##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936441.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1075356
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05582058406704	33.0	31.0	34.0	30.0	34.0
2	32.070976495225764	33.0	31.0	34.0	30.0	34.0
3	32.107770821941756	33.0	31.0	34.0	30.0	34.0
4	35.77829481585633	37.0	35.0	37.0	35.0	37.0
5	34.907204683844235	37.0	35.0	37.0	33.0	37.0
6	35.35268320444578	37.0	35.0	37.0	33.0	37.0
7	35.91515088956587	37.0	35.0	37.0	35.0	37.0
8	35.91750545865741	37.0	35.0	37.0	35.0	37.0
9	37.7555907067055	39.0	38.0	39.0	35.0	39.0
10	37.4103896756051	39.0	37.0	39.0	34.0	39.0
11	37.30630042516153	39.0	37.0	39.0	34.0	39.0
12	37.12272772923571	39.0	37.0	39.0	34.0	39.0
13	36.985344388277	39.0	37.0	39.0	33.0	39.0
14	37.80078969197177	40.0	37.0	41.0	33.0	41.0
15	38.01333883848697	40.0	37.0	41.0	33.0	41.0
16	38.1100733152556	40.0	37.0	41.0	33.0	41.0
17	38.1536570214887	40.0	37.0	41.0	33.0	41.0
18	38.16069841057287	40.0	37.0	41.0	34.0	41.0
19	38.0886869092654	40.0	37.0	41.0	34.0	41.0
20	38.07448882044644	40.0	36.0	41.0	34.0	41.0
21	37.98695129798876	40.0	36.0	41.0	33.0	41.0
22	38.05621952172118	40.0	36.0	41.0	34.0	41.0
23	38.0905355993736	40.0	36.0	41.0	34.0	41.0
24	38.0440198408713	40.0	36.0	41.0	34.0	41.0
25	37.87028202753321	40.0	36.0	41.0	34.0	41.0
26	37.893739375611425	40.0	36.0	41.0	34.0	41.0
27	37.88941894591186	40.0	36.0	41.0	34.0	41.0
28	37.795752290404295	40.0	36.0	41.0	34.0	41.0
29	37.72348041020834	40.0	35.0	41.0	33.0	41.0
30	37.59918296824493	40.0	35.0	41.0	33.0	41.0
31	37.5400044264411	40.0	35.0	41.0	33.0	41.0
32	37.485845617637324	40.0	35.0	41.0	33.0	41.0
33	37.36853190943278	40.0	35.0	41.0	33.0	41.0
34	37.2309486346847	39.0	35.0	41.0	32.0	41.0
35	37.13029824541826	39.0	35.0	41.0	32.0	41.0
36	37.0649691822987	39.0	35.0	41.0	32.0	41.0
37	36.96780787013789	39.0	35.0	41.0	31.0	41.0
38	36.91379691934578	39.0	35.0	41.0	31.0	41.0
39	36.840916868460305	39.0	35.0	41.0	31.0	41.0
40	36.67788713691094	39.0	35.0	41.0	31.0	41.0
41	36.58431626363735	39.0	35.0	41.0	31.0	41.0
42	36.59380242449942	39.0	35.0	41.0	31.0	41.0
43	36.47434617001254	39.0	35.0	41.0	31.0	41.0
44	36.32428888665707	39.0	35.0	41.0	31.0	41.0
45	36.322684766719114	38.0	35.0	41.0	31.0	41.0
46	36.26262465639286	38.0	35.0	40.0	31.0	41.0
47	36.16914770550404	38.0	35.0	40.0	31.0	41.0
48	36.11488288529566	38.0	35.0	40.0	30.0	41.0
49	36.08818660982968	38.0	35.0	40.0	30.0	41.0
50	35.954752658654435	38.0	35.0	40.0	30.0	41.0
51	34.78552218986085	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	23.0
10	35.0
11	19.0
12	34.0
13	31.0
14	23.0
15	52.0
16	109.0
17	157.0
18	306.0
19	449.0
20	806.0
21	1226.0
22	1868.0
23	2684.0
24	4105.0
25	5687.0
26	7576.0
27	9116.0
28	10783.0
29	13078.0
30	16256.0
31	21380.0
32	28612.0
33	43119.0
34	78702.0
35	84695.0
36	80826.0
37	121321.0
38	210391.0
39	331643.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72627483363649	22.376496713646457	23.943605652453698	11.953622800263354
2	31.99070819337968	24.838100126841717	25.412700538240358	17.758491141538244
