##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936433.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499644
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.937343388492607	33.0	31.0	34.0	30.0	34.0
2	32.02465155190496	33.0	31.0	34.0	30.0	34.0
3	32.0140139779523	33.0	31.0	34.0	30.0	34.0
4	35.66018805389437	37.0	35.0	37.0	33.0	37.0
5	31.889791531570477	37.0	35.0	37.0	0.0	37.0
6	33.71374818871036	37.0	35.0	37.0	17.0	37.0
7	35.36098902418522	37.0	35.0	37.0	32.0	37.0
8	35.57228947010271	37.0	35.0	37.0	33.0	37.0
9	37.48273770924899	39.0	37.0	39.0	35.0	39.0
10	37.18072067311926	39.0	37.0	39.0	34.0	39.0
11	37.17792868522388	39.0	37.0	39.0	34.0	39.0
12	37.03345381911921	39.0	37.0	39.0	33.0	39.0
13	36.88635108197036	39.0	37.0	39.0	33.0	39.0
14	38.045470374906934	40.0	37.0	41.0	33.0	41.0
15	38.12713852262811	40.0	37.0	41.0	33.0	41.0
16	38.16008598121863	40.0	37.0	41.0	34.0	41.0
17	38.068206563072906	40.0	37.0	41.0	33.0	41.0
18	38.0308759837004	40.0	37.0	41.0	33.0	41.0
19	37.90430386435142	40.0	37.0	41.0	33.0	41.0
20	37.898095443956095	40.0	36.0	41.0	33.0	41.0
21	37.88268046849357	40.0	36.0	41.0	33.0	41.0
22	37.97410156031094	40.0	36.0	41.0	33.0	41.0
23	37.923771725468534	40.0	36.0	41.0	33.0	41.0
24	37.939154678130826	40.0	36.0	41.0	33.0	41.0
25	37.73663848660246	40.0	36.0	41.0	33.0	41.0
26	37.720551032335024	40.0	36.0	41.0	33.0	41.0
27	37.6403699433997	40.0	36.0	41.0	33.0	41.0
28	37.516932055623606	40.0	36.0	41.0	33.0	41.0
29	37.45427944696624	40.0	36.0	41.0	33.0	41.0
30	37.2969314151676	40.0	35.0	41.0	32.0	41.0
31	37.340194218283415	40.0	35.0	41.0	32.0	41.0
32	37.198633427000026	40.0	35.0	41.0	32.0	41.0
33	37.07532763327489	39.0	35.0	41.0	32.0	41.0
34	36.94961012240715	39.0	35.0	41.0	31.0	41.0
35	36.94428833329331	39.0	35.0	41.0	31.0	41.0
36	36.84713516023409	39.0	35.0	41.0	31.0	41.0
37	36.76902954903892	39.0	35.0	41.0	31.0	41.0
38	36.62647805237329	39.0	35.0	41.0	31.0	41.0
39	36.52593846818935	39.0	35.0	41.0	31.0	41.0
40	36.40371144254709	39.0	35.0	41.0	30.0	41.0
41	36.2830275155911	39.0	35.0	41.0	30.0	41.0
42	36.34332044415624	39.0	35.0	41.0	30.0	41.0
43	36.200602829214404	39.0	35.0	41.0	30.0	41.0
44	36.19192465035105	39.0	35.0	41.0	30.0	41.0
45	36.127831015683164	38.0	35.0	40.0	30.0	41.0
46	35.97436574841287	38.0	35.0	40.0	30.0	41.0
47	35.82135280319588	38.0	35.0	40.0	30.0	41.0
48	35.82920239210318	38.0	35.0	40.0	30.0	41.0
49	35.80631009278606	38.0	35.0	40.0	30.0	41.0
50	35.62280343604647	38.0	35.0	40.0	29.0	41.0
51	34.63810833313319	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	19.0
9	24.0
10	18.0
11	19.0
12	8.0
13	10.0
14	11.0
15	22.0
16	31.0
17	63.0
18	140.0
19	320.0
20	710.0
21	1288.0
22	1877.0
23	2453.0
24	3101.0
25	3649.0
26	4132.0
27	4719.0
28	5269.0
29	6213.0
30	7994.0
31	10357.0
32	14046.0
33	20334.0
34	32710.0
35	42496.0
36	41574.0
37	62654.0
38	95109.0
39	138174.0
40	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.14370231604902	17.98540560879346	21.00335438832449	10.867537686833025
2	30.48110254501205	28.827325055439474	22.247640319907774	18.443932079640703
3	31.815652744754264	29.267238273650843	23.33921752287629	15.577891458718607
4	26.60494271921608	24.309908654962335	29.081305889793533	20.00384273602805
