##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936434.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219825
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.940984874331853	33.0	31.0	34.0	30.0	34.0
2	32.04824292050495	33.0	31.0	34.0	30.0	34.0
3	32.022072102809055	33.0	31.0	34.0	30.0	34.0
4	35.67127942681679	37.0	35.0	37.0	33.0	37.0
5	32.09927897190947	37.0	35.0	37.0	0.0	37.0
6	33.84046400545889	37.0	35.0	37.0	17.0	37.0
7	35.44938473785966	37.0	35.0	37.0	32.0	37.0
8	35.64126919140225	37.0	35.0	37.0	33.0	37.0
9	37.55041510292278	39.0	37.0	39.0	35.0	39.0
10	37.27973615375867	39.0	37.0	39.0	34.0	39.0
11	37.25491186170818	39.0	37.0	39.0	34.0	39.0
12	37.05343341294211	39.0	35.0	39.0	34.0	39.0
13	36.87693392471284	39.0	35.0	39.0	33.0	39.0
14	38.00094165813715	40.0	37.0	41.0	33.0	41.0
15	38.13856021835551	40.0	37.0	41.0	33.0	41.0
16	38.16059592857955	40.0	37.0	41.0	34.0	41.0
17	38.127123848515865	40.0	36.0	41.0	34.0	41.0
18	38.08216535880814	40.0	36.0	41.0	34.0	41.0
19	37.976363016035485	40.0	36.0	41.0	34.0	41.0
20	37.99564198794496	40.0	36.0	41.0	34.0	41.0
21	37.97387012396224	40.0	36.0	41.0	34.0	41.0
22	38.03890822244968	40.0	36.0	41.0	34.0	41.0
23	38.03737063573297	40.0	36.0	41.0	34.0	41.0
24	37.978887751620604	40.0	36.0	41.0	34.0	41.0
25	37.76453542590697	40.0	35.0	41.0	33.0	41.0
26	37.77257818719436	40.0	35.0	41.0	33.0	41.0
27	37.7269054930058	40.0	35.0	41.0	33.0	41.0
28	37.67618332764699	40.0	36.0	41.0	33.0	41.0
29	37.660652791993634	40.0	36.0	41.0	33.0	41.0
30	37.523898555669284	40.0	36.0	41.0	33.0	41.0
31	37.52624587740248	40.0	35.0	41.0	33.0	41.0
32	37.41436142385989	40.0	35.0	41.0	33.0	41.0
33	37.33402934152166	40.0	35.0	41.0	33.0	41.0
34	37.27493688161037	40.0	35.0	41.0	33.0	41.0
35	37.286382349596266	40.0	35.0	41.0	33.0	41.0
36	37.18455134766291	40.0	35.0	41.0	33.0	41.0
37	37.12146025247356	40.0	35.0	41.0	32.0	41.0
38	36.98656203798476	39.0	35.0	41.0	32.0	41.0
39	36.940670988286136	39.0	35.0	41.0	32.0	41.0
40	36.85202774934607	39.0	35.0	41.0	31.0	41.0
41	36.76232457636757	39.0	35.0	41.0	31.0	41.0
42	36.817204594563854	39.0	35.0	41.0	32.0	41.0
43	36.73577163652906	39.0	35.0	41.0	32.0	41.0
44	36.678744455817125	39.0	35.0	41.0	31.0	41.0
45	36.70105765950188	39.0	35.0	41.0	32.0	41.0
46	36.56679631525077	39.0	35.0	41.0	31.0	41.0
47	36.43373137723189	39.0	35.0	41.0	31.0	41.0
48	36.44525417946093	39.0	35.0	41.0	31.0	41.0
49	36.456494939156144	39.0	35.0	41.0	31.0	41.0
50	36.34684408051859	38.0	35.0	41.0	31.0	41.0
51	35.2891663823496	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	10.0
10	11.0
11	6.0
12	4.0
13	5.0
14	3.0
15	7.0
16	11.0
17	36.0
18	38.0
19	111.0
20	182.0
21	308.0
22	557.0
23	745.0
24	1108.0
25	1264.0
26	1565.0
27	1826.0
28	2069.0
29	2362.0
30	3157.0
31	4110.0
32	5597.0
33	8706.0
34	15035.0
35	21330.0
36	16600.0
37	24588.0
38	40634.0
39	67784.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.83248038212214	20.089616740589104	23.58284999431366	11.495052882975095
2	32.40532241555783	26.65711361310133	24.038212214261346	16.899351757079494
3	30.860002274536562	26.884112362106222	25.529853292391675	16.72603207096554
4	28.125554418287273	23.520527692482656	30.169452973956556	18.184464915273512
5	23.85306493801888	35.62424655976345	21.779142499715682	18.74354600250199
6	24.061412487205732	36.79472307517344	24.53952007278517	14.604344364835665
7	75.8689866939611	5.643580120550438	13.361537586716707	5.12589559877175
8	75.02740816558627	8.276583646082111	10.240418514727624	6.4555896736040035
9	69.2509951097464	6.639827135221199	12.759695212100535	11.349482542931879
10	40.872512225634026	23.25941089503014	19.806664392130102	16.061412487205732
11	32.87069259638349	22.0129648584101	25.137723188900264	19.97861935630615
12	28.2388263391334	19.63880359376777	30.583873535767086	21.53849653133174
13	24.038212214261346	25.50710792675992	32.522688502217676	17.93199135676106
14	18.288183782554306	31.43818946889571	29.239167519617876	21.034459228932107
