##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936435.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476926
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.890012287021467	33.0	31.0	34.0	30.0	34.0
2	31.981387049563246	33.0	31.0	34.0	30.0	34.0
3	31.94345034659465	33.0	31.0	34.0	30.0	34.0
4	35.61179512125571	37.0	35.0	37.0	33.0	37.0
5	31.793024494366	37.0	35.0	37.0	0.0	37.0
6	33.66648285058898	37.0	35.0	37.0	17.0	37.0
7	35.39526257742291	37.0	35.0	37.0	32.0	37.0
8	35.661670783308104	37.0	35.0	37.0	33.0	37.0
9	37.59502522403895	39.0	37.0	39.0	35.0	39.0
10	36.997320339004375	39.0	37.0	39.0	33.0	39.0
11	37.080599506003026	39.0	37.0	39.0	34.0	39.0
12	37.10606047898416	39.0	37.0	39.0	34.0	39.0
13	37.051003719654624	39.0	37.0	39.0	33.0	39.0
14	38.2277502170148	40.0	38.0	41.0	33.0	41.0
15	38.32760637918671	40.0	38.0	41.0	34.0	41.0
16	38.320588518973594	40.0	37.0	41.0	34.0	41.0
17	38.306231994061974	40.0	37.0	41.0	34.0	41.0
18	38.252584258354545	40.0	37.0	41.0	34.0	41.0
19	38.113568981351406	40.0	37.0	41.0	34.0	41.0
20	38.140925426586094	40.0	37.0	41.0	34.0	41.0
21	38.13722883633939	40.0	37.0	41.0	34.0	41.0
22	38.22326105098065	40.0	37.0	41.0	34.0	41.0
23	38.22109929003661	40.0	37.0	41.0	34.0	41.0
24	38.1953825121717	40.0	37.0	41.0	34.0	41.0
25	37.96800342191451	40.0	37.0	41.0	34.0	41.0
26	38.01428523502598	40.0	37.0	41.0	34.0	41.0
27	37.98570637792865	40.0	37.0	41.0	34.0	41.0
28	37.910950545786974	40.0	37.0	41.0	33.0	41.0
29	37.873705774061385	40.0	37.0	41.0	33.0	41.0
30	37.71126757610195	40.0	36.0	41.0	33.0	41.0
31	37.734241790130966	40.0	36.0	41.0	33.0	41.0
32	37.62318472886779	40.0	36.0	41.0	33.0	41.0
33	37.56470815178875	40.0	36.0	41.0	33.0	41.0
34	37.515727806829574	40.0	36.0	41.0	33.0	41.0
35	37.52026729513593	40.0	36.0	41.0	33.0	41.0
36	37.446182426623835	40.0	36.0	41.0	33.0	41.0
37	37.371011435736364	40.0	36.0	41.0	33.0	41.0
38	37.283488423780625	40.0	36.0	41.0	32.0	41.0
39	37.21169531541581	40.0	36.0	41.0	32.0	41.0
40	37.172043461669105	40.0	36.0	41.0	32.0	41.0
41	37.10722418152921	40.0	36.0	41.0	31.0	41.0
42	37.11747524773235	40.0	36.0	41.0	32.0	41.0
43	37.041402649467635	40.0	35.0	41.0	32.0	41.0
44	37.000912091183956	40.0	35.0	41.0	31.0	41.0
45	37.00181998884523	40.0	35.0	41.0	31.0	41.0
46	36.87384206354864	39.0	35.0	41.0	31.0	41.0
47	36.765540146689425	39.0	35.0	41.0	31.0	41.0
48	36.74841380004445	39.0	35.0	41.0	31.0	41.0
49	36.7309771327208	39.0	35.0	41.0	31.0	41.0
50	36.622499087908814	39.0	35.0	41.0	31.0	41.0
51	35.57631791934178	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	11.0
10	14.0
11	10.0
12	5.0
13	7.0
14	5.0
15	14.0
16	25.0
17	52.0
18	91.0
19	191.0
20	412.0
21	712.0
22	1188.0
23	1652.0
24	2269.0
25	2944.0
26	3566.0
27	3956.0
28	4290.0
29	5196.0
30	6664.0
31	8616.0
32	11632.0
33	16703.0
34	26920.0
35	36583.0
36	35730.0
37	55722.0
38	95015.0
39	156632.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.72045558430448	18.935222655086953	23.257486486373146	11.086835274235415
2	32.49560728498761	26.707497599208263	23.812289537580252	16.984605578223874
3	30.755924399173036	26.141581712886275	26.672691360923917	16.429802527016772
