##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936431.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	152086
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01852241494944	33.0	31.0	34.0	30.0	34.0
2	32.12272003997738	33.0	31.0	34.0	30.0	34.0
3	32.080263798114224	33.0	31.0	34.0	30.0	34.0
4	35.72993569427824	37.0	35.0	37.0	33.0	37.0
5	32.139749878358295	37.0	35.0	37.0	0.0	37.0
6	33.88950330733927	37.0	35.0	37.0	17.0	37.0
7	35.51564246544718	37.0	35.0	37.0	32.0	37.0
8	35.7482477019581	37.0	35.0	37.0	35.0	37.0
9	37.656990124008786	39.0	37.0	39.0	35.0	39.0
10	37.32123929881777	39.0	37.0	39.0	34.0	39.0
11	37.24567678813303	39.0	37.0	39.0	34.0	39.0
12	36.97919598122115	39.0	35.0	39.0	34.0	39.0
13	36.719809844430124	39.0	35.0	39.0	33.0	39.0
14	37.846290914351094	40.0	36.0	41.0	33.0	41.0
15	38.076529069079335	40.0	36.0	41.0	33.0	41.0
16	38.07537840432387	40.0	36.0	41.0	34.0	41.0
17	38.08145391423274	40.0	36.0	41.0	34.0	41.0
18	38.036071696277105	40.0	36.0	41.0	34.0	41.0
19	37.901831858290706	40.0	36.0	41.0	34.0	41.0
20	37.98350933024736	40.0	36.0	41.0	34.0	41.0
21	37.893645700458954	40.0	35.0	41.0	34.0	41.0
22	37.97039832726221	40.0	35.0	41.0	34.0	41.0
23	37.99458858803572	40.0	35.0	41.0	34.0	41.0
24	37.94601738490065	40.0	35.0	41.0	34.0	41.0
25	37.691740199623894	40.0	35.0	41.0	34.0	41.0
26	37.71533211472456	40.0	35.0	41.0	34.0	41.0
27	37.67611745985824	40.0	35.0	41.0	34.0	41.0
28	37.638369080651735	40.0	35.0	41.0	33.0	41.0
29	37.603730783898584	40.0	35.0	41.0	33.0	41.0
30	37.46885314887629	39.0	35.0	41.0	33.0	41.0
31	37.43600988914167	39.0	35.0	41.0	33.0	41.0
32	37.35439159422958	39.0	35.0	41.0	33.0	41.0
33	37.287061267966806	39.0	35.0	41.0	33.0	41.0
34	37.18313980248018	39.0	35.0	41.0	33.0	41.0
35	37.18889970148469	39.0	35.0	41.0	33.0	41.0
36	37.109365753586786	39.0	35.0	41.0	33.0	41.0
37	37.039786699630476	39.0	35.0	41.0	33.0	41.0
38	36.94079007929724	39.0	35.0	41.0	32.0	41.0
39	36.89409281590679	39.0	35.0	41.0	32.0	41.0
40	36.85303709743172	39.0	35.0	41.0	32.0	41.0
41	36.74533487631998	39.0	35.0	41.0	32.0	41.0
42	36.774765593151244	39.0	35.0	41.0	32.0	41.0
43	36.68906408216404	39.0	35.0	41.0	32.0	41.0
44	36.64698920347698	39.0	35.0	41.0	32.0	41.0
45	36.62576437015899	39.0	35.0	41.0	32.0	41.0
46	36.50670673171758	39.0	35.0	41.0	31.0	41.0
47	36.4268637481425	38.0	35.0	41.0	31.0	41.0
48	36.41617900398459	38.0	35.0	41.0	31.0	41.0
49	36.39837328879713	38.0	35.0	41.0	31.0	41.0
50	36.287751666820085	38.0	35.0	40.0	31.0	41.0
51	35.296989860999695	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	7.0
10	7.0
11	9.0
12	4.0
13	6.0
14	5.0
15	12.0
16	14.0
17	15.0
18	35.0
19	63.0
20	122.0
21	204.0
22	332.0
23	446.0
24	638.0
25	861.0
26	1100.0
27	1229.0
28	1400.0
29	1667.0
30	2048.0
31	2869.0
32	3926.0
33	6098.0
34	11370.0
35	16687.0
36	10990.0
37	16420.0
38	27241.0
39	46222.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55627736938311	19.980142813934222	23.945662322633247	12.517917494049419
2	32.53685414831082	25.07462882842602	24.34083347579659	18.047683547466566
3	32.797890667122545	24.066646502636665	26.430440671725208	16.705022158515575
4	28.80278263614008	25.43823889115369	25.922833133884776	19.836145338821456
5	23.136909380219087	36.441224044290735	22.522125639440844	17.89974093604934
6	24.45129729232145	36.927133332456634	23.916073800349803	14.705495574872113
7	78.70612679668082	5.780939731467722	10.494062569861788	5.018870901989664
8	78.74886577331247	6.0932630222374184	9.308549110371764	5.84932209407835
9	73.30523519587602	7.0354930762857855	11.619083939350103	8.040187788488092
10	42.39048959141538	25.128545691253635	17.854371868548057	14.626592848782927
11	33.48500190681588	24.28691661296898	24.413160974711676	17.814920505503466
12	31.103454624357273	21.470746814302437	28.505582367870808	18.920216193469486
13	24.409215838407217	28.67259313809292	29.007929723971966	17.9102612995279
14	18.538195494654342	32.64403035124864	28.178793577318096	20.638980576778927
