##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936436.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	681210
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.778793617239913	31.0	31.0	34.0	30.0	34.0
2	31.887200716372337	33.0	31.0	34.0	30.0	34.0
3	31.894430498671483	33.0	31.0	34.0	30.0	34.0
4	35.58160185552179	37.0	35.0	37.0	33.0	37.0
5	31.855749328400933	37.0	35.0	37.0	0.0	37.0
6	33.62730582346119	37.0	35.0	37.0	17.0	37.0
7	35.2111008352784	37.0	35.0	37.0	32.0	37.0
8	35.351856255780156	37.0	35.0	37.0	33.0	37.0
9	37.19058293330985	39.0	37.0	39.0	34.0	39.0
10	36.91444928876558	39.0	37.0	39.0	33.0	39.0
11	36.901914240836156	39.0	37.0	39.0	33.0	39.0
12	36.75341818235199	39.0	35.0	39.0	33.0	39.0
13	36.50101290350993	39.0	35.0	39.0	32.0	39.0
14	37.516128653425525	40.0	36.0	41.0	32.0	41.0
15	37.60498524684018	40.0	36.0	41.0	32.0	41.0
16	37.84067908574448	40.0	36.0	41.0	33.0	41.0
17	37.53302652632815	40.0	36.0	41.0	32.0	41.0
18	37.62122546644941	39.0	36.0	41.0	33.0	41.0
19	37.52940796523833	39.0	36.0	41.0	32.0	41.0
20	37.068064179915154	39.0	35.0	41.0	31.0	41.0
21	37.427752088196	39.0	35.0	41.0	32.0	41.0
22	37.427951732945786	39.0	35.0	41.0	32.0	41.0
23	37.16841943013168	39.0	35.0	41.0	31.0	41.0
24	37.296476857356765	39.0	35.0	41.0	31.0	41.0
25	37.3850927026908	39.0	35.0	41.0	32.0	41.0
26	37.1542975000367	39.0	35.0	41.0	31.0	41.0
27	36.944943556318904	39.0	35.0	41.0	31.0	41.0
28	36.899690257042614	39.0	35.0	41.0	30.0	41.0
29	36.9014165969378	39.0	35.0	41.0	30.0	41.0
30	36.77123647627017	39.0	35.0	41.0	30.0	41.0
31	36.82602134437251	39.0	35.0	41.0	30.0	41.0
32	36.37663862832313	39.0	35.0	41.0	30.0	41.0
33	36.32033734090809	39.0	35.0	41.0	29.0	41.0
34	36.094555276640094	39.0	35.0	41.0	29.0	41.0
35	35.89720350552693	39.0	35.0	41.0	27.0	41.0
36	35.83256411385623	39.0	35.0	41.0	27.0	41.0
37	35.61443020507626	39.0	35.0	41.0	26.0	41.0
38	35.67066396559064	39.0	35.0	41.0	26.0	41.0
39	35.392789301390174	39.0	35.0	41.0	24.0	41.0
40	35.568196297764274	39.0	35.0	41.0	26.0	41.0
41	35.40157367038065	38.0	35.0	41.0	25.0	41.0
42	35.20826617342669	38.0	35.0	41.0	24.0	41.0
43	35.243380161770965	38.0	35.0	40.0	24.0	41.0
44	35.36261505262694	38.0	35.0	40.0	25.0	41.0
45	35.37302447116161	38.0	35.0	40.0	26.0	41.0
46	35.24326272368286	38.0	35.0	40.0	25.0	41.0
47	34.93393520353489	38.0	34.0	40.0	24.0	41.0
48	35.02436106340189	38.0	35.0	40.0	24.0	41.0
49	34.99912215029139	38.0	35.0	40.0	24.0	41.0
50	34.92097297455997	38.0	34.0	40.0	25.0	41.0
51	34.00324716313619	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	15.0
10	28.0
11	19.0
12	9.0
13	15.0
14	13.0
15	25.0
16	44.0
17	103.0
18	238.0
19	672.0
20	1867.0
21	4006.0
22	6650.0
23	8562.0
24	9469.0
25	9175.0
26	8618.0
27	7862.0
28	7768.0
29	8576.0
30	11023.0
31	14963.0
32	20389.0
33	29883.0
34	46042.0
35	53580.0
36	52812.0
37	78378.0
38	119407.0
39	180855.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.378664435343	16.06186051291085	18.475947211579395	10.083527840166761
2	27.813449597040563	36.062888096181794	20.23370179533477	15.889960511442874
3	27.29863037829744	36.72098178241659	22.135024441802088	13.84536339748389
4	24.570983984380735	20.70697729040971	38.191453443137945	16.53058528207161
5	31.517593693574668	32.20328533051482	19.61377548773506	16.665345488175454
