##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936430.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	192899
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.911565119570344	33.0	31.0	34.0	30.0	34.0
2	32.02597214086128	33.0	31.0	34.0	30.0	34.0
3	32.00843446570485	33.0	31.0	34.0	30.0	34.0
4	35.65911176315066	37.0	35.0	37.0	33.0	37.0
5	31.70013323034334	37.0	35.0	37.0	0.0	37.0
6	33.64789864125786	37.0	35.0	37.0	17.0	37.0
7	35.378856292671294	37.0	35.0	37.0	32.0	37.0
8	35.646250110161276	37.0	35.0	37.0	33.0	37.0
9	37.60615140565788	39.0	37.0	39.0	35.0	39.0
10	37.226750786681116	39.0	37.0	39.0	34.0	39.0
11	37.19494139420111	39.0	37.0	39.0	34.0	39.0
12	36.983789444216924	39.0	35.0	39.0	33.0	39.0
13	36.73757769610003	39.0	35.0	39.0	33.0	39.0
14	37.88365414024956	40.0	36.0	41.0	33.0	41.0
15	38.02912404937299	40.0	36.0	41.0	33.0	41.0
16	38.07186662450298	40.0	36.0	41.0	34.0	41.0
17	38.07235392614788	40.0	36.0	41.0	34.0	41.0
18	38.04493543253205	40.0	36.0	41.0	34.0	41.0
19	37.93120752310795	40.0	36.0	41.0	34.0	41.0
20	37.91017579147637	40.0	36.0	41.0	34.0	41.0
21	37.90676467996205	40.0	36.0	41.0	34.0	41.0
22	37.98927936381215	40.0	36.0	41.0	34.0	41.0
23	37.97961627587494	40.0	36.0	41.0	34.0	41.0
24	37.983250301971495	40.0	35.0	41.0	34.0	41.0
25	37.782326502470205	40.0	35.0	41.0	34.0	41.0
26	37.797028496778104	40.0	35.0	41.0	34.0	41.0
27	37.703057040212755	40.0	35.0	41.0	33.0	41.0
28	37.71538991907682	40.0	36.0	41.0	33.0	41.0
29	37.69394346264107	40.0	36.0	41.0	33.0	41.0
30	37.56459598027983	40.0	36.0	41.0	33.0	41.0
31	37.54411375901378	40.0	35.0	41.0	33.0	41.0
32	37.413833145843164	40.0	35.0	41.0	33.0	41.0
33	37.347762300478486	40.0	35.0	41.0	33.0	41.0
34	37.24902669272521	40.0	35.0	41.0	33.0	41.0
35	37.222173261655065	40.0	35.0	41.0	33.0	41.0
36	37.11686426575565	39.0	35.0	41.0	33.0	41.0
37	36.9377238865935	39.0	35.0	41.0	32.0	41.0
38	36.82372122198664	39.0	35.0	41.0	32.0	41.0
39	36.818511241634226	39.0	35.0	41.0	31.0	41.0
40	36.687219736753434	39.0	35.0	41.0	31.0	41.0
41	36.64148595897335	39.0	35.0	41.0	31.0	41.0
42	36.61629661117994	39.0	35.0	41.0	31.0	41.0
43	36.53675239373973	39.0	35.0	41.0	31.0	41.0
44	36.508411137434614	39.0	35.0	41.0	31.0	41.0
45	36.588898853804324	39.0	35.0	41.0	31.0	41.0
46	36.512895349379725	39.0	35.0	41.0	31.0	41.0
47	36.413796857422795	39.0	35.0	41.0	31.0	41.0
48	36.37407658930321	39.0	35.0	41.0	31.0	41.0
49	36.35795934660107	39.0	35.0	41.0	31.0	41.0
50	36.18771481448841	38.0	35.0	40.0	31.0	41.0
51	35.1481708044106	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	14.0
10	8.0
11	3.0
12	5.0
13	4.0
14	4.0
15	3.0
16	6.0
17	13.0
18	35.0
19	88.0
20	229.0
21	381.0
22	555.0
23	725.0
24	987.0
25	1144.0
26	1447.0
27	1523.0
28	1818.0
29	2131.0
30	2741.0
31	3538.0
32	5103.0
33	7775.0
34	13546.0
35	20130.0
36	14019.0
37	21108.0
38	36092.0
39	57684.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57255351245989	19.64966122167559	23.793280421360404	10.98450484450412
2	30.458944836416986	28.154630143235583	24.95865712108409	16.427767899263344