15	17.27509435450995	25.610509843115082	38.084373315098034	19.030022487276934
16	20.183317333613875	23.51893007903423	34.45813552858251	21.83961705876938
17	19.674394750338625	22.94622779217022	29.25187065213103	28.127506805360124
18	22.038846442144578	23.362439672290677	32.05423247373197	22.544481411832777
19	23.619531054797942	25.749247136488567	28.7975224544008	21.833699354312692
20	26.490932761726917	24.829372854832137	27.848717173178333	20.830977210262613
21	22.292650211064792	27.38910879370882	29.171652880607024	21.146588114619362
22	20.73892402982523	24.813592309614297	27.14516786554976	27.302315795010717
23	19.752639953710403	28.60552582091711	27.381876043817314	24.25995818155517
24	21.470746814302437	23.667530213168863	31.319122075667714	23.54260089686099
25	18.588167221177493	26.435043330747078	30.342043317596623	24.634746130478806
26	19.310127164893547	28.62590902515682	29.023710269189802	23.040253540759835
27	20.191207606222793	29.96857041410781	27.18527675131176	22.65494522835764
28	18.02532777507463	26.821009165866684	31.933905816445957	23.219757242612733
29	19.193745643911996	24.933261444182897	31.266520258274923	24.60647265363018
30	20.75075943873861	27.886853490788106	28.40629643754192	22.956090632931367
31	24.39409281590679	27.378588430230266	24.100837683941982	24.126481069920967
32	24.62356824428284	28.249806030798364	23.94434727719843	23.18227844772037
33	22.825901134884212	27.853319832200203	24.61041778993464	24.710361242980944
34	20.471312283839406	28.409584051128967	26.51986376129295	24.599239903738674
35	18.760438173138883	27.348342385229408	26.55339741988086	27.337822021750853
36	23.270386491853294	29.299212287784542	24.589377062977526	22.841024157384638
37	20.66922662177978	29.426771694962063	27.35952027142538	22.544481411832777
38	19.6263955919677	28.92179424799127	24.223794432097627	27.2280157279434
39	20.012361427087306	26.625067396078535	26.94528095945715	26.41729021737701
40	21.522033586260406	25.121970464079535	26.915034914456292	26.440961035203763
41	17.740620438436146	27.630419630998247	26.90319950554292	27.725760425022685
42	21.712057651591863	26.672409031732048	26.44885130781269	25.166682008863404
43	22.59379561563852	26.690162145102114	25.78738345409834	24.92865878516103
44	20.946701208526754	26.530384124771512	26.850597688150124	25.672316978551606
45	19.75855765816709	27.00117039043699	25.283063529844956	27.957208421550966
46	21.809371013768526	27.052457162394962	25.879436634535725	25.25873518930079
47	19.031995055429167	27.475901792406926	28.553581526241732	24.938521625922174
48	20.15504385676525	25.265967939192297	28.17156082742659	26.40742737661586
49	21.337269702668227	23.355864445116577	30.068513867154113	25.238351985061087
50	19.46793261707192	25.191667872124984	29.02765540549426	26.312744105308838
51	19.05829596412556	25.542785003221862	25.91823047486291	29.48068855778967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	59.0
1	188.5
2	318.0
3	472.0
4	626.0
5	446.0
6	266.0
7	264.5
8	263.0
9	300.0
10	337.0
11	343.5
12	350.0
13	312.0
14	274.0
15	297.0
16	320.0
17	319.5
18	319.0
19	303.5
20	288.0
21	320.0
22	352.0
23	494.5
24	637.0
25	622.0
26	853.5
27	1100.0
28	1320.5
29	1541.0
30	1866.5
31	2192.0
32	2200.0
33	2208.0
34	2573.5
35	2939.0
36	3485.5
37	4032.0
38	4561.0
39	5090.0
40	5758.0
41	6426.0
42	8173.0
43	9920.0
44	11238.5
45	12557.0
46	14272.0
47	15987.0
48	16743.0
49	17499.0
50	17002.0
51	16505.0
52	14070.0
53	11635.0
54	10300.5
55	8966.0
56	7700.5
57	6435.0
58	6020.0
59	5605.0
60	5270.0
61	4935.0
62	4328.5
63	3722.0
64	3374.5
65	3027.0
66	2360.5
67	1694.0
68	1400.5
69	1107.0
70	983.0
71	859.0
72	663.0
73	467.0
74	404.5
75	258.5
76	175.0
77	113.5
78	52.0
79	43.5
80	35.0
81	25.5
82	16.0
83	8.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	152086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.61828176163486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.87158837450836	28.527938140262748
2	10.4030102327482	8.034927606748814