5	25.950276596937016	35.13561655899	20.379710353771884	18.534396490301095
6	23.253356389749502	37.70404528023953	23.13767402390502	15.904924306105947
7	76.39139067015715	5.336199373954256	13.63290662952022	4.6395033263683745
8	75.55559558405585	9.607840782637238	9.184539392047137	5.6520242412597765
9	70.36950308619737	6.3859467941174115	10.868938684343252	12.375611435341964
10	39.93043046649215	26.794677810601147	17.53328369799297	15.74160802491374
11	33.35895157352034	23.980874382560383	23.038203200678883	19.621970843240387
12	26.3433564698065	21.78731256654738	28.380606992178432	23.488723971467685
13	25.21955632410276	25.45432347831656	31.349921143854427	17.97619905372625
14	21.030373626021728	30.04679331684159	27.209573216129883	21.713259841006796
15	18.227177750558397	23.52454947922921	35.145623684063054	23.102649086149338
16	21.289958450416695	25.104274243261205	27.880651023528753	25.725116282793348
17	21.027971916004194	25.647260849725008	27.688714364627614	25.636052869643184
18	25.412493695511202	21.595375907646243	28.627582838981354	24.364547557861197
19	23.29518617255486	24.965975774751623	25.40648942046737	26.332348632226143
20	27.377092489852778	24.438800425903242	27.231789033792058	20.95231805045192
21	23.548966864407458	29.8874798856786	26.859924266077446	19.70362898383649
22	23.242148409667685	21.618592437815725	28.120421740279077	27.018837412237513
23	21.61779186780988	28.169856938139954	27.04245422740992	23.169896966640245
24	22.06991377861037	24.34953687025162	28.361393312038167	25.21915603909984
25	21.477692116787154	29.74217642961789	25.341643249993993	23.43848820360096
26	22.136761374098356	23.54236216185924	27.785983620337678	26.534892843704718
27	24.798656643530194	24.40537662815925	25.847603493687505	24.948363234623052
28	18.984717118588435	26.525686288637512	29.07670261225993	25.412893980514127
29	24.120173563577264	23.431283073548368	27.388100327433136	25.06044303544123
30	22.75480141861005	25.430506520642698	30.098830367221463	21.715861693525788
31	25.054839045400325	24.513453578948212	23.513541641648857	26.918165734002606
32	24.92414599194627	25.513365516247568	23.993483360152428	25.56900513165374
33	21.88498210726037	24.77644082586802	25.129692340946754	28.20888472592486
34	21.820736364291378	26.182441898631826	27.309044039356024	24.68777769772078
35	20.42834498162692	25.19593950893036	27.50118084075862	26.874534668684102
36	23.605807334822394	28.01854920703541	25.261185964406657	23.11445749373554
37	19.802699522059704	25.19834121894789	28.177462353195477	26.821496905796927
38	19.342772053702237	28.345582054422746	23.88240427184155	28.429241620033462
39	22.567868322245438	25.124888920911687	25.357254365107956	26.949988391734912
40	24.047321693045447	23.936843032238954	26.984412901986214	25.031422372729384
41	19.675609033631947	25.539584183938963	26.82289790330715	27.961908879121932
42	22.50842599931151	22.43177142125193	28.248913226217066	26.81088935321949
43	23.48592197644723	22.445981538855666	27.70512604974742	26.362970434949684
44	22.740591301006315	22.92592325735924	27.798192312926805	26.535293128707636
45	22.458990801450632	21.82013593678699	26.08116979289254	29.63970346886984
46	24.753024153197074	24.559886639287175	25.24717598930438	25.43991321821137
