FastQCFastQC Report
Sat 14 Jan 2017
SRR2936440.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936440.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337752
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208071.5553705021558555No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC72080.5388143691805357Illumina PCR Primer Index 11 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT66750.4989714087513979No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC52980.39603753162021066Illumina PCR Primer Index 11 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG49450.3696499799663914Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG46270.3458787577966619No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC43910.3282372218467997No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT38270.2860769410174681No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC29450.22014543801840697Illumina PCR Primer Index 11 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT28680.2143895131534096Illumina PCR Primer Index 11 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC27250.20369993840412873Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT21600.16146490530382313Illumina PCR Primer Index 11 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT18870.14105753532792326Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC17370.12984469468182444No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC16470.12311699029416515No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG253.89115E-545.01
ACGTACG207.0337026E-445.01
CGTTTTT112900.043.9836161
TTGTGCG1400.040.1785741
CGTTATT4050.040.01
CGTTTCT8850.039.406781
GCTACGA1100.038.86363210
CGCGGGT1250.037.84
CTACGAA1250.037.811
GTTTTTT132500.037.5113222
AACCCGC601.5643309E-1037.50000421
CGATCGT301.1400218E-437.50000410
CGGGTAT1150.037.1739126
CGTTCTG6900.037.1739121
CGTTAGG1800.036.252
CGGTCTA751.8189894E-1236.031
CGTTTGG1250.036.02
TCGGTCG250.002107231936.030
TAGTACG250.002107231936.01
TACGAAT1400.035.35714312
Produced by FastQC (version 0.11.5)