4	26.641030264653214	24.793783521971964	29.61738299023329	18.947803223141534
5	23.496726955544467	37.34038404280748	22.871263047097454	16.2916259545506
6	22.720925258845188	37.76560724305238	25.33013507336568	14.183332424736752
7	78.57697001211929	5.0561303011368635	11.770379471867754	4.596520214876102
8	79.15022456313977	6.585508024305657	8.765930144299116	5.498337268255453
9	73.88924067884746	5.827738475151281	11.209076460499114	9.073944385502152
10	36.461841040329105	24.410705224709915	22.20239617886213	16.92505755609885
11	28.47255129726624	26.478950612883338	25.356344590146062	19.692153499704357
12	24.031191421729996	22.28920209843875	31.467565198793945	22.21204128103731
13	23.451227234413725	25.65702016665059	33.95054998050012	16.941202618435565
14	21.079370803856364	30.212024506946573	29.329707334051825	19.37889735514524
15	19.234640174786026	26.467628101634215	36.05569836830032	18.242033355279435
16	20.706147284903736	25.763116290577575	31.14466395205965	22.38607247245904
17	20.862775357183295	25.56476266758365	29.873187874009805	23.69927410122325
18	21.767527876442045	25.12045893912263	31.809337297610114	21.30267588682521
19	21.19112818340791	27.91103860976336	28.789162259973246	22.108670946855487
20	21.96860728918113	27.73616871380466	30.614812360827464	19.680411636186747
21	21.412126828900078	29.46683552584678	30.039041696196055	19.081995949057088
22	19.081995949057088	26.156259042283292	29.64506023995337	25.116684768706254
23	18.67648230543103	28.35869715637227	29.74486607985306	23.219954458343643
24	20.415536162842873	26.20008135434009	29.763107903532205	23.621274579284837
25	18.214565781693594	29.802317340635653	28.358487480238026	23.624629397432724
26	18.446048233897923	27.08554366924848	30.75403731396485	23.71437078288875
27	22.29654076313726	27.45331560871079	27.893425814486942	22.356717813665014
28	17.90319672234267	26.294435614749457	31.39438822794312	24.40797943496475
29	20.016732155512596	25.28023215341583	29.12527310316485	25.577762587906722
30	21.61865782112948	28.564808796333185	27.576185823377212	22.24034755916012
31	20.41532648670863	26.88530296104637	25.598520525196783	27.100850027048224
32	20.4448908216369	29.699995387125043	26.32211286446954	23.533000926768512
33	20.54826115581872	29.09843455798174	25.41610228840533	24.937201997794205
34	18.145792009661875	26.855738626118097	26.890754540536687	28.107714823683338
35	19.616670091376857	26.977350783979066	27.565282664396573	25.840696460247504
36	20.732566477818363	28.613243983343327	25.90129286304374	24.752896675794567
37	19.692782528107085	27.66341109522232	28.66566301690409	23.978143359766506
38	17.861471171628303	30.29212079022742	26.85322251250718	24.993185525637102
39	19.8326365096472	29.101370023861143	27.31996158733221	23.746031879159453
40	20.164553830153945	26.058130611457543	29.439787304529425	24.33752825385909
41	18.054163538997663	27.313042274902188	26.994334550852756	27.638459635247397
42	20.36731065196697	25.029878849129634	29.795607704339876	24.80720279456352
43	21.3376918012438	26.54772438491506	27.513073306970053	24.601510506871087
44	19.91378117359926	26.349370761921136	27.43025123394405	26.306596830535554