3	31.795517019480062	23.87944085493548	27.76159708970797	16.56344503587649
4	29.540449860325324	25.169525254892335	26.51187141746547	18.77815346731687
5	25.416606221567555	30.63766789788684	25.356440099836707	18.5892857807089
6	26.972463072694065	32.388157968151944	25.084158176455052	15.555220782698939
7	79.1542521732338	6.16586507165999	9.538236639773247	5.141646115332969
8	80.11272546021968	5.024940577817951	9.302128783398242	5.5602051785641216
9	73.74990235791682	7.105925851531958	11.940324878458854	7.203846912092368
10	42.94698685830553	22.468745234136417	19.373305212413378	15.210962695144678
11	31.63947567131257	24.450600545307786	24.697402534602496	19.212521248777147
12	29.37427233399916	21.134117445757497	29.945618009291806	19.545992210951535
13	27.044253251946333	22.074736180390495	31.12104270585741	19.759967861805766
14	20.969706776174586	24.90412477356336	32.506816347330556	21.619352102931494
15	19.669114228218376	24.34672796729641	35.19550734826421	20.78865045622101
16	25.277303516230905	24.12605685930985	29.51171519013238	21.084924434326865
17	25.510342621420257	23.648726561250413	27.306770967009996	23.534159850319337
18	25.901468908900867	23.621386777960044	30.031357057569775	20.445787255569318
19	25.585108559398005	25.345931951837347	27.3985545251991	21.670404963565552
20	24.150048914034052	28.584952332064915	27.284545769029044	19.980452984871985
21	24.359281949419543	25.53154490233932	30.774738784179377	19.33443436406176
22	21.94994029884057	23.881858658899937	30.807658115080027	23.360542927179466
23	22.12179036523719	23.60873980337674	30.941753242647085	23.327716588738983
24	23.47910831389791	25.181614274714608	28.714955791384433	22.624321620003048
25	23.628361212472893	25.4952778428725	26.825256008242853	24.051104936411754
26	20.530782364165916	26.798939142014365	27.276920387295	25.393358106524722
27	21.07980984901744	26.070343216572	29.83123728328107	23.018609651129488
28	19.066802063688677	26.365966247456658	29.37724809272464	25.189983596130027
29	21.333586272825002	28.226652383024785	28.00105267464914	22.438708669501075
30	24.097415181577077	26.635086427192483	27.41957082119782	21.84792757003262
31	23.79463173125923	26.874635004593827	28.17894725095689	21.151786013190048
32	25.818984596728896	27.47713315404387	25.26781828529343	21.436063963933805
33	26.723429264355246	25.285858822566667	26.51717198769524	21.473539925382852
34	23.512399614639246	25.49946250358021	29.176663356135084	21.811474525645462
35	23.84642853157466	27.812649950342028	26.76629878849423	21.574622729589084
36	26.434315705682582	27.451188257656067	25.83758308876316	20.276912947898186
37	23.580935057785517	28.131800073650027	27.672696297784178	20.614568570780282
38	25.047798124528065	27.171838907301392	26.08438507805787	21.69597789011267
39	25.61272732006889	24.838658081602745	26.052860634059787	23.495753964268577
40	27.168305193814884	23.44423614133366	27.516096994855655	21.8713616699958
41	23.97940774961966	24.272984946380546	26.55985552691388	25.187751777085914
42	23.216497606374077	24.722603491308924	28.869230282808672	23.19166861950833