15	16.775616968042762	23.458205390651653	39.62697600363926	20.139201637666325
16	18.989650858637553	22.81451154327306	32.9357443420903	25.260093255999088
17	18.58705788695553	22.949164107813033	29.947458205390653	28.516319799840783
18	20.003184351188448	23.34083930399181	32.5477084044126	24.108267940407142
19	21.671329466621174	26.245422495166608	27.828499943136585	24.25474809507563
20	23.619242579324464	24.721028090526556	29.89332423518708	21.7664050949619
21	21.16092346184465	28.391220288866144	29.329239167519617	21.11861708176959
22	19.33583532355283	24.31889002615717	28.753326509723642	27.59194814056636
23	19.268963948595474	27.74570681223701	28.538610258159903	24.44671898100762
24	22.33822358694416	24.111907198908224	29.65267826680314	23.89719094734448
25	19.445922893210508	27.539633799613327	28.237461617195496	24.77698168998067
26	18.965540771067896	24.562265438416922	30.684408051859435	25.78778573865575
27	22.61844649152735	25.686341407938134	28.575002843170704	23.120209257363815
28	18.3491413624474	24.73513021721824	33.31468213351529	23.60104628681906
29	19.689753212782897	22.243147958603434	32.97304674172638	25.0940520868873
30	22.837711816217446	25.754122597520755	30.34550210394632	21.06266348231548
31	26.11213465256454	23.83441373820084	26.05800068236097	23.995450926873648
32	24.128738769475717	26.046173092232454	25.31058796770158	24.514500170590242
33	23.705220061412486	26.073012623677926	25.30012509951097	24.921642215398613
34	21.402934152166498	22.825884226088935	30.867735698851355	24.903445922893212
35	20.890708518139427	23.314909587171613	27.27305811440919	28.52132378027977
36	24.857500284317073	26.63573296940748	27.9558739906744	20.550892755601048
37	22.786307289889685	24.772432616854314	29.5808029114068	22.8604571818492
38	20.797452519049244	28.115091550096665	24.275218924144205	26.812237006709882
39	21.49164107813033	25.11588763789378	28.489025361082682	24.903445922893212
40	20.840668713749576	23.656999886273173	30.406914591152052	25.0954168088252
41	18.952348459001477	24.563175253042193	29.270556124189696	27.21392016376663
42	21.40338905947913	22.953713180939385	29.474354600250198	26.168543159331286
43	23.32992152848857	24.34754918685318	27.11293074036165	25.2095985442966
44	21.85556692823837	23.41999317639031	28.40805185943364	26.316388035937678
45	20.468554532014103	23.57466166268623	27.162515637438872	28.7942681678608
46	24.809280109177756	24.640054588877515	27.677015808029115	22.873649493915615
47	19.196178778573866	23.374502445126804	33.39338109860116	24.03593767769817
48	20.096895257591267	23.595132491754807	28.825656772432616	27.482315478221313
49	20.66279995450927	21.336062777209143	33.130899579210734	24.87023768907085
50	20.9357443420903	21.510292277948366	31.253952007278517	26.300011372682814
51	19.79483680200159	22.06436938473786	27.59285795519163	30.547935858068918
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	246.0
2	362.0
3	714.5
4	1067.0
5	725.0
6	383.0
7	397.5
8	412.0
9	411.0
10	410.0
11	422.0
12	434.0
13	431.0
14	428.0
15	449.0
16	470.0
17	479.0
18	488.0
19	432.0
20	376.0
21	486.0
22	596.0
23	618.0
24	640.0
25	842.5
26	1222.5
27	1400.0
28	1663.5
29	1927.0
30	2404.5
31	2882.0
32	3084.0
33	3286.0
34	4011.5
35	4737.0
36	4879.5
37	5022.0
38	5856.0
39	6690.0
40	7604.5
41	8519.0
42	9681.5
43	10844.0
44	13323.5
45	15803.0
46	22211.0
47	28619.0
48	26822.0
49	25025.0
50	24902.5
51	24780.0
52	21379.0
53	17978.0
54	15720.0
55	13462.0
56	11889.5
57	10317.0
58	9173.5
59	8030.0
60	7348.0
61	6666.0
62	5870.5
63	5075.0
64	4522.0
65	3969.0
66	3381.5
67	2794.0
68	2250.0
69	1706.0
70	1551.0
71	1396.0
72	1028.5
73	661.0
74	594.5
75	358.5
76	189.0
77	173.5
78	158.0
79	109.0
80	60.0
81	54.5
82	49.0
83	29.0
84	9.0
85	5.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	219825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.46718981007619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.27852901551819	26.722620266120778
2	11.067312010378725	8.071875355396338
3	3.9119804400977993	4.279767997270556
