##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936432.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	706142
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09116579951341	33.0	31.0	34.0	30.0	34.0
2	32.1968796644301	33.0	31.0	34.0	30.0	34.0
3	32.14179017817946	33.0	31.0	34.0	30.0	34.0
4	35.7707826471163	37.0	35.0	37.0	35.0	37.0
5	32.1135196603516	37.0	35.0	37.0	0.0	37.0
6	33.890363411325204	37.0	35.0	37.0	17.0	37.0
7	35.532034633260736	37.0	35.0	37.0	32.0	37.0
8	35.78534487397719	37.0	35.0	37.0	35.0	37.0
9	37.724122060435434	39.0	38.0	39.0	35.0	39.0
10	37.320697536756064	39.0	37.0	39.0	34.0	39.0
11	37.295764874487	39.0	37.0	39.0	34.0	39.0
12	37.27229509078911	39.0	37.0	39.0	34.0	39.0
13	37.24484027291961	39.0	37.0	39.0	34.0	39.0
14	38.4205230675983	40.0	38.0	41.0	34.0	41.0
15	38.55590801850053	40.0	38.0	41.0	34.0	41.0
16	38.51802470324665	40.0	38.0	41.0	34.0	41.0
17	38.51024723072696	40.0	38.0	41.0	34.0	41.0
18	38.42469786530188	40.0	38.0	41.0	34.0	41.0
19	38.289664118548394	40.0	37.0	41.0	34.0	41.0
20	38.331287474757204	40.0	37.0	41.0	34.0	41.0
21	38.2823157381943	40.0	37.0	41.0	34.0	41.0
22	38.3704679795282	40.0	37.0	41.0	34.0	41.0
23	38.36465611732484	40.0	37.0	41.0	34.0	41.0
24	38.33646207136808	40.0	37.0	41.0	34.0	41.0
25	38.09482936859725	40.0	37.0	41.0	34.0	41.0
26	38.141713706308366	40.0	37.0	41.0	34.0	41.0
27	38.11875231893868	40.0	37.0	41.0	34.0	41.0
28	38.03511333414526	40.0	37.0	41.0	34.0	41.0
29	37.99562410959835	40.0	37.0	41.0	34.0	41.0
30	37.850724075327626	40.0	36.0	41.0	33.0	41.0
31	37.84375238974597	40.0	37.0	41.0	33.0	41.0
32	37.73781760609056	40.0	36.0	41.0	33.0	41.0
33	37.67010742881743	40.0	36.0	41.0	33.0	41.0
34	37.54174231245273	40.0	36.0	41.0	33.0	41.0
35	37.47409302944733	40.0	36.0	41.0	33.0	41.0
36	37.37095654981576	40.0	36.0	41.0	33.0	41.0
37	37.34505524384614	40.0	36.0	41.0	33.0	41.0
38	37.20852038258594	40.0	35.0	41.0	32.0	41.0
39	37.143563192672296	40.0	35.0	41.0	32.0	41.0
40	37.0489646558341	40.0	35.0	41.0	32.0	41.0
41	36.93159166286667	39.0	35.0	41.0	31.0	41.0
42	36.90630496415735	39.0	35.0	41.0	31.0	41.0
43	36.87685054847325	39.0	35.0	41.0	31.0	41.0
44	36.862050409124514	39.0	35.0	41.0	31.0	41.0
45	36.83560671932841	39.0	35.0	41.0	31.0	41.0
46	36.71912589819045	39.0	35.0	41.0	31.0	41.0
47	36.61710675756434	39.0	35.0	41.0	31.0	41.0
48	36.576024369036254	39.0	35.0	41.0	31.0	41.0
49	36.5399183167125	39.0	35.0	41.0	31.0	41.0
50	36.433037547688706	39.0	35.0	41.0	31.0	41.0
51	35.41809720990962	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	18.0
9	32.0
10	39.0
11	37.0
12	20.0
13	23.0
14	13.0
15	24.0
16	38.0
17	80.0
18	167.0
19	272.0
20	582.0
21	1004.0
22	1584.0
23	2319.0
24	3158.0
25	4327.0
26	5358.0
27	5813.0
28	6633.0
29	7389.0
30	9273.0
31	12103.0
32	15880.0
33	23512.0
34	40138.0
35	50249.0
36	52816.0
37	84287.0
38	140888.0
39	237858.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.680188970490356	19.198971311719173	22.584975826391858	12.535863891398613
