FastQCFastQC Report
Sat 14 Jan 2017
SRR2936433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499644
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC58491.1706334910456246No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC54911.098982475522572No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG51861.0379390125769548No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC29570.5918213768202961No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTT11730.23476715421380023No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCT11130.22275860412613782No Hit
CCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC10550.2111503390413975No Hit
GAACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT10020.20054278646396234No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.19333765641136488No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCCAACTT9290.1859323838573064No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTC8800.1761254012857154No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTA8300.16611827621266342No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT8280.16571799120974134No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC6940.13889889601396194No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG6520.13049291095259824No Hit
GCGCAAGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC5770.11548222334302023No Hit
GAATATGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC5420.10847723579188381No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC5400.10807695078896173No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC253.8876286E-545.025
ATCTCGA207.0294546E-445.042
GTCGATG207.0294546E-445.01
CGATTGT207.0294546E-445.010
TACGGGT406.8012014E-945.04
GCGCAAC253.8876286E-545.024
GTTACGG351.2100827E-745.02
GTACGAG253.8876286E-545.01
CCCCTCG302.1630294E-644.99999640
TAATACG2700.043.3333324
TCGAATG900.042.50000444
CCGATGA6900.042.391318
CGATGAA7050.041.80851419
CGAACAC700.041.78571334
ATAATAC3500.041.7857133
CGTTATT700.041.7857131
AACGGGA2200.040.9090924
ACGGGAT1050.040.7142875
ACAACGG501.0786607E-940.52
TTACGGG1000.040.53
Produced by FastQC (version 0.11.5)