6	21.499390789917943	42.17436620131824	23.44754187401829	12.878701134745526
7	69.15562014650402	4.714698844702808	21.918644764463235	4.211036244329942
8	68.16605745658461	16.840915429896803	9.7699681449186	5.223058968599991
9	64.45251831300186	4.846523098603955	9.973282834955446	20.727675753438735
10	45.357819174703835	18.95421382539892	19.06930315174469	16.61866384815255
11	37.16827410049765	20.667195138063153	22.55515920200819	19.609371559431015
12	26.029858633901444	17.715535591080577	28.34617812422014	27.908427650797847
13	29.507787613217655	20.176157132161887	34.01021711366539	16.305838140955064
14	21.047254150702425	30.782431262019056	27.664743617973897	20.50557096930462
15	17.54510356571395	21.10626678997666	37.07476402284171	24.27386562146768
16	21.245137329164283	21.868146386576825	25.79615683856667	31.09055944569222
17	21.163077465098866	26.707329604674037	29.171180693178318	22.95841223704878
18	21.72266995493313	21.51465774137197	28.63096548788186	28.13170681581304
19	20.709179254561736	24.516962463851087	25.447806109716538	29.326052171870643
20	26.75768118495031	25.1213282247765	29.735323908926762	18.38566668134643
21	21.439056972152493	33.27065075380573	27.322558388749428	17.967733885292347
22	19.187328430293153	20.070609650474893	34.225128814902895	26.51693310432906
23	24.36385255648038	27.100160009395047	28.41326463205179	20.122722802072783
24	22.595234949575023	27.723609459638	24.633372968688068	25.047782622098914
25	19.778335608696292	34.65906255046168	23.659370825442963	21.90323101539907
26	18.57298043187857	21.53491581157059	32.26523392199175	27.62686983455909
27	26.996227301419534	23.619735470706537	25.843132073809837	23.54090515406409
28	17.63186095330368	28.41326463205179	29.581626811115513	24.373247603529013
29	21.968702749519238	21.287121445662866	29.905021946242716	26.83915385857518
30	21.00849958162681	28.26103551034189	29.847036890239426	20.883428017791868
31	25.9796538512353	20.724446206015763	23.35476578441303	29.941134158335903
32	28.800076334757268	24.455013872374156	22.572628117614247	24.172281675254325
33	26.085935320972975	20.915283099191146	22.93272265527517	30.066058924560707
34	21.75305706023106	21.622113591990722	27.636264881607726	28.98856446617049
35	23.128844262415406	25.14936656831227	26.53117247251215	25.190616696760177
36	23.564979962126216	29.525990516874383	22.531524786776473	24.377504734222928
37	19.206852512441095	23.28180737217598	30.48399172061479	27.027348394768136
38	23.36885835498598	24.105195167422675	21.845099161785647	30.6808473158057
39	21.19507934410828	23.453707373643955	26.028831050630497	29.322382231617265
40	20.806065677250775	23.071006004022255	26.760470339542874	29.362457979184097
41	18.642562499082516	26.581817648008695	26.002260683196077	28.773359169712716
42	20.258657389057706	25.53059996183262	29.01190528618194	25.198837362927733
43	21.836438102787685	26.017527634650108	24.644235991838055	27.501798270724155
44	22.670982516404635	20.187166952922006	30.273924340511737	26.867926190161622
45	21.075292494238195	19.775840049323996	27.122179650915285	32.02668780552253
46	27.141703733063228	24.690917631860955	24.69796391714743	23.46941471792839
47	17.62261270386518	25.091528309919113	34.314968952305456	22.970890033910248