4	1.9846814031235966	2.8950301376094623
5	1.268649268998553	2.3132036847492325
6	0.8682201486951748	1.8996929375639713
7	0.6324534703857093	1.6144660525417946
8	0.5451324784192406	1.5903559649721368
9	0.4553166009680156	1.4943705220061412
>10	5.872960431116211	40.096895257591264
>50	0.069856793573175	1.887410440122825
>100	0.03617583952896562	2.6762197202320026
>500	0.0037423282271343747	0.9516660980325258
>1k	0.004989770969512499	3.50642556579097
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGC	2229	1.0139883998635277	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTC	2166	0.9853292391675196	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCG	1911	0.8693278744455817	No Hit
GCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	1402	0.6377800523143409	No Hit
CCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	854	0.3884908449903332	No Hit
CTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCT	639	0.2906857727737973	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.2724894802683953	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCT	453	0.20607301262367791	No Hit
GAACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCT	437	0.1987944956215171	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGCATCAT	430	0.19561014443307176	No Hit
TCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	361	0.1642215398612533	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTC	338	0.1537586716706471	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGCATCATCGTA	325	0.14784487660639145	No Hit
ACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	273	0.12418969634936881	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGC	236	0.10735812578187194	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	220	0.10007960877971113	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30069373365176844	0.0
2	0.0	0.0	0.0	1.6640509496190152	0.0
3	0.0	0.0	0.0	2.1198680768793356	0.0
4	0.0	0.0	0.0	2.900489025361083	0.0
5	0.0	0.0	0.0	5.43295803480041	0.0
6	0.0	0.0	0.0	6.445581712726032	0.0
7	0.0	0.0	0.0	7.664278403275333	0.0
8	0.0	0.0	0.0	9.596269760036392	0.0
9	0.0	0.0	0.0	10.29045831911748	0.0
10	0.0	0.0	0.0	12.599567838052996	0.0
11	0.0	0.0	0.0	14.380075059706584	0.0
12	0.0	0.0	0.0	16.999886273171843	0.0
13	0.0	0.0	0.0	17.665870578869555	0.0
14	0.0	0.0	0.0	17.93563061526214	0.0
15	0.0	0.0	0.0	18.4906175366769	0.0
16	0.0	0.0	0.0	19.45047196633686	0.0
17	0.0	0.0	0.0	20.55862617991584	0.0
18	0.0	0.0	0.0	21.859661094052086	0.0
19	0.0	0.0	0.0	22.749459797566246	0.0
20	0.0	0.0	0.0	23.41999317639031	0.0
21	0.0	0.0	0.0	24.215626066189014	0.0
22	0.0	0.0	0.0	25.029910155805755	0.0
23	0.0	0.0	0.0	25.804617309223246	0.0
24	0.0	0.0	0.0	26.392357557147733	0.0
25	0.0	0.0	0.0	26.934152166496077	0.0
26	0.0	0.0	0.0	27.450926873649493	0.0
27	0.0	0.0	0.0	27.979074263618788	0.0
28	0.0	0.0	0.0	28.4944842488343	0.0
29	0.0	0.0	0.0	29.017627658364606	0.0
30	0.0	0.0	0.0	29.644944842488343	0.0
31	0.0	0.0	0.0	30.169452973956556	0.0
32	0.0	0.0	0.0	30.69941999317639	0.0
33	0.0	0.0	0.0	31.19936312976231	0.0
34	0.0	0.0	0.0	31.750710792675992	0.0
35	0.0	0.0	0.0	32.38985556692824	0.0
36	0.0	0.0	0.0	32.87205731832139	0.0
37	0.0	0.0	0.0	33.35334925508928	0.0
38	0.0	0.0	0.0	33.875582849994316	0.0
39	0.0	0.0	0.0	34.39099283520983	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAG	35	1.2059354E-7	45.000004	1
CGTTAGG	35	1.2059354E-7	45.000004	2
ACGTAAG	35	1.2059354E-7	45.000004	1
GTTTGAT	20	7.020828E-4	45.0	29
AGGTACG	20	7.020828E-4	45.0	1
GTACCGG	20	7.020828E-4	45.0	2
ACCGGTG	20	7.020828E-4	45.0	39
GGTACCG	20	7.020828E-4	45.0	1
TCGCAAT	20	7.020828E-4	45.0	28
GGTAAAT	20	7.020828E-4	45.0	7
CCGATTA	20	7.020828E-4	45.0	18
CACATGG	25	3.880482E-5	45.0	22
CGGTTCG	20	7.020828E-4	45.0	29
AGCCACT	25	3.880482E-5	45.0	14
CACGCCC	20	7.020828E-4	45.0	27
TTACTCA	20	7.020828E-4	45.0	27
AATGGGC	40	6.7720975E-9	45.0	4
CGATTAA	20	7.020828E-4	45.0	19
TCGGCGG	20	7.020828E-4	45.0	33
TATTGCC	20	7.020828E-4	45.0	25
>>END_MODULE