##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936437.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382970
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97459853252213	33.0	31.0	34.0	30.0	34.0
2	32.0895553176489	33.0	31.0	34.0	30.0	34.0
3	32.07069222132282	33.0	31.0	34.0	30.0	34.0
4	35.697717836906286	37.0	35.0	37.0	33.0	37.0
5	31.82711439538345	37.0	35.0	37.0	0.0	37.0
6	33.705402511946104	37.0	35.0	37.0	17.0	37.0
7	35.402175105099616	37.0	35.0	37.0	32.0	37.0
8	35.61696216413819	37.0	35.0	37.0	33.0	37.0
9	37.572681933310704	39.0	37.0	39.0	35.0	39.0
10	37.21310285400945	39.0	37.0	39.0	34.0	39.0
11	37.21683682794997	39.0	37.0	39.0	34.0	39.0
12	37.103770530328745	39.0	37.0	39.0	34.0	39.0
13	36.93724051492284	39.0	37.0	39.0	33.0	39.0
14	38.10812074052798	40.0	37.0	41.0	33.0	41.0
15	38.20010183565292	40.0	37.0	41.0	33.0	41.0
16	38.26045904378933	40.0	37.0	41.0	34.0	41.0
17	38.1970310990417	40.0	37.0	41.0	34.0	41.0
18	38.15208763088493	40.0	37.0	41.0	34.0	41.0
19	38.021234039219785	40.0	37.0	41.0	34.0	41.0
20	37.98934120166071	40.0	36.0	41.0	33.0	41.0
21	38.03480951510562	40.0	37.0	41.0	34.0	41.0
22	38.10254589132308	40.0	37.0	41.0	34.0	41.0
23	38.07871373736846	40.0	37.0	41.0	34.0	41.0
24	38.06323732929472	40.0	36.0	41.0	34.0	41.0
25	37.88645324699063	40.0	36.0	41.0	33.0	41.0
26	37.86206230253023	40.0	36.0	41.0	33.0	41.0
27	37.810483849909915	40.0	36.0	41.0	33.0	41.0
28	37.72878554455963	40.0	36.0	41.0	33.0	41.0
29	37.7032039063112	40.0	36.0	41.0	33.0	41.0
30	37.59140663759563	40.0	36.0	41.0	33.0	41.0
31	37.5311408204298	40.0	36.0	41.0	33.0	41.0
32	37.42512207222498	40.0	36.0	41.0	33.0	41.0
33	37.35879311695433	40.0	35.0	41.0	33.0	41.0
34	37.1814815781915	40.0	35.0	41.0	32.0	41.0
35	37.19108285244275	40.0	35.0	41.0	32.0	41.0
36	37.11919210382014	40.0	35.0	41.0	32.0	41.0
37	36.96521920777084	40.0	35.0	41.0	32.0	41.0
38	36.813283024780006	39.0	35.0	41.0	31.0	41.0
39	36.785852677755436	39.0	35.0	41.0	31.0	41.0
40	36.66153745724208	39.0	35.0	41.0	31.0	41.0
41	36.59345640650704	39.0	35.0	41.0	31.0	41.0
42	36.536391884481816	39.0	35.0	41.0	31.0	41.0
43	36.47321461211061	39.0	35.0	41.0	31.0	41.0
44	36.436493720134735	39.0	35.0	41.0	31.0	41.0
45	36.47117528788156	39.0	35.0	41.0	31.0	41.0
46	36.392001984489646	39.0	35.0	41.0	31.0	41.0
47	36.2468809567329	39.0	35.0	41.0	30.0	41.0
48	36.24379716426874	39.0	35.0	41.0	30.0	41.0
49	36.22239601013134	39.0	35.0	40.0	30.0	41.0
50	36.09072512207222	38.0	35.0	40.0	30.0	41.0
51	35.01838525210852	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	12.0
10	15.0
11	17.0
12	4.0
13	12.0
14	9.0
15	16.0
16	26.0
17	44.0
18	126.0
19	227.0
20	538.0
21	946.0
22	1316.0
23	1685.0
24	2118.0
25	2521.0
26	2921.0
27	3173.0
28	3542.0
29	4274.0
30	5544.0
31	7382.0
32	9845.0
33	14895.0
34	24314.0
35	32659.0
36	29438.0
37	45516.0
38	73283.0
39	116451.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.84855210590908	18.79990599785884	20.583335509308824	10.768206386923259
2	31.816069143797165	27.976081677415987	22.223150638431207	17.98469854035564
3	30.992767057471866	28.379507533227144	24.703762696816984	15.923962712484007