45	19.730524232270835	25.557004650616655	26.89431903481882	27.81815208229369
46	22.200089741385458	26.91780276185404	27.108608044015213	23.773499452745288
47	18.113292208854205	25.460553628864858	31.442194386550533	24.98395977573041
48	19.327526702255696	25.791632244834627	28.79838800987994	26.082453043029734
49	20.26184355644272	23.625258425835455	31.582677396493374	24.53022062122845
50	20.293924004981907	23.515807483760582	29.96313893560007	26.227129575657436
51	19.466541979258835	23.329405400418512	26.866851461232983	30.337201159089673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	238.0
2	374.0
3	1163.5
4	1953.0
5	1397.0
6	841.0
7	852.5
8	864.0
9	920.0
10	976.0
11	941.0
12	906.0
13	928.0
14	950.0
15	972.0
16	994.0
17	1009.0
18	1024.0
19	1001.5
20	979.0
21	1014.0
22	1049.0
23	1351.5
24	1654.0
25	1866.0
26	2675.5
27	3273.0
28	3972.0
29	4671.0
30	5235.0
31	5799.0
32	7606.0
33	9413.0
34	10617.5
35	11822.0
36	13198.5
37	14575.0
38	16247.0
39	17919.0
40	19972.0
41	22025.0
42	26133.0
43	30241.0
44	34945.5
45	39650.0
46	50630.5
47	61611.0
48	60317.0
49	59023.0
50	58205.5
51	57388.0
52	48692.5
53	39997.0
54	33938.5
55	27880.0
56	23459.0
57	19038.0
58	16077.5
59	13117.0
60	11463.5
61	9810.0
62	8138.5
63	6467.0
64	5029.5
65	3592.0
66	2886.0
67	2180.0
68	1552.5
69	925.0
70	763.5
71	602.0
72	523.0
73	444.0
74	403.0
75	268.0
76	174.0
77	117.5
78	61.0
79	63.5
80	66.0
81	41.5
82	17.0
83	24.0
84	31.0
85	18.5
86	6.0
87	4.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	476926.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.54162775970296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.89264116369752	28.822936833165286
2	11.288059262981582	8.926964750125695
3	4.238406447701344	5.027804701479945
4	2.243101016990013	3.54783461764921
5	1.4078744914905805	2.783482453745082
6	0.9641608377799787	2.287469336878756
7	0.8111066971938167	2.2450735365688006
8	0.6089973595798704	1.926459751931937
9	0.5411409916625641	1.9257836092053873
>10	4.9027597478334215	34.08762320071067
>50	0.06406606342660141	1.7511539436970889
>100	0.030148735730164352	2.1994800080571197
>500	0.004306962247166336	1.1714806895410694
>1k	0.0032302216853747527	3.296452567243951
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTC	3631	0.761334043436508	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGC	3561	0.7466567140394946	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCG	3149	0.6602701467313588	No Hit
GCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	2741	0.5747222839601951	No Hit
CCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	1327	0.27824023014052496	No Hit
CTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGCT	1076	0.22561152044551983	TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	993	0.20820840130334686	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCT	755	0.1583054813535014	No Hit
GAACTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCT	716	0.15012811211802252	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTTCTGC	681	0.14278944741951582	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGCAACTAT	636	0.13335402137857863	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTCTT	611	0.12811211802250244	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGCAACTATCGTATGCCGTC	577	0.1209831294582388	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGCAACTATCGTA	534	0.11196705568578774	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0967613424304819E-4	0.0	0.0	0.23336953741251262	0.0
2	2.0967613424304819E-4	0.0	0.0	1.3746367360974239	0.0
3	2.0967613424304819E-4	0.0	0.0	1.8225049588405748	0.0
4	2.0967613424304819E-4	0.0	0.0	2.52240389494387	0.0
5	2.0967613424304819E-4	0.0	0.0	4.6854228957951545	0.0
6	2.0967613424304819E-4	0.0	0.0	5.848706088575586	0.0
7	2.0967613424304819E-4	0.0	0.0	6.9610379807349565	0.0
8	2.0967613424304819E-4	0.0	0.0	8.737204513907818	0.0
9	2.0967613424304819E-4	0.0	0.0	9.331636354486859	0.0
10	2.0967613424304819E-4	0.0	0.0	11.518348758507608	0.0
11	2.0967613424304819E-4	0.0	0.0	13.364337444383406	0.0
12	2.0967613424304819E-4	0.0	0.0	15.641210586128667	0.0
13	2.0967613424304819E-4	0.0	0.0	16.30609360781337	0.0
14	2.0967613424304819E-4	0.0	0.0	16.593349911726346	0.0
15	2.0967613424304819E-4	0.0	0.0	17.155072275363473	0.0
16	2.0967613424304819E-4	0.0	0.0	18.177662782066818	0.0
17	2.0967613424304819E-4	0.0	0.0	19.39168759933407	0.0
18	2.0967613424304819E-4	0.0	0.0	20.760453403672688	0.0
19	2.0967613424304819E-4	0.0	0.0	21.658076934367177	0.0
20	2.0967613424304819E-4	0.0	0.0	22.434507659469183	0.0
21	2.0967613424304819E-4	0.0	0.0	23.32584090613638	0.0
22	2.0967613424304819E-4	0.0	0.0	24.25659326604127	0.0
23	2.0967613424304819E-4	0.0	0.0	25.30958681220986	0.0
24	2.0967613424304819E-4	0.0	0.0	26.05519514557814	0.0
25	2.0967613424304819E-4	0.0	0.0	26.748174769251413	0.0
26	2.0967613424304819E-4	0.0	0.0	27.388525683229684	0.0
27	2.0967613424304819E-4	0.0	0.0	28.037263642577674	0.0
28	2.0967613424304819E-4	0.0	0.0	28.679291965629886	0.0
29	2.0967613424304819E-4	0.0	0.0	29.35423944175826	0.0
30	2.0967613424304819E-4	0.0	0.0	30.10886384889899	0.0
31	6.290284027291445E-4	0.0	0.0	30.767246910422163	0.0
32	6.290284027291445E-4	0.0	0.0	31.434855721852028	0.0
33	6.290284027291445E-4	0.0	0.0	32.06807764726603	0.0
34	8.387045369721927E-4	0.0	0.0	32.702976981753984	0.0
35	8.387045369721927E-4	0.0	0.0	33.34186016279255	0.0
36	8.387045369721927E-4	0.0	0.0	33.974872412072315	0.0
37	8.387045369721927E-4	0.0	0.0	34.603900814801456	0.0
38	8.387045369721927E-4	0.0	0.0	35.25054201280702	0.0
39	8.387045369721927E-4	0.0	0.0	35.87852203486495	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	30	2.1628202E-6	45.000004	9
CGCGACC	30	2.1628202E-6	45.000004	10
CCATCGA	30	2.1628202E-6	45.000004	41
CATCGAA	30	2.1628202E-6	45.000004	42
GGCGCGA	30	2.1628202E-6	45.000004	8
TGTAACG	30	2.1628202E-6	45.000004	1
CCACCCG	30	2.1628202E-6	45.000004	37
CGGAAGG	35	1.2099372E-7	45.0	2
CGAACGG	20	7.0291315E-4	45.0	2
CGCATTC	20	7.0291315E-4	45.0	12
CGCATGG	20	7.0291315E-4	45.0	2
AGGCGAC	20	7.0291315E-4	45.0	17
ACGGGTT	55	1.8189894E-12	45.0	5
TACGCAC	25	3.8873615E-5	45.0	33
GTTGTCG	20	7.0291315E-4	45.0	37
CGCTAGG	20	7.0291315E-4	45.0	2
CGCTAAT	20	7.0291315E-4	45.0	28
ACTCGTA	20	7.0291315E-4	45.0	10
CGAGCCC	25	3.8873615E-5	45.0	34
GATGACG	20	7.0291315E-4	45.0	1
>>END_MODULE