47	19.40581694166246	25.51756850877825	31.037298556572278	24.039315992987007
48	21.603581750206146	25.326832704885877	27.14752904067696	25.922056504231012
49	23.37063989560567	21.70405328593959	30.734082666858804	24.191224151595936
50	21.756690763823844	23.546765296891387	29.655514726485254	25.041029212799515
51	22.122150971491703	21.183282497137963	26.286115714388647	30.40845081698169
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	181.0
1	479.5
2	778.0
3	1331.5
4	1885.0
5	1284.0
6	683.0
7	653.5
8	624.0
9	651.0
10	678.0
11	717.0
12	756.0
13	769.0
14	782.0
15	745.5
16	709.0
17	772.0
18	835.0
19	811.0
20	787.0
21	925.0
22	1063.0
23	1169.5
24	1276.0
25	1524.0
26	2222.5
27	2673.0
28	3218.5
29	3764.0
30	4487.5
31	5211.0
32	6296.5
33	7382.0
34	8472.0
35	9562.0
36	10401.5
37	11241.0
38	12526.0
39	13811.0
40	15073.5
41	16336.0
42	18950.5
43	21565.0
44	26247.0
45	30929.0
46	45224.0
47	59519.0
48	54396.5
49	49274.0
50	50690.5
51	52107.0
52	47036.5
53	41966.0
54	38316.5
55	34667.0
56	31014.0
57	27361.0
58	25195.0
59	23029.0
60	21556.0
61	20083.0
62	17614.0
63	15145.0
64	14122.0
65	13099.0
66	10931.0
67	8763.0
68	7509.0
69	6255.0
70	5653.0
71	5051.0
72	3950.5
73	2850.0
74	2614.0
75	1951.0
76	1524.0
77	1150.5
78	777.0
79	576.5
80	376.0
81	235.5
82	95.0
83	60.0
84	25.0
85	17.5
86	10.0
87	7.5
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	499644.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.89094744483695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.06491317725484	24.762391325519182
2	10.377894431021298	7.034333494996177
3	3.891179346376445	3.956272641794372
4	2.0763784728237082	2.814817347922364
5	1.2846289479117046	2.176864607989588
6	1.0008987554903426	2.035284427195554
7	0.7206981978048507	1.7097601321775027
8	0.5916040134213341	1.6040016421613634
9	0.5104807762997009	1.5570609445055454
>10	6.342851155884198	41.31468708904822
>50	0.07547655006595562	1.7654033664094075
>100	0.052298711856725154	2.972081240700196
>500	0.005943035438264222	1.498125526452159
>1k	0.002971517719132111	1.4703302424174187
>5k	0.0017829106314792668	3.3285859707109946
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC	5849	1.1706334910456246	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	5491	1.098982475522572	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	5186	1.0379390125769548	No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	2957	0.5918213768202961	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTT	1173	0.23476715421380023	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCT	1113	0.22275860412613782	No Hit
CCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	1055	0.2111503390413975	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT	1002	0.20054278646396234	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	966	0.19333765641136488	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCCAACTT	929	0.1859323838573064	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTC	880	0.1761254012857154	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCCAACTTCGTA	830	0.16611827621266342	No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT	828	0.16571799120974134	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	694	0.13889889601396194	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	652	0.13049291095259824	No Hit
GCGCAAGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	577	0.11548222334302023	No Hit
GAATATGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC	542	0.10847723579188381	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	540	0.10807695078896173	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.004275043831208E-4	0.0	0.0	0.17292312126233877	0.0
2	6.004275043831208E-4	0.0	0.0	1.1810409011215985	0.0
3	6.004275043831208E-4	0.0	0.0	1.4920623483920552	0.0
4	6.004275043831208E-4	0.0	0.0	2.0674720400925457	0.0
5	6.004275043831208E-4	0.0	0.0	4.376516079448567	0.0
6	6.004275043831208E-4	0.0	0.0	5.411252812002146	0.0
7	6.004275043831208E-4	0.0	0.0	6.364731688962541	0.0
8	6.004275043831208E-4	0.0	0.0	7.786544019341771	0.0
9	6.004275043831208E-4	0.0	0.0	8.266485737845345	0.0
10	6.004275043831208E-4	0.0	0.0	10.458246271345198	0.0
11	6.004275043831208E-4	0.0	0.0	11.722146168071667	0.0
12	6.004275043831208E-4	0.0	0.0	14.06221229515415	0.0
13	6.004275043831208E-4	0.0	0.0	14.603597761606263	0.0
14	6.004275043831208E-4	0.0	0.0	14.860380590980778	0.0
15	6.004275043831208E-4	0.0	0.0	15.432387860156432	0.0
16	6.004275043831208E-4	0.0	0.0	16.211942903347182	0.0
17	6.004275043831208E-4	0.0	0.0	17.085164637221702	0.0
18	0.0010007125073052013	0.0	0.0	18.03524109165726	0.0
19	0.0010007125073052013	0.0	0.0	18.773766922048498	0.0
20	0.0010007125073052013	0.0	0.0	19.319955808535678	0.0
21	0.0010007125073052013	0.0	0.0	19.933592718015227	0.0
22	0.0010007125073052013	0.0	0.0	20.56544259512773	0.0
23	0.0010007125073052013	0.0	0.0	21.191488339697866	0.0
24	0.0012008550087662417	0.0	0.0	21.676633763239426	0.0
25	0.0012008550087662417	0.0	0.0	22.117547693958098	0.0
26	0.0012008550087662417	0.0	0.0	22.55846162467677	0.0
27	0.0012008550087662417	0.0	0.0	23.05681645331476	0.0
28	0.0012008550087662417	0.0	0.0	23.48392055143262	0.0
29	0.0012008550087662417	0.0	0.0	23.937243317241876	0.0
30	0.0012008550087662417	0.0	0.0	24.52085885150227	0.0
31	0.0014009975102272818	0.0	0.0	24.956969362185877	0.0
32	0.0014009975102272818	0.0	0.0	25.40048514542354	0.0
33	0.0014009975102272818	0.0	0.0	25.87942615141981	0.0
34	0.0014009975102272818	0.0	0.0	26.353964022383938	0.0
35	0.0014009975102272818	0.0	0.0	26.878337376211864	0.0
36	0.0014009975102272818	0.0	0.0	27.310044751863327	0.0
37	0.0014009975102272818	0.0	0.0	27.77437535525294	0.0
38	0.0014009975102272818	0.0	0.0	28.265324911336872	0.0
39	0.0014009975102272818	0.0	0.0	28.841134888040283	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.8876286E-5	45.0	25
ATCTCGA	20	7.0294546E-4	45.0	42
GTCGATG	20	7.0294546E-4	45.0	1
CGATTGT	20	7.0294546E-4	45.0	10
TACGGGT	40	6.8012014E-9	45.0	4
GCGCAAC	25	3.8876286E-5	45.0	24
GTTACGG	35	1.2100827E-7	45.0	2
GTACGAG	25	3.8876286E-5	45.0	1
CCCCTCG	30	2.1630294E-6	44.999996	40
TAATACG	270	0.0	43.333332	4
TCGAATG	90	0.0	42.500004	44
CCGATGA	690	0.0	42.3913	18
CGATGAA	705	0.0	41.808514	19
CGAACAC	70	0.0	41.785713	34
ATAATAC	350	0.0	41.785713	3
CGTTATT	70	0.0	41.785713	1
AACGGGA	220	0.0	40.909092	4
ACGGGAT	105	0.0	40.714287	5
ACAACGG	50	1.0786607E-9	40.5	2
TTACGGG	100	0.0	40.5	3
>>END_MODULE