4	27.16792438049978	24.286758753949396	29.733399482988222	18.811917382562605
5	24.96487975559443	36.06496592422383	20.762461811630153	18.207692508551585
6	23.536830561140558	38.56228947437136	23.79794761991801	14.102932344570071
7	75.83570514661723	5.44637961198005	13.767919158158604	4.949996083244118
8	75.51635898373242	9.39681959422409	9.49212732067786	5.594694101365643
9	71.0363736062877	6.382484267697208	10.34806903935034	12.233073086664751
10	40.191398804083875	24.70480716505209	19.286628195420008	15.81716583544403
11	32.876465519492385	24.154894639266782	23.1067707653341	19.86186907590673
12	27.676058176880698	21.135075854505576	29.218998877196643	21.969867091417083
13	25.379794761991803	24.564326187429824	31.792568608507192	18.26331044207118
14	20.721205316343315	30.292973339948297	28.075045042692636	20.910776301015748
15	18.162258140324308	24.62229417447842	36.4508969370969	20.764550748100376
16	20.97370551218111	24.166906023970547	30.228999660547824	24.63038880330052
17	21.43666605739353	23.839465232263624	29.466537848917667	25.257330861425174
18	22.97046766065227	24.017808183408622	29.954826748831504	23.056897407107606
19	22.438311094863828	25.89419536778338	27.455936496331308	24.21155704102149
20	25.47196908374024	25.64404522547458	29.547745254197455	19.336240436587723
21	22.797085933624047	29.5900462177194	27.720970310990417	19.891897537666136
22	20.763245162806488	23.609421103480692	29.235710368958408	26.39162336475442
23	20.95098832806747	28.01864375799671	28.141368775622112	22.888999138313707
24	22.100686737864585	25.431234822570957	28.542444577904273	23.92563386166018
25	20.631381048123874	29.597618612423947	26.236258714781833	23.53474162467034
26	20.181998589967883	25.91743478601457	29.343029480115934	24.557537143901612
27	23.95879572812492	26.743348042927646	26.744392511162758	22.553463717784684
28	18.626002036713057	27.68859179570201	30.068151552340915	23.61725461524401
29	21.28626263153772	27.021959944643182	27.74368749510405	23.948089928715042
30	23.504190928793378	28.695198057289083	27.08488915580855	20.71572185810899
31	24.033736323994045	28.096978875629947	23.621432488184453	24.247852312191558
32	24.758597279160245	29.274616810716243	23.15168289944382	22.81510301067969
33	24.596704702718228	27.69094184923101	23.266052171188345	24.446301276862417
34	24.054625688696245	27.536621667493538	25.299892942005904	23.10885970180432
35	23.30313079353474	28.237720970310992	24.796981486800533	23.662166749353737
36	25.0787267932214	30.79014021986056	24.057497976342795	20.07363501057524
37	24.345248975115545	27.23816486931091	25.959735749536517	22.456850406037027
38	21.307413113298693	31.478705903856696	22.903099459487688	24.31078152335692
39	25.06332088675353	26.160012533618822	24.469018460976056	24.307648118651592
40	23.29111940883098	27.617829072773326	27.068961015223124	22.022090503172574
41	23.871843747552028	27.363762174582867	24.35177690158498	24.412617176280126
42	23.08222576180902	26.91881870642609	26.964253074653367	23.03470245711152
43	26.181163015379795	25.28474815259681	24.868788677964332	23.665300154059064
44	23.076481186515917	25.049481682638326	26.992975951118886	24.88106117972687
