979721166035	29.097786877254556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	359.0
2	547.0
3	1181.5
4	1816.0
5	1267.0
6	718.0
7	743.5
8	769.0
9	774.5
10	780.0
11	788.0
12	796.0
13	831.0
14	866.0
15	848.0
16	830.0
17	842.5
18	855.0
19	1027.5
20	1200.0
21	1327.0
22	1454.0
23	1549.0
24	1644.0
25	1874.5
26	2648.5
27	3192.0
28	3916.5
29	4641.0
30	5616.0
31	6591.0
32	8007.5
33	9424.0
34	10413.0
35	11402.0
36	13087.5
37	14773.0
38	15755.0
39	16737.0
40	18722.5
41	20708.0
42	23630.5
43	26553.0
44	28801.5
45	31050.0
46	45019.5
47	58989.0
48	55318.0
49	51647.0
50	50941.0
51	50235.0
52	43320.5
53	36406.0
54	32851.0
55	29296.0
56	27876.0
57	26456.0
58	25704.0
59	24952.0
60	22443.5
61	19935.0
62	18042.5
63	16150.0
64	14343.5
65	12537.0
66	10898.5
67	9260.0
68	7771.0
69	6282.0
70	5427.0
71	4572.0
72	3672.0
73	2772.0
74	2263.0
75	1402.5
76	1051.0
77	785.0
78	519.0
79	364.5
80	210.0
81	155.0
82	100.0
83	82.5
84	65.0
85	39.0
86	13.0
87	13.5
88	14.0
89	11.0
90	8.0
91	5.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	512850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.345404419751674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0248126557137	24.68387315101125
2	9.856129469375482	6.573132463395156
3	3.597882393566133	3.599185304045005
4	1.8506277543026473	2.4683972359055444
5	1.1624237761210954	1.9380745460946396
6	0.8460950919355256	1.692802981089424
7	0.6913041004439046	1.613627036443424
8	0.5965409159119711	1.5913518475211001
9	0.5161233909989956	1.5489308883019615
>10	6.738020590398731	45.574379847900175
>50	0.07826128207757846	1.7704442823981659
>100	0.033540549461819336	1.961164867845469
>500	0.004707445538500959	1.2316390070501262
>1k	0.0035305841538757193	3.752996540998528
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	4913	0.957979916154821	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	4793	0.9345812615774594	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	4230	0.8248025738520036	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	2367	0.46153846153846156	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	1443	0.28136882129277563	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	1381	0.2692795164278054	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	959	0.18699424783074972	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	930	0.18133957297455397	No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	914	0.17821975236423906	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	834	0.1626206493126645	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	818	0.1595008287023496	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT	651	0.12693770108218777	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC	610	0.11894316076825583	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA	561	0.10938871014916643	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.84966364434045E-4	0.0	0.0	0.1914789899580774	0.0
2	5.84966364434045E-4	0.0	0.0	1.1711026615969582	0.0
3	5.84966364434045E-4	0.0	0.0	1.6074875694647557	0.0
4	5.84966364434045E-4	0.0	0.0	2.2727893146144096	0.0
5	5.84966364434045E-4	0.0	0.0	4.5740469922979425	0.0
6	5.84966364434045E-4	0.0	0.0	5.719411133859803	0.0
7	5.84966364434045E-4	0.0	0.0	6.685385590328556	0.0
8	7.799551525787267E-4	0.0	0.0	7.9910305157453445	0.0
9	7.799551525787267E-4	0.0	0.0	8.432095154528614	0.0
10	7.799551525787267E-4	0.0	0.0	10.18660427025446	0.0
11	7.799551525787267E-4	0.0	0.0	11.547821000292483	0.0
12	7.799551525787267E-4	0.0	0.0	13.6260115043385	0.0
13	7.799551525787267E-4	0.0	0.0	14.203178317246758	0.0
14	7.799551525787267E-4	0.0	0.0	14.473627766403432	0.0
15	7.799551525787267E-4	0.0	0.0	14.95583503948523	0.0
16	7.799551525787267E-4	0.0	0.0	15.65311494589061	0.0
17	7.799551525787267E-4	0.0	0.0	16.42390562542654	0.0
18	7.799551525787267E-4	0.0	0.0	17.307984790874524	0.0
19	7.799551525787267E-4	0.0	0.0	17.952422735692696	0.0
20	7.799551525787267E-4	0.0	0.0	18.543823730135518	0.0
21	7.799551525787267E-4	0.0	0.0	19.17246758311397	0.0
22	7.799551525787267E-4	0.0	0.0	19.860193038900263	0.0
23	7.799551525787267E-4	0.0	0.0	20.493321634006044	0.0
24	7.799551525787267E-4	0.0	0.0	21.022716193818855	0.0
25	7.799551525787267E-4	0.0	0.0	21.487179487179485	0.0
26	7.799551525787267E-4	0.0	0.0	21.965291995710245	0.0
27	9.749439407234084E-4	0.0	0.0	22.527834649507653	0.0
28	9.749439407234084E-4	0.0	0.0	22.982353514672905	0.0
29	9.749439407234084E-4	0.0	0.0	23.496538949010432	0.0
30	9.749439407234084E-4	0.0	0.0	24.164375548405967	0.0
31	9.749439407234084E-4	0.0	0.0	24.63215365116506	0.0
32	9.749439407234084E-4	0.0	0.0	25.099346787559714	0.0
33	9.749439407234084E-4	0.0	0.0	25.565954957589938	0.0
34	0.00116993272886809	0.0	0.0	26.063371356147023	0.0
35	0.00116993272886809	0.0	0.0	26.576581846543824	0.0
36	0.00116993272886809	0.0	0.0	27.02993077898021	0.0
37	0.00116993272886809	0.0	0.0	27.480939845958858	0.0
38	0.00116993272886809	0.0	0.0	28.00253485424588	0.0
39	0.00116993272886809	0.0	0.0	28.78307497318904	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	60	0.0	45.000004	4
CGGTCTA	30	2.1631422E-6	45.000004	31
CTCGTAG	25	3.8877737E-5	45.0	1
TGCGATT	20	7.029629E-4	45.0	38
ATGACGG	20	7.029629E-4	45.0	2
TATCGAT	20	7.029629E-4	45.0	16
CTTACGG	25	3.8877737E-5	45.0	2
TTACGGG	110	0.0	40.909092	3
TCGATCA	50	1.0786607E-9	40.5	17
GCCTCGA	50	1.0786607E-9	40.5	14
CAACGAG	85	0.0	39.705883	14
CTACGGG	85	0.0	39.705883	3
TTCGCAC	40	3.453224E-7	39.375	43
TCAACGG	40	3.453224E-7	39.375	2
CACGACG	35	6.2415256E-6	38.571426	26
GCCGATA	35	6.2415256E-6	38.571426	9
TTAAGCG	35	6.2415256E-6	38.571426	1
CGTTTTT	605	0.0	37.561985	1
CAACGGG	120	0.0	37.500004	3
TAGACGG	60	1.546141E-10	37.500004	2
>>END_MODULE