2	34.16182580840681	23.974922890863308	24.402457296124574	17.460794004605308
3	32.60420708582693	24.82163644139564	26.159894185588733	16.4142622871887
4	27.966329718385253	25.40792078647071	27.596857289327076	19.02889220581696
5	23.269115843555547	36.70380744949316	22.235046208836184	17.79203049811511
6	24.144577153037208	36.14428825930195	25.07625944923259	14.63487513842825
7	80.05075466407607	5.102373177066369	10.50199534937732	4.344876809480247
8	81.07986778863174	5.829139181637688	8.682531275579134	4.408461754151431
9	75.52121244735478	6.24520280623444	10.600559094346462	7.633025652064315
10	37.86377244236995	29.444502663770177	17.914951950174327	14.776772943685549
11	28.08302012909585	25.13163074848968	27.292102721548922	19.493246400865548
12	24.5143328112476	22.67660045713185	31.535441879961823	21.27362485165873
13	24.858597845759068	23.39840995153949	33.297268821285236	18.4457233814162
14	21.014186948234208	27.89623050321324	30.88769680885714	20.201885739695417
15	19.828731331658506	25.252569596483426	34.80886280663096	20.109836265227106
16	22.80235420071317	25.769462799267	30.302403765814805	21.12577923420502
17	23.436362657935657	24.446074585564944	29.123887263468255	22.993675493031144
18	24.247955793593924	23.409597503051792	30.708837599236418	21.633609104117866
19	23.10767522679574	26.058922992825806	28.54723270956833	22.286169070810118
20	23.977755182385412	26.03201622336584	29.74784108578728	20.24238750846147
21	23.40251677424654	27.208691736223027	29.964511387228065	19.42428010230237
22	22.92060237176092	23.808100920211515	29.700683431944285	23.57061327608328
23	20.920013255124324	26.952652582625024	29.730564107502456	22.396770054748195
24	21.511962183243597	25.424489691875007	30.136148253467432	22.927399871413964
25	21.974617003378924	27.80673009111482	28.2291663716363	21.989486533869957
26	21.010788198407685	25.96928096615128	29.494350994559166	23.525579840881864
27	22.61089129381909	24.900232531133966	29.998782114645493	22.49009406040145
28	19.852097736715844	25.617227129954035	30.925649515253305	23.605025618076816
29	21.582203012991723	24.899099614525124	30.376893032846088	23.14180433963707
30	22.57194728539019	24.81583024377533	30.770156710689918	21.84206576014456
31	23.076236790900413	24.588680463702765	28.278306629544765	24.056776115852053
32	22.72305003809432	25.080507886515747	28.89277227526475	23.30366980012519
33	23.1593645470741	23.538749996459636	28.832019622115666	24.4698658343506
34	22.480181040074093	23.58109275471506	30.459879174443667	23.478847030767184
35	22.095131007644355	24.21141923295881	30.74990582630689	22.943543933089945
36	23.057260437702332	24.858597845759068	30.593563334286873	21.49057838225173
37	21.490011923947307	25.15528038269923	29.870196079542076	23.48451161381139
38	22.094139705611617	25.781783267388146	28.334952459986802	23.789124567013435
39	23.33879021499925	23.795922066666478	28.95451623044657	23.910771487887704
40	24.350767975846217	22.893695602300955	30.916019724078158	21.83951669777467