3	3.7083070846032045	4.296253435556198
4	1.963121243593891	3.0324947726943963
5	1.2326971208690174	2.3802322370237894
6	0.8972809153286909	2.079086832450061
7	0.6725350314133451	1.8180503136383364
8	0.5669725707864403	1.7516405191799378
9	0.5652699504537484	1.964677879620741
>10	6.0545179030527985	42.12024775455992
>50	0.03745764731922429	0.9520928948095155
>100	0.02213406432499617	1.9147061530975895
>500	0.005107860998076039	1.1276514603579555
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	660	0.43396499349052503	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGC	551	0.36229501729284747	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	504	0.3313914495745828	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTC	495	0.3254737451178938	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCG	477	0.31363833620451587	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT	377	0.2478860644635272	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	315	0.20711965598411425	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	223	0.1466275659824047	No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	181	0.11901161185118944	No Hit
ACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	153	0.10060097576371264	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25709138250726565	0.0
2	0.0	0.0	0.0	1.280196730797049	0.0
3	0.0	0.0	0.0	1.6957510882000972	0.0
4	0.0	0.0	0.0	2.25924805702037	0.0
5	0.0	0.0	0.0	3.8379601015215075	0.0
6	0.0	0.0	0.0	4.732190997198953	0.0
7	0.0	0.0	0.0	5.6770511421169605	0.0
8	0.0	0.0	0.0	7.29850216324974	0.0
9	0.0	0.0	0.0	7.93695672185474	0.0
10	0.0	0.0	0.0	9.490682903094301	0.0
11	0.0	0.0	0.0	11.082545401943637	0.0
12	0.0	0.0	0.0	12.73950264981655	0.0
13	6.575227174098865E-4	0.0	0.0	13.281958891679707	0.0
14	6.575227174098865E-4	0.0	0.0	13.502229002012019	0.0
15	6.575227174098865E-4	0.0	0.0	13.96052233604671	0.0
16	6.575227174098865E-4	0.0	0.0	14.874478913246453	0.0
17	6.575227174098865E-4	0.0	0.0	15.944268374472339	0.0
18	6.575227174098865E-4	0.0	0.0	17.26325894559657	0.0
19	6.575227174098865E-4	0.0	0.0	17.95168523072472	0.0
20	6.575227174098865E-4	0.0	0.0	18.54148310824139	0.0
21	6.575227174098865E-4	0.0	0.0	19.28316873347974	0.0
22	6.575227174098865E-4	0.0	0.0	20.003156109043566	0.0
23	6.575227174098865E-4	0.0	0.0	20.80533382428363	0.0
24	6.575227174098865E-4	0.0	0.0	21.353707770603474	0.0
25	0.001315045434819773	0.0	0.0	21.875123285509513	0.0
26	0.001315045434819773	0.0	0.0	22.349197164762042	0.0
27	0.001315045434819773	0.0	0.0	22.83773654379759	0.0
28	0.001315045434819773	0.0	0.0	23.311152900332708	0.0
29	0.001315045434819773	0.0	0.0	23.80560998382494	0.0
30	0.001315045434819773	0.0	0.0	24.407243270254988	0.0
31	0.001315045434819773	0.0	0.0	24.95693226200965	0.0
32	0.001315045434819773	0.0	0.0	25.454019436371528	0.0
33	0.001315045434819773	0.0	0.0	25.89455965703615	0.0
34	0.001315045434819773	0.0	0.0	26.373236195310547	0.0
35	0.001315045434819773	0.0	0.0	26.958431413805346	0.0
36	0.001315045434819773	0.0	0.0	27.444340701971253	0.0
37	0.001315045434819773	0.0	0.0	27.92433228568047	0.0
38	0.001315045434819773	0.0	0.0	28.37670791525847	0.0
39	0.001315045434819773	0.0	0.0	28.84880922635877	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTAG	25	3.8748018E-5	45.000004	1
CTAGATA	25	3.8748018E-5	45.000004	19
TTAGCTA	20	7.0139684E-4	45.000004	24
CAACGAG	25	3.8748018E-5	45.000004	14
ACGCAGG	25	3.8748018E-5	45.000004	2
TAGACAC	25	3.8748018E-5	45.000004	25
GACACCA	25	3.8748018E-5	45.000004	27
AGGGTAC	20	7.0139684E-4	45.000004	6
AGTATCG	25	3.8748018E-5	45.000004	34
CTTGCGG	20	7.0139684E-4	45.000004	2
ACAATTA	20	7.0139684E-4	45.000004	43
AATCTTC	20	7.0139684E-4	45.000004	35
CGGGATA	40	6.7484507E-9	45.000004	6
ATAAGCG	20	7.0139684E-4	45.000004	32
AACGAGC	25	3.8748018E-5	45.000004	15
CATCGTT	20	7.0139684E-4	45.000004	36
GGCATTA	20	7.0139684E-4	45.000004	8
CATAACA	30	2.1530504E-6	45.000004	27
TAGATAC	25	3.8748018E-5	45.000004	20
TTACGGG	20	7.0139684E-4	45.000004	3
>>END_MODULE