3	30.23914069020576	27.616006303817027	25.954515057102423	16.1903379488748
4	27.544984681102548	24.268658728142707	30.404512205869395	17.781844384885353
5	25.217860123691672	36.398840844172334	21.450603683793073	16.932695348342914
6	22.044178559764436	37.68811657914245	24.749221094977162	15.518483766115947
7	76.73601210996428	5.172655120036911	13.20898501288239	4.882347757116419
8	77.19480142457971	7.841409234884576	9.11461438369302	5.8491749568427
9	72.86714809304351	5.409048258415026	11.16335491630335	10.560448732238115
10	40.73167823576068	24.604585819522136	18.53405149845256	16.129684446264626
11	33.139103883379384	23.941026132846723	24.96332277513103	17.956547208642863
12	28.103826354724493	21.648116371780052	30.459463242422203	19.788594031073256
13	23.388923737292572	27.779304195459797	32.380157491744384	16.451614575503243
14	19.067491277818963	34.666846380748474	27.6699205283594	18.595741813073165
15	15.869963037651827	25.41848324770994	40.49061944333563	18.2209342713026
16	16.917661574191676	24.874675348239233	35.11423076324916	23.093432314319926
17	17.999574907075726	24.448027205947152	29.655415528333478	27.89698235864364
18	19.83214013551133	24.978356549282267	32.25574005049274	22.933763264713658
19	22.117792212504988	25.956070275118066	28.626898013986597	23.29923949839035
20	23.719148362614632	26.20542356362656	29.568841725462548	20.506586348296256
21	20.34484367466913	30.179005593600795	29.778277751569476	19.697872980160604
22	18.353127802632464	25.057672668080187	29.036438758106577	27.552760771180772
23	17.474948029797975	28.928610309021817	28.867956806411645	24.728484854768556
24	20.90420375429629	25.123510230742514	30.078434828589057	23.893851186372142
25	17.316315792202136	29.20803114583279	29.68548307663596	23.79016998532911
26	17.369193204734085	26.88971949051058	31.883524538748258	23.85756276600708
27	19.977293816971574	30.41280670195284	28.59423843565804	21.01566104541755
28	17.42207061726603	26.539277030985126	34.33040088336383	21.708251468385008
29	18.724306502366524	23.704114588463394	33.42474559225294	24.146833316917142
30	20.367653538898594	30.36303972545218	29.77309369151732	19.496213044131903
31	26.14010440696945	26.605632999652666	24.384263267305688	22.869999326072193
32	23.605099041467295	29.928097087076655	24.66938657017403	21.797417301282017
33	23.93584207279457	28.335035433050454	24.16445912109446	23.56466337306051
34	23.606654259482944	25.013089751631686	28.48278114453678	22.897474844348597
35	19.61544642533139	28.710879786831452	26.68857796048709	24.985095827350065
36	27.44804275812731	26.793814379545772	27.020876209830014	18.737266652496903
37	22.922358332598925	27.815074209819645	28.03954401007781	21.223023447503618
38	21.92805561459624	31.042151592284046	22.765799719023946	24.263993074095772
39	25.2188969357021	25.43040658582989	25.900600832560045	23.45009564590796
40	22.822824379597613	27.458929284236827	27.557944831232923	22.160301504932633
41	22.10172162634332	26.28370287041405	25.690127994442687	25.924447508799943
42	24.22666784172028	25.30184189653653	27.77723057143894	22.694259690304253