41	21.5676167116529	23.888962843167523	30.344463294918018	24.198957150261563
42	21.35760229528905	23.86106477167482	31.905763996476626	22.8755689365595
43	21.94586924442959	23.76929852635872	30.608999323082326	23.67583290612936
44	22.179250065850777	23.690985665772608	29.946243106910508	24.183521161466107
45	22.401018492031348	23.23427865783369	28.67298645314965	25.69171639698531
46	23.620319992296167	24.40628088967941	28.851420819042062	23.121978298982356
47	21.05185642547816	24.68356222969318	31.451039592603188	22.813541752225472
48	21.119831422008605	24.731286341840594	29.890022120196786	24.258860115954015
49	22.197235117016124	23.042815750939614	31.46831657088801	23.291632561156256
50	21.550339733368077	23.621311294328905	30.734753066663643	24.093595905639376
51	21.65088608240269	22.609758377210248	29.57167255311255	26.167682987274514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	200.0
1	598.0
2	996.0
3	3136.0
4	5276.0
5	3409.0
6	1542.0
7	1532.5
8	1523.0
9	1579.0
10	1635.0
11	1634.0
12	1633.0
13	1623.0
14	1613.0
15	1565.5
16	1518.0
17	1492.5
18	1467.0
19	1608.5
20	1750.0
21	1870.5
22	1991.0
23	2419.0
24	2847.0
25	3444.0
26	4781.5
27	5522.0
28	6511.0
29	7500.0
30	8889.0
31	10278.0
32	12838.0
33	15398.0
34	17248.5
35	19099.0
36	20995.5
37	22892.0
38	24330.5
39	25769.0
40	29090.0
41	32411.0
42	35775.0
43	39139.0
44	43743.0
45	48347.0
46	58598.0
47	68849.0
48	69128.5
49	69408.0
50	68574.5
51	67741.0
52	60882.5
53	54024.0
54	49011.5
55	43999.0
56	40131.5
57	36264.0
58	32294.0
59	28324.0
60	25880.0
61	23436.0
62	20727.5
63	18019.0
64	15844.5
65	13670.0
66	11888.5
67	10107.0
68	8452.0
69	6797.0
70	5521.5
71	4246.0
72	3606.0
73	2966.0
74	2370.0
75	1544.0
76	1314.0
77	900.5
78	487.0
79	344.0
80	201.0
81	139.5
82	78.0
83	52.0
84	26.0
85	20.5
86	15.0
87	9.0
88	3.0
89	4.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	706142.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.62845862430135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8729631481886	24.888499305829846
2	9.704527733022195	6.72105674142688
3	3.8830707097492736	4.033942602233676
4	2.1565986328785454	2.987187461114382
5	1.482078438879796	2.5661045949359074
6	1.1482161898513574	2.38565740932124
7	0.9031784676280143	2.1892974737631614
8	0.8148204620726317	2.2572781325692985
9	0.7057167648940926	2.1994095414249797
>10	7.24266963546537	44.02608208042964
>50	0.05202887528920852	1.2039544788454866
>100	0.029968632071060448	2.2144806136790374
>500	0.0016649240039478027	0.3975226199249404
>1k	0.002497386005921704	1.929526944501515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2944	0.41691331205338306	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	2797	0.3960959693659349	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	2456	0.34780539891409945	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	2449	0.3468140968813638	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	1643	0.23267274854066178	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	1098	0.1554928045633881	TruSeq Adapter, Index 21 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG	815	0.11541587952564783	No Hit