43	22.834391587530085	24.502862307924072	29.293461886110272	23.369284218435567
44	22.787616380063906	24.00711950275072	28.006353244878905	25.198910872306474
45	22.828440070079118	24.10783033711627	26.630250819263573	26.43347877354104
46	24.696379617540607	24.5566119499031	27.69380558624307	23.05320284631322
47	21.92399540245277	25.074207983216724	30.578710678138215	22.423085936192294
48	22.540070451087825	25.264005594426404	28.746201258002003	23.449722696483768
49	24.689033213187077	23.36844728629403	29.548912174200915	22.393607326317984
50	23.28103437373298	23.381001268417158	28.516788858759334	24.821175499090533
51	22.270020346750286	24.052034861013468	26.67172545649999	27.006219335736258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	497.0
1	961.5
2	1426.0
3	2938.0
4	4450.0
5	3081.0
6	1712.0
7	1737.5
8	1763.0
9	1789.0
10	1815.0
11	1866.5
12	1918.0
13	2033.0
14	2148.0
15	2156.5
16	2165.0
17	2281.5
18	2398.0
19	2378.0
20	2358.0
21	2872.5
22	3387.0
23	3712.0
24	4037.0
25	4690.0
26	6575.0
27	7807.0
28	9176.0
29	10545.0
30	12654.0
31	14763.0
32	17759.0
33	20755.0
34	23418.0
35	26081.0
36	28772.0
37	31463.0
38	34286.0
39	37109.0
40	41703.5
41	46298.0
42	50949.0
43	55600.0
44	61273.5
45	66947.0
46	78560.5
47	90174.0
48	101317.5
49	112461.0
50	111482.0
51	110503.0
52	95096.0
53	79689.0
54	72418.5
55	65148.0
56	61335.5
57	57523.0
58	54213.0
59	50903.0
60	46026.5
61	41150.0
62	37968.5
63	34787.0
64	30042.0
65	25297.0
66	22469.0
67	19641.0
68	16370.0
69	13099.0
70	10609.0
71	8119.0
72	7118.0
73	6117.0
74	4992.0
75	2965.0
76	2063.0
77	1573.5
78	1084.0
79	750.5
80	417.0
81	379.5
82	342.0
83	222.0
84	102.0
85	75.5
86	49.0
87	35.0
88	21.0
89	14.5
90	8.0
91	5.0
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1075356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.419247131720187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.39991356433883	21.005317023327446
2	11.94718186980399	7.02954191910741
3	4.4810213247779505	3.9548482126845217
4	2.1513634741792136	2.5316597486813768
5	1.2914178165098034	1.899626994710419
6	0.8526313809552768	1.5050263985149892
7	0.6303958008576395	1.2982038899360695
8	0.46047028948949276	1.08373513946449
9	0.4158273621998899	1.1009995139420896
>10	5.710208673512155	42.260234955027734
>50	0.5932627671146192	10.614430059845384
>100	0.061229643628728715	3.4228070331100597
>500	0.0019035122371552106	0.346172696146437
>1k	0.0031725203952586846	1.9473964155015924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	3832	0.3563471073765339	No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	3536	0.32882133916582046	No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT	2719	0.2528464992058444	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2414	0.22448379885358893	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2413	0.22439080639341763	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCC	1360	0.12646974583300785	No Hit
TCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	1316	0.12237807758546937	No Hit
CGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG	1161	0.10796424625891332	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1085	0.1008968192858923	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.789773805139879E-4	0.0	0.0	0.26633040593068713	0.0
2	2.789773805139879E-4	0.0	0.0	1.2235017984741796	0.0
3	2.789773805139879E-4	0.0	0.0	1.7199885433289068	0.0
4	2.789773805139879E-4	0.0	0.0	2.3143963487440438	0.0
5	2.789773805139879E-4	0.0	0.0	3.8736939208968937	0.0
6	2.789773805139879E-4	0.0	0.0	4.7399186873928265	0.0
7	2.789773805139879E-4	0.0	0.0	5.619999330454287	0.0
8	2.789773805139879E-4	0.0	0.0	6.816998277779637	0.0
9	2.789773805139879E-4	0.0	0.0	7.317390705961561	0.0
10	2.789773805139879E-4	0.0	0.0	8.413957796301876	0.0
11	2.789773805139879E-4	0.0	0.0	10.045417517547678	0.0
12	2.789773805139879E-4	0.0	0.0	11.257295258500442	0.0
13	2.789773805139879E-4	0.0	0.0	11.755548860098424	0.0
14	2.789773805139879E-4	0.0	0.0	11.996027362101481	0.0
15	2.789773805139879E-4	0.0	0.0	12.413935478111435	0.0
16	2.789773805139879E-4	0.0	0.0	13.196931992754028	0.0
17	2.789773805139879E-4	0.0	0.0	14.105747306008428	0.0
18	2.789773805139879E-4	0.0	0.0	15.103742388567134	0.0
19	2.789773805139879E-4	0.0	0.0	15.731069524882923	0.0
20	2.789773805139879E-4	0.0	0.0	16.399220351213923	0.0
21	2.789773805139879E-4	0.0	0.0	17.16640814762739	0.0
22	2.789773805139879E-4	0.0	0.0	17.99748176417856	0.0
23	2.789773805139879E-4	0.0	0.0	18.729983372948123	0.0
24	3.7196984068531725E-4	0.0	0.0	19.337689100167758	0.0
25	3.7196984068531725E-4	0.0	0.0	19.895178898894876	0.0
26	3.7196984068531725E-4	0.0	0.0	20.4374179341539	0.0
27	3.7196984068531725E-4	0.0	0.0	21.097385423989824	0.0
28	4.6496230085664653E-4	0.0	0.0	21.667708182220586	0.0
29	4.6496230085664653E-4	0.0	0.0	22.285364102678553	0.0
30	4.6496230085664653E-4	0.0	0.0	22.99573350592734	0.0
31	4.6496230085664653E-4	0.0	0.0	23.570705887166667	0.0
32	4.6496230085664653E-4	0.0	0.0	24.104668593470443	0.0
33	4.6496230085664653E-4	0.0	0.0	24.66708699258664	0.0
34	4.6496230085664653E-4	0.0	0.0	25.261494798001777	0.0
35	5.579547610279758E-4	0.0	0.0	25.827818880445175	0.0
36	5.579547610279758E-4	0.0	0.0	26.355644084377637	0.0
37	5.579547610279758E-4	0.0	0.0	26.90485755414951	0.0
38	5.579547610279758E-4	0.0	0.0	27.534695486889923	0.0
39	5.579547610279758E-4	0.0	0.0	28.483590550478166	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	25	3.8906368E-5	45.000004	1
CCGTAAG	20	7.033084E-4	45.0	1
ATCGATC	20	7.033084E-4	45.0	28
TACGACG	30	2.1653705E-6	44.999996	1
TTAACGG	70	0.0	41.785717	2
TAGCCGT	60	3.6379788E-12	41.249996	44
GACGTAG	45	1.9281288E-8	40.0	1
CGTTTTT	1480	0.0	39.83108	1
TAACGGG	345	0.0	39.130432	3
ACGTTAG	35	6.247914E-6	38.57143	1
ATCGTAA	35	6.247914E-6	38.57143	21
TACGATC	35	6.247914E-6	38.57143	11
TCACGAC	65	9.094947E-12	38.076927	25
AGGGATC	625	0.0	37.8	6
TAGTTCG	30	1.13987306E-4	37.499996	1
AACGGTA	30	1.13987306E-4	37.499996	24
TACGCGG	60	1.546141E-10	37.499996	2
TTACGCG	30	1.13987306E-4	37.499996	1
ACGCGCA	30	1.13987306E-4	37.499996	15
ATAACGG	115	0.0	37.173912	2
>>END_MODULE