48	21.92965458522335	26.355896126011068	25.782064267993714	25.932385020771864
49	21.46973767267069	21.225172854185935	33.33259934528266	23.97249012786072
50	22.658504719543167	22.532111977217014	28.047738582815875	26.76164472042395
51	21.02817046138489	21.319416919892547	25.763714566726854	31.88869805199571
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	502.5
2	839.0
3	1990.5
4	3142.0
5	2030.0
6	918.0
7	892.0
8	866.0
9	866.5
10	867.0
11	904.0
12	941.0
13	966.5
14	992.0
15	945.5
16	899.0
17	907.0
18	915.0
19	1026.0
20	1137.0
21	1249.0
22	1361.0
23	1595.0
24	1829.0
25	2015.0
26	2806.0
27	3411.0
28	3985.5
29	4560.0
30	5407.5
31	6255.0
32	7456.0
33	8657.0
34	10053.5
35	11450.0
36	13340.0
37	15230.0
38	15936.0
39	16642.0
40	19102.0
41	21562.0
42	24134.5
43	26707.0
44	34545.5
45	42384.0
46	74777.0
47	107170.0
48	84435.0
49	61700.0
50	63832.0
51	65964.0
52	60273.0
53	54582.0
54	51838.0
55	49094.0
56	45741.5
57	42389.0
58	38599.5
59	34810.0
60	30409.5
61	26009.0
62	22801.0
63	19593.0
64	16794.0
65	13995.0
66	12196.0
67	10397.0
68	8747.0
69	7097.0
70	6221.0
71	5345.0
72	4510.0
73	3675.0
74	3208.5
75	2103.5
76	1465.0
77	1072.0
78	679.0
79	522.0
80	365.0
81	244.0
82	123.0
83	83.0
84	43.0
85	36.0
86	29.0
87	16.5
88	4.0
89	5.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	681210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.016787890865533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03573801161399	22.34317206468191
2	10.505762697778307	6.517100264575141
3	4.096277892876839	3.8116014763620747
4	2.216950135154592	2.7505068842686264
5	1.4166060597321803	2.1969284839813916
6	0.962823840915082	1.7918221709958932
7	0.7590359678806725	1.648000033210485
8	0.6520335436144806	1.6179188896015815
9	0.5569325777191549	1.5546833669365212
>10	6.665249680503016	39.104134956645595
>50	0.07590273757081575	1.6409782022566457
>100	0.04371613366374607	2.4725680820875766
>500	0.004803970732279788	1.02469872848718
>1k	0.006245161951963724	3.7124282129210546
>5k	4.803970732279788E-4	0.7666242486646127
>10k+	0.0014411912196839363	7.046833934323718
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	16145	2.3700474156280733	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	15670	2.300318550813993	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	15478	2.2721334096680907	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	5145	0.7552737041440966	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	3284	0.4820833516830346	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	2787	0.40912493944598577	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	2776	0.40751016573450183	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	2385	0.3501123001717532	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	2305	0.33836849136096064	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	1919	0.28170461384888656	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1870	0.2745115309522761	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	1843	0.2705479954786336	No Hit
GAATATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	1507	0.22122399847330484	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1128	0.1655877042321751	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	1058	0.1553118715227316	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCTGCCTAAT	1043	0.153109907370708	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	1010	0.14826558623625608	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCCATCGAATGC	969	0.1422468842207249	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	825	0.12110802836129828	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGCCCCCCCATCGAATGC	781	0.11464893351536237	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATG	685	0.1005563629424113	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.33988050674535E-4	0.0	0.0	0.12653953993628983	0.0
2	7.33988050674535E-4	0.0	0.0	1.1780508213326286	0.0
3	7.33988050674535E-4	0.0	0.0	1.4359742223396603	0.0
4	7.33988050674535E-4	0.0	0.0	2.140602750987214	0.0
5	7.33988050674535E-4	0.0	0.0	5.261079549624932	0.0
6	7.33988050674535E-4	0.0	0.0	6.032354193273734	0.0
7	7.33988050674535E-4	0.0	0.0	7.114546175188268	0.0
8	7.33988050674535E-4	0.0	0.0	8.32445207792017	0.0
9	7.33988050674535E-4	0.0	0.0	8.673389997210846	0.0
10	7.33988050674535E-4	0.0	0.0	12.038725209553588	0.0
11	7.33988050674535E-4	0.0	0.0	13.1094669778776	0.0
12	7.33988050674535E-4	0.0	0.0	16.674153344783548	0.0
13	7.33988050674535E-4	0.0	0.0	17.139501768911202	0.0
14	7.33988050674535E-4	0.0	0.0	17.399333538849987	0.0
15	7.33988050674535E-4	0.0	0.0	18.057133629864506	0.0
16	7.33988050674535E-4	0.0	0.0	18.69056531759663	0.0
17	7.33988050674535E-4	0.0	0.0	19.353062932135465	0.0
18	7.33988050674535E-4	0.0	0.0	20.060921008205987	0.0
19	7.33988050674535E-4	0.0	0.0	21.00938036728762	0.0
20	7.33988050674535E-4	0.0	0.0	21.509373027407115	0.0
21	7.33988050674535E-4	0.0	0.0	21.981474141600973	0.0
22	7.33988050674535E-4	0.0	0.0	22.6149058293331	0.0
23	7.33988050674535E-4	0.0	0.0	23.12708269109379	0.0
24	7.33988050674535E-4	0.0	0.0	23.53870318991207	0.0
25	7.33988050674535E-4	0.0	0.0	23.91391788141689	0.0
26	7.33988050674535E-4	0.0	0.0	24.266966133791342	0.0
27	7.33988050674535E-4	0.0	0.0	24.795143935056736	0.0
28	7.33988050674535E-4	0.0	0.0	25.1801940664406	0.0
29	7.33988050674535E-4	0.0	0.0	25.577428399465656	0.0
30	7.33988050674535E-4	0.0	0.0	26.107074176832402	0.0
31	7.33988050674535E-4	0.0	0.0	26.496381438910174	0.0
32	7.33988050674535E-4	0.0	0.0	26.868219785381893	0.0
33	7.33988050674535E-4	0.0	0.0	27.279987081810308	0.0
34	7.33988050674535E-4	0.0	0.0	27.70364498465965	0.0
35	7.33988050674535E-4	0.0	0.0	28.15813038563732	0.0
36	7.33988050674535E-4	0.0	0.0	28.530115529719176	0.0
37	8.80785660809442E-4	0.0	0.0	28.928230648405044	0.0
38	8.80785660809442E-4	0.0	0.0	29.39269828687189	0.0
39	8.80785660809442E-4	0.0	0.0	30.085436209098514	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGCT	35	1.2109558E-7	45.000004	28
CGAACCC	110	0.0	45.0	34
GCGACGT	20	7.031261E-4	45.0	17
CCGATCG	25	3.8891274E-5	45.0	40
ACATTCG	20	7.031261E-4	45.0	9
TAGCCGT	25	3.8891274E-5	45.0	44
CCGTCTA	55	1.8189894E-12	45.0	41
ATCGTAA	25	3.8891274E-5	45.0	41
GCGATCG	25	3.8891274E-5	45.0	33
CTCGAAT	20	7.031261E-4	45.0	35
ATACGCC	50	2.1827873E-11	45.0	28
CGATCGC	25	3.8891274E-5	45.0	34
CGATCGA	25	3.8891274E-5	45.0	41
TCGAACG	20	7.031261E-4	45.0	1
TAATACG	710	0.0	44.3662	4
CCGATGA	3455	0.0	44.088276	18
GATGAAT	3720	0.0	43.850807	20
CGATGAA	3535	0.0	43.79066	19
CGTAAGC	130	0.0	43.26923	43
AATACGG	730	0.0	43.150684	5
>>END_MODULE