45	21.64738752382693	25.3925894978719	25.488419458443218	27.47160351985795
46	25.603572081364074	26.851711622320284	25.306943102592893	22.237773193722745
47	20.810246233386426	27.034754680523278	29.951171110008616	22.203827976081676
48	22.790296890095828	25.90829568895736	26.584327754132175	24.717079666814634
49	22.305663629004883	23.82327597461942	30.931404548659163	22.93965584771653
50	22.51194610543907	24.114160378097502	28.457320416742824	24.916573099720605
51	21.822074836149046	23.999268872235426	25.349505183173616	28.829151108441913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	129.0
1	354.5
2	580.0
3	930.0
4	1280.0
5	912.5
6	545.0
7	548.0
8	551.0
9	563.5
10	576.0
11	596.5
12	617.0
13	611.5
14	606.0
15	616.5
16	627.0
17	655.0
18	683.0
19	727.5
20	772.0
21	834.0
22	896.0
23	1084.5
24	1273.0
25	1457.0
26	2206.5
27	2772.0
28	3196.0
29	3620.0
30	4307.5
31	4995.0
32	5817.5
33	6640.0
34	7278.0
35	7916.0
36	9015.0
37	10114.0
38	11187.0
39	12260.0
40	13863.0
41	15466.0
42	18168.0
43	20870.0
44	24479.0
45	28088.0
46	38259.5
47	48431.0
48	43679.5
49	38928.0
50	39030.5
51	39133.0
52	34084.0
53	29035.0
54	26175.5
55	23316.0
56	20827.0
57	18338.0
58	17074.0
59	15810.0
60	14450.0
61	13090.0
62	11973.5
63	10857.0
64	9489.0
65	8121.0
66	6649.5
67	5178.0
68	4125.0
69	3072.0
70	2830.0
71	2588.0
72	2097.5
73	1607.0
74	1204.0
75	736.5
76	672.0
77	452.5
78	233.0
79	169.0
80	105.0
81	98.5
82	92.0
83	54.0
84	16.0
85	12.0
86	8.0
87	6.5
88	5.0
89	10.0
90	15.0
91	8.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	382970.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.32945720059354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72390316507762	24.965721211656412
2	10.556716880421451	7.248127206504231
3	3.982014675751761	4.101012071500663
4	2.1270370594209056	2.9208011078186527
5	1.2770633264644857	2.1920445404155084
6	0.9159863141741643	1.8867187781262835
7	0.7236384487801705	1.738948060927193
8	0.6226569593319171	1.7100380348829405
9	0.5194877607450232	1.6050359563855607
>10	6.44225297995906	42.72383100553928
>50	0.0741015083756888	1.679737372021676
>100	0.02444585843321692	1.425875424150172
>500	0.007639330760380287	1.8286976885413186
>1k	0.003055732304152115	3.973411541530119
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGC	4550	1.1880826174373973	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCG	4268	1.1144476068621563	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTC	4220	1.1019139880408388	No Hit
GCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	2113	0.5517403451967517	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCT	926	0.24179439642791867	No Hit
CCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	910	0.23761652348747944	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACAGAGT	856	0.22351620231349714	No Hit
CTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGCT	715	0.1866986970258767	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTC	649	0.169464971146565	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTA	627	0.1637203958534611	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	606	0.15823693761913465	No Hit
GAACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCT	601	0.1569313523252474	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCG	566	0.14779225526803666	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGC	517	0.13499751938794163	No Hit
GAATATGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTC	395	0.10314123821709273	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.222341175548998E-4	0.0	0.0	0.19688226231819725	0.0
2	5.222341175548998E-4	0.0	0.0	1.2008773533174921	0.0
3	5.222341175548998E-4	0.0	0.0	1.5452907538449487	0.0
4	5.222341175548998E-4	0.0	0.0	2.091025406689819	0.0
5	5.222341175548998E-4	0.0	0.0	4.325404078648458	0.0
6	5.222341175548998E-4	0.0	0.0	5.123638927331123	0.0
7	5.222341175548998E-4	0.0	0.0	6.040420920698749	0.0
8	5.222341175548998E-4	0.0	0.0	7.320155625767032	0.0
9	5.222341175548998E-4	0.0	0.0	7.746559782750607	0.0
10	5.222341175548998E-4	0.0	0.0	9.989816434707679	0.0
11	5.222341175548998E-4	0.0	0.0	11.37608689975716	0.0
12	5.222341175548998E-4	0.0	0.0	13.818314750502651	0.0
13	5.222341175548998E-4	0.0	0.0	14.359610413348305	0.0
14	5.222341175548998E-4	0.0	0.0	14.58834895683735	0.0
15	5.222341175548998E-4	0.0	0.0	15.116588766744131	0.0
16	5.222341175548998E-4	0.0	0.0	15.926051648954227	0.0
17	5.222341175548998E-4	0.0	0.0	16.82246651173721	0.0
18	5.222341175548998E-4	0.0	0.0	17.8126223986213	0.0
19	5.222341175548998E-4	0.0	0.0	18.608768310833746	0.0
20	5.222341175548998E-4	0.0	0.0	19.15215291014962	0.0
21	5.222341175548998E-4	0.0	0.0	19.775178212392614	0.0
22	5.222341175548998E-4	0.0	0.0	20.51596730814424	0.0
23	5.222341175548998E-4	0.0	0.0	21.216805493902918	0.0
24	5.222341175548998E-4	0.0	0.0	21.76410684910045	0.0
25	5.222341175548998E-4	0.0	0.0	22.255268036660834	0.0
26	5.222341175548998E-4	0.0	0.0	22.738595712457894	0.0
27	7.833511763323498E-4	0.0	0.0	23.206517481787085	0.0
28	7.833511763323498E-4	0.0	0.0	23.657988876413295	0.0
29	7.833511763323498E-4	0.0	0.0	24.124082826331044	0.0
30	7.833511763323498E-4	0.0	0.0	24.68235109799723	0.0
31	0.0010444682351097997	0.0	0.0	25.177690158498056	0.0
32	0.0010444682351097997	0.0	0.0	25.67381257017521	0.0
33	0.0010444682351097997	0.0	0.0	26.141995456563176	0.0
34	0.0010444682351097997	0.0	0.0	26.602605948246598	0.0
35	0.0010444682351097997	0.0	0.0	27.091678199336762	0.0
36	0.0013055852938872496	0.0	0.0	27.538971721022534	0.0
37	0.0013055852938872496	0.0	0.0	27.984176306238087	0.0
38	0.0013055852938872496	0.0	0.0	28.452098075567278	0.0
39	0.0013055852938872496	0.0	0.0	28.95944852077186	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	35	1.2090823E-7	45.000004	5
CCCGTAA	35	1.2090823E-7	45.000004	41
TTAGGCG	20	7.02739E-4	45.0	1
AGACTCG	20	7.02739E-4	45.0	12
ACGTTTA	20	7.02739E-4	45.0	24
CGTTGAT	20	7.02739E-4	45.0	25
GTTAGTC	20	7.02739E-4	45.0	8
TATGCGG	25	3.8859176E-5	45.0	2
CCGTCGA	25	3.8859176E-5	45.0	41
CCCGTAG	20	7.02739E-4	45.0	19
TCTACGG	20	7.02739E-4	45.0	2
CTACACG	20	7.02739E-4	45.0	1
CGTCGAA	25	3.8859176E-5	45.0	42
AGTAGTC	20	7.02739E-4	45.0	15
AGTAGCG	25	3.8859176E-5	45.0	1
CGTTTGA	20	7.02739E-4	45.0	19
TACACGG	25	3.8859176E-5	45.0	2
GCGTAAG	40	6.7939254E-9	45.0	1
TCGAAAG	20	7.02739E-4	45.0	1
TAATACG	125	0.0	43.199997	4
>>END_MODULE