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT	783	0.11088421309028496	Illumina Paired End PCR Primer 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11669041071059362	0.0
2	0.0	0.0	0.0	0.6116333541978809	0.0
3	0.0	0.0	0.0	0.9122810992689856	0.0
4	0.0	0.0	0.0	1.3027124855907168	0.0
5	0.0	0.0	0.0	2.5670474210569547	0.0
6	0.0	0.0	0.0	3.5024116962310696	0.0
7	0.0	0.0	0.0	4.290213583103681	0.0
8	0.0	0.0	0.0	5.440265555653112	0.0
9	0.0	0.0	0.0	6.013521359726514	0.0
10	1.4161457610508934E-4	0.0	0.0	7.360984051366439	0.0
11	1.4161457610508934E-4	0.0	0.0	8.463311911768454	0.0
12	1.4161457610508934E-4	0.0	0.0	9.762059189228228	0.0
13	1.4161457610508934E-4	0.0	0.0	10.173024689085198	0.0
14	1.4161457610508934E-4	0.0	0.0	10.377374522404843	0.0
15	1.4161457610508934E-4	0.0	0.0	10.701671901685497	0.0
16	1.4161457610508934E-4	0.0	0.0	11.264023383398806	0.0
17	1.4161457610508934E-4	0.0	0.0	11.93612616159356	0.0
18	1.4161457610508934E-4	0.0	0.0	12.710757892888399	0.0
19	1.4161457610508934E-4	0.0	0.0	13.204851148919056	0.0
20	1.4161457610508934E-4	0.0	0.0	13.639183053833365	0.0
21	1.4161457610508934E-4	0.0	0.0	14.153385579670944	0.0
22	1.4161457610508934E-4	0.0	0.0	14.711063780372786	0.0
23	1.4161457610508934E-4	0.0	0.0	15.246508492626129	0.0
24	1.4161457610508934E-4	0.0	0.0	15.686363366008537	0.0
25	1.4161457610508934E-4	0.0	0.0	16.080051887580684	0.0
26	1.4161457610508934E-4	0.0	0.0	16.483228585751874	0.0
27	1.4161457610508934E-4	0.0	0.0	16.898725752044207	0.0
28	1.4161457610508934E-4	0.0	0.0	17.29482172141014	0.0
29	1.4161457610508934E-4	0.0	0.0	17.71881576226878	0.0
30	1.4161457610508934E-4	0.0	0.0	18.25624307858759	0.0
31	1.4161457610508934E-4	0.0	0.0	18.70615258687346	0.0
32	1.4161457610508934E-4	0.0	0.0	19.18693407275024	0.0
33	1.4161457610508934E-4	0.0	0.0	19.634294518666216	0.0
34	1.4161457610508934E-4	0.0	0.0	20.09624126592102	0.0
35	1.4161457610508934E-4	0.0	0.0	20.5743320748518	0.0
36	1.4161457610508934E-4	0.0	0.0	20.994927365883914	0.0
37	1.4161457610508934E-4	0.0	0.0	21.423877916906232	0.0
38	1.4161457610508934E-4	0.0	0.0	21.88016008111683	0.0
39	4.24843728315268E-4	0.0	0.0	22.464603436702532	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCC	25	3.889271E-5	45.000004	23
CGGTAAT	20	7.031436E-4	45.0	6
TATGCGG	80	0.0	42.1875	2
TATACGG	65	0.0	41.538464	2
CGTTTTT	1375	0.0	40.745453	1
TACGGGA	315	0.0	40.714287	4
CGTTATT	175	0.0	39.857143	1
AGGGCGC	115	0.0	39.130432	6
ATTACGG	35	6.244867E-6	38.571426	2
CGTAAGG	130	0.0	38.076927	2
CGATGAA	405	0.0	37.777775	19
GTTGACG	30	1.1394762E-4	37.499996	1
ACGATAG	30	1.1394762E-4	37.499996	1
AGGTTCG	30	1.1394762E-4	37.499996	1
ACTACCG	30	1.1394762E-4	37.499996	1
CGAATAT	30	1.1394762E-4	37.499996	14
CCGACGG	30	1.1394762E-4	37.499996	2
CCCATCG	30	1.1394762E-4	37.499996	40
AGGGTAC	175	0.0	37.285713	6
TTACGGG	145	0.0	37.24138	3
>>END_MODULE