43	25.6460634839994	24.90629811455736	26.396196973545745	23.0514414278975
44	22.184666587177745	26.06804597224454	27.761678391282484	23.985609049295228
45	21.16703559894038	25.614440717681276	25.99339550749356	27.225128175884787
46	25.55275040306067	27.11626291478961	26.217865307751726	21.113121374398
47	20.837329379623533	25.706198580604354	30.868485580536962	22.587986459235143
48	22.62479328560542	24.98665104536571	27.887132644544554	24.501423024484314
49	23.387886925282142	20.951897106776084	32.13080420323589	23.52941176470588
50	22.173261655063012	22.67974432215823	30.347487545295728	24.799506477483035
51	20.786007185107234	23.934286854778925	25.863794006189767	29.415911953924073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	80.0
1	134.0
2	188.0
3	489.5
4	791.0
5	538.0
6	285.0
7	298.0
8	311.0
9	338.0
10	365.0
11	368.0
12	371.0
13	405.5
14	440.0
15	422.5
16	405.0
17	361.0
18	317.0
19	324.0
20	331.0
21	369.0
22	407.0
23	469.5
24	532.0
25	628.0
26	1050.0
27	1376.0
28	1680.5
29	1985.0
30	2254.5
31	2524.0
32	2715.5
33	2907.0
34	3188.0
35	3469.0
36	4293.0
37	5117.0
38	5678.5
39	6240.0
40	7255.0
41	8270.0
42	10201.0
43	12132.0
44	15021.5
45	17911.0
46	23072.5
47	28234.0
48	26562.5
49	24891.0
50	23835.5
51	22780.0
52	19187.5
53	15595.0
54	12938.0
55	10281.0
56	8715.5
57	7150.0
58	6407.5
59	5665.0
60	4755.0
61	3845.0
62	3277.0
63	2709.0
64	2220.0
65	1731.0
66	1359.5
67	988.0
68	755.5
69	523.0
70	471.0
71	419.0
72	361.0
73	303.0
74	225.5
75	110.5
76	73.0
77	55.0
78	37.0
79	25.5
80	14.0
81	18.0
82	22.0
83	16.5
84	11.0
85	6.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	192899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.23089285066278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.06046831227866	29.054582968289104
2	10.973095829589303	8.60968693461345
3	4.103018129922301	4.828951938579257
4	2.099741001109995	3.2949885691475846
5	1.3121729478302235	2.5738858158932914
6	0.891960463026587	2.099544321121416
7	0.7228183307785824	1.9849765939688644
8	0.5391405465405149	1.6920772010222966
9	0.4809979385802632	1.6982980730848787
>10	4.697658438606692	34.79385585202619
>50	0.0726782599503145	1.9398752715151453
>100	0.03832126433743855	3.157092571760351
>500	0.002642845816375073	0.6200135822373366
>1k	0.005285691632750146	3.652170306740833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGC	2075	1.0756924608214662	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	1904	0.987045033929673	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCG	1752	0.9082473211369682	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	1314	0.6811854908527261	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	645	0.33437187336378105	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	551	0.28564170887355556	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCT	477	0.24727966448763344	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	427	0.2213593642268752	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCAGAGT	387	0.20062312401826865	No Hit
TCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	331	0.1715923877262194	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT	317	0.16433470365320713	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTC	311	0.16122426762191613	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTA	305	0.15811383159062514	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	290	0.15033774151239768	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGC	237	0.12286222323599397	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	231	0.11975178720470298	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCG	226	0.11715975717862717	No Hit
ACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	213	0.11042047911083003	No Hit
GAATATGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	202	0.10471801305346322	No Hit
GTCGAAGGGATTCAGTTAGCACTATTGTTGTAATTTGTACTGAAACCCATA	193	0.10005235900652674	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3115620091343138	0.0
2	0.0	0.0	0.0	1.7164422832674093	0.0
3	0.0	0.0	0.0	2.235366694487789	0.0
4	0.0	0.0	0.0	3.0036443942166624	0.0
5	0.0	0.0	0.0	5.609152976428079	0.0
6	0.0	0.0	0.0	6.803042006438603	0.0
7	0.0	0.0	0.0	8.135345439841576	0.0
8	0.0	0.0	0.0	10.101141011617479	0.0
9	0.0	0.0	0.0	10.787510562522357	0.0
10	0.0	0.0	0.0	13.310074183899346	0.0
11	0.0	0.0	0.0	15.239581335310188	0.0
12	0.0	0.0	0.0	17.94306865250727	0.0
13	0.0	0.0	0.0	18.73311940445518	0.0
14	0.0	0.0	0.0	19.084080269985847	0.0
15	0.0	0.0	0.0	19.697354574155387	0.0
16	0.0	0.0	0.0	20.780304719049866	0.0
17	0.0	0.0	0.0	21.958641568903936	0.0
18	0.0	0.0	0.0	23.469276668100925	0.0
19	0.0	0.0	0.0	24.49883099445824	0.0
20	0.0	0.0	0.0	25.253111732046303	0.0
21	0.0	0.0	0.0	26.146843685037247	0.0
22	0.0	0.0	0.0	27.0032504056527	0.0
23	0.0	0.0	0.0	27.95089658318602	0.0
24	0.0	0.0	0.0	28.625861201976164	0.0
25	0.0	0.0	0.0	29.183147657582467	0.0
26	0.0	0.0	0.0	29.706219316844567	0.0
27	0.0	0.0	0.0	30.33815623720185	0.0
28	0.0	0.0	0.0	30.910994872964608	0.0
29	0.0	0.0	0.0	31.490572786795163	0.0
30	0.0	0.0	0.0	32.184718427778265	0.0
31	0.0	0.0	0.0	32.823913032208566	0.0
32	0.0	0.0	0.0	33.40141732201826	0.0
33	0.0	0.0	0.0	34.02972540033904	0.0
34	0.0	0.0	0.0	34.609303314169594	0.0
35	0.0	0.0	0.0	35.23398255045387	0.0
36	0.0	0.0	0.0	35.76483029979419	0.0
37	5.184060052151645E-4	0.0	0.0	36.30293573320753	0.0
38	5.184060052151645E-4	0.0	0.0	36.86540624886599	0.0
39	5.184060052151645E-4	0.0	0.0	37.43306082457659	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAG	30	2.1560809E-6	45.000004	1
AAGCGGC	45	3.8198777E-10	45.000004	22
AAGTGCG	30	2.1560809E-6	45.000004	1
AGCACGG	30	2.1560809E-6	45.000004	2
CCTTATA	20	7.018676E-4	45.0	28
TAATACG	50	2.1827873E-11	45.0	4
CAGCGCG	20	7.018676E-4	45.0	1
CGGCGAA	35	1.2049168E-7	45.0	31
GAAGCGG	35	1.2049168E-7	45.0	2
CGAACGG	25	3.8787002E-5	45.0	2
CGAACCC	20	7.018676E-4	45.0	34
GTATTAG	20	7.018676E-4	45.0	1
CGCGGGT	25	3.8787002E-5	45.0	4
TGAAGCG	20	7.018676E-4	45.0	1
GCGAACC	25	3.8787002E-5	45.0	33
AACCCAT	20	7.018676E-4	45.0	44
TTATGCA	25	3.8787002E-5	45.0	14
CAGCACG	20	7.018676E-4	45.0	1
GGCCGGT	20	7.018676E-4	45.0	9
TTCACGG	20	7.018676E-4	45.0	2
>>END_MODULE